scholarly journals Auxin and carotene biosynthesis by the bacterium Pantoea agglomerans

2020 ◽  
Author(s):  
E. A. Gilvanova ◽  
P. Yu. Milman
Keyword(s):  
Amino Acid ◽  
Population Density ◽  
Nutrient Medium ◽  
Maximum Yield ◽  
Pantoea Agglomerans ◽  
Qualitative Composition ◽  
Peptide Component

Monitoring of auxin and carotene during cultivation of the Pantoea agglomerans strain IB-BF revealed that the maximum yield of the target products is provided not by population density, but by the qualitative composition of the nutrient medium and the need for a larger peptide component of the substrate (rich amino acid set), which is part of the standard LB medium.

scholarly journals Production characteristics of the diatom Cylindrotheca closterium (Ehrenb.) Reimann et Lewin grown in an intensive culture at various nitrogen sources in the medium

2019 ◽  
Vol 4 (1) ◽  
pp. 33-44 ◽  
Author(s):  
S. N. Zheleznova
Keyword(s):  
Stationary Phase ◽  
Nitrogen Source ◽  
Nutrient Medium ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Dry Weight ◽  
Sole Source ◽  
Active Growth ◽  
Cylindrotheca Closterium ◽  
Production Characteristics

The diatom Cylindrotheca closterium (Ehrenberg) Reimann et Levin is characterized by high productivity (up to 1.5 g·l-1·day-1) and the ability to accumulate a valuable carotenoid fucoxanthin (up to 2 % of dry weight). In the development of biotechnology based on microalgae, the key issue is the creation of concentrated nutrient medium. Nitrogen is one of the most important components in the nutrient medium that significantly affects the production characteristics of all microalgae. The aim of this study is to compare the production characteristics of C. closterium in an intensive storage culture using different forms of nitrogen in the medium. In the first experiment, nitrate and sodium nitrite, urea, and nitrogen in the form of ammonium were used as a source of nitrogen. The amount of nitrates, nitrites, ammonium, and urea in the medium was calculated from the nitrogen content of the RS nutrient medium, with a nitrogen to phosphorus ratio of 15 : 1. In the second experiment, amino acids were used as a nitrogen source – arginine, asparagine, cysteine. The possibility of using the microalgae C. closterium for the growth of various organic sources of nitrogen (urea, cysteine, asparagine) was shown. Productive characteristics in the intensive storage culture of C. closterium using urea, cysteine, and asparagine as the sole source of nitrogen in the RS nutrient medium were determined. It is shown that when urea was used, the productivity reached its maximum values and amounted to 1.5 g·l-1·day-1. Thus, the expediency of using urea in the medium for obtaining the maximum yield of biomass was shown. The use of cysteine in the stationary phase of growth to achieve a long stationary phase with minimal concentrations of the nitrogen source in the nutrient medium is also advisable. It was found that C. closterium was able to grow and vegetate at sufficiently high concentrations of nitrite, and the addition of nitrogen in ammonium form to the nutrient medium during the active growth of C. closterium led to inhibition of all metabolic processes and to the death of the culture.

Effect of a Leucine Analogue on Incorporation of Glycine into the Proteins of Explanted Chick Embryos

Development ◽  
1958 ◽  
Vol 6 (2) ◽  
pp. 262-269
Author(s):  
Phyllis W. Schultz ◽  
Heinz Herrmann
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Chick Embryo ◽  
Total Protein ◽  
Nutrient Medium ◽  
Chick Embryos ◽  
Agar Gel ◽  
Egg Extract ◽  
Amino Acid Analogues

Amino acid analogues have been observed to give rise to abnormal forms of development of chick and amphibian embryos (Herrmann, 1953; Rothfels, 1954; Waddington & Sirlin, 1954; Feldman & Waddington, 1955; Herrmann, Rothfels-Konigsberg, & Curry, 1955). Assuming that these disturbances may be due to interference with the utilization of amino acids for protein formation, we have attempted an analysis of this effect by comparison of the protein contents and of the uptake of glycine into the proteins of chick embryo explants in the presence and absence of amino acid analogues. The results of such experiments are reported in this paper. The chick embryos used for explanation, the explantation technique, and the determination of total protein glycine and of tracer glycine were essentially the same as described previously (Herrmann & Schultz, 1958). The embryos were explanted at the 11–13 somite stage on to the surface of an agar gel containing egg extract as nutrient medium following the procedure given by Spratt (1947) as modified by Rothfels (1954).

scholarly journals Transfer of serine into polypeptides and myosin by chromatographic species of seryl-transfer ribonucleic acid

1975 ◽  
Vol 146 (2) ◽  
pp. 395-400 ◽  
Author(s):  
M Nwagwu
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Total Protein ◽  
Ribonucleic Acid ◽  
Free Amino ◽  
Maximum Yield ◽  
Amino Acid Incorporation ◽  
Acid Incorporation

