scholarly journals Bacterial Isolation Which is Potential as Cellulosa Pedegradation in Coastal Performance In Tanjungpinang City

2019 ◽  
Vol 20 (2) ◽  
pp. 70-83
Author(s):  
Iwan Iskandar ◽  
Hevi Horiza
Keyword(s):  
Experimental Research ◽  
Cellulose Degradation ◽  
Liquid Waste ◽  
Field Observations ◽  
Bacterial Isolates ◽  
Bacterial Isolation ◽  
Black Water ◽  
Degrading Bacteria ◽  
Coastal Soils ◽  
The City

Many black water or feces and urine field observations in Tanjung Pinang City were dumped directly into the ditches, rivers, and sea. This proves that the management of domestic liquid waste in the City of Tanjungpinang, especially black water, has not yet received serious attention, resulting in a decline in environmental quality. One of the stages in processing black water is decomposition. The decomposition process can be assisted by bacteria, one of which is cellulose-degrading bacteria. This study aims to find bacterial isolates that have the potential to degrade cellulose in feces from coastal soils in Tanjung Pinang City. This research is a laboratory experimental research. The results of this study were obtained 16 bacterial isolates that can degrade cellulose. From these 16 isolates, 3 genera of bacteria were obtained, namely Pseudomonas, Acetobacter and Acinetobacter. Keywords: Cellulose Degradation Bacteria, Coastal Soil  

Isolation of Pathogenic Bacteria from some Male Barbershops in the City of Nasiriyah

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Fadil G. Alswedi ◽  
Amany S H Jaber
Keyword(s):  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Bacterial Species ◽  
Bacterial Isolates ◽  
Bacterial Isolation ◽  
Potential Pathogen ◽  
Colonial Morphology ◽  
Spread Of Infection ◽  
The City

A study was conducted to evaluate bacterial contamination in hairdressing and beauty salons in Thi-Qar at Alnasiriyah city. Samples were collected from ten different salons. The samples were collected from scissors,comb, razor, dryer, sink, and table tools. The isolates obtained were examined and identified using microscopic examination, colonial morphology and biochemical characteristics. Six bacterial species were isolated and identified. The bacterial isolates include Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus spp, Micrococcus Spp,Enterococusspp and Enterobacter. Isolated from some men's salons and shaving tools and the highest frequency of bacteria was in the salon of Nawras by 15%, Salon Ahmed by 12.5% , more bacterial isolation of shaving tools,sink,razor and the presence of this potential pathogen is an indication that hairdressing salons could be contributing to the spread of infection.

scholarly journals Isolasi Dan Karakterisasi Bakteri Selulolitik Biopori Sebagai Upaya Awal Percepatan Proses Pengomposan

2020 ◽  
Vol 4 (1) ◽  
pp. 48-54
Author(s):  
Wardha Maulidya Pratiwi ◽  
Rana Yumna Nabila ◽  
Heliza Amalini ◽  
Guntur Trimulyono
Keyword(s):  
Cellulose Degradation ◽  
Methyl Cellulose ◽  
Test Results ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Bacterial Isolation ◽  
Plate Method ◽  
Yellow Pigmentation ◽  
Ferment Lactose

The purpose of this study was to obtain cellulolytic bacterial isolates isolated from leaf litter in absorption holes biopori FMIPA Unesa and obtain the most optimal cellulolytic bacterial isolates in cellulose degradation. This research was an observational study and the data were analyzed descriptively. Stages of the study began with bacterial isolation, cellulolytic ability testing, and characterization of cellulolytic bacterial isolates. Bacterial isolation was carried out by the pour plate method, isolation was carried out by the streak plate method, cellulolytic testing was carried out using Carboxy Methyl Cellulose media which was given Congo red 1%. While the isolation characterization was done morphologically, physiologically, and biochemically. The results obtained 15 isolates of cellulolytic bacteria that were tested for their ability to degrade cellulose. Cellulolytic test results showed that 6 isolates, namely BS1, BS7, BS10, BS11, BS14, and BS15 had a cellulolytic index of 0.8, 0.8, 0.8, 0.8, 0.8, 0.8. and 1. Isolates BS15 is the most optimal isolate in cellulose degradation with characteristics of the punctiform colony, yellow pigmentation, entire edge, convex elevation, optical opaque and smooth surface, produces catalase enzymes, is non-motile and can ferment in glucose and starch but cannot ferment lactose and also a Gram-negative

