scholarly journals KARAKTERISASI BAKTERI PATOGEN PENYEBAB LAYU PADA TANAMAN BAWANG MERAH (Allium ascalonicum L.)

2020 ◽  
Vol 14 (1) ◽  
pp. 69-75
Author(s):  
Intan Fuji Arriani ◽  
Abdul Latief Abadi ◽  
Luqman Qurata Aini
Keyword(s):  
Pathogenic Bacteria ◽  
Economic Value ◽  
Wilt Disease ◽  
Biochemical Test ◽  
Nutrient Agar ◽  
Test Results ◽  
Bacterial Isolates ◽  
Bacterial Isolation ◽  
Identification Of Bacteria

Shallot (Allium ascalonicum L.) is one of the vegetable commodities in the form of tubers that have a high economic value. The development of shallot cultivation in Indonesia often experiences obstacles, one of which is an obstacle in the process of shallot cultivation, namely the attack of Plant Disturbing Organisms (OPT). Information about diseases caused by pathogenic bacteria is still very limited. This study aims to determine the symptoms and identification of pathogenic bacteria that cause wilt in shallots. Bacterial isolation was carried out using Nutrient Agar (NA) media and 36 bacterial isolates were collected from shallots. Bacterial isolates were then tested for pathogenicity to determine the ability of bacteria to cause wilt disease in shallots. The results of isolation obtained 10 bacterial isolates that can show symptoms on red onions namely wilted leaves, yellow and soft rotten tubers. Four isolates including positive can show hypersensitivity symptoms, namely M11, N20, N17 and N14. Based on the identification of bacteria in physiology showed 2 groups of different isolates. Biochemical test results of Isolate M11, N20 and show species suspected of B. cepacia. N3 and N14 isolates are suspected to be E. carotavora subsp. carotavora. The bacterial isolates N7, N17, P5 and P7 were suspected to be E. carotavora subsp. betavasculorum. The isolate of N4 bacteria is suspected to be E. cacticida.

Isolation of Pathogenic Bacteria from some Male Barbershops in the City of Nasiriyah

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Fadil G. Alswedi ◽  
Amany S H Jaber
Keyword(s):  
Staphylococcus Epidermidis ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Bacterial Species ◽  
Bacterial Isolates ◽  
Bacterial Isolation ◽  
Potential Pathogen ◽  
Colonial Morphology ◽  
Spread Of Infection ◽  
The City

A study was conducted to evaluate bacterial contamination in hairdressing and beauty salons in Thi-Qar at Alnasiriyah city. Samples were collected from ten different salons. The samples were collected from scissors,comb, razor, dryer, sink, and table tools. The isolates obtained were examined and identified using microscopic examination, colonial morphology and biochemical characteristics. Six bacterial species were isolated and identified. The bacterial isolates include Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus spp, Micrococcus Spp,Enterococusspp and Enterobacter. Isolated from some men's salons and shaving tools and the highest frequency of bacteria was in the salon of Nawras by 15%, Salon Ahmed by 12.5% , more bacterial isolation of shaving tools,sink,razor and the presence of this potential pathogen is an indication that hairdressing salons could be contributing to the spread of infection.

scholarly journals Isolasi Dan Karakterisasi Bakteri Selulolitik Biopori Sebagai Upaya Awal Percepatan Proses Pengomposan

2020 ◽  
Vol 4 (1) ◽  
pp. 48-54
Author(s):  
Wardha Maulidya Pratiwi ◽  
Rana Yumna Nabila ◽  
Heliza Amalini ◽  
Guntur Trimulyono
Keyword(s):  
Cellulose Degradation ◽  
Methyl Cellulose ◽  
Test Results ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Bacterial Isolation ◽  
Plate Method ◽  
Yellow Pigmentation ◽  
Ferment Lactose

