The potential of Mimosa pigra to restore contaminated soil with anthracene and phenanthrene

Hydrocarbon contamination cause serious environmental damage and human health problems, therefore, to resolve this problem has been proposed diversely remediation techniques friendly with the environment i.e. bioaugmentation, biostimulation, natural attenuation and phytoremediation. Among options, the phytoremediation causes minimum alterations to soil, low cost, it has proved good eff iciency and gained public acceptation. In the present study, the capacity of Mimosa pigra to restore a contaminated soil with both anthracene and phenanthrene was evaluated, along with the soil physicochemical changes due to both plant and contaminant presence in the soil. A silt-loam contaminated soil with 100 mg kg-1 (dry weight) anthracene and 200 mg kg-1 (dry weight) phenanthrene was used in all three treatments: Contaminated soil with M. pigra (C1), contaminated soil without M. pigra (C2), and not contaminated soil with M. pigra (C0) as control. After 70 days of experimentation both biomass and height of M. pigra was not affected due to soil contaminants. There was no difference among treatments for some physicochemical characteristics e.g. soil pH, electrolytic conductivity, soil-texture, and total nitrogen content throughout the experiment. M. pigra increased the soil water holding capacity after 50 and 70 days of the experiment, however, total organic carbon content was lower after 70 days compared to the control treatment. The higher contaminant removal rate was before 7 days of experimentation for all treatments. Anthracene and phenanthrene content were lower in treatments with the presence of M. pigra: 92% of phenanthrene and 80% of anthracene were removed in C1 while nearly 77% of phenanthrene and 60% of anthracene were removed in C2. This suggest a greater removal of contaminants using M. pigra. Total removal of contaminants was not achieved. However, M. pigra was able to accumulate both compounds, showing the M. pigra capability for bioremediation use in contaminated soils.

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Phytoremediation of hydrocarbon-contaminated soils with emphasis on the effect of petroleum hydrocarbons on the growth of plant species

Phytoprotection ◽

10.7202/000379ar ◽

2009 ◽

Vol 89 (1) ◽

pp. 21-29 ◽

Cited By ~ 41

Author(s):

Ravanbakhsh Shirdam ◽

Ali Daryabeigi Zand ◽

Gholamreza Nabi Bidhendi ◽

Nasser Mehrdadi

Keyword(s):

Plant Species ◽

Contaminated Soil ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Petroleum Hydrocarbon ◽

Green Technology ◽

Hydrocarbon Contamination ◽

Total Petroleum Hydrocarbons ◽

Control Treatment ◽

Polluted Soils

To date, many developing countries such as Iran have almost completely abandoned the idea of decontaminating oil-polluted soils due to the high costs of conventional (physical/chemical) soil remediation methods. Phytoremediation is an emerging green technology that can become a promising solution to the problem of decontaminating hydrocarbon-polluted soils. Screening the capacity of native tolerant plant species to grow on aged, petroleum hydrocarbon-contaminated soils is a key factor for successful phytoremediation. This study investigated the effect of hydrocarbon pollution with an initial concentration of 40 000 ppm on growth characteristics of sorghum (Sorghum bicolor) and common flax (Linum usitatissumum). At the end of the experiment, soil samples in which plant species had grown well were analyzed for total petroleum hydrocarbons (TPHs) removal by GC-FID. Common flax was used for the first time in the history of phytoremediation of oil-contaminated soil. Both species showed promising remediation efficiency in highly contaminated soil; however, petroleum hydrocarbon contamination reduced the growth of the surveyed plants significantly. Sorghum and common flax reduced TPHs concentration by 9500 and 18500 mg kg‑1, respectively, compared with the control treatment.