Concerning the Concept of Light-Colour Arrangement of the Urban Environment in the Central Part of Tyumen

2020 ◽  
pp. 34-42
Author(s):  
Nikolay I. Shchepetkov ◽  
Svetlana B. Kapeleva ◽  
Denis V. Bugaev ◽  
Gregory S. Matovnikov ◽  
Anna S. Kostareva
Keyword(s):  
Urban Environment ◽  
Comprehensive Analysis ◽  
Assessment Criteria ◽  
Field Observations ◽  
General Plan ◽  
Design Solutions ◽  
Methodological Principles ◽  
The City

The article provides a comprehensive analysis of outdoor lighting in the central part of Tyumen (with consideration of conducted field observations) and prospects of its development on the basis of the general plan of illumination of the central part of the city being under design. Main provisions of this general plan as well as methodological principles and assessment criteria of design solutions illustrat-ed by photographs, schemes and visualisations of the illuminated objects are described.

Biodegradation of organophosphorus Pollutants by Soil Bacteria: Biochemical Aspects and Unsolved Problems

2020 ◽  
Vol 36 (4) ◽  
pp. 126-135
Author(s):  
T.V. Shushkova ◽  
D.O. Epiktetov ◽  
S.V. Tarlachkov ◽  
I.T. Ermakova ◽  
A.A. Leontievskii
Keyword(s):  
Genome Sequencing ◽  
Soil Bacteria ◽  
Activity Regulation ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Russian Science ◽  
Degrading Bacteria ◽  
Phosphorus Source ◽  
Glyphosate Herbicide ◽  
Enzymatic Pathways

The degradation of persistent organophosphorus pollutants have been studied in 6 soil bacterial isolates and in 3 bacterial strains adapted for utilization of glyphosate herbicide (GP) under laboratory conditions. Significant differences in the uptake of organophosphonates were found in taxonomically close strains possessing similar enzymatic pathways of catabolism of these compounds, which indicates the existence of unknown mechanisms of activity regulation of these enzymes. The effect of adaptation for GP utilization as a sole phosphorus source on assimilation rates of several other phosphonates was observed in studied bacteria. The newly found efficient stains provided up to 56% of GP decomposition after application to the soil in the laboratory. The unresolved problems of microbial GP metabolism and the trends for further research on the creation of reliable biologicals capable of decomposing organophosphonates in the environment are discussed. organophosphonates, glyphosate, biodegradation, bioremediation, C-P lyase, phosphonatase, degrading bacteria Investigation of phosphonatase and genome sequencing were supported by Russian Science Foundation Grant no. 18-074-00021.

Modeling of pollution from the wastewater discharge of the city of Limassol

1995 ◽  
Vol 32 (9-10) ◽  
pp. 197-204
Author(s):  
G. C. Christodoulou ◽  
I. Ioakeim ◽  
K. Ioannou
Keyword(s):  
Field Observations ◽  
Wastewater Discharge ◽  
Dispersion Models ◽  
Preliminary Assessment ◽  
Westerly Winds ◽  
Dimensional Finite Element ◽  
The Impact ◽  
The City ◽  
Oceanographic Conditions ◽  
Continuous Loading

The paper presents a numerical modeling study aimed at a preliminary assessment of the impact of the planned sea outfall of the city of Limassol, Cyprus, on the waters of Akrotiri bay. First the local meteorological and oceanographic conditions as well as the loading characteristics are briefly reviewed. Two-dimensional finite element hydrodynamic and dispersion models are subsequently applied to the study area. The results of the former show an eastbound flow pattern under the prevailing westerly winds, in general agreement with available field observations. The spread of BOD and N under continuous loading is then examined for eastward as well as for westward flow as an indicator for the extent of pollution to be expected. The computed concentrations are generally low and confined to the shallower parts of the bay.

scholarly journals Broad diversity of bacteria degrading 17ß-estradiol-3-sulfate isolated from river sediment and biofilm at a wastewater treatment plant discharge

2021 ◽  
Author(s):  
Tamara Mainetti ◽  
Marilena Palmisano ◽  
Fabio Rezzonico ◽  
Blaž Stres ◽  
Susanne Kern ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
River Sediment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Aquatic Environments ◽  
Bacterial Isolates ◽  
Degrading Bacteria ◽  
17Β Estradiol ◽  
Aquatic Wildlife ◽  
Harmful Effects