The purpose of this study was to obtain cellulolytic bacterial isolates isolated from leaf litter in absorption holes biopori FMIPA Unesa and obtain the most optimal cellulolytic bacterial isolates in cellulose degradation. This research was an observational study and the data were analyzed descriptively. Stages of the study began with bacterial isolation, cellulolytic ability testing, and characterization of cellulolytic bacterial isolates. Bacterial isolation was carried out by the pour plate method, isolation was carried out by the streak plate method, cellulolytic testing was carried out using Carboxy Methyl Cellulose media which was given Congo red 1%. While the isolation characterization was done morphologically, physiologically, and biochemically. The results obtained 15 isolates of cellulolytic bacteria that were tested for their ability to degrade cellulose. Cellulolytic test results showed that 6 isolates, namely BS1, BS7, BS10, BS11, BS14, and BS15 had a cellulolytic index of 0.8, 0.8, 0.8, 0.8, 0.8, 0.8. and 1. Isolates BS15 is the most optimal isolate in cellulose degradation with characteristics of the punctiform colony, yellow pigmentation, entire edge, convex elevation, optical opaque and smooth surface, produces catalase enzymes, is non-motile and can ferment in glucose and starch but cannot ferment lactose and also a Gram-negative

scholarly journals BAKTERI PATOGEN PADA KEPITING KELAPA (Birgus latro)

2021 ◽  
Vol 8 (1) ◽  
pp. 57
Author(s):  
Muhammad Aris ◽  
Sudirto Malan
Keyword(s):  
Pathogenic Bacteria ◽  
Biochemical Characterization ◽  
Economic Value ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Fishery Resource ◽  
Colony Type ◽  
Coastal Plants ◽  
The World ◽  
Steep Slopes

Coconut crab (Birgus latro) is a fishery resource with high economic value. Coconut Crab is only found in eastern Indonesia, covering Sulawesi, Maluku and North Maluku. One of the habitat of Coconut Crab in North Maluku is Moor Island, Central Halmahera. The coastal typology of this island is predominantly rocky with many gaps and small caves, as well as several steep slopes with dominant vegetation conditions of coconut, beach pandanus and other coastal plants. Walnut Crab is often used as a consumption ingredient because it has delicious taste and high nutritions. However, the use of crustaceans for consumption can also be dangerous. This is because crustaceans also contain several pathogenic bacteria. This study aimed to identify pathogenic bacteria isolated from Coconut Crab which is the largest crustacean in the world. 5 samples of Coconut Crab were taken from Moor Island. Isolated samples marked KK1, KK2, KK3, KK4 and KK5. Pure bacterial isolates were obtained from Coconut Crab samples, evaluated for colony type and identified based on biochemical characterization. The types of pathogenic bacteria identified in samples KK1, KK2, KK3, KK4 and KK5 were Escherichia coli, Pseudomonas sp. and Staphylococcus sp.

Transporters affecting biochemical test results: Creatinine-drug interactions

2016 ◽  
Vol 100 (5) ◽  
pp. 437-440 ◽  
Author(s):  
X Chu ◽  
K Bleasby ◽  
GH Chan ◽  
I Nunes ◽  
R Evers
Keyword(s):  
Drug Interactions ◽  
Biochemical Test ◽  
Test Results

A Microbiologic Approach to the Investigation of Bacterial Nosocomial Infection Outbreaks

1980 ◽  
Vol 1 (06) ◽  
pp. 391-400 ◽  
Author(s):  
Donald A. Goldmann ◽  
Ann B. Macone
Keyword(s):  
Nosocomial Infection ◽  
Susceptibility Testing ◽  
Resistant Bacteria ◽  
Antibiotic Susceptibility Testing ◽  
Bacterial Isolates ◽  
Infection Control Team ◽  
Antibiotic Resistant ◽  
Plasmid Analysis ◽  
Identification Of Bacteria ◽  
Bacteriocin Typing

This article details the appropriate microbiologic support that is critical to the successful investigation of nosocomial infection problems. The infection control team must have ready access to microbiologic data, and the laboratory should retain epidemiologically relevant bacterial isolates. Investigation of epidemics is facilitated by precise identification of bacteria and careful antibiotic susceptibility testing. In some situations, biotyping, serotyping, phage typing, bacteriocin typing, and other specialized techniques may be required. Plasmid analysis may be useful in the investigation of nosocomial infection problems caused by antibiotic-resistant bacteria.

