scholarly journals A SIX PLATE MICROBIOLOGICAL ASSAY FOR SCREENING OF ANTIMICROBIAL RESIDUES IN FOOD OF ANIMAL ORIGIN

2020 ◽  
Vol 8 (11) ◽  
pp. 22-28
Author(s):  
Madhavi H. Hathurusinghe ◽  
Ruwan C. Gunasena ◽  
Preeni Abeynayake
Keyword(s):  
Screening Test ◽  
Micrococcus Luteus ◽  
Low Cost ◽  
Health Concern ◽  
Animal Origin ◽  
Indicator Organisms ◽  
Consumer Safety ◽  
Food Of Animal Origin ◽  
Antimicrobial Residues ◽  
Food Commodities

Occurrence of antimicrobial residues in livestock and aquatic products is a current public health concern. The monitoring of food commodities for antimicrobial residues will ensure the consumer safety and promote trade. Therefore, the objective of this study was to establish a screening test to detect antimicrobial residues in food of animal origin. A bioassay consists of six plates was established, which enables screening of six groups of antimicrobials, namely; penicillins, sulphonamides, streptomycin, erythromycin, tetracyclines and fluroquinolones. The Six Plate Test (SPT) is a bioassay technique, which is carried out using Bacillus subtilis BGA (Merck), Bacillus cereus (ATCC 11778), Micrococcus luteus (ATCC 9341) and Escherichia coli (ATCC 11303) as indicator organisms. The Mueller Hinton Agar (MHA) was used as the test medium at optimum pH for all indicator organisms except B.cereus, for which the Media No.8 was used. The Minimum Detectable Concentrations (MDC) of SPT were determined using serial dilutions of antibiotics. All the MDC values obtained on SPT for the validated antimicrobials were below the recommended MRL of each antimicrobial. SPT can be developed to a quick, easy to perform, robust and low cost screening test to detect antimicrobial residues in food of animal origin.

scholarly journals Contribution of Nanomaterials to the Development of Electrochemical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 69
Author(s):  
Valérie Gaudin
Keyword(s):  
Signal Amplification ◽  
Electrochemical Sensors ◽  
Low Cost ◽  
Food Products ◽  
Animal Origin ◽  
Screening Methods ◽  
Chemical Methods ◽  
Portable Instrumentation ◽  
Physico Chemical ◽  
Antimicrobial Residues

The detection of antimicrobial residues in food products of animal origin is of utmost importance. Indeed antimicrobial residues could be present in animal derived food products because of animal treatments for curative purposes or from illegal use. The usual screening methods to detect antimicrobial residues in food are microbiological, immunological or physico-chemical methods. The development of biosensors to propose sensitive, cheap and quick alternatives to classical methods is constantly increasing. Aptasensors are one of the major trends proposed in the literature, in parallel with the development of immunosensors based on antibodies. The characteristics of electrochemical sensors (i.e., low cost, miniaturization, and portable instrumentation) make them very good candidates to develop screening methods for antimicrobial residues in food products. This review will focus on the recent advances in the development of electrochemical aptasensors for the detection of antimicrobial residues in food products. The contribution of nanomaterials to improve the performance characteristics of electrochemical aptasensors (e.g., Sensitivity, easiness, stability) in the last ten years, as well as signal amplification techniques will be highlighted.

scholarly journals A Proof of Principle for the Detection of Viable Brucella spp. in Raw Milk by qPCR Targeting Bacteriophages

2020 ◽  
Vol 8 (9) ◽  
pp. 1326
Author(s):  
Michaela Projahn ◽  
Jens A. Hammerl ◽  
Ralf Dieckmann ◽  
Sascha Al Dahouk
Keyword(s):  
Limit Of Detection ◽  
Raw Milk ◽  
Qpcr Assay ◽  
Animal Origin ◽  
Indicator Organisms ◽  
Food Of Animal Origin ◽  
Global Health Issue ◽  
Contaminated Food ◽  
Viable Bacteria ◽  
And Performance