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Influence of Uniform Electric Field in Bioremediation of Oil-Contaminated Soils

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.777.253 ◽

2013 ◽

Vol 777 ◽

pp. 253-257

Author(s):

Jin Lan Xu ◽

Dong Dong Sun ◽

Ting Lin Huang ◽

Long Fei Han

Keyword(s):

Electric Field ◽

Direct Current ◽

Contaminated Soil ◽

Contaminated Soils ◽

Control Treatment ◽

Uniform Electric Field ◽

Direct Current Electric Field ◽

Inoculation Treatment ◽

Current Electric Field ◽

Electric Field Treatment

Electrokinetic remediation is an effective technology to enhance bioremediation of oil-contaminated soil as the transport process of non-polar organic contaminants in soil was promoted under low-power direct current electric fields. In this study three treatments including the application of electric field treatment alone, inoculation treatment alone, and combination of the application of electric field and inoculation treatment were carried out. The results indicated that highest removal efficiency was 83% in couple of the application of electric field and inoculation treatment. TPH concentration decreased from 6000 mg.kg-1 to 2000 mg.kg-1 when a 30 voltage direct current electric field was applied to oil-contaminated soil for 10 min after 4 days. It was observed that dehydrogenase activity had no increase after application direct current electric field. However, the TPH degradation efficiency was 52% higher than the control treatment. GC analysis showed that n-alkanes (C14-C31) were preferentially degraded, and soil remaindered more low quality compounds after the application of electric field treatment applied 52 days. These results indicated that the application of electric field improved degradation of inoculated bacteria through promoting the transport of nutrients, such as nitrogen and phosphorous and electron acceptors.

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Ornamental Plant Efficiency for Heavy Metals Phytoextraction from Contaminated Soils Amended with Organic Materials

Molecules ◽

10.3390/molecules26113360 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3360

Author(s):

Mahrous Awad ◽

M. A. El-Desoky ◽

A. Ghallab ◽

Jan Kubes ◽

S. E. Abdel-Mawly ◽

...

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Contaminated Soils ◽

Organic Materials ◽

Ornamental Plants ◽

Soil Health ◽

Dry Weight ◽

Control Treatment ◽

Chemical Agent ◽

Rf Values

Accumulation of heavy metals (HMs) by ornamental plants (OPs) from contaminated agriculture soils is a unique technique that can efficiently reduce the metal load in the food chain. Amaranthus tricolor L. has attractive characteristics acquiring a higher growth rate and large biomass when grown at heavy metal contaminated soils. Site-specific detailed information is not available on the use of A. tricolor plant in metal phytoremediation from the polluted sites. The study aimed to enhance the uptake of HMs (Pb, Zn, and Cu) via amending poultry litter extract (PLE), vinasse sugarcane (VSC), and humic acid (HA) as natural mobilized organic materials compared to ethylene diamine tetraacetic acid (EDTA), as a common mobilized chemical agent by A. tricolor plant. The studied soils collected from Helwan, El-Gabal El-Asfar (Cairo Governorate), Arab El-Madabeg (Assiut Governorate), Egypt, and study have been conducted under pot condition. Our results revealed all organic materials in all studied soils, except EDTA in EL-Gabal El-Asfar soil, significantly increased the dry weight of the A. tricolor plant compared to the control treatment. The uptake of Pb and Zn significantly (p > 0.05) increased due to applying all organic materials to the studied soils. HA application caused the highest uptake as shown in Pb concentration by more than 5 times in Helwan soil and EDTA by 65% in El-Gabal El-Asfar soil while VSC increased it by 110% in El-Madabeg soil. Also, an increase in Zn concentration due to EDTA application was 58, 42, and 56% for Helwan, El-Gabal El-Asfar, and El-Madabeg soil, respectively. In all studied soils, the application of organic materials increased the remediation factor (RF) than the control. El-Madabeg soil treated with vinasse sugarcane gave the highest RF values; 6.40, 3.26, and 4.02% for Pb, Zn, and Cu, respectively, than the control. Thus, we identified A. tricolor as a successful ornamental candidate that, along with organic mobilization amendments, most efficiently develop soil health, reduce metal toxicity, and recommend remediation of heavy metal-contaminated soils. Additionally, long-term application of organic mobilization amendments and continued growth of A. tricolor under field conditions could be recommended for future directions to confirm the results.

