scholarly journals POLYMORPHISM OF DNA MICROSATELLITE AMONG CHUKOTKA REINDEER

2020 ◽  
pp. 49-53
Author(s):  
Vladimir Vladimirovich Dodokhov ◽  
Nadezhda Ivanovna Pavlova ◽  
Lyubov Aleksandrovna Kalashnikova
Keyword(s):  
Rehabilitation Program ◽  
Dna Test ◽  
Methods Development ◽  
Expected Heterozygosity ◽  
Reindeer Breeding ◽  
Wide Range ◽  
Breeding Condition ◽  
The Republic ◽  
Dna Microsatellite ◽  
Population Program

The article presents the results of DNA microsatellite polymorphism with the use of a commercial kit containing 16 markers: Rt6, BMS1788, Rt30, Rt1, Rt9, C143, Rt7, OheQ, FCB193, C217, Rt24, BMS745, NVHRT16, T40 и C276. The study object is domestic reindeer procured from Chukotka AO to Nizhnekolymsky district of the Republic of Sakha (Yakutia) to refresh the blood of Chukotka reindeer under the rehabilitation program and improvement of reindeer breeding condition. Allele frequency, indicators of observed and expected heterozygosity, fix index and polymorphism were calculated. The calculation of allele frequencies showed that microsatellite markers have a wide range of alleles and high informative value for detecting genetic differences between animals and groups of animals. A total of 116 alleles were identified, which gave an average of 7.25 alleles per locus, and the number of effective alleles was 3.9. Indicators of observed and expected heterozygosity were 0.713 and 0.691. The highest number of alleles 12 and 13 were found in locus OheQ и BMS1788 and the smallest in locus C 143, which had 2 alleles. Also, 3 alleles were identified in locus C217 and C32. The results of the study can contribute to receiving information on the genetic structure of the Chukchi reindeer and using an improvement population program in Yakutia. Moreover, the results can be used in methods development and DNA test rules in the northern reindeer breeding.

The way of value of Correlation of genomic DNA microsatellite loci and live weight of Chukchi reindeer

2020 ◽  
pp. 27-32
Author(s):  
V. Dodokhov ◽  
N. Pavlova ◽  
T. Rumyantseva ◽  
L. Kalashnikova
Keyword(s):  
Microsatellite Markers ◽  
Agricultural Production ◽  
Live Weight ◽  
Biodiversity Loss ◽  
Tribal Community ◽  
High Germination ◽  
Wide Range ◽  
The Mean ◽  
High Level ◽  
Dna Microsatellite

The article presents the genetic characteristic of the Chukchi reindeer breed. The object of the study was of the Chukchi reindeer. In recent years, the number of reindeer of the Chukchi breed has declined sharply. Reduced reindeer numbers could lead to biodiversity loss. The Chukchi breed of deer has good meat qualities, has high germination viability and is adapted in adverse tundra conditions of Yakutia. Herding of the Chukchi breed of deer in Yakutia are engaged only in the Nizhnekolymsky district. There are four generic communities and the largest of which is the agricultural production cooperative of nomadic tribal community «Turvaurgin», which was chosen to assess the genetic processes of breed using microsatellite markers: Rt6, BMS1788, Rt 30, Rt1, Rt9, FCB193, Rt7, BMS745, C 143, Rt24, OheQ, C217, C32, NVHRT16, T40, C276. It was found that microsatellite markers have a wide range of alleles and generally have a high informative value for identifying of genetic differences between animals and groups of animal. The number of identified alleles is one of the indicators of the genetic diversity of the population. The total number of detected alleles was 127. The Chukchi breed of deer is characterized by a high level of heterozygosity, and the random crossing system prevails over inbreeding in the population. On average, there were 7.9 alleles (Na) per locus, and the mean number of effective alleles (Ne) was 4.1. The index of fixation averaged 0.001. The polymorphism index (PIC) ranged from 0.217 to 0.946, with an average of 0.695.

scholarly journals Securitisation gaps: Towards ideational understandings of state weakness

2021 ◽  
pp. 1-20
Author(s):  
Kevork Oskanian
Keyword(s):  
The State ◽  
Collective Knowledge ◽  
State Action ◽  
Role Of The State ◽  
Wide Range ◽  
State And Society ◽  
Interpretive Approach ◽  
Recent Developments ◽  
The Republic

