scholarly journals Isolasi Dan Karakterisasi Bakteri Selulolitik Biopori Sebagai Upaya Awal Percepatan Proses Pengomposan

2020 ◽  
Vol 4 (1) ◽  
pp. 48-54
Author(s):  
Wardha Maulidya Pratiwi ◽  
Rana Yumna Nabila ◽  
Heliza Amalini ◽  
Guntur Trimulyono
Keyword(s):  
Cellulose Degradation ◽  
Methyl Cellulose ◽  
Test Results ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Bacterial Isolation ◽  
Plate Method ◽  
Yellow Pigmentation ◽  
Ferment Lactose

The purpose of this study was to obtain cellulolytic bacterial isolates isolated from leaf litter in absorption holes biopori FMIPA Unesa and obtain the most optimal cellulolytic bacterial isolates in cellulose degradation. This research was an observational study and the data were analyzed descriptively. Stages of the study began with bacterial isolation, cellulolytic ability testing, and characterization of cellulolytic bacterial isolates. Bacterial isolation was carried out by the pour plate method, isolation was carried out by the streak plate method, cellulolytic testing was carried out using Carboxy Methyl Cellulose media which was given Congo red 1%. While the isolation characterization was done morphologically, physiologically, and biochemically. The results obtained 15 isolates of cellulolytic bacteria that were tested for their ability to degrade cellulose. Cellulolytic test results showed that 6 isolates, namely BS1, BS7, BS10, BS11, BS14, and BS15 had a cellulolytic index of 0.8, 0.8, 0.8, 0.8, 0.8, 0.8. and 1. Isolates BS15 is the most optimal isolate in cellulose degradation with characteristics of the punctiform colony, yellow pigmentation, entire edge, convex elevation, optical opaque and smooth surface, produces catalase enzymes, is non-motile and can ferment in glucose and starch but cannot ferment lactose and also a Gram-negative

scholarly journals ISOLASI DAN UJI KOMPATIBILITAS BAKTERI HIDROLITIK DARI TANAH TEMPAT PEMROSESAN AKHIR TALANGAGUNG, KABUPATEN MALANG

2020 ◽  
Vol 19 (2) ◽  
Author(s):  
Prilya Dewi Fitriasari ◽  
Nanda Amalia ◽  
Susiyanti Farkhiyah
Keyword(s):  
Proteolytic Activity ◽  
Organic Waste ◽  
Skim Milk ◽  
Enzymatic Activities ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Bacterial Isolation ◽  
Plate Method ◽  
Selective Media ◽  
Bacterial Selection

Talangagung landfill, Malang Regency has used the controlled landfill method. The landfill process produces methane gas that has been flowed as fuel so that it is estimated that there are bacteria with a variety of enzymatic activities that have worked in degrading waste. The purpose of this study was to isolate bacteria from landfill and determine the ability to produce amylase, protease, lipase, and cellulase and test the compatibility of bacteria. The methods used include bacterial isolation using the pour plate method and purification of bacterial isolates using the streak plate method. Bacterial selection is done on selective media containing starch, tributyrin, cellulose, and skim milk. There were 21 bacterial isolates successfully isolated 13 consisting of bacterial isolates showed proteolytic activity, 10 cellulolytic bacteria, 8 amylolytic, and 15 lipolytic. The isolates that show compatible results are BTA 5.7.14, BTA 5.7.7, BTA 5.7.12, and BTA 5.7.6 therefore they can be used as a potential consortium for organic waste recycle.   

scholarly journals Biochemical Characterization of Cellulolitic Bacteria From Mangroves Weathered Wood In Muntok Sub district, West Bangka Regency

2018 ◽  
pp. 49
Author(s):  
Ardiansyah Kurniawan ◽  
Asep Awaludin Prihanto ◽  
Suci Puspita Sari ◽  
Andi Kurniawan ◽  
Euis Asriani ◽  
...  
Keyword(s):  
Environmental Changes ◽  
Biochemical Characterization ◽  
Animal Feed ◽  
Cellulose Degradation ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Human Needs ◽  
Sampling Locations