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Uso de Leersia hexandra (Poaceae) en la fitorremediación de suelos contaminados con petróleo fresco e intemperizado

Revista de Biología Tropical ◽

10.15517/rbt.v65i1.22967 ◽

2016 ◽

Vol 65 (1) ◽

pp. 21 ◽

Cited By ~ 3

Author(s):

Alfredo Arias Trinidad ◽

María Del Carmen Rivera Cruz ◽

Antonio Roldán Garrigós ◽

Lorenzo Armando Aceves Navarro ◽

Roberto Quintero-Lizaola ◽

...

Keyword(s):

Biomass Production ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Plant Biomass ◽

Soxhlet Extraction ◽

Dry Weight ◽

Total Petroleum Hydrocarbons ◽

Control Treatment ◽

Experimental Conditions ◽

Leersia Hexandra

The oil industry has generated chronic oil spills and their accumulation in wetlands of the state of Tabasco, in Southeastern Mexico. Waterlogging is a factor that limits the use of remediation technologies because of its high cost and low levels of oil degradation. However, Leersia hexandra is a grass that grows in these contaminated areas with weathered oil. The aim of the study was to evaluate the bacteria density, plant biomass production and phytoremediation of L. hexandra in contaminated soil. For this, two experiments in plastic tunnel were performed with fresh (E1) and weathered petroleum (E2) under waterlogging experimental conditions. The E1 was based on eight doses: 6 000, 10 000, 30 000, 60 000, 90 000, 120 000, 150 000 and 180 000 mg.kg-1 dry basis (d. b.) of total petroleum hydrocarbons fresh (TPH-F), and the E2, that evaluated five doses: 14 173, 28 400, 50 598, 75 492 and 112 142 mg. kg-1 d. b. of total petroleum hydrocarbons weathered (TPH-W); a control treatment with 2 607 mg.kg-1 d. b. was used. Each experiment, with eight replicates per treatment, evaluated after three and six months: a) microbial density of total free-living nitrogen-fixing bacteria (NFB) of Azospirillum (AZP) and Azotobacter group (AZT), for viable count in serial plate; b) dry matter production (DMP), quantified gravimetrically as dry weight of L. hexandra; and c) the decontamination percentage of hydrocarbons (PDH) by Soxhlet extraction. In soil with TPH-F, the NFB, AZP y AZT populations were stimulated five times more than the control both at the three and six months; however, concentrations of 150 000 and 180 000 mg.kg-1 d. b. inhibited the bacterial density between 70 and 89 %. Likewise, in soil with TPH-W, the FNB, AZP and AZT inhibitions were 90 %, with the exception of the 14 173 mg.kg-1 d. b. treatment, which stimulated the NFB and AZT in 2 and 0.10 times more than the control, respectively. The DMP was continued at the six months in the experiments, with values of 63 and 89 g in fresh and weathered petroleum, respectively; had no significant differences with the control (p≤0.05). The PDH reached values of 66 to 87 % both TPH-F and TPH-W at six months, respectively. These results demonstrated the ability the L. hexandra rhizosphere to stimulate the high NFB density, vegetal biomass production and phytoremediation of contaminated soils (with fresh and weathered petroleum), in a tropical waterlogging environment.

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Dual effects of biochar and hyperaccumulator Solanum nigrum L. on the remediation of Cd-contaminated soil

PeerJ ◽

10.7717/peerj.6631 ◽

2019 ◽

Vol 7 ◽

pp. e6631 ◽

Cited By ~ 2

Author(s):

Kang Li ◽

Baoshan Yang ◽

Hui Wang ◽

Xiaohan Xu ◽

Yongchao Gao ◽

...

