scholarly journals Utilization of Eco-Enzymes from Fruit Skin Waste as Hand Sanitizer

2021 ◽  
Vol 5 (3) ◽  
Author(s):  
Rusdianasari Rusdianasari ◽  
Adi Syakdani ◽  
Muhammad Zaman ◽  
Muhammad Zaman ◽  
Febby Fitria Sari ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Organic Waste ◽  
Fruits And Vegetables ◽  
Ph Value ◽  
Screening Tests ◽  
Alcohol Content ◽  
Brown Sugar ◽  
Fermentation Time ◽  
Hand Sanitizer ◽  
Fruit Skin

Eco-enzyme is a multifunctional liquid produced from fermentation of waste or organic waste, brown sugar or granulated sugar, and water. Fermentation of  eco-enzymes  is carried out for ±3 months. The length of time fermentation affects color, aroma and pH. The  resultingeco-enzyme liquid is dark brown and has a strong fresh sour aroma.  Eco-enzymeliquid has an alcohol content and acetic acid. Alcohol and acetic acid are produced from the bacterial metabolic process that is naturally present in the rest of fruits and vegetables. Variations in the fermentation time of eco-enzymes  used are 2.5 months and 3 months. Analysis conducted on  eco-enzymes  include pH tests and phytochemical screening  tests. The pH value obtained in  eco-eznyme  2.5 months is 3, while the pH value in  eco-eznyme  3 months is 1.5. Eco-enzymes  with a fermentation time of 3 months contain alkaloid compounds, flavonoids, saponins, and tannins. In this study  Eco-eznyme  was used as a hand sanitizer  spray by diluting the eco-enzyme with aquadest. The dilution ratios used vary for each sample: 1:40, 2:40, 3:40, 4:40, 5:40. Analysis conducted on  hand sanitizer spray include pH test, organoleptic test, and antibacterial activity test. Based on the results of the analysis, not all hand sanitizer samples meet SNI standards, the best samples obtained in hand sanitizer with an eco-enzyme fermentation time of 3 months and a dilution ratio of 5: 40. The results of the analysis showed that the value of pH 5, the level of aroma fondness in hand sanitizer 2.40, the favorite color level 2.46

scholarly journals Acceleration of Production Natural Disinfectants from the Combination of Eco-Enzyme Domestic Organic Waste and Frangipani Flowers (Plumeria alba)

2021 ◽  
Vol 5 (1) ◽  
pp. 15-21
Author(s):  
Made Rai Rahayu ◽  
Muliarta Nengah ◽  
Yohanes Parlindungan Situmeang
Keyword(s):  
Organic Waste ◽  
Ph Value ◽  
Raw Materials ◽  
Alcohol Concentration ◽  
Alcohol Content ◽  
Fixed Amount ◽  
Brown Sugar ◽  
Fermentation Time ◽  
Water Composition ◽  
Fruit Skin

Spraying disinfectants is one of the efforts to prevent the transmission of COVID-19. One of the high needs for disinfectants can be overcome by using natural disinfectants or those made from natural ingredients, such as the use of eco-enzymes. The problem is that it takes 3 months to produce eco-enzyme, so it needs innovation to be able to produce it quickly. This research is an experimental study with independent variables, namely the composition of organic waste (rambutan fruit skin, corn cobs, chayote skin) as a substrate in varied eco-enzyme raw materials. Each treatment added 10% frangipani sandalwood extract. Control variables include fermentation time, water composition, brown sugar, yeast (Saccharomyches cerevisiae) with a fixed amount. The experimental results showed that the longer of fermentation time, the alcohol concentration increased, while the pH value decreased. The optimum time to produce eco-enzymes according to standard requirements for disinfecting purposes is 8-10 days, wherein the fermentation has produced an alcohol content of 60-70% and the pH of eco-enzymes has been reached below 4.0. The content of compounds in frangipani flower extract through phytochemical and GC-MS tests include terpenoids (linalool, geraniol, terpineol), quercetin from the flavonoid and citrulline groups of alkaloids and tannins which have various activities that support their role as a natural disinfectant including antibacterial, anti-fungi, and antivirals. The combination of Eco-Enzym from domestic organic waste and frangipani flower (Plumeria alba) is able to inhibit the growth of Staphylococcus aureus bacteria with very strong inhibition category ranging from 31.85-34.41 mm.   Keywords: disinfectant, eco-enzyme, frangipani flowers

scholarly journals Pemanfaatan Limbah Padat Sari Apel sebagai Bahan Baku Cuka Apel Menggunakan Metode Backslop

