Antioxidant Properties Research of Various Types of Coffee

The article presents results of antioxidant compounds determination (total amount of phenolic compounds, total amount of flavonoids) and parameters of the antiradical activity (by the DPPH method) and reducing power (by the FRAP method) in different types of coffee beans depending on roasting degree (weak, medium, strong), coffee variety (Robusta and Arabica) and the importing country. The researchers used the products presented in the retail chains of Samara. The study purpose was to reveal a universal and most common source of functional substances with an antioxidant effect for the human body and use it both in its pure form and in combination with other products (BAS). According to the research results, Robusta coffee (Coffea Canephora) of medium roasting from Brazil has high indicators for all the conducted analyses and can be used as an additional source of antioxidant substances, and as a raw material for obtaining BAS. Coffee Arabica (Coffea Arabica) of medium roasting of Indonesian origin has the highest restoring power, and the highest content of phenols and flavonoids. Coffee Arabica (Coffea Arabica) of a strong degree of roasting from India has the lowest rates. All other types of coffee have average, slightly different results.

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Studies on drying characteristic, nutritional composition, and antioxidant properties of Stevia rebaudiana (Bertoni) leaves

International Agrophysics ◽

10.1515/intag-2015-0039 ◽

2015 ◽

Vol 29 (3) ◽

pp. 323-331 ◽

Cited By ~ 7

Author(s):

Yolanda B. Moguel-Ordóñez ◽

Diana L. Cabrera-Amaro ◽

Maira R. Segura-Campos ◽

Jorge C. Ruiz-Ruiz

Keyword(s):

Antioxidant Properties ◽

Reducing Power ◽

Stevia Rebaudiana ◽

Biological Properties ◽

Nutritional Composition ◽

Antioxidant Compounds ◽

Drying Methods ◽

Stevia Rebaudiana Bertoni ◽

Trolox Equivalent ◽

Drying Treatment

Abstract Drying characteristics of Stevia rebaudiana (Bertoni) leaves using radiation drying, convection drying, sun drying, and shade drying treatments were investigated in a convective type. Results for all treatments indicated that drying took place in the falling rate period. The chroma of the dried samples ranged between 29.66 and 23.85. Convection and shade drying samples were found better in colour and had the highest content of total pigments. The proximate and fibre composition were affected by the drying treatments. Extracts obtained from convection drying and shade drying leaves exhibited higher values of Trolox equivalent antioxidant capacities 416.89-423.64 mM mg−1 and ferric reducing power 85.19-87.97%. These extracts also showed ion chelating capacity 59.05-60.21% for Cu+2 and 55.05-59.11% for Fe+2. The results indicate that it is possible to select the drying treatment to obtain the best nutritional and biological properties. Convection drying and shade drying were the least aggressive treatments and leaf powders obtained by these drying methods could be used as sources of nutrients, fibre, and antioxidant compounds.

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Determination of Three Main Chlorogenic Acids in Water Extracts of Coffee Leaves by Liquid Chromatography Coupled to an Electrochemical Detector

Antioxidants ◽

10.3390/antiox7100143 ◽

2018 ◽

Vol 7 (10) ◽

pp. 143 ◽

Cited By ~ 4

Author(s):

Rocío Rodríguez-Gómez ◽

Jérôme Vanheuverzwjin ◽

Florence Souard ◽

Cédric Delporte ◽

Caroline Stevigny ◽

...

Keyword(s):

