scholarly journals Pengaruh lama perendaman menggunakan air kelapa terhadap maskulisasi ikan lele masamo (Clarias sp)

2019 ◽  
Vol 6 (2) ◽  
pp. 58
Author(s):  
Suardi Laheng ◽  
Anjar Widyastuti
Keyword(s):  
Treatment Efficacy ◽  
Immersion Time ◽  
Coconut Water ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels

AbstrakTujuan penelitian untuk mengetahui bagaimana proses maskulinisasi pada ikan lele masamo dan bagaimana hasil maskulinisasi ikan lele masamo menggunakan air kelapa. Penelitian ini dilaksanakan di Balai Benih Ikan Lokal Tatatanga, Kota Palu. Hewan uji yang digunakan dalam penelitian ini yaitu larva ikan lele masamo (Clarias sp) berjumlah 360 ekor berumur 7 hari. Rancangan penelitian yang digunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan tiga kali ulangan, yaitu : P1 (lama waktu perendaman 5 jam), P2 (lama waktu perendaman 10 jam), P3 (lama waktu perendaman 15 jam) dan P4 (lama perendaman 20 jam). Larva ikan Lele masamo berumur 7 hari direndam dalam air kelapa dengan dosis 30%/L. Pengamatan dilakukan pada umur ikan 40 hari. Hasil penelitian menunjukkan rata-rata jumlah persentase ikan lele jantan pada perlakuan P1; P2; P3 dan P4 masing-masing adalah 53,89; 65,56; 44,44 dan 0,00%. Perlakuan P2 efektif dalam maskulinisasi ikan lele masamo melalui perendaman air kelapa.Kata kunci: maskulinisasi; lele masamo; air kelapa; pertumbuhanAbstractThis present work aimed to investigate the masculinization of masamo catfish (Clarias sp) larvae by immersion with coconut water. The experiment was carried out in local fish nurtury centre in Tatatanga, Palu.  The 7-days-old larvae (360 individuals) of masamo catfish was used. A completely randomized design was arranged, using four levels of immersion time:  5, 10, 15, and 20 (named as P1, P2, P3, and P4, respectively). The immersion was carried out in coconut water and made three triplicates. After the fish reached 40 days old, the treatment efficacy was evaluated. The results showed that the percentage of male catfish differed sharply, i.e. 53,89% (P1), 65,56% (P2), 44,44% (P3), and 0,00% (P4). To sum up, we concluded that P2 demonstrated the highest percentage, being the most effective masculinization treatment on masamo catfish.Keywords: masculinization; masamo catfish; coconut water; growth

PENGARUH PENAMBAHAN EKSTRAK JAGUNG MANIS DAN AIR KELAPA PADA MEDIA MS SECARA IN VITRO TERHADAP INDUKSI TUNAS ADVENTIF BAWANG PUTIH (Allium sativum L.)

2021 ◽  
Vol 23 (2) ◽  
pp. 92-97
Author(s):  
Marisa Gracia Bakara ◽  
Makhziah Makhziah ◽  
Guniarti Guniarti
Keyword(s):  
Allium Sativum ◽  
Sweet Corn ◽  
Water Concentration ◽  
Coconut Water ◽  
Time Treatment ◽  
Concentration Time ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels

[SWEET CORN EXTRACT AND COCONUT WATER EFFECT TO INDUCE GARLIC (Allium sativum L.) ADVENTIST SHOOTS BY IN-VITRO CULTURE]. Garlic (Allium sativum L.) is one of the horticultural plants used daily for various uses. This study aims to determine the best extract concentration of sweet corn and coconut water and its interaction for induction garlic Adventist shoots (Allium sativum L.) var. Lumbu Hijau. The study was conducted at the Biotechnology Laboratory, Agriculture Faculty, UPN ”Veteran” East Java, in February – April 2021. The experimental design used was a factorial completely randomized design (CRD). The first factor is four levels of extract of sweet corn concentration (0, 50, 100, and 150 g/L), while the second factor is four levels of coconut water concentration (0, 50, 100, and 150 mL/L). The results showed that the extract of sweet corn concentration-time treatment increased the growing adventist shoots percentage, adventist shoots number, and adventist shoots size. The section of sweet corn concentration of 150 g/L increased the rate of growing adventist shoots, adventist shoots number, and adventist shoots size. The treatment of coconut water concentration and its interaction with the extract of sweet corn concentration is still not able for induction garlic adventist shoots (Allium sativum L.) of the var. Lumbu Hijau.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