AbstractConjugated estrogens, such as 17β-estradiol-3-sulfate (E2-3S), can be released into aquatic environments through wastewater treatment plants (WWTP). There, they are microbiologically degraded into free estrogens, which can have harmful effects on aquatic wildlife. Here, the degradation of E2-3S in environmental samples taken upstream, downstream and at the effluent of a WWTP was assessed. Sediment and biofilm samples were enriched for E2-3S-degrading microorganisms, yielding a broad diversity of bacterial isolates, including known and novel degraders of estrogens. Since E2-3S-degrading bacteria were also isolated in the sample upstream of the WWTP, the WWTP does not influence the ability of the microbial community to degrade E2-3S.

Isolation and Characterization of Cellulose Degrading Bacteria of Termite Gut from North Eastern Region of India

2016 ◽  
Vol 5 (6) ◽  
pp. 283-290
Author(s):  
Sonika Sharma ◽  
Soumya Chatterjee ◽  
Sibnarayan Datta ◽  
Rajesh Kumar Prasad ◽  
Angkita Sharma ◽  
...  
Keyword(s):  
Agar Plate ◽  
Bacterial Consortium ◽  
Cellulase Production ◽  
Eastern Region ◽  
Bacterial Isolates ◽  
Carbohydrate Utilization ◽  
North East ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Termite Gut

A study was conducted to screen and isolate cellulase producing bacteria of termite gut from North east region of India. A total of 27 culturable bacterial isolates were screened for cellulase production. Out of the 27 bacterial isolates 11 showed zone of clearance on CMC agar media on staining with 1% Congo red, suggesting potential cellulose degrading activity. The maximum hydrolysis capacities (HC value) on CMC agar plate was found within the range of 3.6 to 40mm. The morphological characterization and gram staining of the positive isolates indicated that 3 isolates were positively stained rods and others were negative cocci. All the cellulase positive isolates were also tested for carbohydrate utilization with maltose, dextrose and fructose, to which all the 11 isolates responded positively. Further, based on the amplification and sequencing of the 16S rRNA genetic region, isolates were identified as member of the genus Bacillus, Paenibacillus and Staphylococcus. The degrading potential of these bacteria were assessed by developing bacterial consortium and efficient degradation was reported after seven days of incubation with different cellulose source like rice, cotton and rice husk.

scholarly journals Cadmium (Cd) Resistance of Isolate Bacteria from Poboya Gold Mining in Palu, Central Sulawesi

2020 ◽  
Vol 20 (2) ◽  
pp. 298
Author(s):  
Fahruddin Fahruddin ◽  
Syahruddin Kasim ◽  
Eka Ummi Rahayu
Keyword(s):  
Heavy Metals ◽  
Mining Industry ◽  
Plate Count ◽  
Resistance Testing ◽  
Bacterial Isolates ◽  
Bacterial Isolation ◽  
Cadmium Resistance ◽  
Cadmium Metal ◽  
Plate Count Method ◽  
Central Sulawesi

The mining industry in Indonesia is growing rapidly, giving an environmental impact from the waste produced because it contains dangerous heavy metals, one of which is cadmium metal (Cd). The purpose to know the ability of cadmium resistance in bacterial isolates. Bacterial isolates were obtained from soil samples from the Poboya Gold Mine field, Palu. Bacterial isolation and selection were carried out by the plate count method, resistance testing using nutrient broth media containing heavy metals Cd, analysis of Cd concentrations using atomic absorption spectrophotometers. From the results of the study, four types of Cd resistant isolates were obtained based on macroscopic and microscopic characterization results, namely isolates EK1, EK2, EK3 and EK4. The two best isolates of Cd resistance, EK2 and EK4 isolates were tested on media containing 20 ppm Cd, showing that both of these isolates were able to significantly reduce Cd concentrations in the supernatant and pellets.

scholarly journals Isolation, Morphological and Biochemical Characterization of Rhizobacteria from Arsenic Contaminated Paddy Soils in Bangladesh: An In vitro Study