Abstract This article contributes a securitisation-based, interpretive approach to state weakness. The long-dominant positivist approaches to the phenomenon have been extensively criticised for a wide range of deficiencies. Responding to Lemay-Hébert's suggestion of a ‘Durkheimian’, ideational-interpretive approach as a possible alternative, I base my conceptualisation on Migdal's view of state weakness as emerging from a ‘state-in-society's’ contested ‘strategies of survival’. I argue that several recent developments in Securitisation Theory enable it to capture this contested ‘collective knowledge’ on the state: a move away from state-centrism, the development of a contextualised ‘sociological’ version, linkages made between securitisation and legitimacy, and the acknowledgment of ‘securitisations’ as a contested Bourdieusian field. I introduce the concept of ‘securitisation gaps’ – divergences in the security discourses and practices of state and society – as a concept aimed at capturing this contested role of the state, operationalised along two logics (reactive/substitutive) – depending on whether they emerge from securitisations of the state action or inaction – and three intensities (latent, manifest, and violent), depending on the extent to which they involve challenges to state authority. The approach is briefly illustrated through the changing securitisation gaps in the Republic of Lebanon during the 2019–20 ‘October Uprising’.

ORGANIZATION OF ROAD TRAFFIC TAKING INTO ACCOUNT ELECTRIC ROUTE PASSENGER TRANSPORT

2021 ◽  
Vol 2 (65) ◽  
pp. 66-77
Author(s):  
Denis V. Kapsky ◽  
◽  
Sergey S. Semchenkov ◽  
Evgeny N. Kot ◽  
◽  
...  
Keyword(s):  
Traffic Management ◽  
Road Traffic ◽  
Rapid Development ◽  
Living Environment ◽  
Transport Sector ◽  
Passenger Transport ◽  
Wide Range ◽  
Practical Development ◽  
The Republic ◽  
Conducting Research

The rapid development of the transport sector leads not only to positive changes in the life of cities and towns, to an increase in convenience and comfort for residents, but also worsens the ecology and their living environment. The “profitable-safe” dilemma can be solved by the approach of environmentally oriented selection of the type of route passenger transport and decisionmaking in favor of electric route passenger transport. The article discusses the types of such transport on the example of the experience of their use in the Republic of Belarus. The classification and systematization developed by the authors are presented with subsequent recommendations for its application. On the basis of the results of the authors’ research, the issues of interaction of rail and non-rail route vehicles with an electric drive with the organization of road traffic are separately considered. The presented materials can be useful to a wide range of readers, as well as to specialists conducting research and practical development in the field of electrically powered route vehicles and traffic management.

On the work of the Scientific Medical Society of Surgeons of the Republic of Tatarstan in 1996

1997 ◽  
Vol 78 (2) ◽  
pp. 158-159
Author(s):  
I. A. Ibatullin ◽  
A. Yu. Anisimov
Keyword(s):  
Public Health ◽  
Health Care ◽  
Practical Experience ◽  
Public Health Care ◽  
Medical Society ◽  
Clinical Observations ◽  
Medical Institutions ◽  
Wide Range ◽  
The Republic

The activity of the oldest scientific medical society of surgeons in Tatarstan is especially relevant in the conditions of the period of profound reforming of the society and the system of public health care that we are going through. Its work is a reliable means of transferring up-to-date information and advanced practical experience from some leading clinics and departments to the widest circle of physicians and teams of medical institutions of surgical profile. At the meetings of NMOH RT the most interesting issues in different branches of surgery and adjacent spheres: anesthesiology, gynecology and oncology were discussed. A wide range of possibilities of Kazan Surgical School was presented in the form of demonstrations of clinical observations and educational and methodological videos.

scholarly journals Population structure and phylogenetic relationships in Brassica rapa L. subspecies by using isozyme markers

2020 ◽  
Author(s):  
R. H. Sammour ◽  
M. A. Karam ◽  
Y. S. Morsi ◽  
R. M. Ali
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Brassica Rapa ◽  
Phylogenetic Relationships ◽  
Fixation Index ◽  
Distribution Area ◽  
Enzyme Analysis ◽  
Breeding Programs ◽  
Expected Heterozygosity ◽  
Wide Range

Abstract The present study aimed to assess population structure and phylogenetic relationships of nine subspecies of Brassica rapa L. represented with thirty-five accessions cover a wide range of species distribution area using isozyme analysis in order to select more diverse accessions as supplementary resources that can be utilized for improvement of B. napus. Enzyme analysis resulted in detecting 14 putative polymorphic loci with 27 alleles. Mean allele frequency 0.04 (rare alleles) was observed in Cat4A and Cat4B in sub species Oleifera accession CR 2204/79 and in subspecies trilocularis accessions CR 2215/88 and CR 2244/88. The highest genetic diversity measures were observed in subspecies dichotoma, accession CR 1585/96 (the highest average of observed (H0) and expected heterozygosity (He), and number of alleles per locus (Ae)). These observations make this accession valuable genetic resource to be included in breeding programs for the improvement of oilseed B. napus. The average fixation index (F) is significantly higher than zero for the analysis accessions indicating a significant deficiency of heteozygosity. The divergence among subspecies indicated very great genetic differentiation (FST = 0.8972) which means that about 90% of genetic diversity is distributed among subspecies, while 10% of the diversity is distributed within subspecies. This coincides with low value of gene flow (Nm = 0.0287). B. rapa ssp. oleifera (turnip rape) and B. rapa ssp. trilocularis (sarson) were grouped under one cluster which coincides with the morphological classification.