KARAKTERISTIK FISIK BUBUR INSTAN TERSUBTITUSI TEPUNG PISANG TONGKA LANGIT

2015 ◽  
Vol 4 (2) ◽  
pp. 41-44
Author(s):  
Priscillia Picauly ◽  
Gilian Tetelepta
Keyword(s):  
Water Solubility ◽  
Physical Property ◽  
Rice Flour ◽  
Absorption Index ◽  
Randomized Design ◽  
Nutrition Value ◽  
Banana Flour ◽  
Completely Randomized Design ◽  
Four Levels

Instant porridge is sereal-based food that can be combined with fruits so it will contain better nutrition. To improve the nutrition value of instant porridge then substitute of one kind of banana originally from Maluku is Tongka langit banana. A good quality of instant porridge has a high nutrition and a best physical property. This research aims to characterize of the physical properties instant porridge that are substituted by Tongka langit banana flour and the rice flour. The design that is applied is completely randomized design with four levels of treatment in comparing the substitution between Tongka langit banana flour and the rice flour as follow 20%, 40%, 60% and 80%. According to the result of this research, the physical characteristic of instant porridge that are substituted by Tongka langit banana flour are bulk density (0.84-0.89 g/mL), water absorption index (3.49-4.05%), and water solubility index (0.02-0.04%).

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

scholarly journals POTENSI EKSTRAK DAUN KARUK (Piper sarmentosum) SEBAGAI INSEKTISIDA NABATI HAMA ULAT GRAYAK (Spodoptera litura)

EKOLOGIA ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 55-62
Author(s):  
Rangga Eka S. P ◽  
Moerfiah . ◽  
Triastinurmiatiningsih .
Keyword(s):  
Spodoptera Litura ◽  
Larval Growth ◽  
Mortality Rates ◽  
Average Growth Rate ◽  
Average Growth ◽  
Extract Concentration ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Extent Of Damage

The purpose of this study was to determine the activity of the leaf extract of Piper sarmentosum on mortality and damage levels by pest armyworm (Spodoptera litura). This research was conducted at the Laboratory of Biology, Faculty of Mathematics and Natural Sciences, Pakuan University, Bogor. This study uses a completely randomized design (CRD), with four levels of extract concentration, that is 0% as control, 30%, 40% and 50%.  Each treatment was repeated four times. The variables measured were mortality rates, the extent of damage the leaves, and larval growth. A concentration of EDK 50% showed activity in killing the armyworm by 38%. The lowest level of damage is 5%, with an average growth rate to its lowest larvae by 0.8 cm with the provision of treatment EDK 50%, and the higest level of damage is 75% by a concetration of EDK 30% treatments.

scholarly journals PENGARUH JENIS PELARUT TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK MENIRAN (Phyllanthus niruri L.)

2020 ◽  
Vol 9 (4) ◽  
pp. 458
Author(s):  
Rizky Amalia Rachmawati ◽  
Ni Wayan Wisaniyasa ◽  
I Ketut Suter
Keyword(s):  
Antioxidant Activity ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Flavonoid Content ◽  
Phyllanthus Niruri ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Effect On Yield ◽  
The Right

This study was conducted to determine the effect of different solvents on the antioxidant activity of gale of the wind extract and to find out the right type of solvent used to extract gale of the wind with the highest antioxidant activity using maceration method. The experimental design used in this study was a completely randomized design (CRD) with a type of solvent as a treatment. The treatment consisting of four levels, namely methanol, ethanol, acetone, isopropanol. All treatments were repeated four times so that they were obtained 16 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect followed by Duncan test. The results showed that the type of solvent treatment had a very significant effect on yield, total phenol, total flavonoids, total tannin, and antioxidant activity (IC50). The results showed that acetone solvent had the best treatment which produced a yield of 22.58%, total phenol content of 188.77 mg GAE/g, total flavonoid content of 247.60 mg QE/g, total tannin content of 297.51mg TAE/g, and antioxidant activity (IC50) of 15.19 ppm.

scholarly journals Effects of the growth regulators for the induction of somatic embryos from explants in an endemic and threatened Echinocactus parryi Engelm.