MALDI-TOF MS-Based Identification of Bacteria and a Survey of Fresh Vegetables with Pathogenic Bacteria in Beijing, China

2020 ◽  
pp. 100746
Author(s):  
Xiaomin Xu ◽  
Guangyang Liu ◽  
Xiaodong Huang ◽  
Lingyun Li ◽  
Huan Lin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Fresh Vegetables ◽  
Identification Of Bacteria ◽  
Beijing China ◽  
Tof Ms

scholarly journals Bakteri Endofit Tanaman Jeruk Nipis (Citrus aurantifolia) Penghasil Asam Indol Asetat (AIA)

2020 ◽  
Vol 13 (2) ◽  
pp. 179-191
Author(s):  
Oktira Roka Aji ◽  
Iva Dita Lestari
Keyword(s):  
Acetic Acid ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Biochemical Analysis ◽  
Crop Productivity ◽  
Bacterial Identification ◽  
Test Results ◽  
Bacterial Isolates ◽  
Characteristic Analysis ◽  
Citrus Aurantifolia

AbstrakBakteri endofit hidup dalam suatu tanaman tanpa menyebabkan gangguan bagi tanaman yang berperan penting dalam menstimulasi pertumbuhan tanaman, yaitu dengan memproduksi fitohormon seperti asam absisat, asam indol asetat, dan sitokinin. Penelitian ini bertujuan untuk mengisolasi, menyeleksi, dan mengidentifikasi bakteri endofit yang terdapat pada daun, batang, dan akar tanaman jeruk nipis (Citrus aurantifolia). Isolat bakteri endofit diseleksi berdasarkan kemampuannya dalam menghasilkan asam indol asetat (AIA). Isolat bakteri endofit ditumbuhkan pada media nutrient broth (NB) yang ditambah dengan L-triptofan. Konsentrasi AIA dihitung dengan penambahan reagen salkowski dan diukur menggunakan spektrofotometer pada panjang gelombang 530 nm. Identifikasi bakteri endofit dilakukan dengan analisis uji biokimia. Isolat bakteri endofit yang berhasil diisolasi sebanyak 12 isolat, yaitu 4 isolat dari daun, 4 isolat dari batang, dan 4 isolat dari akar. Hasil pengamatan pada uji AIA menunjukkan bahwa semua isolat bakteri endofit dapat menghasilkan hormon AIA. Isolat yang menghasilkan konsentrasi hormon AIA tertinggi adalah isolat B2 (6,51 ppm). Isolat bakteri yang berhasil diidentifikasi berasal dari genus Enterobacter, Bacillus, Pseudomonas, dan Staphylococcus. Bakteri endofit yang dapat menghasilkan AIA berpotensi dikembangkan sebagai biofertilizer untuk meningkatkan produktivitas tanaman. Abstract Endophytic bacteria live inside plants without causing disruption to plants and play an important role in stimulating plant growth. This study aims to isolate endophytic bacteria from lime plant (Citrus aurantifolia) and characterize its ability to produce indole acetic acid (IAA). Bacterial isolates were grown on media supplemented with L-tryptophan as IAA precursor. The bacterial supernatant was mixed with salkowski reagents and then measured using a spectrophotometer at 530 nm. Bacterial identification was carried out using biochemical characteristic analysis. A total of 12 endophytic bacterial isolates were successfully isolated from leaves, stem and roots of plants. Quantitative test results showed that all isolates can produce IAA. The highest concentration of IAA was produced by B2 (6.51 ppm). Biochemical analysis indicated that the isolates were from the genus Enterobacter, Bacillus, Pseudomonas and Staphylococcus. Endhophytic bacteria that can produce IAA have the potential to be developed as biofertilizers to increase crop productivity.

scholarly journals “Enviro School" Education House of Waste Utilization to Create Environmental Care Generation

2018 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Lisa Aisa ◽  
Sulthon Arif Rakhman ◽  
Difa Ashmamillah ◽  
Desi Mutiara Fani ◽  
Gilang Aji Pradana
Keyword(s):  
Waste Management ◽  
Economic Value ◽  
Waste Utilization ◽  
Household Waste ◽  
Consumption Pattern ◽  
Test Results ◽  
River Population ◽  
Environmental Care ◽  
The People ◽  
Post Test