Keyword(s):

Acid Phosphatase ◽

Contaminated Soil ◽

Contaminated Soils ◽

Dry Weight ◽

Biochemical Properties ◽

Solanum Nigrum ◽

Simultaneous Application ◽

Cd Accumulation ◽

Negative Effect ◽

Solanum Nigrum L

Biochar was widely developed for the soil amendment and remediation of heavy metal contaminated soil. The Cd hyperaccumulator, Solanum nigrum L., has been paid much more attention with the wide application of phytoremediation. The effects of biochar on the growth and accumulation capacity of Solanum nigrum L. in Cd contaminated soil have not been explored so far. The objectives of this study were to explore the dual effects of biochar addition on available Cd in the soil and hyperaccumulation of Cd in Solanum nigrum L. under different Cd contaminated levels. The correlations of soil physicochemical and biochemical properties and Cd absorption of Solanum nigrum L. were analyzed after a 60-day pot experiment under three biochar doses (0%, 1% and 5%) and four Cd concentrations (0, 25, 50 and 100 mg kg−1). The availability of Cd obtained by DTPA extraction significantly decreased after biochar application (P = 0.003, P = 0.0001, P = 0.0001 under 1% biochar addition for 25, 50, and 100 mg kg−1 Cd concentrations, P = 0.0001, P = 0.0001, P = 0.0001 under 5% biochar addition for 25, 50, and 100 mg kg−1 Cd concentrations, n ≥ 3). The 1% biochar dose significantly increased leaf dry weight (P = 0.039, P = 0.002 for the Cd concentrations of 50 and 100 mg kg−1, n ≥ 3) compared with the control in higher Cd concentrations (50 and 100 100 mg kg−1). In the presence of biochar, the bioconcentration factor (BCF) increased under the Cd concentrations of 50 and 100 mg kg−1. The translocation factors (TF) decreased with the biochar doses under the Cd concentration of 100 mg kg−1. The dose of 5% biochar significantly increased the urease activity by 41.18% compared to the 1% biochar addition in the Cd contaminated soil of 50 mg kg−1 concentration. The activities of acid phosphatase were inhibited by 1% biochar dose in all the Cd contaminated soils. The dry weight of the root of Solanum nigrum L. was significantly negatively correlated with acid phosphatase activity and BCF, respectively, indicating acid phosphatase in the rhizosphere soil of Solanum nigrum L. were repressed by Cd toxicity despite of biochar amendment. Biochar had no negative effect on Cd accumulation ability of Solanum nigrum L. Two-way ANOVA analysis showed that both biochar and Cd significantly affected the height of Solanum nigrum L. and the dry weight of leaf and stem. This study implied that biochar addition does not limit the absorption of hyperaccumulator Solanum nigrum L. in the remediation of Cd-contaminated soil. This study implied that the simultaneous application of biochar and hyperccumulator Solanum nigrum L. is promising during the remediation of Cd-contaminated soil.

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Effects of Leachate Fertigation and the Addition of Hydrogen Peroxide on Growth and Nutrient Balance in Dracaena deremensis Potted Plants

Agronomy ◽

10.3390/agronomy11010127 ◽

2021 ◽

Vol 11 (1) ◽

pp. 127

Author(s):

Pedro García-Caparrós ◽

Cristina Velasquez Espino ◽

María Teresa Lao

Keyword(s):

Nutrient Solution ◽

Soluble Sugar ◽

Nutrient Balance ◽

Treatment Plant ◽

Dry Weight ◽

Sugar Concentration ◽

Control Treatment ◽

Peat Moss ◽

Clear Trend ◽

Total Soluble Sugar

The reuse of drainages for cultivating more salt tolerant crops can be a useful tool especially in arid regions, where there are severe problems for crops water management. Dracaena deremensis L. plants were cultured in pots with sphagnum peat-moss and were subjected to three fertigation treatments for 8 weeks: control treatment or standard nutrient solution (D0), raw leachates from Chrysalidocarpus lutescens H. Wendl plants (DL) and the same leachate blending with H2O2 (1.2 M) at 1% (v/v) (DL + H2O2). After harvesting, ornamental and biomass parameters, leaf and root proline and total soluble sugar concentration and nutrient balance were assessed in each fertigation treatment. Plant height, leaf and total dry weight had the highest values in plants fertigated with leachates with H2O2, whereas root length, leaf number, RGB values and pigment concentration declined significantly in plants fertigated with leachates from C. lutescens with or without H2O2. The fertigation with leachates, regardless of the presence or absence of H2O2 increased root and leaf proline concentration. Nevertheless, root and leaf total soluble sugar concentration did not show a clear trend under the treatments assessed. Regarding nutrient balance, the addition of H2O2 in the leachate resulted in an increase in plant nutrient uptake and efficiency compared to the control treatment. The fertigation with leachates with or without H2O2 increased nitrogen and potassium leached per plant compared to plants fertigated with the standard nutrient solution. The reuse of drainages is a viable option to produce ornamental plants reducing the problematic associated with the water consumption and the release of nutrients into the environment.