2019 ◽  
Vol 2 (1) ◽  
pp. 136
Author(s):  
Alvianty Novitasari ◽  
Warkoyo Warkoyo ◽  
Sri Winarsih
Keyword(s):  
Acetic Acid ◽  
Phase Ii ◽  
Fermentation Process ◽  
Ph Value ◽  
Raw Material ◽  
Soluble Solids ◽  
Acetobacter Aceti ◽  
Alcohol Content ◽  
Apple Cider ◽  
Factor I

Solid wasted of apple cider containing a lot of compounds such as carbohydrates, glucose, malic acid, and flavonoids. The purpose of this research is to utilize the solid wasted of apple cider as the raw material of apple vinegar. The fermentation process of making apple vinegar in this research using the backstop method. This research consists of 2 steps of the fermentation process. The first step of fermentation using yeast to transform sugar into alcohol. Second step fermentation is a continuance of first step fermentation with the addition of apple vinegar backstop culture which contain Acetobacter aceti with density 4 x 107cfu / ml to transform alcohol to acetic acid. This research uses simple and factorial Randomized Block Design (RBD). Fermentation phase I using simple RBD with the proportion of apple raw material (solid wasted of apple cider: apple) 100% : 0%; 75%: 25%; 50%: 50%; 25%: 75% as factor I. Fermentation phase II using factorial RBD with the combination of factor I and the addition of apple vinegar backstop with concentration 5%, 10%, and 15% as factor II. The results showed that during the first step fermentation process the raw material proportion of apple (solid wasted of apple cider: apple) affected total soluble solids, pH value, and alcohol content. Fermentation phase II showed an interaction between the proportion of the raw material of apple (apple cider waste: apple) and the addition of backstop apple vinegar concentration to total dissolved solids, alcohol content, acetic acid, except pH value. The best results showed treatment with apple material proportion (25% solid wasted of apple cider: 75% apple) and addition of apple vinegar backstop concentration 15 % produce 4.6 g / 100ml acetic acid, 4% soluble solids total, pH value of 3.4 and alcohol residue of 0% (v/v), with colorful organoleptic results quite appealing, the scent is sufficient, and preferences are favored by the panelists.

scholarly journals Pengaruh Penambahan Ragi Tape dan Waktu Fermentasi Hasil Samping Cairan Pulpa terhadap Karakteristik Mutu Cuka Kakao (Theobroma cacao L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 177
Author(s):  
Ida Bagus Gede Awidyanata ◽  
G.P Ganda Putra ◽  
Luh Putu Wrasiati
Keyword(s):  
Acetic Acid ◽  
Block Design ◽  
Total Dissolved Solids ◽  
Total Sugar ◽  
Alcohol Content ◽  
Dissolved Solids ◽  
Fermentation Time ◽  
Natural Fermentation ◽  
Acid Ph ◽  
Cocoa Bean Fermentation

The aim of this study was to determine the effect of adding ragi tape and the time of natural fermentation of the pulp byproducts of the fermented cocoa beans on the characteristics of the quality of cocoa vinegar and determining the addition of tape yeast and the natural fermentation time of the best pulp fluid for cocoa vinegar production. The experiments in this study used a randomized block design with two factors. The first factor is the addition of yeast tape consisting of 5 levels, namely without the addition of yeast tape (control), the addition of yeast tape 0.05% (w / v), 0.10% (w / v), 0.15% (w / v), 0.20% (w / v), the second factor is the fermentation time consisting of 6 levels, namely 5, 10, 15, 20, 25, 30 days. Treatment The addition of tape yeast has a very significant effect on acetic acid, pH, total dissolved solids, total sugar, has no significant effect on alcohol content in the fermented vinegar from the pulp fluid which is the by-product of cocoa bean fermentation. The length of fermentation treatment has a very significant effect on acetic acid, pH, total dissolved solids, total sugar, alcohol. The interaction between the two treatments had no significant effect on acetic acid, pH, total dissolved solids, alcohol content, and significant effect on total sugar in fermented cocoa vinegar products. The best treatment was obtained from the addition of 0.15% (w / v) yeast tape and 30 days fermentation time which produced cacao vinegar with acetic acid content of 2.40%, pH 3.37, total dissolved solids 4.25 obrix, total sugar 0.03%, and 0.00% alcohol. Keywords  : vinegar fermentasion, ragi tape, time of fermentation