Brucellosis is still a global health issue, and surveillance and control of this zoonotic disease in livestock remains a challenge. Human outbreaks are mainly linked to the consumption of unpasteurized dairy products. The detection of human pathogenic Brucella species in food of animal origin is time-consuming and laborious. Bacteriophages are broadly applied to the typing of Brucella isolates from pure culture. Since phages intracellularly replicate to very high numbers, they can also be used as specific indicator organisms of their host bacteria. We developed a novel real-time PCR (qPCR) assay targeting the highly conserved helicase sequence harbored in all currently known Brucella-specific lytic phages. Quality and performance tests determined a limit of detection of <1 genomic copy/µL. In raw milk artificially contaminated with Brucella microti, Izv phages were reliably detected after 39 h of incubation, indicating the presence of viable bacteria. The qPCR assay showed high stability in the milk matrix and significantly shortened the time to diagnosis when compared to traditional culture-based techniques. Hence, our molecular assay is a reliable and sensitive method to analyze phage titers, may help to reduce the hands-on time needed for the screening of potentially contaminated food, and reveals infection risks without bacterial isolation.

scholarly journals Antimicrobial Resistance and Virulence Genes inEnterococcus faeciumandEnterococcus faecalisfrom Humans and Retail Red Meat

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Majda Golob ◽  
Mateja Pate ◽  
Darja Kušar ◽  
Urška Dermota ◽  
Jana Avberšek ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Red Meat ◽  
Health Concern ◽  
Animal Origin ◽  
Common Pattern ◽  
Exact Test ◽  
Microdilution Method ◽  
Food Of Animal Origin ◽  
Broth Microdilution Method ◽  
Human Infections

The emergence of antimicrobial-resistant and virulent enterococci is a major public health concern. While enterococci are commonly found in food of animal origin, the knowledge on their zoonotic potential is limited. The aim of this study was to determine and compare the antimicrobial susceptibility and virulence traits ofEnterococcus faecalisandEnterococcus faeciumisolates from human clinical specimens and retail red meat in Slovenia. A total of 242 isolates were investigated: 101 from humans (71E. faecalis, 30E. faecium) and 141 from fresh beef and pork (120E. faecalis, 21E. faecium). The susceptibility to 12 antimicrobials was tested using a broth microdilution method, and the presence of seven common virulence genes was investigated using PCR. In both species, the distribution of several resistance phenotypes and virulence genes was disparate for isolates of different origin. All isolates were susceptible to daptomycin, linezolid, teicoplanin, and vancomycin. In both species, the susceptibility to antimicrobials was strongly associated with a food origin and the multidrug resistance, observed in 29.6% ofE. faecalisand 73.3%E. faeciumclinical isolates, with a clinical origin (Fisher’s exact test). Among meat isolates, in total 66.0% ofE. faecalisandE. faeciumisolates were susceptible to all antimicrobials tested and 32.6% were resistant to either one or two antimicrobials. InE. faecalis, several virulence genes were significantly associated with a clinical origin; the most common (31.0%) gene pattern included all the tested genes excepthyl. In meat isolates, the virulence genes were detected inE. faecalisonly and the most common pattern includedace,efaA, andgelE(32.5%), of whichgelEshowed a statistically significant association with a clinical origin. These results emphasize the importance ofE. faecalisin red meat as a reservoir of virulence genes involved in its persistence and human infections with reported severe outcomes.

scholarly journals Pesticide Residues in Selected Food Commodities: Results from the Danish National Pesticide Monitoring Program 1995-1996

1999 ◽  
Vol 82 (2) ◽  
pp. 337-358 ◽  
Author(s):  
René K Juhler ◽  
Milter Green Lauridsen ◽  
Mette Rindom Christensen ◽  
Gudrun Hilbert
Keyword(s):  
Fruits And Vegetables ◽  
Monitoring Program ◽  
Animal Origin ◽  
Chlorinated Pesticides ◽  
Animal Products ◽  
Pesticide Monitoring ◽  
Food Of Animal Origin ◽  
Fish Samples ◽  
Food Commodities ◽  
Meat Samples

Abstract The paper presents results of analyses of 4182 samples collected from January 1995 to December 1996 for the Danish National Pesticide Monitoring Program. The program basis is a random sample control supplemented with a target control. The objectives of the control are to monitor consumer exposure to health hazards and to enforce national and European Community regulations. The Danish Veterinary and Food Administration is responsible for establishing methods, data manipulation, and evaluation, but actual analyses are performed at 4 regional laboratories. In addition to a description of the Danish National Pesticide Monitoring Program on food, the effects of lowering the reporting limits are discussed. Pesticides included are those in current use as well as chlorinated pesticides like lindane, DDT, and HCB, occurring in food now primarily as a result of environmental contamination. Commodities analyzed are fruits, vegetables, cereals, bran, fish, and animal products such as meat, butter, cheese, fat, and eggs. In fruits and vegetables, residues were detected in 10% of 2515 samples, with higher incidences of detection for foreign commodities. Violation rate was 0.6%. In food of animal origin, low levels of organo- chlorine pesticides were detected in most fish samples and in more than half of the animal product samples. However, no results exceeding maximum residue limits were found. No residues of organo- phosphorus pesticides were detected in the 231 meat samples analyzed.

