scholarly journals Antioxidant potential of Pfaffia glomerata and Arrabidaea chica extracts combination

2021 ◽  
Vol 10 (4) ◽  
pp. e33910414150
Author(s):  
Lucas Dutra Zani da Silva Souza ◽  
Ariana Ferrari ◽  
Daniele Fernanda Felipe
Keyword(s):  
Synergistic Effect ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Antioxidant Property ◽  
Antioxidant Potential ◽  
Antioxidant Compounds ◽  
Pfaffia Glomerata ◽  
Therapeutic Benefits ◽  
Dpph Method ◽  
Arrabidaea Chica

Several therapeutic benefits of medicinal plants are attributed to antioxidant properties, since antioxidant compounds play an important role in the prevention of various diseases and aging. Many products have synergistic action from combinations of plant extracts that contain natural antioxidants. The present study aimed to evaluate the synergistic effect of Pfaffia glomerata extract with Arrabidaea chica extract for antioxidant action. In the study the extract of the inflorescences of P. glomerata and the extract of the leaves of A. chica were used. The synergism between P. glomerata extract and A. chica extract was evaluated using the ratio between experimental and theoretical antioxidant activity, which was determined using the DPPH method and the potential total reactive antioxidant method (TRAP). The combination of P. glomerata extract and A. chica extract showed a synergistic effect, in which the antioxidant property of this combination was substantially superior to the sum of the individual antioxidant effects. This demonstrates that the combined extracts can be important for the development of products that have a high antioxidant potential.

scholarly journals POTENSI SENYAWA BIOAKTIF MESOCARP BUAH LONTAR (Borassus fl abeliffer L.) SEBAGAI SUMBER ANTIOKSIDAN ALAMI (Potency of Mesocarp Bioactive Compounds in Lontar Fruit (Borassus fl abeliffer L.) as A Source of Natural Antioxidant)

2014 ◽  
Vol 34 (03) ◽  
pp. 277 ◽  
Author(s):  
Eny Idayati ◽  
Suparmo Suparmo ◽  
Purnama Darmadji
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Properties ◽  
Chemical Properties ◽  
Natural Antioxidants ◽  
Chromatography Method ◽  
Carotene Content ◽  
Palm Fruit ◽  
Dpph Method ◽  
Total Fat ◽  
Physical And Chemical

This study was aimed to characterize the physical and chemical properties of borassus palm fruit mesocarp, to determine the best type of solvent to extract bioactive compounds by maceration method, and to evaluate the antioxidant properties of bioactive compounds using DPPH (1,1-difenil-2-pikrilhidrazil) method. Carotenoid was separated based on TLC(thin layer chromatography) method, which produced spots. To confi rm the results, the spots were scanned using UV-vis spectrofotometry. The results showed that the water content of borassus fruit mesocarp was 77.31%, while total fat, ash content, and tannin were 0.11%, 1.43%, 0.08%, respectively. Total Carotenoid was 8324.6 µg/100g with β carotene content was 6217.48 microgram/100g. The best solvent used in extracting the bioactive compounds was ethanol and acetone with (1:1) ratio. The highest yield was 4.3% and bioactive compounds in palm fruit mesocarpextracts as antioxidants was about 87% as carried out by DPPH method, so it could become a potential antioxidant. TLC Identifi cation produced two spots. One spot was identifi ed as carotenoids from xanthophyll group and the other one was β-carotene.Keywords: Natural antioxidants, borassus palm fruit mesocarp, bioactive compounds ABSTRAKPenelitian ini bertujuan untuk karakterisasi senyawa bioaktif dalam mesocarp buah lontar berdasarkan sifat fisik dan kimia, mengetahui jenis pelarut terbaik untuk mengekstrak senyawa bioaktif dengan metode maserasi, serta mengevaluasi sifat antioksidan senyawa bioaktif mesocarp dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Dasarpemisahan karotenoid dengan metoda KLT (kromatografi  lapis tipis) pada ekstrak lalu dikuatkan dengan hasil scanning menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa mesocarp buah lontar mengandung kadar air 77,31%; total lemak 0,11%, kadar abu 1,43%; tanin 0,08%; total karotenoid 8324,6 µg/100g dengan kandungan senyawa  karoten 6217,48 µg/100g. Perlakuan pelarut terbaik untuk proses ekstraksi senyawa bioaktif adalah etanol dan aseton dengan rasio (1:1). Hasil rendemen tertinggi yaitu 4,3% dan potensi senyawa bioaktif dalam ekstrak mesocarp buah lontar sebagai antioksidan dengan metode DPPH yaitu sekitar 87%, sehingga berpotensi sebagai salahsatu senyawa antioksidan. Identifi kasi dengan metode KLT yang menghasilkan 2 noda yaitu noda 1 diduga karotenoid dari golongan xantofi l dan noda 2 yatu  karoten.Kata kunci: Antioksidan alami, mesocarp lontar, senyawa bioaktif