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Fractionation of Heavy Metals in Multi-Contaminated Soil Treated with Biochar Using the Sequential Extraction Procedure

Biomolecules ◽

10.3390/biom11030448 ◽

2021 ◽

Vol 11 (3) ◽

pp. 448

Author(s):

Mahrous Awad ◽

Zhongzhen Liu ◽

Milan Skalicky ◽

Eldessoky S. Dessoky ◽

Marian Brestic ◽

...

Keyword(s):

Heavy Metals ◽

Organic Matter ◽

Soil Organic Matter ◽

Soil Ph ◽

Contaminated Soil ◽

Contaminated Soils ◽

Extraction Procedure ◽

Sequential Fractionation ◽

Relationship Of ◽

The Relationship

Heavy metals (HMs) toxicity represents a global problem depending on the soil environment’s geochemical forms. Biochar addition safely reduces HMs mobile forms, thus, reducing their toxicity to plants. While several studies have shown that biochar could significantly stabilize HMs in contaminated soils, the study of the relationship of soil properties to potential mechanisms still needs further clarification; hence the importance of assessing a naturally contaminated soil amended, in this case with Paulownia biochar (PB) and Bamboo biochar (BB) to fractionate Pb, Cd, Zn, and Cu using short sequential fractionation plans. The relationship of soil pH and organic matter and its effect on the redistribution of these metals were estimated. The results indicated that the acid-soluble metals decreased while the fraction bound to organic matter increased compared to untreated pots. The increase in the organic matter metal-bound was mostly at the expense of the decrease in the acid extractable and Fe/Mn bound ones. The highest application of PB increased the organically bound fraction of Pb, Cd, Zn, and Cu (62, 61, 34, and 61%, respectively), while the BB increased them (61, 49, 42, and 22%, respectively) over the control. Meanwhile, Fe/Mn oxides bound represents the large portion associated with zinc and copper. Concerning soil organic matter (SOM) and soil pH, as potential tools to reduce the risk of the target metals, a significant positive correlation was observed with acid-soluble extractable metal, while a negative correlation was obtained with organic matter-bound metal. The principal component analysis (PCA) shows that the total variance represents 89.7% for the TCPL-extractable and HMs forms and their relation to pH and SOM, which confirms the positive effect of the pH and SOM under PB and BB treatments on reducing the risk of the studied metals. The mobility and bioavailability of these metals and their geochemical forms widely varied according to pH, soil organic matter, biochar types, and application rates. As an environmentally friendly and economical material, biochar emphasizes its importance as a tool that makes the soil more suitable for safe cultivation in the short term and its long-term sustainability. This study proves that it reduces the mobility of HMs, their environmental risks and contributes to food safety. It also confirms that performing more controlled experiments, such as a pot, is a disciplined and effective way to assess the suitability of different types of biochar as soil modifications to restore HMs contaminated soil via controlling the mobilization of these minerals.

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Dynamic of Morphological and Physiological Parameters and Variation of Soil Characteristics during Miscanthus × giganteus Cultivation in the Diesel-Contaminated Land

Agronomy ◽

10.3390/agronomy11040798 ◽

2021 ◽

Vol 11 (4) ◽

pp. 798

Author(s):

Valentina Pidlisnyuk ◽

Andriy Herts ◽

Volodymyr Khomenchuk ◽

Aigerim Mamirova ◽

Oleksandr Kononchuk ◽

...