Acceleration of wood weathering in the mangrove area is possible with the role of cellulolytic bacteria. Cellulolytic bacteria producing cellulase enzymes are needed by the animal feed and agriculture industries. Mangroves in Muntok Subdistrict undergo a process of adaptation to environmental changes due to tin mining, including microorganisms. Exploration of new species and strains of cellulolytic bacteria in miningaffected mangroves is needed to enrich the collection and its potential use for human needs. This study aims to identify cellulolytic bacteria in mangrove weathered wood in Muntok District, West Bangka Regency through isolation, screening, and biochemical characterization. Three sampling locations, namely Sukal Mangrove, Peltim Mangrove, and Tembelok Mangrove result 22 bacterial isolates with 11 isolates showing the ability of cellulose degradation in qualitative tests using Lugol. The biochemical characterization of bacterial isolates with the greatest cellulose degradation was directed towards Citrobacter freundii and Vibrio alginolyticus in samples of Sukal Mangrove and Actinomyces bovis in isolates from Peltim Mangrove.

scholarly journals The Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
Siti Khotimah ◽  
Suharjono Suharjono ◽  
Tri Ardyati ◽  
Yulia Nuraini
Keyword(s):  
Organic Matter ◽  
Oil Palm ◽  
Secondary Forest ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Isolation And Identification ◽  
Plate Method ◽  
Cellulase Enzyme ◽  
Degrading Bacteria ◽  
Relationship Of

Abstract. Khotimah S, Suharjono, Ardyati T, Nurani Y. 2020. Isolation and identification of cellulolytic bacteria at fibric, hemic, and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia. Biodiversitas 21: 2103-2112. Cellulose degrading bacteria was one of the microbial removers of organic matter contained in the soil into simpler monomers so that it can be utilized by other organisms. The objective of the research was to obtain cellulose-degrading bacteria found on fibric, hemic, and sapric peat in forest and shrubs (oil palm). The bacteria were isolated by pour plate method on 1% CMC media. Selected isolates were assayed quantitatively based on the activity of cellulase enzyme, identified with 16S rDNA. The density of cellulolytic bacteria in the secondary forest peat of fibric, hemic, sapric were 2.1x103 cfu/g, 5.9x104 cfu/g, and 4.9x104 cfu/g whereas, in the area of shrubs/oil palm peat fibric, hemic and sapric 6.9x104 cfu/g, 8.4x104 cfu/g and 3.4x105 cfu/g respectively. There were 19 bacterial isolates that have clear zones around the colony as degradation of cellulose had highest ability to degrade cellulose with clear zones of 5-7 mm. The strain of SB1.1.1 showed highest activity of cellulase enzyme 11.17 U/mL, followed by HH3.1.1 strain and SB2.3 7.83 U/mL. Based on the phylogeny tree, strain SB1.1.1 and HH3.1.1 have the closest kinship relationship with Bacillus cereus with a kinship relationship of 100%, while SB2.3 has the closest kinship relationship with Bacillus stratosphericus with a relationship of 99.85 %.

scholarly journals PEMBUATAN DAN KARAKTERISASI METIL SELULOSA DARI BONGGOL DAN KULIT NANAS (Ananas comosus)

Alotrop ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Lena Rahmidar ◽  
Seruni Wahidiniawati ◽  
Tety Sudiarti
Keyword(s):  
Methylene Chloride ◽  
Carbon Sources ◽  
Methyl Cellulose ◽  
Wave Number ◽  
Ananas Comosus ◽  
Test Results ◽  
Microbial Cellulose ◽  
Second Stage ◽  
The Core