Peran Akar Kayu Bayur (Pterospermum sp.) Terhadap Fermentasi Nira Aren (Arenga pinnata)

2019 ◽  
Vol 20 (1) ◽  
pp. 81
Author(s):  
Siti Nurlina Mentari ◽  
Muhammad Jasri Djangi ◽  
Sudding Sudding
Keyword(s):  
Acetic Acid ◽  
Ph Value ◽  
Alcohol Content

ABSTRAK Penelitian ini bertujuan untuk mengetahui peran akar kayu bayur terhadap fermentasi nira aren dengan parameter pengukuran yaitu derajat keasaman (pH) dan kadar alkohol. Peran akar kayu bayur (Pterospermum sp.) dapat dilihat dari nilai pH dan kadar alkohol hasil fermentasi nira aren tanpa penambahan akar kayu bayur dengan nira aren menggunakan akar kayu bayur selama proses fermentasi 72 jam. Hasil penelitian menunjukkan bahwa akar kayu bayur yang ditambahkan pada nira aren berfungsi sebagai pengawet tuak sehingga menyebabkan kadar alkohol hasil fermentasi nira aren bertahan lebih lama pada konsentrasi 4% selama 64 jam serta memperlambat fermentasi alkohol menjadi asam asetat. Kata kunci: Nira aren (Arenga pinnta), Akar kayu bayur (Pterospermum sp), Fermentasi ABSTRACT This research aimed to determine the role of wood root bayur toward the fermentation of sugarpalm nira with measuring parameters, i.e, the degree of acidity (pH) and alcohol content. Role of wood root bayur (Pterospermum sp.) was observed from the pH value and the alcohol content of fermented sugarpalm nira without the addition of wood root bayur compared to the fermented sugarpalm nira using wood root bayur during the 72 hours of fermentation. The results showed that the wood root bayur added to the sugarpalm nira served as nira preservatives, causing the alcohol content of fermented sugarpalm nira last longer at the concentration of 4% for 64 hours and retarding the fermentation of alcohol into acetic acid. Keywords: Sugarpalm nira (Arenga pinnata), wood root bayur (Pterospermum sp.), fermentation

Formulasi sediaan Cuka Buah Kopi Menggunakan Ragi (Saccharomyces cerevisiae) dan Bakteri (Acetobacter aceti)

2021 ◽  
Vol 1 (1) ◽  
pp. 38-45
Author(s):  
Fathan Said R ◽  
Gita Cahya Eka Darma ◽  
Reza Abdul Kodir
Keyword(s):  
Acetic Acid ◽  
Fruits And Vegetables ◽  
Sweet Taste ◽  
The Body ◽  
Test Results ◽  
Acetobacter Aceti ◽  
Alcohol Fermentation ◽  
Alcohol Content ◽  
Coffee Pulp ◽  
Coffee Pulp Waste