Keyword(s):

Contaminated Soil ◽

Contaminated Soils ◽

Soil Characteristics ◽

Physiological Parameters ◽

Wastewater Sludge ◽

Contaminated Land ◽

Miscanthus Giganteus ◽

Uncontaminated Soil ◽

Stems And Leaves ◽

Biochar Amendment

Miscanthus × giganteus (M. × giganteus) is a perspective plant produced on marginal and contaminated lands with biomass used for energy or bioproducts. In the current study, M. × giganteus development was tested in the diesel-contaminated soils (ranged from 250 mg kg−1 to 5000 mg kg−1) and the growth dynamic, leaves quantity, plants total area, number of harvested stems and leaves, SPAD and NPQt parameters were evaluated. Results showed a remarkable M. × giganteus growth in a selected interval of diesel-contaminated soil with sufficient harvested biomass. The amendment of soil by biochar 1 (produced from wastewater sludge) and biochar 2 (produced from a mixture of wood waste and biohumus) improved the crop’s morphological and physiological parameters. Biochar 1 stimulated the increase of the stems’ biomass, while biochar 2 increased the leaves biomass. The plants growing in the uncontaminated soil decreased the content of NO3, pH (KCl), P2O5 and increased the content of NH4. Photosynthesis parameters showed that incorporating biochar 1 and biochar 2 to the diesel-contaminated soil prolonged the plants’ vegetation, which was more potent for biochar 1. M. × giganteus utilization united with biochar amendment can be recommended to remediate diesel-contaminated land in concentration range 250–5000 mg kg−1.

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Synergistic remediation of PCB-contaminated soil with nanoparticulate zero-valent iron and alfalfa: targeted changes in the root metabolite-dependent microbial community

Environmental Science Nano ◽

10.1039/d1en00077b ◽

2021 ◽

Vol 8 (4) ◽

pp. 986-999

Author(s):

Ting Wu ◽

Yangzhi Liu ◽

Kun Yang ◽

Lizhong Zhu ◽

Jason C. White ◽

...

Keyword(s):

Microbial Community ◽

Contaminated Soil ◽

Contaminated Soils ◽

Zero Valent Iron ◽

New Strategy

This work provides a new strategy using nanomaterial-facilitated phytoremediation to promote the restoration of POP-contaminated soils.

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Effect of compost in phytoremediation of diesel-contaminated soils

Water Science & Technology ◽

10.2166/wst.2001.0102 ◽

2001 ◽

Vol 43 (2) ◽

pp. 291-295 ◽

Cited By ~ 26

Author(s):

J. Vouillamoz ◽

M. W. Milke

Keyword(s):

Moisture Content ◽

Contaminated Soil ◽

Contaminated Soils ◽

Petroleum Hydrocarbons ◽

Dilution Effect ◽

Screening Tests ◽

Total Petroleum Hydrocarbons ◽

Controlled Environment ◽

Grass Growth ◽

The Impact

The effect of compost on phytoremediation of diesel-contaminated soils was investigated using 130 small (200 g) containers in two screening tests. The experiments were conducted in a controlled environment using ryegrass from seed. Containers were destructively sampled at various times and analyzed for plant mass and total petroleum hydrocarbons. The results indicate that the presence of diesel reduces grass growth, and that compost helps reduced the impact of diesel on grass growth. The addition of compost helps increase diesel loss from the soils both with and without grass, though the addition of grass leads to lower diesel levels compared with controls. A second set of experiments indicates that the compost helps in phytoremediation of diesel-contaminated soil independent of the dilution effect that compost addition has. The results indicate that the compost addition allowed diesel loss down to 200 mg TPH/kg even though the compost would be expected to hold the diesel more tightly in the soil/compost mixture. The simplicity of the screening tests led to difficulties in controlling moisture content and germination rates. The conclusion of the research is that the tilling of compost into soils combined with grass seeding appears to be a valuable option for treating petroleum-contaminated soils.

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