Most of the people at Ciaruteun Ilir Village, Cibungbulang Subdistrict, Bogor District are trashing the river. Population growth and consumption pattern change causes the volume, type, and characteristics of existing waste is increasing, especially household waste. Beside from households, there are also a lot of garbage has coming from the agricultural sectors. The main factors causing the community dispose garbage into the river is the unavailability of landfills, awareness and knowledge of the community related to waste management and utilization is still quite low. Seeing these conditions, the team through the Student Creativity Program for Community Service educate how about the management and utilization of waste named enviro school. Enviro school aims to shape the caring behavior of environmental community through environmental education start from an early age, grouping garbage independently, forming the institution of trash bank, and creating creative products from waste through the creation of vermicompost institutions and handicrafts with functional and economic value with target adult people on Ciaruteun Ilir Village RT 05/03. Enviro school has methods through 7 classes, it is pre class, first class, class, green class, brown class, white class, and enviroducation fair. Based on pre test results conducted at the beginning of the program, only 30.90% of people understand the concept of waste management and utulization, then an increase after four months running through the post test of 92.69%. Successfully this program visible from the increased awareness and knowledge about management and utulization of waste, the establishment of enviro trash bank as a structural institution for waste management, increased youth skills in the utilization of organic waste become vermicompost, and increasing skills of mothers in the utilization of anorganic waste.

scholarly journals Design and Build a Multilevel Mace Nutmeg Dryer to Improve the People's Economy

SinkrOn ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 107-112
Author(s):  
Ihsan Ihsan ◽  
Dirja Nur Ilham ◽  
Reza Ade Putra ◽  
Rudi Arif Candra ◽  
Arie Budiansyah
Keyword(s):  
Software Design ◽  
Economic Value ◽  
Research Work ◽  
Test Results ◽  
Drying Process ◽  
Wet Season ◽  
Drying Time ◽  
Agricultural Community ◽  
Design Software

Nutmeg is a source of income for some people in South Aceh, and some types of nutmeg, like mace nutmeg, are of better quality. Mace nutmeg is also an agricultural community with great economic value and benefits for humans, as it can be processed into spices and herbs. A range of products includes nutmeg oil and medicines. The harvesting and drying of mace nutmeg, which is still considered a problem by nutmeg growers, cannot be isolated from the processing of the basic ingredients. The natural process of drying mace nutmeg involves the use of sunshine, which necessitates a considerable drying time. Therefore nutmeg farmers frequently complain of erratic weather, especially during the wet season. The constant rain can cause the mace to rot, causing the nutmeg farming community's revenue to become unstable. Methods and steps of research work starting from the study of literature, determination of design specifications, hardware design, software design, toolmaking, tool testing.Good results were reached with the dryness of the mace nutmeg, which can be adjusted, and without putting into account the weather in the drying process, which is usually done with the heat of the sun, per the results of the testing of the designed tools.The average dryness of mace nutmeg is at a temperature of 45 percent with a time of 4 hours and a capacity of 100 grams; according to the test results of the automatic mace drying machine, it produces 50 grams of dry mace.  

scholarly journals Detection of pathogenic bacteria associated with earphones used by students of Stamford University Bangladesh

2020 ◽  
Vol 10 (1) ◽  
pp. 1-4
Author(s):  
Omor Ahmed Chowdhury ◽  
Md Raihan Ahmed ◽  
Md Raihan Dipu ◽  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Potential Source ◽  
Different Types ◽  
Staphylococcus Spp

The use of earphones has increased in recent times throughout the world especially among the different level of students such as school, college or university who have a higher tendency of sharing these among them. Unlike airline headsets, headphones and stethoscope ear-pieces, ear phones are often shared by multiple users and can be a potential medium for transmission of pathogens, which can give rise to various ear related infections. The objective of this study was to detect the pathogenic bacteria from the ear-phones used by the students of Stamford University Bangladesh. A total of 16 ear-phone swabs were collected by sterile cotton swabs. The swabs were inoculated onto blood agar and incubated aerobically overnight at 37oC. Microscopic observation and standard biochemical tests were performed to confirm the identification of all the bacterial isolates. Six presumptively identified Staphylococcus spp. (38%) were tested against six different types of antibiotics following Kirby-Bauer disk diffusion method. Isolates were found to be 84% resistant against Cotrimoxazole and demonstrated 100% sensitivity to Vancomycin and Ciprorofloxacin. The findings of this study suggest the users to disinfect their respective ear phones and not to exchange them as they may act as a potential source to transfer pathogenic and antibiotic resistant bacteria among the ear phone users. Stamford Journal of Microbiology, Vol.10 (1) 2020: 1-4

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