Abstract. Indonesia is the third largest coffee producing country in the world, with a variety of compounds that are beneficial to the body. Vinegar fermentation is one way to add value to the benefits of fruits and vegetables because it can form useful new chemical compounds. Vinegar has a variety of benefits that have been studied such as reducing hyperglycemia, hyperinsulinemia, hyperlipidemia and obesity. Previous research in 2013 had made acetic acid from Arabica coffee pulp waste. This study aims to obtain a coffee fruit vinegar formula that conforms to the quality standards of acetic acid SNI 01-4371-1996. The material used is whole coffee with a two-stage fermentation method, namely alcohol fermentation using S. cerevisiae for 4 days and vinegar fermentation using A. aceti for 3 days. The results showed that the formula with 25% coffee fruit and 20% sugar is the best formula compared to other formulas with organoleptic test results in brown, sour and sweet taste and a little distinctive aroma of coffee, 5.03% acetic acid content, 0% alcohol content, pH 3,242. Abstrak. Indonesia merupakan negara ketiga penghasil kopi terbesar di dunia, dengan berbagai kandungan senyawa yang bermanfaat bagi tubuh. Fermentasi cuka merupakan salah satu cara untuk menambah nilai manfaat dari buah dan sayur karena dapat membentuk senyawa kimia baru yang bermanfaat. Cuka memiliki berbagai manfaat yang telah diteliti seperti menurunkan hiperglikemia, hiperinsulinemia, hiperlipidemia dan obesitas. Penelitian sebelumnya pada tahun 2013 telah dilakukan pembuatan asam asetat dari limbah cair kulit kopi arabika. Penelitian ini bertujuan untuk mendapatkan formula cuka buah kopi yang sesuai standar mutu asam asetat SNI 01-4371-1996. Bahan yang digunakan adalah buah kopi secara utuh dengan metode fermentasi dua tahap yaitu fermentasi alkohol menggunakan S. cerevisiae selama 4 hari dan fermentasi cuka menggunakan         A. aceti selama 3 hari. Hasil penelitian menunjukan bahwa formula dengan 25% buah kopi dan 20% gula merupakan formula terbaik dibandingkan dengan formula lain dengan hasil uji organoleptis berwarna coklat, rasa asam dan manis serta sedikit aroma khas kopi, kadar asam asetat 5,053%, kadar alkohol 0%, pH 3,242.

PENGEMBANGAN MINUMAN BERBASIS TEH DAN REMPAH SEBAGAI MINUMAN FUNGSIONAL

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Siti Chairiyah Batubara ◽  
Nindia Arum Pratiwi
Keyword(s):  
Ph Value ◽  
Raw Materials ◽  
Mixture Design ◽  
Total Sugar ◽  
Brown Sugar ◽  
Physical Test ◽  
Chemical Test ◽  
Carbohydrate Level ◽  
Optimum Formula ◽  
Functional Drink

Teh dan rempah memiliki kandungan antioksidan yang berguna untuk kesehatan. Tujuan dari pembelajaran ini adalah untuk mendapatkan formula minuman fungsional teh dan rempah terbaik. Penelitian ini menggunakan mixture design untuk menentukan formula optimum minuman fungsional terbaik yang terdiri dari gula merah, bubuk kayu manis dan bubuk kapulaga. Berdasarkan input batas atas dan batas bawah dari bahan baku sehingga didapatkan 16 formulasi yang akan diteliti. Untuk batas atas gula merah yaitu 40 dan batas bawah gula merah yaitu 35. Untuk batas atas bubuk kayu manis yaitu 15 dan batas bawah kayu manis yaitu 10. Untuk batas atas bubuk kapulaga yaitu 50 dan batas bawah bubuk kapulaga yaitu 45. Untuk menentukan kualitas minuman fungsional dilakukan uji kimia (uji karbohidrat, gula total dan nilai pH, uji fisik (Uji viskositas), uji organoleptik (uji hedonik untuk warna, aroma, rasa dan kekentalan) dan mutu hedonik yang ditentukan dari kesukaan panelis terhadap warna, aroma, rasa dan kekentalan) dan uji antioksidan. Penelitian ini untuk mendapatkan formula optimum yang terbaik dari hasil pengacakan oleh mixture design. Hasil dari pengolahan mixture design adalah anova, grafik dan duncan. Berdasarkan hasil penelitian formula minuman fungsional yang masih dapat diterima adalah formulasi 11 yang terdiri dari 40% gula merah, 12.67% bubuk kayu manis dan 47.32% bubuk kapulaga. Formula 11 mengandung 0.39% karbohidrat, 15.46% gula total, 5.38% pH 4.98%  viskositas dan 255.41 mg antoksidanSpice tea have antioxidants compounds that are beneficial for health. The purpose of this study was to get the best spice tea formulation as a functional drink. This research used a mixture design to obtain the best optimum formula functional drink that composed by brown sugar, cinnamon powder and cardamom powder. Based on input the upper and lower limits of raw materials then get 16 formulations will be researched. The upper limit of brown sugar, cinnamon powder and cardamom powder is 40, 15 and 50. The lower limit of brown sugar, cinnamon powder and cardamom powder is 35, 10 and 45. The functional drink quality determined by chemical test (carbohydrate level, total sugar and pH value), physical test (viscosity level), organoleptic test and antioxidan level. Data was processed statistically using Design Expert application with one-way analysis (one way ANOVA) at 95% confidence level. Duncan`s follow-up was carried out to find out the differences between treatments if ANOVA had a significant effect. Based on the results of the research, formulation functional drink are still aceptable is formulation 11 of 40% brown sugar, 12.67% cinnamon powder and 47.32% cardamom powder. Formulation 11 contained carbohydrate level 0.39%, total sugar 15.46% and pH value 5.38%, viscosity level 4.98%, and antioxidan level 255.41 mg