[MAKING AND CHARACTERIZATION OF CELLULOSE METHOD OF BONGGOL AND LEATHER LEATHER (Ananas comosus)] Wastes of core and  peel of pineapple are often not utilized properly, in fact the waste has nutritional content that can be useful. Carbohydrate content in the waste is large enough and allows to be utilized. The purpose of this research is for converting glucose into cellulose and synthesized into methylcellulose. The first stage is glucose from core and peel of pineapple converted into microbial cellulose with Acetobacter xylinum bacteria with the addition of nitrogen and carbon sources. The second stage is synthesis of methylcellulose with methylene chloride and the solvent aquadest/acetone. The dried microbial cellulose was firstly swelled with NaOH addition, then methylation by addition of solvent and methylene chloride and then refluxed at 50-60. Then methyl cellulose neutralized, washed and dried at 50.The FTIR results showed typical cellulose uptake for cellulose microbial pineapple core at wave number 3377.36 cm-1 for OH bonds and 2935.66 cm-1 for CH bonds and in cellulose microbialpineapple peel at wave number 3377.36 cm-1 for OH bonds and 2935.66 cm-1 for the CH bonds. The ratio OH/CH of methyl cellulose-acetone has a smaller value in the core and peel of pineapple concluded that acetone is more efficient to use as a solvent at the methylation. The substitution value of the core and peel of pineapple skin is estimated to below based on the solubility test results.

A Modified Microtiter Plate Method for Biochemical Characterization of Listeria spp.

1991 ◽  
Vol 54 (2) ◽  
pp. 121-123 ◽  
Author(s):  
GREGORY R. SIRAGUSA ◽  
JERRY W. NIELSEN
Keyword(s):  
Biochemical Characterization ◽  
Microtiter Plate ◽  
Original Method ◽  
Bacterial Isolates ◽  
Plate Method ◽  
Solid Media ◽  
Us Fda ◽  
Term Storage ◽  
Microtiter Plate Format

A microtiter plate format previously reported for the biochemical characterization of microorganisms was modified by adding agar to all of the different test media instead of using a combination of liquid and solid media. This modification, termed the Modified Microtiter Plate procedure (MMP), offered the same advantages of the original method (labor saving, inexpensive, and custom designed for special needs) as well as having the added advantages of longer term storage prior to use and ease of handling. In this study, 60 bacterial isolates (both Listeria suspects and known Listeria cultures) were biotyped using the MMP protocol and compared to results obtained using the classical tube-based US-FDA recommended protocol as the standard identification method and the BBL-Minitek system.

scholarly journals Evaluation of antibacterial activity and molecular characterization of bacteria from Holothuria atra intestine collected from anthropogenic and non-anthropogenic region in Karimunjawa, Indonesia

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Bambang Sulardiono ◽  
NINIEK WIDYORINI ◽  
DJOKO SUPRAPTO ◽  
DIAH AYUNINGRUM ◽  
ARIF RAHMAN
Keyword(s):  
Antibacterial Activity ◽  
Molecular Characterization ◽  
Molecular Identification ◽  
Sea Cucumber ◽  
Bacterial Isolates ◽  
Plate Method ◽  
Agar Plug ◽  
16S Rdna Pcr ◽  
Holothuria Atra