2021 ◽  
Vol 27 (3) ◽  
pp. 431-447
Author(s):  
Dolores Adilene García-González ◽  
◽  
María del Socorro Santos-Díaz ◽  
Juan Pedro Flores-Margez ◽  
Pedro Osuna-Ávila ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Somatic Embryos ◽  
Histological Analysis ◽  
Globular Stage ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Green Callus ◽  
Dichlorophenoxy Acetic Acid ◽  
Mature Seeds

Introduction: The list of threatened species is enhancing and needs to be revised by integrating plant tissue culture tools with conventional techniques that support the appropriate management of these species. Objective: To assess the effects of the growth regulators for the induction of somatic embryos from mature seeds, shoots, and compact green callus of Echinocactus parryi Engelm. and the histological analysis of the embryogenic structures. Materials and methods: A completely randomized design was utilized to evaluate three types of explants (apical, medium, and basal) cultured on basal Murashige & Skoog media (MS) with different growth regulators concentrations (2, 4-D [dichlorophenoxy acetic acid], BAP [6-benzylaminopurine] and kinetin, at four levels: 0.5, 1, 1.5, and 2 mg∙L -1 ). Histological analysis of the embryogenic structures was performed. Results and discussion: The 2, 4-D induced both embryogenic and organogenic callus from seeds and shoot explants. The globular stage did not evolve to their maturity, presumably because of 2, 4-D accumulation. The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant when they were cultured in the medium with 0.5 mg∙L -1 kinetin. However, the latest phases did not germinate, probably due to abnormalities generated by genetic and epigenetic changes in the DNA that can cause abnormal somatic embryos. The histology image demonstrated that the globular and torpedo structures were visible under a microscope showing stained nucleus and numerous starch grains. Conclusions: E. parryi is a species that can produce a high number of embryogenic structures, which represents a great potential to grow massive plants.

scholarly journals THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 39
Author(s):  
Salma Nabila ◽  
Endah Budi Irawati ◽  
Rina Srilestari
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Fresh Weight ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety  of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on  fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

scholarly journals PEMBERIAN STIMULAN PADA BIDANG SADAP DALAM MEMPENGARUHI PRODUKSI DAN KUALITAS LATEKS TANAMAN KARET (Hevea brasiliensis (Muell. Arg.)

2019 ◽  
Vol 4 (1) ◽  
pp. 38
Author(s):  
Hayata Hayata ◽  
Yulistiati Nengsih ◽  
Rahmanto Wibowo
Keyword(s):  
Quality Control ◽  
Ash Content ◽  
The Other ◽  
Rubber Content ◽  
Randomized Design ◽  
Treatment Factor ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Latex Production