scholarly journals Changes in folate characteristics and its identification in broccoli (Brassica oleracea Italica) extract fermented by Lactic Acid Bacteria Mixed Culture (LAB)

2018 ◽  
Vol 154 ◽  
pp. 04001 ◽  
Author(s):  
Yati Maryati ◽  
Agustine Susilowati
Keyword(s):  
Lactic Acid ◽  
Folic Acid ◽  
Lactic Acid Bacteria ◽  
Brassica Oleracea ◽  
Acid Concentration ◽  
Ph Value ◽  
Starter Culture ◽  
Time Interval ◽  
Fermentation Time ◽  
Total Sugars

Broccoli (Brassica oleracea Italica) was fermented by cultures of lactic acid bacteria (LAB) as a potential source of natural folic acid. This study aimed to evalte characteristic changes and to identify folate compounds from broccoli extract, fermented by mixed LAB cultures (L. bulgaricus, S. thermophulus, L.acidophilus, Bd. bifidum). The formulation of broccoli extract was fermented with variation of LAB starter culture with concentrations of 10 and 20%(v/v), and the change of characteristic of folic acid compound during fermentation (0 to 48 hours) with an interval of 8 hours was evaluated. The results showed that the fermentation of broccoli extract with different concentration of LAB culture had an effect on the concentration of folic acid produced, as well as the change of concentration of folic acid during the fermentation time interval. The optimum condition was obtained based on the highest folic acid concentration of 6.74%, at culture concentration of 20% during 24 hour fermentation with the value of folic acid concentration of 72.11 μg/mL, pH value of 4.29, total sugars of 34.61%, total acids of 0, 97%, dissolved protein of 14.64 mg/mL and total LAB of log 13.02 + 0.05 cfu / ml.

GROWTH OF STAPHYLOCOCCUS AUREUS IN ACIDIFIED PASTEURIZED MILK1

1970 ◽  
Vol 33 (11) ◽  
pp. 516-520 ◽  
Author(s):  
T. E. Minor ◽  
E. H. Marth
Keyword(s):  
Staphylococcus Aureus ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Hydrochloric Acid ◽  
Ph Value ◽  
The Other ◽  
Antagonistic Effects ◽  
Pasteurized Milk ◽  
Ph Values ◽  
Effect On Growth

The effect of gradually reducing the pH of pasteurized milk with acetic, citric, hydrochloric, lactic, and phosphoric acids over periods of 4, 8, and 12 hr on growth of Staphylococcus aureus 100 in this substrate was determined. In addition, 1: 1 mixtures of lactic acid and each of the other acids, and of acetic and citric acids were evaluated for their effect on growth of this organism. To achieve a 90% reduction in growth over a 12 hr period, a final pH value of 5.2 was required for acetic, 4.9 for lactic, 4.7 for phosphoric and citric, and 4.6 for hydrochloric acid. A 99% reduction during a 12 hr period was obtained with a final pH value of 5.0 for acetic, 4.6 for lactic, 4.5 for citric, 4.1 for phosphoric, and 4.0 for hydrochloric acid. A pH value of 3.3 was required for a 99.9% reduction with hydrochloric acid, whereas the same effect was produced at a pH value of 4.9 with acetic acid. Correspondingly lower pH values were required to inhibit growth within 8 and 4 hr periods. Mixtures of acids adjusted to pH values at the borderline for growth (12 hr period) exhibited neither synergistic nor antagonistic effects between two acids.