Abstract. Sulardiono B, Widyorini N, Suprapto D, Ayuningrum D, Rahman A. 2020. Evaluation of antibacterial activity and molecular characterization of bacteria from Holothuria atra intestine collected from anthropogenic and non-anthropogenic region in Karimunjawa, Indonesia. Biodiversitas 21: 3149-3155. Evaluation of antibacterial activity and molecular characterization of bacteria from the intestine of Holothuria atra is needed in the anthropogenic region of Menjangan Besar, Karimunjawa. The research aims to evaluate antibacterial activity and molecular characterization of bacteria from intestine of Holothuria atra collected from anthropogenic and non-anthropogenic regions in Karimunjawa, Indonesia. Sea cucumber samples were collected at Menjangan Besar waters as anthropogenic region (code of HM) and Alang-alang waters as non-anthropogenics region (code of HA), Karimunjawa National Park, Indonesia. The H. atra sample collection was using purposive sampling method. Examination of the bacteria from isolation until molecular characterisation was done at the Tropical Marine Biotechnology Laboratory, Uiversitas Diponegoro. The isolation process was conducted using spread plate method, followed by bacterial isolates purification using streak plate method. Screening of antibacterial activity using the agar plug method, as well as molecular identification was conducted by 16S rDNA PCR amplification. The results of this study indicated that a total of 26 bacterial strains were successfully isolated from sea cucumber intestine, consisted of eleven bacterial isolates from H. atra in Menjangan Besar waters and the rest fifteen isolates from H. atra in Alang-alang waters. The preliminary assay of antibacterial test showed H. atra in both waters have potential bacterial isolates. Those bacterial isolates with antibacterial activity were HM1.2 and HA1.1, which based on molecular identification, showed isolates HM1.2 and HA1.1 had the closest similarity with Bacillus paramcoides and Vibrio alginolyticus consecutively, with BLAST homology 98% and 99%. The accession number for both isolates were LC550090 and LC550089, respectively.

scholarly journals CELLULOLYTIC BACTERIA MANGROVE LEAF LITTER IN BANGKA ISLAND

2018 ◽  
Vol 9 (1) ◽  
pp. 06-11
Author(s):  
Ardiansyah Kurniawan ◽  
Asep Awaludin Prihanto ◽  
Suci Puspitasari ◽  
Andi Kurniawan ◽  
Euis Asriani ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Leaf Litter ◽  
Agar Medium ◽  
Cellulose Degradation ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Clear Zone ◽  
Biochemical Tests ◽  
Positive Isolate ◽  
Degradation Ability

The study aimed to obtain isolate of cellulolytic bacteria from leaf litter on mangrove in Bangka Island. Sampling was conducted on mangroves in Sungailiat, Bangka and Tukak Sadai, South Bangka district. The isolation was carried out using 1% enriched agarmedia of Carboxymetyl Cellulose (CMC). The bacterial isolates were tested with cellulolytic growth on 1% enriched CMC agar medium and lugol added at 72 hours. The clear zone resultingindicates cellulose degradation ability. The positive isolate of  cellulolyticwas identified by biochemical tests. 1 of 5 isolates in Sungailiat positive cellulolytic mangroves and identified as Bacillus subtilis. 2 of 5 isolates in mangrove Tukak Sadai is positive as a cellulolytic bacteria and identified as Staphylococcus saproviticus and Bacillus cereus.

scholarly journals KARAKTERISASI BAKTERI PATOGEN PENYEBAB LAYU PADA TANAMAN BAWANG MERAH (Allium ascalonicum L.)

2020 ◽  
Vol 14 (1) ◽  
pp. 69-75
Author(s):  
Intan Fuji Arriani ◽  
Abdul Latief Abadi ◽  
Luqman Qurata Aini
Keyword(s):  
Pathogenic Bacteria ◽  
Economic Value ◽  
Wilt Disease ◽  
Biochemical Test ◽  
Nutrient Agar ◽  
Test Results ◽  
Bacterial Isolates ◽  
Bacterial Isolation ◽  
Identification Of Bacteria