scholarly journals Impact of residues of veterinary medicinal products in food of animal origin on public health safety in Poland in the years 2003-2017

2020 ◽  
Vol 76 (06) ◽  
pp. 6412-2020
Author(s):  
MICHAŁ MAJEWSKI ◽  
KRZYSZTOF ANUSZ ◽  
ZBIGNIEW BEŁKOT ◽  
PRZEMYSŁAW RACEWICZ ◽  
ANNA ŁUKOMSKA
Keyword(s):  
Public Health ◽  
Radioactive Contamination ◽  
Annual Reports ◽  
Animal Origin ◽  
Antibiotic Residues ◽  
Medicinal Products ◽  
Consumer Safety ◽  
Food Of Animal Origin ◽  
Waiting Periods ◽  
Veterinary Medicinal Products

Monitoring the presence of residues of unauthorized substances, chemical and biological residues, medicinal products and radioactive contamination in food is an important element of the system for protection of consumers against hazardous foods. The aim of the study was to analyze the results of monitoring tests for the presence of antibiotics and chemotherapeutics affecting consumer safety in products of animal origin that were carried out in Poland in the years 2003-2017. The material for analysis were annual reports on the results of tests for the presence of prohibited substances, chemical and biological residues, medicinal products and radioactive contamination in food produced in Poland. Samples for testing were taken at random from food of animal origin produced on farms under the supervision of the Veterinary Inspectorate. The reports present data on monitoring tests carried out in the years 2003-2017. The analysis was based on the results for B1 substances, which include antibacterial substances, antibiotics and chemotherapeutics approved for use in food-producing animals. The results from the last two years of research were analyzed in detail, including the results for prohibited substances with antibacterial action from group A6 (prohibited substances) and, in the case of poultry, for coccidiostats (group B2b).The results of the monitoring studies in Poland indicate that both veterinarians and animal breeders were aware that the proper use of chemotherapeutics and compliance with waiting periods are necessary to ensure food safety. Samples that exceeded the standards represented less than 0.3% of all samples tested in all types of food. According to the results of these studies, antibiotic residues were most frequently found in honey.

scholarly journals Environmental contamination of free-range hen with dioxin

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Szczepan Mikołajczyk ◽  
Marek Pajurek ◽  
Małgorzata Warenik-Bany ◽  
Sebastian Maszewski
Keyword(s):  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Animal Origin ◽  
Surveillance Program ◽  
Congener Profile ◽  
Free Range ◽  
Consumer Safety ◽  
Food Of Animal Origin ◽  
Frequent Consumption ◽  
Waste Burning

Abstract Introduction The transfer of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and polychlorinated biphenyls (PCBs) from a contaminated environment into the food chain is a serious consumer safety problem. As part of the Polish National Surveillance Program of PCDD/Fs and PCBs in food of animal origin, a concentration of PCDD/Fs of 4.61 ± 0.75 pg WHO-TEQ/g fat was determined in a sample of free-range eggs, which exceeded the permitted limit of 2.5 pg WHO-TEQ/g. The aim of the study was to investigate the source of the egg contamination and the risk for the eggs’ consumers. Materials and methods Eggs, muscles, feed and soil from the place where backyard waste burning had been carried out in the past and ash from a household stove tipped onto the paddock were analysed using the isotope dilution technique with high-resolution gas chromatography coupled with high-resolution mass spectrometry. Results The concentration in ash was low at 0.20 pg WHO-TEQ/g and the congener profile did not indicate the source of contamination. The dioxin content in soil from the backyard waste-burning site was 2.53 pg WHO-TEQ/g dry matter (d.m.) and the soil’s profile of PCDD/F congeners matched the profile of the contaminated eggs. Conclusion By reason of the congener profile similarity, the investigation concluded, that the cause of the contamination was the backyard waste-burning site soil which the animals had access to. Frequent consumption of contaminated eggs from the analysed farm could pose a health risk due to chronic exposure, especially for vulnerable consumers.