The efficiencies of two chromatographic species of [3-H]seryl-tRNA, namely peaks I and II, in cell-free amino acid incorporation were investigated. The maximum yield of polypeptide seems to be the same for the reaction mixtures containing either peak I or peak II, suggesting that the efficiency of both peaks in total protein synthesis is the same. The efficiency of transfer of serine into myosin heavy subunit (myosin H) by peaks I and II was also investigated. Peak II of [3-H]seryl-tRNA transfers three times as much serine into myosin H as peak I.

scholarly journals Bemisia tabaci MED Population Density as Affected by Rootstock-Modified Leaf Anatomy and Amino Acid Profiles in Hydroponically Grown Tomato

2018 ◽  
Vol 9 ◽  
Author(s):  
Katja Žanić ◽  
Gvozden Dumičić ◽  
Marija Mandušić ◽  
Gabriela Vuletin Selak ◽  
Ivana Bočina ◽  
...  
Keyword(s):  
Amino Acid ◽  
Population Density ◽  
Bemisia Tabaci ◽  
Leaf Anatomy ◽  
Amino Acid Profiles ◽  
Hydroponically Grown

scholarly journals Genetic and management adaptation of field bean (Vicia faba L.) in Finland

1981 ◽  
Vol 53 (5) ◽  
pp. 328-340
Author(s):  
Seppo Pulli ◽  
Mauritz Vestberg
Keyword(s):  
Population Density ◽  
Maximum Yield ◽  
Grain Filling ◽  
Climatic Conditions ◽  
Plant Diseases ◽  
Field Bean ◽  
Early Variety ◽  
Seeding Time ◽  
Grain Filling Period ◽  
The University

The investigation of field bean adaptation in Finnish climatic conditions was carried out at the University of Helsinki in 1976—77. The main objectives were to study the effects of seeding time and population density on the quantity and quality of the yield and the vegetative features in the development of two different types of field bean varieties. Field bean yielded 4061 kg/ha in 1976. In 1977only 2042kg/ha was harvested due to the lack of light during the grain filling period and the presense of plant diseases. Delayed seeding lowered yields in both years. Maximum yield was obtained with the seed rate of 240 kg/ha. Two weeks delay in the seeding speeded up flowering by two days. Temperature sum in degree days from seeding to emergence was 140—170°C, from seeding to flowering 618—637°C and from seeding to maturity 1670—1890°C. LAI was 5.7 for early variety and 4.3 for late variety at the time of pod setting representing very effective situation for CGR. Number and distribution of internodes, pods and seeds were primarily influenced by population density and secondly by the differences between varieties.

scholarly journals A comparative study of application of multifunctional fertilizers based on amino acids and trace elements for winter wheat

2020 ◽  
Vol 17 ◽  
pp. 00251
Author(s):  
Olga Shapowal ◽  
Irina Mozharova
Keyword(s):  
Amino Acids ◽  
Trace Elements ◽  
Amino Acid ◽  
Winter Wheat ◽  
Comparative Study ◽  
Grain Yield ◽  
Maximum Yield ◽  
High Yield ◽  
Yield Increase ◽  
Nizhny Novgorod Region

The study of different complexes of new and innovative forms of fertilizers with the same ratio of trace elements applied in Nizhny Novgorod region in 2018 showed that the greatest grain yield increase to 0.45 and 0.435 t/ha or 7.8 and 7.4 % was obtained using trace elements and amino acids in doses of 1.5 and 3.0 l/ha. Similar results were observed when using amino acids in a dose of 2.0 l/ha. The yield Increase was 0.44 t/ha compared to the control one of 2.59 t/ha. In Ryazan region, the maximum yield was obtained using amino acids in a dose 1.0 l/ha; the yield increase was 1.4 t/ha or 28.6 %, while the control yield was 4.9 t/ha. Amino acid with microelements in a dose of 1.5 and 3.0 l/ha gave the high yield; the yield increase was 1 t/ha (20.4 %).

Dental Enamel Matrix — Isolation and Partial Sequence of a Peptide Component

1979 ◽  
Vol 58 (2_suppl) ◽  
pp. 997-999 ◽  
Author(s):  
A.G. Fincham
Keyword(s):  
Amino Acid ◽  
Cyanogen Bromide ◽  
Dental Enamel ◽  
Disc Electrophoresis ◽  
Matrix Proteins ◽  
Partial Sequence ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
Peptide Component ◽  
Electrophoretic Component

Chromatography of demineralized bovine fetal enamel matrix proteins on 'Biogel P6' was shown to yield a trailing peak which contained a single electrophoretic component. This polypeptide, further purified by chromatography on Biogel P4', was found to be homogeneous by disc electrophoresis and gel isoelectric focussing. Amino acid analyses indicated this component (designated: 'E5') to be similar to some of the phosphopeptides previously described. Cyanogen bromide cleavage of E5 yielded three principal products whose amino acid compositions and N-terminal residues were investigated. A partial sequence for component E5 was proposed and comparison with previous bovine matrix isolates was made.