scholarly journals Serbian theolinguistics today: Research issues and results

2016 ◽  
Vol 72 (1-2) ◽  
pp. 159-186
Author(s):  
Ksenija Koncarevic ◽  
Srdjan Petrovic
Keyword(s):  
Basic Research ◽  
Discourse Theory ◽  
Scientific Journals ◽  
Research Directions ◽  
Research Issues ◽  
Methodological Basis ◽  
Wide Range ◽  
The Republic ◽  
General Linguistics ◽  
Slavic Studies

The essay gives a survey of basic research directions in Serbian theolinguistics (in the fields of general linguistics, Serbian studies, Slavic studies and foreign philology), and presents the most important achievements in the fundamental and applicative fields of the study of the sacral language (from the synchronic perspective) which are presented in monographs, papers published in thematic anthologies, proceedings of scientific conferences and scientific journals in Serbia, Montenegro and the Republic of Srpska (with bibliographic references for the 2000-2013 period). Serbian theolinguistics, although in the stage of scientific constitution, potentially has a wide range of fundamental areas of study (some of the main issues from 2000 to 2013 were theoretical and methodological basis of theolinguistics, modern functioning of liturgical languages, confessional markedness of language levels, functional stylistics, genology and stylistics of resources, discourse theory) and spheres of application (lexicography, traductology, linguodidactics). Its perspective in the forthcoming period lies in strengthening the ties with leading centres of theolinguistics in the Slavic world and the integration of researchers of philological and theological profiles in order to further its development.

scholarly journals Plato's “Republic”: an example of proto-project mental activity and primary experience in design of new social forms

2021 ◽  
pp. 58-66
Author(s):  
Polina Rybalkina
Keyword(s):  
Cultural Practices ◽  
Mental Activity ◽  
Significant Part ◽  
Project Approach ◽  
Project Activity ◽  
Social Forms ◽  
Wide Range ◽  
The Republic ◽  
Personal Fulfillment

This article is dedicated to the analysis of Plato's work “The Republic” in the context of the genesis of project mentality  and project activity as the anthropological outcome that goes back to the early cultural states and primary personality-oriented ancient practices. The author shifts to the plane of historical-cultural retrospective of the origin of the phenomenon of project mentality with the objective vicissitudes of its genesis. An attempt is made to reflect on this process, examine and clarify certain moments associated with the prelude and dynamics of the development of project approach towards organizing modern reality. The author substantiates the original perspective on interpretation of Plato's “Republic” as the early typical example of proto-project mentality formed in the depths of ancient cultural practices. Project mentality is a promising vector of the existence of culture, the so-called matrix of personal fulfillment of a significant part of our contemporaries. The author believes that Plato's work “The Republic” should be recognized as one of the very first examples of proto-project due to the presence of a wide range of typological attributes of design. The conclusion is made that in accordance to most parameters, Plato's work “The Republic” falls under the concept of “project activity”, as it features majority of the aspects of sociocultural design.

scholarly journals Methods development for diffraction and spectroscopy studies of metalloenzymes at X-ray free-electron lasers

2014 ◽  
Vol 369 (1647) ◽  
pp. 20130590 ◽  
Author(s):  
Jan Kern ◽  
Johan Hattne ◽  
Rosalie Tran ◽  
Roberto Alonso-Mori ◽  
Hartawan Laksmono ◽  
...  
Keyword(s):  
Free Electron ◽  
Water Oxidation ◽  
Spectroscopic Studies ◽  
Free Electron Lasers ◽  
Software Suite ◽  
X Ray ◽  
Methods Development ◽  
Wide Range ◽  
Linac Coherent Light Source ◽  
Spectroscopy Studies

X-ray free-electron lasers (XFELs) open up new possibilities for X-ray crystallographic and spectroscopic studies of radiation-sensitive biological samples under close to physiological conditions. To facilitate these new X-ray sources, tailored experimental methods and data-processing protocols have to be developed. The highly radiation-sensitive photosystem II (PSII) protein complex is a prime target for XFEL experiments aiming to study the mechanism of light-induced water oxidation taking place at a Mn cluster in this complex. We developed a set of tools for the study of PSII at XFELs, including a new liquid jet based on electrofocusing, an energy dispersive von Hamos X-ray emission spectrometer for the hard X-ray range and a high-throughput soft X-ray spectrometer based on a reflection zone plate. While our immediate focus is on PSII, the methods we describe here are applicable to a wide range of metalloenzymes. These experimental developments were complemented by a new software suite, cctbx.xfel . This software suite allows for near-real-time monitoring of the experimental parameters and detector signals and the detailed analysis of the diffraction and spectroscopy data collected by us at the Linac Coherent Light Source, taking into account the specific characteristics of data measured at an XFEL.