scholarly journals Antioxidant Properties Research of Various Types of Coffee

Food Industry ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 74-81
Author(s):  
Dinara Ignatova ◽  
Nadezhda Makarova
Keyword(s):  
Coffea Arabica ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Coffea Canephora ◽  
Raw Material ◽  
Antioxidant Compounds ◽  
Common Source ◽  
Coffee Arabica ◽  
Coffee Beans ◽  
Dpph Method

The article presents results of antioxidant compounds determination (total amount of phenolic compounds, total amount of flavonoids) and parameters of the antiradical activity (by the DPPH method) and reducing power (by the FRAP method) in different types of coffee beans depending on roasting degree (weak, medium, strong), coffee variety (Robusta and Arabica) and the importing country. The researchers used the products presented in the retail chains of Samara. The study purpose was to reveal a universal and most common source of functional substances with an antioxidant effect for the human body and use it both in its pure form and in combination with other products (BAS). According to the research results, Robusta coffee (Coffea Canephora) of medium roasting from Brazil has high indicators for all the conducted analyses and can be used as an additional source of antioxidant substances, and as a raw material for obtaining BAS. Coffee Arabica (Coffea Arabica) of medium roasting of Indonesian origin has the highest restoring power, and the highest content of phenols and flavonoids. Coffee Arabica (Coffea Arabica) of a strong degree of roasting from India has the lowest rates. All other types of coffee have average, slightly different results.

scholarly journals Phenols and Melanoidins as Natural Antioxidants in Beer. Structure, Reactivity and Antioxidant Activity

Biomolecules ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 400 ◽  
Author(s):  
Alvaro Martinez-Gomez ◽  
Isabel Caballero ◽  
Carlos A. Blanco
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Antioxidant Compounds ◽  
Appreciable Effect ◽  
Human Metabolism ◽  
The World ◽  
Maillard Products ◽  
By Products

Beer is one of the most consumed drinks around the world, containing a variety of compounds that offer both appreciated sensorial characteristics and health advantages. Important healthy compounds in beer are those with antioxidant properties that attenuate the content of free radicals produced as by-products in the human metabolism, exerting an appreciable effect against cancers or cardiovascular diseases. This work details a study of antioxidant compounds present in beer, focusing on the two main groups: phenols (including polyphenolic forms) and melanoidins, formed specifically during brewing as Maillard products. The fundaments of the most important methods to evaluate beer antioxidant activity, the main antioxidant compounds present in beer—especially those with healthy properties—and the new trends to increase beer antioxidant activity are also discussed.

scholarly journals Antioxidant potential study of some synthesized N-heterocycles

2009 ◽  
Vol 35 (2) ◽  
pp. 49-52 ◽  
Author(s):  
Mohammad Mamun Hossain ◽  
Sukanta Kumar Shaha ◽  
Foysal Aziz
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Anticancer Activity ◽  
Heterocyclic Compounds ◽  
Antioxidant Properties ◽  
Biological Properties ◽  
Antioxidant Potential ◽  
Heating Method ◽  
Dpph Method ◽  
First Time