Liquid Chromatography ◽

Coffea Arabica ◽

Limit Of Detection ◽

Coffea Canephora ◽

Antioxidant Compounds ◽

Chlorogenic Acids ◽

Coffea Species ◽

Coffee Species ◽

Coffea Liberica

Coffee is a beverage widely consumed in the world. The coffee species most commercialized worldwide are Arabica (Coffea arabica) and Robusta (Coffea canephora). Roasted coffee beans are the most used, but coffee leaves are also consumed as infusion in several countries for traditional medicinal purposes. They contain several interesting phenolic antioxidant compounds mainly belonging to chlorogenic acids (CGAs). In the present work, a liquid chromatography-electrochemical detection (LC-EC) method was developed for the determination of three main chlorogenic acid isomers, namely 3-, 4-, and 5-caffeoylquinic acids (CQA), in coffee leaves aqueous extracts. Samples from eight coffee species, namely; Coffea arabica, Coffea canephora, Coffea liberica, Coffea humilis, Coffea mannii, Coffea charrieriana, Coffea anthonyi, and Coffea liberica var. liberica, were grown and collected in tropical greenhouses. Linearity of the calibration graphs was observed in the range from the limit of quantification to 1.0 × 10−5 M, with R2 equal to 99.9% in all cases. High sensitivity was achieved with a limit of detection of 1.0 × 10−8 M for 3-CQA and 5-CQA (i.e., 3.5 µg/L) and 2.0 × 10−8 M for 4-CQA (i.e., 7.1 µg/L). The chromatographic profile of the samples harvested for each Coffea species was studied comparatively. Obtained raw data were pretreated for baseline variations and shifts in retention times between the chromatographic profiles. Principal Component Analysis (PCA) was applied to the pretreated data. According to the results, three clusters of Coffea species were found. In the water sample extracts, 5-CQA appeared to be the major isomer, and some species contained a very low amount of CQAs. Fluctuations were observed depending on the Coffea species and harvesting period. Significant differences between January and July were noticed regarding CQAs content. The species with the best CQAs/caffeine ratio was identified. The LC-EC data were validated by liquid chromatography-high resolution mass spectrometry (LC-HRMS).

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Assessment of flavonoids and phenolic compound accumulation in invasive Solidago canadensis L. in Slovakia

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1378 ◽

2020 ◽

Vol 14 ◽

pp. 587-594

Author(s):

Olga Shelepova ◽

Yulia Vinogradova ◽

Olena Vergun ◽

Olga Grygorieva ◽

Ján Brindza

Keyword(s):

Phenolic Compounds ◽

Therapeutic Potential ◽

Radical Scavenging ◽

Reducing Power ◽

Total Flavonoid ◽

Raw Material ◽

Herbal Remedies ◽

Total Phenolic ◽

Solidago Canadensis ◽

Dpph Method

Solidago canadensis L. was introduced to Europe as an ornamental plant from North America in 1645 and began to spread during the XIX-XX centuries. Nowadays the species is considered the most aggressive invasive species. On the other hand, S. canadensis is considered to be a medicinal plant. The raw material known as Herba Solidaginis includes herbs of S. canadensis, S. gigantea, and S. virgaurea. These species are known for their diuretic, anti-inflammatory, antimicrobial, antioxidant, antispasmodic properties. The purpose of our study was to analyze the chemical compounds and some biological properties of S. canadensis, growing in Slovakia, to evaluate its therapeutic potential. The total phenolic content (TPC) of the extracts from aerial parts of S. canadensis was determined by the Folin-Ciocalteu method. The evaluation of total flavonoid content (TFC) was performed by using a spectrophotometric method. The flavonoids content was expressed as rutin equivalents (mg REs) per g DW vegetal product. The phytochemical profile of S. canadensis extracts was assessed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The radical scavenging activity of samples was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Reducing power of extracts was determined by the phosphomolybdenum method. Total phenolic contents (TPC) and total flavonoid contents (TFC) of the extracts varied from 204.19 to 293.43 mg GAE.g-1 DW, and 64.99 – 175.25 g QE.g-1 DW, respectively; the best results were obtained for ethanol extract. Some phenolic compounds were identified by HPLC with significant amounts of rutin (211.20 µg.mL-1), quercetin (122.08 µg.mL-1), quercitrin (102.50 µg.mL-1) and chlorogenic acid (147.00 µg.mL-1). The DPPH values in the inflorescences were higher than in the leaves: the antioxidant activity of leaf extracts was in the range from 5.34 to 17.16 mg TE.g-1, for inflorescences, this parameter ranged from 6.09 to 19.87 mg TE.g-1. The high total phenolic compounds and flavonoids can be used as a valuable source of phytochemicals in herbal remedies. Our study of S. canadensis, growing in Slovakia, shows the promising potential that can be evaluated as an effective antioxidant and antimicrobial agent in herbal medicines.