Amino acid composition of azolla as affected by strains and population density

1989 ◽  
Vol 117 (2) ◽  
pp. 263-267 ◽  
Author(s):  
N. Sanginga ◽  
C. Van Hove
Keyword(s):  
Amino Acid ◽  
Population Density ◽  
Amino Acid Composition ◽  
Acid Composition

scholarly journals ПОРІВНЯЛЬНЕ ВИВЧЕННЯ АМІНОКИСЛОТНОГО СКЛАДУ СУХИХ ПОРОШКІВ БІОМАСИ FLAMMULINA VELUTIPES, ЯКІ ОТРИМАНІ МЕТОДАМИ ПОВЕРХНЕВОГО ТА ГЛИБИННОГО КУЛЬТИВУВАННЯ

2015 ◽  
Author(s):  
Т. А. Буткевич ◽  
В. П. Попович
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Submerged Culture ◽  
Microbiological Synthesis ◽  
Qualitative Composition ◽  
Medicinal Mushroom ◽  
Dry Powders ◽  
Quantitative Content ◽  
Static Conditions

<p class="Default"><strong>Summary: </strong> qualitative composition and quantitative content of 17 amino acids, including 7 essential were defined in <em>Flammulina</em><em> velutipes</em> medicinal mushroom’s biomass dry powders. Biomass powders were received in static conditions and by submerged culture.</p><p class="Default"><strong>Key words:</strong> biomass, <em>Flammulina velutipes</em>, cultivation in static conditions, submerged culture, aminoacids, liquid chromatography.</p><p class="Default"><strong> </strong></p><p><strong>Introduction</strong><strong>. </strong>The medicinal mushroom <em>Flammulina (F.) velutipes</em> is widely used in non-traditional and officinal Eastern medicine as immunomodulatory, antitumor, antioxidant, antihypertensive, antibacterial, antifungal, antiviral agent [5; 6].</p><p>Polysaccharide content of <em>F. velutipes</em> biomass was identified in previous studies [7]. Continuing work on the study of bioactive substances of medicinal mushroom we conducted determination of biomass dry powders amino acid composition.</p><p>Amino acids are widely used in medicine for the treatment of neuropsychiatric disorders, some diseases of the gastrointestinal tract and liver, for the prevention of atherosclerosis, to increase immunity and to inhibit tumor development, etc. [1 - 4].</p><p>Thus, the purpose of this work was to determine and compare amino acids qualitative composition and quantitative content in <em>F. velutipes</em> biomass dry powders.</p><p><strong>Materials and methods</strong><strong></strong></p><p>The objects of our study were two types of medicinal mushroom <em>F. velutipes</em> biomass dry powders. They were obtained by two methods of microbiological synthesis – cultivation in static conditions and by submerged culture.</p><p>Water suspension with waste of CO<sub>2</sub>-extraction – Amaranthus flour (60 g/l) as a submerged culture medium for <em>F. velutipes</em> biomass production was explored. Mycelia were grown in 500 ml of microbial mattress at 26 ±1°C and pH value 6,0.  Biomass was dried in a vacuum freeze dryer Cryodos-500 (Terrasa,Spain).</p><p>Amino acid composition was studied on an automatic amino acid analyzer T-339 («Mikrotechna», Prague, Czech Republic) by ion-exchange liquid chromatography at the laboratory of the Palladin Institute of biochemistry of the National Academy of Sciences of Ukraine.</p><p><strong>Results and discussion</strong></p><p>17 amino acids, including 7 essential (valine, isoleucine, leucine, lysine, methionine, threonine, phenylalanine), 2 conditionally essential (arginine and histidine) and 8 replaceable amino acids (alanine, aspartic acid, glycine, glutamic acid, proline, serine, tyrosine, cystine) were identified in <em>F. velutipes</em> biomass dry powders. Biomass dry powders obtained by different methods of microbiological synthesis (cultivation in static conditions and by  submerged culture) do not differ in amino acids qualitative composition.</p><p>However, the biomass dry powder of medicinal mushroom that was obtained in static conditions contains 1.41 times more amino acids (essential to 1.27 times more; replaceable – 1.46 times more).</p><p><strong>Conclusions</strong></p><p>1. Using ion-exchange liquid chromatography in <em>F. velutipes</em> biomass dry powders were identified 17 amino acids, including 7 essential, 2 conditionally essential and 8 replaceable ones. Biomass dry powders were obtained by cultivation in static conditions and by submerged culture. The dominant of the essential amino acids in both investigated powders are leucine, of replaceable ones – glutamic and aspartic acid.</p>2. Cultivation of medicinal mushroom <em>F. velutipes</em> in static conditions allows to obtain in 1.41 times more amino acids compared to the submerged culture.

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