Nitrogen containing heterocyclic compounds such as oxindoles especially isatins and their derivatives have excellent biological properties such as anti-inflammatory, antimicrobial, anticancer activity. In addition, this is first time ever we would like to report the antioxidant properties of the said class of compounds. To determine such an important bioactivity a number of halogenated oxindoles 1-3, their diaza-biphenyl derivatives (4-6), ring extended biphenyl (7) and bis-amide (8) were synthesized by classical heating method. The antioxidant activity of all the synthesized compounds was screened by DPPH method with respect to ascorbic acid. In our present investigation some of the synthesized compounds (1, 2, 3, 5 and 6) were found to be active.Keywords: Antioxidant; Isatin; OxindoleOnline 12 August 2009DOI: 10.3329/bmrcb.v35i2.2564Bangladesh Med Res Counc Bull 2009; 35: 49-52 

scholarly journals PHYTOCHEMICALS, ANTIOXIDANT ACTIVITY AND PHENOLIC PROFILING OF DIPLOCYCLOS PALMATUS (L.) C. JEFFERY

2017 ◽  
Vol 9 (4) ◽  
pp. 101 ◽  
Author(s):  
Usmangani A. Attar ◽  
Savaliram G. Ghane
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Reversed Phase ◽  
Antioxidant Potential ◽  
Antioxidant Compounds ◽  
Fruit Extract ◽  
Metal Chelating ◽  
Rp Hplc

Objective: The aim of this study was to analyze phytochemicals, antioxidant potential and phenolic profiling of leaf and fruit extracts of Diplocyclos palmatus.Methods: The leaves and fruits were subjected for sequential extraction with hexane, chloroform, methanol and water. All extracts were subjected to biochemical studies such as phenols, tannins, flavonoids, terpenoids and antioxidant assays such as 1,1-diphenyl-1-picryl hydrazyl (DPPH), 2,2’ Azinobis (3-ethyl-benzothiozoline-6-sulfonic acid (ABTS), ferric reducing antioxidant property (FRAP), metal chelating and phospho- molybdenum reduction assay. Further methanolic extract was used for phenolics characterization by reversed phase-high performance liquid chromatography (RP-HPLC).Results: It was observed that methanol fruit extract showed significantly higher phenolics (9.29±0.01 mg tannic acid equivalent (TAE)/g extract), flavonoids (15.02±0.96 mg catechin equivalent (CE)/g extract) and terpenoids (276.73±0.76 mg ursolic acid equivalent (UAE)/g extract). However, chloroform extracts of leaf and fruit exhibited a high amount of tannins (22.07±0.06, 6.99±0.10 mg CE/g extract) respectively. The extracts were subjected to assess their antioxidant potential using various in vitro systems such as DPPH, ABTS, FRAP, metal chelating and phospho- molybdenum reduction. Among the various extracts, methanol fruit extract had highest DPPH radical scavenging activity (26.73±0.14 mg ascorbic acid equivalent (AAE)/g extract), metal chelating activity (0.80±0.01 mg EDTA equivalent (EE)/g extract) and phospho- molybdenum activity (291.24±2.19 mg AAE/g extract). In ABTS radical scavenging assay, aqueous leaf extract (12.11±0.07 mg trolox equivalent (TE)/g extract) showed the best response. The effective ferric reducing antioxidant property (141.54±10.12 mg Fe (II)/g extract) was exhibited by aqueous fruit extract. Overall, methanol and water were found to be the best solvents for the extraction of antioxidant compounds from fruit and leaf. In the RP-HPLC analysis, the major bioactive phenolic compounds such as catechin (CA) and hydroxybenzoic acid (HBA) were recorded in leaf as compared to fruit. In leaf, CA and chlorogenic acid (CLA) were principal compounds in leaf and fruit respectively. However, gallic acid (GA), HBA, CLA and vanillic acid (VA) were widespread in leaf and fruit. Conclusion: On the basis of the results, it was found that D. palmatus may serve as a novel and rich source of natural antioxidants and it can be further explored for pharmaceutical purposes.

scholarly journals Free radical scavenging activity, total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts