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Antioxidant Capacity and the Correlation with Major Phenolic Compounds, Anthocyanin, and Tocopherol Content in Various Extracts from the Wild EdibleBoletus edulisMushroom

BioMed Research International ◽

10.1155/2013/313905 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 30

Author(s):

Emanuel Vamanu ◽

Sultana Nita

Keyword(s):

Antioxidant Capacity ◽

Antioxidant Properties ◽

Reducing Power ◽

Ethanolic Extract ◽

Edible Mushroom ◽

Tocopherol Content ◽

Hot Water ◽

Antioxidant Compounds ◽

Methanolic Extracts ◽

Freeze Dried

Boletus edulisis a wild edible mushroom habitually consumed by rural populations. Ethanolic and methanolic extracts was obtained in cold and hot water from dried fruit bodies. The antioxidant activity of freeze-dried extracts fromB. eduliswere investigated using free radicals scavenging activity, reducing power, metal chelating effect, inhibition of lipid peroxidation, and the identification of antioxidant compounds. The levels of different compounds with antioxidant properties were higher in alcoholic extracts compared with aqueous extracts. Rosmarinic acid was the major phenolic compound, it being identified in a concentration between7±0.23and56±0.15 mg/100 g extract. A positive correlation between the content of total phenols, flavonoids, anthocyanins, and tocopherols, and the antioxidant capacity of the extracts was determined. The results showed that the ethanolic extract of Romanian wild mushroomB. edulisrepresents a natural source of functional compounds.

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Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d211020 ◽

2020 ◽

Vol 21 (10) ◽

Author(s):

RIDWANTI BATUBARA ◽

TENGKU ISMANELLY HANUM ◽

ODING AFFANDI ◽

HENNY SRI WAHYUNI

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Chemical Compounds ◽

Raw Material ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Analysis ◽

Mature Leaves ◽

Dpph Method ◽

North Sumatra ◽

Chemical Contents

Abstract. Batubara R, Hanum TI, Affandi O, Wahyuni HS. 2020. Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties. Biodiversitas 21: 4616-4622. The genus Wikstroemia is one of the genus of agarwood producing trees which has not been widely researched and published. One type of this genus is Wikstroemia tenuiramis Miq, which grows naturally in North Sumatra, the leaves are like other types of agarwood leaves can be used as raw material for agarwood leaves tea. A research has been conducted to determine the chemical contents contained in the young and mature leaves of agarwood plants species W. Tenuiramis. This research methods applied qualitative analysis using phytochemical screening, quantitative analysis to determine tannin content using titration method, antioxidant activity analysis using 2,2-diphenil-1-picrylhydrazil (DPPH) method, and compound tracing using Py-gas chromatography-mass spectrometry (Py-GC-MS) instruments. The qualitative phytochemical analysis results showed no difference in chemical compounds contained in both the young and mature leaves. Both the young and mature leaves contained flavonoids, triterpenoids, and tannins, and did not contain alkaloids, steroids and saponins. The young contained tannins of 1.079 ± 0.001% and the mature contained tannins of 4.645 ± 0.021%, not significant statistically. Py-GC-MS analysis showed the presence of various 30 bioactive compounds contained in both young and mature leaves. The analysis results also showed that both the young and mature leaves had very strong antioxidant activity. Our findings suggest that the two types of leaves have equal utility value of in term of chemical contents and antioxidant potentials.

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Phytochemical Constituents, Antioxidant Activity, and Toxicity Assessment of the Aerial Part Extracts from the Infraspecific Taxa of Matthiola fruticulosa (Brassicaceae) Endemic to Sicily

Molecules ◽

10.3390/molecules26144114 ◽

2021 ◽

Vol 26 (14) ◽

pp. 4114

Author(s):

Maria Fernanda Taviano ◽

Emilia Cavò ◽

Vivienne Spadaro ◽

Francesco Maria Raimondo ◽

Vincenzo Musolino ◽

...