Shallot (Allium ascalonicum L.) is one of the vegetable commodities in the form of tubers that have a high economic value. The development of shallot cultivation in Indonesia often experiences obstacles, one of which is an obstacle in the process of shallot cultivation, namely the attack of Plant Disturbing Organisms (OPT). Information about diseases caused by pathogenic bacteria is still very limited. This study aims to determine the symptoms and identification of pathogenic bacteria that cause wilt in shallots. Bacterial isolation was carried out using Nutrient Agar (NA) media and 36 bacterial isolates were collected from shallots. Bacterial isolates were then tested for pathogenicity to determine the ability of bacteria to cause wilt disease in shallots. The results of isolation obtained 10 bacterial isolates that can show symptoms on red onions namely wilted leaves, yellow and soft rotten tubers. Four isolates including positive can show hypersensitivity symptoms, namely M11, N20, N17 and N14. Based on the identification of bacteria in physiology showed 2 groups of different isolates. Biochemical test results of Isolate M11, N20 and show species suspected of B. cepacia. N3 and N14 isolates are suspected to be E. carotavora subsp. carotavora. The bacterial isolates N7, N17, P5 and P7 were suspected to be E. carotavora subsp. betavasculorum. The isolate of N4 bacteria is suspected to be E. cacticida.

scholarly journals Bacterial Isolation Which is Potential as Cellulosa Pedegradation in Coastal Performance In Tanjungpinang City

2019 ◽  
Vol 20 (2) ◽  
pp. 70-83
Author(s):  
Iwan Iskandar ◽  
Hevi Horiza
Keyword(s):  
Experimental Research ◽  
Cellulose Degradation ◽  
Liquid Waste ◽  
Field Observations ◽  
Bacterial Isolates ◽  
Bacterial Isolation ◽  
Black Water ◽  
Degrading Bacteria ◽  
Coastal Soils ◽  
The City

Many black water or feces and urine field observations in Tanjung Pinang City were dumped directly into the ditches, rivers, and sea. This proves that the management of domestic liquid waste in the City of Tanjungpinang, especially black water, has not yet received serious attention, resulting in a decline in environmental quality. One of the stages in processing black water is decomposition. The decomposition process can be assisted by bacteria, one of which is cellulose-degrading bacteria. This study aims to find bacterial isolates that have the potential to degrade cellulose in feces from coastal soils in Tanjung Pinang City. This research is a laboratory experimental research. The results of this study were obtained 16 bacterial isolates that can degrade cellulose. From these 16 isolates, 3 genera of bacteria were obtained, namely Pseudomonas, Acetobacter and Acinetobacter. Keywords: Cellulose Degradation Bacteria, Coastal Soil  

scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI PENDEGRADASI SELULOSA ASAL EKOSISTEM MANGROVE TUKAK SADAI, BANGKA SELATAN

2018 ◽  
Vol 1 (2) ◽  
pp. 9
Author(s):  
Ardiansyah Kurniawan ◽  
Dwi Febrianti ◽  
Suci Puspita Sari ◽  
Asep Awaludin Prihanto ◽  
Euis Asriani ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Mangrove Ecosystem ◽  
Rrna Gene ◽  
Test Results ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Gram Negative ◽  
Degrading Bacteria ◽  
Cellulose Digestibility

Perikanan dan Kelautan, Universitas Brawijaya3Dosen Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya.ABSTRACTAgricultural waste has problems on fiber and cellulose digestibility in its utilization foraquaculture. The ability of plant cellulose degrading bacteria to become a source ofenergy can increase the digestibility of feed by fish. The purpose of this study was toidentify the cellulolytic bacteria from the mangrove ecosystem, Tukak Sadai District,South Bangka Regency. This study was conducted in March until August 2017.Samples were taken from litter, mud and weathered wood and isolated usingCarboxymetyl Cellulosa (CMC) 1% media and found 22 bacterial isolates. Gramstaining results showed that 3 isolates (TSS 2, TSL 6 and TSK 4) were classified asgram positive and 19 other isolates were gram negative. Cellulolytic test results showed6 isolates had the ability to degrade cellulase, namely 3 isolates from mangrove mudsamples (TSS 1, TSL 7, TSL 1), 2 isolates from leaf litter (TSS 4, TSL 2) and 1 isolatefrom weathered wood (TSK 5) . Sequencing of 16S rRNA gene DNA showed proximityto Pseudomonas aeruginosa in TSL 7 and TSS isolates 4

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