2012 ◽  
Vol 66 (4) ◽  
pp. 547-552 ◽  
Author(s):  
Marija Radojkovic ◽  
Zoran Zekovic ◽  
Senka Vidovic ◽  
Drago Kocar ◽  
Pavle Maskovic
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Phenolic Components ◽  
Dpph Method ◽  
Morus Spp ◽  
Phenolic And Flavonoid

Mulberry (Morus spp. L., Moraceae) fruits, leaves, bark and branch have been used in traditional medicine as diuretic, hypoglycemic and hypotensive. The mechanism of their effects is correlated with the content of active components. Objective of this work was to evaluate and compare antioxidant properties of different extracts of two Morus species growing in Serbia: Morus alba L. (white mulberry) and Morus nigra L. (black mulberry). Potential antioxidant activity, content of antioxidant compounds (phenolics and flavonoids) and radical scavenging capacity, tested by DPPH method, were evaluated. The phenolic and flavonoid composition of different Morus extracts was determined by the HPLC method. The extracts prepared from fruits, leaves and roots of M. alba and M. nigra exhibited different characteristics. The highest extraction yield was achieved by M. alba leaves extraction (23.40%). M. nigra roots extract shown the highest total phenolics (186.30 mg CAE/g), while highest total flavonoids content (67.37 mg RE/g) was determined for M. nigra leaves extracts. In addition, black mulberry leaves extracts with the highest antioxidant activity had the highest phenolic acids contents. The dominant phenolic components in the samples were rutin and chlorogenic acid. All investigated mulberry dry extracts shown high content of phenolic compounds and significant antioxidant activity. This work contributes to knowledge of the antioxidant properties of Morus species. The obtained results may be useful in the evaluation of new dietary supplements and food products.

scholarly journals Biocompatibility and Antioxidant Capabilities of Carbon Dots Obtained from Tomato (Solanum lycopersicum)

2022 ◽  
Vol 12 (2) ◽  
pp. 773
Author(s):  
Sandra Rodríguez-Varillas ◽  
Tania Fontanil ◽  
Álvaro J. Obaya ◽  
Alfonso Fernández-González ◽  
Clarissa Murru ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Dermal Fibroblast ◽  
Antioxidant Properties ◽  
Carbon Sources ◽  
Natural Antioxidants ◽  
Human Dermal Fibroblast ◽  
Tomato Juice ◽  
Antioxidant Power ◽  
Lung Carcinoma Cells ◽  
Dpph Method

Since their discovery in 2004, carbon dots have attracted strong interest in the scientific community due to their characteristic properties, particularly their luminescence and their ease of synthesis and derivatization. Carbon dots can be obtained from different carbon sources, including natural products, resulting in a so-called ’green synthesis’. In this work, we obtain carbon dots from tomato juice in order to obtain nanoparticles with the antioxidant capabilities of the natural antioxidants present in that fruit. The obtained material is characterized regarding nanoparticle size distribution, morphology, surface functional groups and optic properties. Antioxidant properties are also evaluated through the DPPH method and their cytotoxicity is checked against human dermal fibroblast and A549 cell-lines. The results indicate that carbon dots obtained from tomato have a higher antioxidant power than other already-published antioxidant carbon dots. The bandgap of the synthesized materials was also estimated and coherent with the literature values. Moreover, carbon dots obtained from tomato juice are barely toxic for healthy cells up to 72 h, while they induce a certain cytotoxicity in A549 lung carcinoma cells.

scholarly journals ХИМИЧЕСКИЙ СОСТАВ И АНТИОКСИДАНТНЫЕ СОЕДИНЕНИЯ МУЧНОЙ КОМПОЗИТНОЙ СМЕСИ С ДОБАВЛЕНИЕМ БРУСНИКИ И ПАЖИТНИКА

2019 ◽  
Author(s):  
L.V. Anisimova ◽  
O.I.A. Soltan
Keyword(s):  
Chemical Composition ◽  
Vitamin C ◽  
Wheat Gluten ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Raw Material ◽  
Antioxidant Compounds ◽  
Fenugreek Seed ◽  
Composite Mixture ◽  
Oat Flour