Keyword(s):

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Artemia Salina ◽

Toxicity Assessment ◽

Antioxidant Compounds ◽

Volatile Components ◽

Infraspecific Taxa

In a project designed to investigate the specific and infraspecific taxa of Matthiola endemic to Sicily (Italy) as new potential sources of bioactive compounds in this work, the infraspecific taxa of Matthiola fruticulosa were studied, namely, subsp. fruticulosa and subsp. coronopifolia. HPLC–PDA/ESI–MS and SPME–GC/MS analyses of hydroalcoholic extracts obtained from the aerial parts of the two subspecies led to the detection of 51 phenolics and 61 volatile components, highlighting a quite different qualitative–quantitative profile. The antioxidant properties of the extracts were explored through in vitro methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power and Fe2+ chelating activity assays. The results of the antioxidant tests showed that the extracts possess a different antioxidant ability: particularly, the extract of M. fruticulosa subsp. fruticulosa exhibited higher radical scavenging activity than that of subsp. coronopifolia (IC50 = 1.25 ± 0.02 mg/mL and 2.86 ± 0.05 mg/mL), which in turn displayed better chelating properties (IC50 = 1.49 ± 0.01 mg/mL and 0.63 ± 0.01 mg/mL). Lastly, Artemia salina lethality bioassay was performed for toxicity assessment. The results of the bioassay showed lack of toxicity against brine shrimp larvae for both extracts. The data presented indicate the infraspecific taxa of M. fruticulosa as new and safe sources of antioxidant compounds.

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Free radical scavenging activity, total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts

Hemijska industrija ◽

10.2298/hemind111111002r ◽

2012 ◽

Vol 66 (4) ◽

pp. 547-552 ◽

Cited By ~ 36

Author(s):

Marija Radojkovic ◽

Zoran Zekovic ◽

Senka Vidovic ◽

Drago Kocar ◽

Pavle Maskovic

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Phenolic Components ◽

Dpph Method ◽

Morus Spp ◽

Phenolic And Flavonoid

Mulberry (Morus spp. L., Moraceae) fruits, leaves, bark and branch have been used in traditional medicine as diuretic, hypoglycemic and hypotensive. The mechanism of their effects is correlated with the content of active components. Objective of this work was to evaluate and compare antioxidant properties of different extracts of two Morus species growing in Serbia: Morus alba L. (white mulberry) and Morus nigra L. (black mulberry). Potential antioxidant activity, content of antioxidant compounds (phenolics and flavonoids) and radical scavenging capacity, tested by DPPH method, were evaluated. The phenolic and flavonoid composition of different Morus extracts was determined by the HPLC method. The extracts prepared from fruits, leaves and roots of M. alba and M. nigra exhibited different characteristics. The highest extraction yield was achieved by M. alba leaves extraction (23.40%). M. nigra roots extract shown the highest total phenolics (186.30 mg CAE/g), while highest total flavonoids content (67.37 mg RE/g) was determined for M. nigra leaves extracts. In addition, black mulberry leaves extracts with the highest antioxidant activity had the highest phenolic acids contents. The dominant phenolic components in the samples were rutin and chlorogenic acid. All investigated mulberry dry extracts shown high content of phenolic compounds and significant antioxidant activity. This work contributes to knowledge of the antioxidant properties of Morus species. The obtained results may be useful in the evaluation of new dietary supplements and food products.

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Antioxidant activity of plant extracts and their effect on methane fermentation in bioreactors

International Agrophysics ◽

10.1515/intag-2017-0031 ◽

2018 ◽

Vol 32 (3) ◽

pp. 395-401 ◽

Cited By ~ 1

Author(s):

Marta Oleszek ◽

Solomiia Kozachok

Keyword(s):

Antioxidant Activity ◽

Production Efficiency ◽

Biogas Production ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Antioxidant Compounds ◽

Methane Fermentation ◽

Biogas Yield

Abstract Extracts of Solidago virgaurea L. (European goldenrod), Lavandula angustifolia Mill. (lavender) and Arnica chamissonis Less. (arnica) were tested as potential additives for methane fermentation in bioreactors, as it was hypothesized that their antioxidant properties may improve biogas production efficiency. Hence, methane fermentation of maize silage with the addition of tested extracts was performed in eudiometers, and both the biogas volume and methane content in biogas were measured. In addition, antioxidant properties, such as reducing power and 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging activity, were determined spectrophotometrically. The results showed that only the addition of a goldenrod crude extract caused a significant increase in biogas yield, despite the fact that it presented the lowest antioxidant activity of the three tested species. No significant differences in biogas yield were found between the other two additives and the control. Biogas production efficiency was not correlated with the antioxidant activity of the crude extracts. Hence, the increase in methane fermentation efficiency in the case of goldenrod supplementation might have resulted from some other non-antioxidant compounds occurring in this species.