Cases of Q fever detected in residents of the novosibirsk region hospitalized with suspection of infections transmitted by ticks

2021 ◽  
Vol 66 (4) ◽  
pp. 229-236
Author(s):  
E. I. Bondarenko ◽  
E. S. Filimonova ◽  
E. I. Krasnova ◽  
E. V. Krinitsina ◽  
S. E. Tkachev
Keyword(s):  
Causative Agent ◽  
Q Fever ◽  
Clinical Manifestations ◽  
Test Methods ◽  
Pcr Analysis ◽  
Laboratory Diagnostics ◽  
Novosibirsk Region ◽  
Complex Approach ◽  
Wide Range ◽  
The Republic

Coxiella burnetii is the causative agent of Q fever (coxiellosis), which, in addition to acute manifestations, often occurs in a latent form, is prone to chronic course and, in the absence of antibiotic therapy, has a high risk of disability or death. As a result of the presence of a wide range of clinical manifestations specific to other infectious diseases, the use of laboratory test methods (LTM) is required to make a diagnosis. The presence of Q fever anthropurgic foci in the Novosibirsk region was described in the 90s of the last century, but due attention to its laboratory diagnostics is not paid in this region. The aim of the study was to identify genetic and serological markers of the causative agent, C. burnetii, in patients of the Novosibirsk region who were admitted for treatment with fever with suspected tick-borne infections (TBIs). DNA marker of the causative agent of Q fever was detected in blood samples by real time PCR in 9 out of 325 patients. In three patients, the presence of C. burnetii DNA was confirmed by sequencing of the IS1111 and htpB gene fragments. In ELISA tests, antibodies against the causative agent of coxiellosis were detected in the blood sera of 4 patients with positive results of PCR analysis. Contact with tick was registered in 7 out of 9 patients who had C. burnetii DNA and lacked markers of other TBIs. Six people were infected in the Novosibirsk region, two suffered from tick’s bite in Altai, and one case was from the Republic of Kyrgyzstan. Thus, a complex approach using both PCR analysis and ELISA provided the identification of markers of the Q fever causative agent in patients admitted with suspected TBIs, thereby differentiating it from other infections. Contact with ticks in most cases suggests that infection with C. burnetii had a transmissible pathway.

LOYALTY ASSESSMENT OF ELDERLY CUSTOMERS TO THE PHARMACY USING KANO MODEL

2021 ◽  
Vol 92 (2) ◽  
pp. 24-33
Author(s):  
S. G. Troina ◽  
◽  
V. V. Kuhach ◽  
Keyword(s):  
Sociological Study ◽  
Kano Model ◽  
Pricing Policy ◽  
One Dimensional ◽  
Wide Range ◽  
Presence And Absence ◽  
The Republic ◽  
Modern Technologies

The article presents the results of a sociological study conducted with the questionnaire of 385 pharmacy customers from different regions of the Republic of Belarus aged over 60 using Kano model. The respondents consistently expressed their attitude to the presence and absence of 13 characteristics (parameters) effecting the performance of pharmacies. The purpose of this study is to assess the loyalty of elderly customers to the pharmacy using Kano model. The potentials of elderly customers’ satisfaction and dissatisfaction with the work of pharmacies are calculated. The same parameter was revealed to have a different effect on the potential of satisfaction and dissatisfaction of pharmacy elderly customers. The distribution of the parameters studied into 4 quadrants allowed to determine mandatory characteristics of the pharmacy (availability of a wide range of products, competence of pharmaceutical workers; provision of the customers with detailed information about the purchased product), linear (location of the pharmacy, convenient schedule of its work, rational pricing policy, discount availability, informative and esthetic design of shop windows, benevolence of pharmaceutical workers) and indifferent ones (availability of car parking near the pharmacy, advertising items in the pharmacy, ability to get additional services in the pharmacy, modern technologies for population service). Attractive parameters were not set. The design of shop windows is at the border edge of mandatory and one-dimensional characteristics intersection. To attract customers and increase their loyalty to the pharmacy it is necessary to ensure availability of mandatory and to develop the category of linear characteristics.

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