Production increasing of latex on rubber plants can be increased by using a stimulants. One of it  is Vicar 10 SL. The purpose of this study was to find out the effect of Vicar 10 SL on the production and quality of latex. This research was carried out in the Kasang Parit Village, Sekernan District, Muaro Jambi Regency, and the Goods Quality Control and Certification Center on Industry and trade department   in Jambi Province. The study was conducted in July to August 2017. The design used was a completely randomized design with one treatment factor as the Vicar 10 SL application with four levels of treatment, namely; Without treatment (V0/control), 1 ml/Tree (V1), 2 ml/tree (V2), 3 ml/tree (V3). There were 4 times  repeatation on each treatment. Using  fingers, a Vicar 10 SL solution was applied to the tapping groove which was adjusted to the treatment, and allowed to keep it 24 hours. Tapping is done in the next morning, by following the grooves and be stored in a cup and left until the latex stoped dripping. Vicar was given once a week during 30 days. Tapping was done 3 times a week. The variables observed were latex production (gram/tree/day), dry rubber content (%), latex ash content (%), and latex dirt content (%). Vicar giving 10 SL in the tapping site with a dose of 2 ml / tree gave the highest yield of latex production (93.38 grams / tree / day) and dry rubber content (75.50%) and was significantly different compared to the other treatments. Vicar giving 10 SL in tapping site had no significant effect on latex dirt content and latex ash contentKeywords: Latex, stimulant, product and quality Abstrak Peningkatan produksi lateks pada tanaman karet dapat ditingkatkan dengan menggunakan stimulan. Salah satu pemakaian yang digunakan adalah Vikar 10 SL. Tujuan penelitian ini adalah untuk megetahui penggunaan stimulan pada bidang sadap dalam mempengaruhi produksi dan kualitas lateks. Penelitian ini dilakukan di Desa kasang Parit Kecamatan Sekernan Kabupaten Muaro Jambi, dan Balai Pengujian dan Sertifikasi Mutu Barang (BPSMB) Disperindag Propinsi Jambi. Penelitian dilakukan pada bulan Juli sampai dengan Agustus 2017. Rancangan yang digunakan adalah Rancangan Acak Lengkap dengan satu faktor perlakuan pemberian stimulan (Vikar 10 SL) dengan empat taraf perlakuan  yaitu; Tanpa perlakuan (V0/kontrol),  1 ml/ Pohon (V1),  2 ml/pohon (V2), 3 ml/pohon  (V3). Setiap perlakuan diulang sebanyak 4 kali. Dengan menggunakan jari dioleskan larutan Vikar 10 SL pada alur sadap  yang disesuaikan dengan perlakuan, dan didiamkan selama 24 jam. Penyadapan dilakukan pada pagi hari esoknya, dengan mengikuti alur torehan dan ditampung dalam cawan dan dibiarkan sampai lateks berhenti menetes. Pemberian Vikar dilakukan sekali 7 hari selama 30 hari. Penyadapan dilakukan  3 kali dalam seminggu. Peubah yang diamati adalah produksi lateks (gram/pohon/hari)), kadar karet kering (%), kadar abu lateks (%), kadar kotoran lateks (%). Pemberian stimulan (Vikar 10 SL) pada bidang sadap dengan dosis 2 ml/pohon memberikan hasil yang tertinggi terhadap produksi lateks 93,38 gram/pohon/hari dan kadar karet kering 75,50 % dan berbeda nyata bila dibandingkan dengan perlakuan lainnya. Pemberian stimulan (Vikar 10 SL) pada bidang sadap berpengaruh tidak nyata terhadap kotoran lateks dan kadar abu lateksKata kunci :Lateks, stimulan, produksi dan kualitas

scholarly journals Morphogenesis and structure of Tifton 85 cultivated in subtropical climate and fertilized with nitrogen

2016 ◽  
Vol 38 (4) ◽  
pp. 389 ◽  
Author(s):  
Diego Daniel Tiecher ◽  
Marta Gomes da Rocha ◽  
Luciana Pötter ◽  
Paulo Roberto Salvador ◽  
Tuani Lopes Bergoli ◽  
...  
Keyword(s):  
Regression Model ◽  
Nonlinear Regression Model ◽  
Subtropical Climate ◽  
Degree Days ◽  
Leaf Lifespan ◽  
Experimental Animals ◽  
Sward Height ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels

The experiment evaluated the morphogenesis and structure of Tifton 85 (Cynodon spp.) cultivated in subtropical climate and fertilized with nitrogen (N). The experiment was a completely randomized design with four levels of N (Zero; 75; 150 or 225 kg ha-1) in nine replicates per area. The experimental animals were Suffolk female lambs. The grazing method was continuous to maintain the sward height at 15 cm ± 10%. The stem expansion increased by 0.000027 cm degree-days-1 to each kg N applied (linear model). According to nonlinear model, the highest stem expansion (0.0226 cm degree-days-1) was observed with the use 220.1 kg ha-1 N. The leaf lifespan fitted a linear regression model, with increase of 50 degree-days leaf -1, comparing the levels zero and 225 kg ha-1 of N. According to nonlinear regression model, the longest leaf lifespan (407.1 degree-days) was observed with the use 208.8 kg ha-1 N. With the maintenance of sward height at approximately 15 cm, we recommended to use 200 kg ha-1 N in Tifton 85 cultivated in subtropical climate. 

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