The effect of fenugreek and lingonberry supplements, containing natural antioxidants and a lot of important nutrition components, on the chemical composition and antioxidant content of flour mixture based on whole oat flour were investigated. Oat flour was produced using hydrothermal treatment, which included intensive moistening of the grain in the vacuum unit, standing, and drying. In addition to whole oat flour, the highgrade wheat flour, dry wheat gluten, and fenugreek seed powder or dried lingonberry powder were added to the flour composite mixture. The chemical composition of raw material and flour composite such as protein, carbohydrates, fat, and moisture was determined. Besides, antioxidant components, like Vitamin C and flavonoids in raw material and flour composites were investigated. From results, it has been established that adding fenugreek seed powder and dried lingonberry powder to flour composite based on oat flour leads to significant enhancing in the chemical composition and antioxidant compounds: the addition of the fenugreek seed powder led to the increase of protein content in the flour composite. Furthermore, adding dried lingonberry powder and fenugreek seed powder led to a slight effect on carbohydrates. The addition of fenugreek seed powder has the greatest effect on the content of flavonoids. In addition, vitamin C was significantly increased by the addition of lingonberry. According to, the results of our research can recommend the addition of fenugreek seed powder and lingonberry to oatwheat flour composite leading to improvement of its nutritional and antioxidant properties.

scholarly journals POTENSI ANTIOKSIDAN ALAMI PADA EKSTRAK DAUN JAMBLANG (Syzigium cumini (L.) Skeels)

2017 ◽  
Vol 18 (02) ◽  
pp. 107-112
Author(s):  
Ayu Nirmala Sari
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Leaf Extract ◽  
Natural Antioxidants ◽  
The Body ◽  
Antioxidant Potential ◽  
Negative Effects ◽  
Antioxidant Content ◽  
Dpph Method ◽  
In Cells

Various diseases can be triggered by the condition of oxidative stress in the body. Oxidative stress is a condition that occurs in cells when free radicals are present in excess amounts. To reduce the negative effects and negative effects of these free radicals, the body needs antioxidants. The aim of this research is to know the antioxidant potential of black plum leaf extract (Syzigium cumini (L.) Skeel) using DPPH method. Based on the results of antioxidant potential testing in black plum leaf extract known that it has antioxidant content that is classified as very active with an IC 50 value of 8.85. The results of this study indicate that the leaves of black plum (Syzigium cumini (L.) Skeel have the potential to be developed as a source of natural antioxidants for humans.

scholarly journals Biomarkers and Utility of the Antioxidant Potential of Probiotic Lactobacilli and Bifidobacteria as Representatives of the Human Gut Microbiota

Biomedicines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1340
Author(s):  
Olga V. Averina ◽  
Elena U. Poluektova ◽  
Mariya V. Marsova ◽  
Valery N. Danilenko
Keyword(s):  
Oxidative Stress ◽  
Gut Microbiota ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Antioxidant Potential ◽  
Diagnostic Systems ◽  
Human Gut ◽  
Human Gut Microbiota

Lactobacilli and bifidobacteria are an important part of human gut microbiota. Among numerous benefits, their antioxidant properties are attracting more and more attention. Multiple in vivo and in vitro studies have demonstrated that lactobacilli and bifidobacteria, along with their cellular components, possess excellent antioxidant capacity, which provides a certain degree of protection to the human body against diseases associated with oxidative stress. Recently, lactobacilli and bifidobacteria have begun to be considered as a new source of natural antioxidants. This review summarizes the current state of research on various antioxidant properties of lactobacilli and bifidobacteria. Special emphasis is given to the mechanisms of antioxidant activity of these bacteria in the human gut microbiota, which involve bacterial cell components and metabolites. This review is also dedicated to the genes involved in the antioxidant properties of lactobacilli and bifidobacteria strains as indicators of their antioxidant potential in human gut microbiota. Identification of the antioxidant biomarkers of the gut microbiota is of great importance both for creating diagnostic systems for assessing oxidative stress and for choosing strategies aimed at restoring the normal functioning of the microbiota and, through it, restoring human health. In this review, the practical application of probiotic strains with proven antioxidant properties to prevent oxidative stress is also considered.

Sign in / Sign up

Export Citation Format

Share Document