2021 ◽  
pp. 41-55
Author(s):  
M. M. Hossain ◽  
G. K. M. M. Rahman ◽  
M. A. M. Akanda ◽  
A. R. M. Solaiman ◽  
M. T. Islam ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Growth And Development ◽  
Phosphate Solubilization ◽  
Cellulose Degradation ◽  
Paddy Soils ◽  
Growth Enhancement ◽  
Bacterial Isolates ◽  
Plant Growth And Development ◽  
Iaa Production

Soil-plant–microbes relations within the plant rhizosphere are the determinants of plant and soil health, which is important for soil ecological environment for plant-microbe interactions. Plant growth-promoting rhizobacteria (PGPR) are considered to encourage plant growth and development directly or indirectly in soil. PGPR can demonstrate a diversity of characteristics responsible .for influencing plant growth and development. During this study, Twenty four different bacterial isolates were isolated, and detailed morphological, biochemical, and physiological characterizations of those isolates were accomplished. This experiment was performed with the 24 bacterial isolates to see their gram stain test, KOH test, catalase activity, cellulose degradation capability, in dole acetic acid (IAA) production, and phosphate solubilization activities, and also tested for growth within the different arsenic and salt stress conditions and 37°C temperature. Results revealed that among the rhizobacterial isolates, fifteen bacterial isolates were negative and nine was positive in gram reaction, while some were showed high IAA production ability, phosphate solubility capability, and cellulose degradation capacity within the culture media. The isolates were isolated from paddy soils and a few were characterized by a yellow color, flat elevation, and gram-positive, while some were characterized because of the yellowish color with round colony shape, raised elevation, gram-negative, and every one the isolates were positive in catalase production capacity and phosphate solubilization activity which is able to increase the available phosphorus within the soil for plants and also produced indole acetic acid that may use as a hormone to be used in growth enhancement of plants. Hence, these isolates need to be tested further for their effect on arsenic dynamics at the plant rhizosphere, selection of suitable plant species for the bacterial association, bacterial effect on arsenic uptake by plants, and potentials for field applications for sustainable agriculture.

scholarly journals Isolation and Identification of Hydrocarbon-Degrading Bacteria that Tolerant to Saponin of Sapindus Rarak Plant

2019 ◽  
Vol 4 (1) ◽  
pp. 79-88
Author(s):  
Evi Octaviany ◽  
Suharjono Suharjono ◽  
Irfan Mustafa
Keyword(s):  
Crude Oil ◽  
16S Rdna ◽  
Bacterial Isolates ◽  
High Concentration ◽  
Isolation And Identification ◽  
Rdna Sequences ◽  
Degrading Bacteria ◽  
16S Rdna Sequences ◽  
Petroleum Contaminated Soil ◽  
Cell Densities

A commercial saponin as biosurfactant can reduce the surface tension of water and increase of hydrocarbon degradation. However, this saponin can be toxic to some hydrocarbonoclastic bac-teria. This study aimed to obtain bacterial isolates that were tolerant and incapable to degrade saponin, and to identify them based on 16S rDNA sequence. Bacteria were isolated from petroleum contaminated soil in Wonocolo Village, Bojonegoro Regency, East Java, Indonesia. The soil samples were acclimated using Bushnell-Haas (BH) broth with 0.5% crude oil at room temperature for 3 weeks. The culture was spread onto BH agar incubated at 30°C for 7 days. The first screened, isolates were grown in nutrient broth with addition of sap-onin 0%, 8%, and 12% (v/v) then incubated at 30°C for three days. The bacterial cell density was measured using a spectrophotometer. Second screened, the isolates were grown on BH broth with addition of 0.5% saponin as a sole carbon source, and their cell densities were measured. The selected isolates were identified based on 16S rDNA sequences. Among 34 bacterial isolates, nine isolates were tol-erant to 12% saponin. Three bacterial isolates IHT1.3, IHT1.5, and IHT3.24 tolerant to high concentration of saponin and did not use this substance as growth nutrition. The IHT1.3, IHT1.5, and IHT3.24 isolates were identified as Ochrobactrum pseudogrignonense (99% similarity), Pseudomonas mendocina (99%), and Ochrobactrum pi-tuitosum; (97%), respectively. Those three selected isolates are good candidates as hydrocarbon-degrading bacteria to bioremediation of soil contaminated crude oil. However, the combined activity of bacteria and saponin to degrade hydrocarbon needs further study. 

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