scholarly journals Knowledge and practice of cattle handlers on antibiotic residues in meat and milk in Kwara State, Northcentral Nigeria

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0257249
Author(s):  
Mary Idowu Olasoju ◽  
Taiwo Israel Olasoju ◽  
Oluwawemimo Oluseun Adebowale ◽  
Victoria Olusola Adetunji
Keyword(s):  
Online Survey ◽  
Animal Health ◽  
Health Concern ◽  
Animal Origin ◽  
Human Consumption ◽  
Antibiotic Residues ◽  
Cross Sectional ◽  
Poor Knowledge ◽  
Food Of Animal Origin ◽  
Knowledge And Practice

Objectives Antibiotics are important for improving animal health and production. However, the deposition of its residues in food of animal origin intended for human consumption at non-permissible levels has generated global health concern and the need to tackle this using the “One Health Approach”. This study assessed the knowledge and practice of 286 cattle handlers in Kwara State, Nigeria. Methods A web-based cross sectional online survey using a semi-structured questionnaire was conducted from November to December, 2019. Univariate, bivariate and multivariate analyses were performed at 95% confidence interval to determine predictors of good knowledge and practices towards Antibiotic Residues in Meat and Milk among cattle handlers. Results This study revealed that majority (52.7% n = 165/286) of the cattle handlers were not aware of antibiotic residues. Knowledge and practices regarding antibiotic residues were generally poor among the study population; 36.7% and 35.5% had satisfactory knowledge and practice respectively. The age (p = 0.026), gender (p = 0.006) and business duration (p = 0.001) of participants were significantly associated with their knowledge of antimicrobial residues. The effect of education on knowledge was modified by age. The odds of having poor knowledge on antibiotic residues increased 4 times among participants who were ≤40 years old than those above 40 years (Stratum Specific OR = 3.65; CI = 1.2, 11.1; p = 0.026). Knowledge levels of participants were statistically associated with their practice levels p<0.05 (OR = 2.43; CI = 1.45. 4.06; p = 0.0006). Conclusion This implies that poor knowledge is a risk factor to having poor practice among cattle handlers. Deliberate efforts towards educating cattle farmers on best farm practices in antibiotic use would prevent antibiotic residues in meat and milk. Also, an effective surveillance system for monitoring the use of veterinary drugs in Kwara State, Nigeria is crucial.

scholarly journals Non-Typhoidal Salmonella at the Human-Food-of-Animal-Origin Interface in Australia

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1192
Author(s):  
Hamid Reza Sodagari ◽  
Penghao Wang ◽  
Ian Robertson ◽  
Ihab Habib ◽  
Shafi Sahibzada
Keyword(s):  
Antimicrobial Resistance ◽  
Developed Countries ◽  
Health Concern ◽  
Animal Origin ◽  
Global Public Health ◽  
Human Food ◽  
Salmonella Infections ◽  
Food Of Animal Origin ◽  
Global Epidemiology ◽  
Human Salmonellosis

Non-typhoidal Salmonella is a major zoonotic pathogen that plays a significant role in foodborne human salmonellosis worldwide through the consumption of contaminated foods, particularly those of animal origin. Despite a considerable reduction in human salmonellosis outbreaks in developed countries, Australia is experiencing a continuous rise of such outbreaks in humans. This review of the literature highlights the reported non-typhoidal Salmonella outbreaks in humans as well as the occurrence of the pathogen in foods from animal sources throughout Australia. Non-typhoidal Salmonella infections from food animals are more often associated with at-risk people, such as immunocompromised and aged people or children. Although several animal-sourced foods were recognised as the catalysts for salmonellosis outbreaks in Australia, egg and egg-based products remained the most implicated foods in the reported outbreaks. This review further highlights the antimicrobial resistance trends of non-typhoidal Salmonella isolates at the human–food interface, with a focus on clinically important antimicrobials in humans, by collating evidence from previous investigations in Australia. The rise in antimicrobial-resistant Salmonella, especially to antimicrobials commonly prescribed to treat human salmonellosis, has become a significant global public health concern. However, the overall prevalence of antimicrobial resistance in Australia is considerably lower than in other parts of the world, particularly in terms of critically important antimicrobials for the treatment of human salmonellosis. The present review adds to our understanding of the global epidemiology of non-typhoidal Salmonella with emphasis on the past few decades in Australia.

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