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Antioxidant potential of Pfaffia glomerata and Arrabidaea chica extracts combination

Research Society and Development ◽

10.33448/rsd-v10i4.14150 ◽

2021 ◽

Vol 10 (4) ◽

pp. e33910414150

Author(s):

Lucas Dutra Zani da Silva Souza ◽

Ariana Ferrari ◽

Daniele Fernanda Felipe

Keyword(s):

Synergistic Effect ◽

Antioxidant Properties ◽

Natural Antioxidants ◽

Antioxidant Property ◽

Antioxidant Potential ◽

Antioxidant Compounds ◽

Pfaffia Glomerata ◽

Therapeutic Benefits ◽

Dpph Method ◽

Arrabidaea Chica

Several therapeutic benefits of medicinal plants are attributed to antioxidant properties, since antioxidant compounds play an important role in the prevention of various diseases and aging. Many products have synergistic action from combinations of plant extracts that contain natural antioxidants. The present study aimed to evaluate the synergistic effect of Pfaffia glomerata extract with Arrabidaea chica extract for antioxidant action. In the study the extract of the inflorescences of P. glomerata and the extract of the leaves of A. chica were used. The synergism between P. glomerata extract and A. chica extract was evaluated using the ratio between experimental and theoretical antioxidant activity, which was determined using the DPPH method and the potential total reactive antioxidant method (TRAP). The combination of P. glomerata extract and A. chica extract showed a synergistic effect, in which the antioxidant property of this combination was substantially superior to the sum of the individual antioxidant effects. This demonstrates that the combined extracts can be important for the development of products that have a high antioxidant potential.

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Development and validation of chromatographic methods to quantify organic compounds in green coffee (Coffea arabica) beans

Australian Journal of Crop Science ◽

10.21475/ajcs.20.14.08.p2433 ◽

2020 ◽

pp. 1275-1282

Author(s):

Wilder Douglas Santiago ◽

Alexandre Rezende Teixeira ◽

Juliana de Andrade Santiago ◽

Ana Cláudia Alencar Lopes ◽

Rafaela Magalhães Brandão ◽

...

Keyword(s):

Organic Acids ◽

Chlorogenic Acid ◽

Bioactive Compounds ◽

Coffea Arabica ◽

Limit Of Detection ◽

Raw Material ◽

Green Coffee ◽

Green Coffee Beans ◽

Coffee Beans ◽

Validation Parameters

Coffee is one of the oldest and most consumed beverages. The raw material for obtaining a good quality coffee drink is the grain. Bioactive compounds and organic acids in coffee beans are reflections of a series of attributes that, together, confer to the coffee peculiar flavor and aroma. This study aimed to identify trigonelline, chlorogenic acid, caffeine and organic acids in green coffee beans, and to validate the chromatographic method using High-Performance Liquid Chromatography (HPLC). Coffea arabica green coffee beans were used in this experiment. Trigonelline, chlorogenic acid, caffeine, and organic acids were identified and quantified by HPLC. Selectivity, linearity, limit of detection, limit of quantification, precision, and accuracy were used to validate the methods. Three bioactive compounds (trigonelline, chlorogenic acid, and caffeine) and seven organic acids (oxalic, citric, malic, quinic, succinic, lactic, and acetic acids) were quantified in the samples. The mean values for trigonelline, chlorogenic acid (5-ACQ), and caffeine ranged from 1.28 to 1.66, 3.47 to 4.73 and 1.26 to 1.59 g 100g-1, respectively. The values of organic acids, as well as the phenolic compounds, agree with those reported by the literature for green coffee beans of C. arabica. The validation parameters allowed the method to be considered linear, exact, and precise. Therefore, it resulted in a method reliable for studies of the composition of food matrix or quality control of green coffee beans.

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