scholarly journals STUDI PEMANFAATAN KULIT KOPI ARABIKA (Coffee arabica L.) SEBAGAI MIKRO ORGANISME LOKAL (MOL)

AGROINTEK ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 1
Author(s):  
A Ita Juwita ◽  
Arnida Mustafa ◽  
Risna Tamrin
Keyword(s):  
Food Source ◽  
Material Decomposition ◽  
Brown Sugar ◽  
Fermentation Time ◽  
Fermentation Method ◽  
Local Material ◽  
Coffee Arabica ◽  
Phosphorus And Potassium ◽  
Macro Nutrients

<span class="fontstyle0">MOL (Mikro Organisme Lokal) is the result of local material decomposition with<br />fermentation method. MOL contains macro and micro nutrients and microbial decomposers.<br />The smaller coffee peel that will fermented, the faster MOL can be formed. To accelerate<br />decomposition in MOL solution, it can be added food source of bacteria such as coconut water<br />and brown sugar.<br />The purpose of this study was to study the process of making MOL from coffee peel, to<br />analyze the content of nitrogen, phosphor, and potassium of MOL of coffee peel and to<br />determine the effect of fermentation time on nitrogen, phosphor, and potassium contents of<br />MOL that resulted.<br />This study was conducted with 4 (four) treatments, week 1, 2, 3 and 4 and 1 factor and<br />using 2 times replicate. The parameter of testing used for making coffee peel as local<br />microorganism are MOL volume, nitrogen, phosphor and potassium contents.<br />The result of study showed that the highest mol volume obtained in week 4 of 8.5 ml and<br />the lowest in week 1 of 5 ml. They were obtained from 500 g coffee peel. Determination of<br />nitogen, phosphorus and potassium as a macro nutrients has been conducted. The result showed<br />that the highest nitrogen was found to be 0,0039% in week 1, 3 and 4 and the lowest in week 2<br />with percentage 0.034 %. Determine of phosphorus showed that the highest phosphor was<br />obtained in week 2 wtih percentage 0.033 % and the lowest in week 4 of 0.018%, and the result<br />of potassium analyze, the highest obtained in week 2 of 0.035 % and the lowest in week 4 of<br />0.014%</span>

scholarly journals Kajian Mutu Wine Coffee Arabika Gayo

2018 ◽  
Vol 3 (4) ◽  
pp. 822-829
Author(s):  
Achmad Dairobbi ◽  
Irfan Irfan ◽  
Ismail Sulaiman
Keyword(s):  
Sampling Method ◽  
Sugar Content ◽  
Complex Compounds ◽  
Ash Content ◽  
Closed Space ◽  
Alcohol Content ◽  
Fermentation Time ◽  
Purposive Sampling ◽  
High Sugar Content

Buah kopi memiliki kandungan gula tinggi yang dapat diproses dengan cara fermentasi alami. Fermentasi kopi arabika bertujuan untuk mengurangi rasa pahit dan meningkatkan citarasa kopi. Senyawa-senyawa kompleks pada kopi fermentasi akan meningkatkan mutu kopi. Penelitian ini menggunakan metode survey purposive sampling, yaitu cara pengambilan sampel berdasarkan pertimbangan tertentu dan disesuaikan dengan ketersediaan produsen wine coffee. kadar air wine coffee rata-rata 9.08% (SNI), kadar abu rata-rata 4.5% (SNI) dan kadar alkohol 0%. Dari 6 sampel wine coffee yang di analisis, total skor terbaik uji deskriptif ditunjukkan pada sampel E yaitu 83,75 dan sampel F yaitu 83,00. Secara keseluruhan, 6 sampel wine coffee di Kabupaten Aceh Tengah terdapat perbedaan pada lamanya waktu fermentasi wine coffee yang dibutuhkan. Hal ini dibuktikan pada sampel E yaitu proses pembuatan wine coffee dilakukan dengan waktu fermentasi 7-10 hari pada suhu secara natural di dalam ruang tertutup. Abstract: Coffee fruit has a high sugar content that can be processed by natural fermentation. Fermented arabica coffee aims to reduce bitterness and improve coffee flavor. The complex compounds in fermented coffee will improve the quality of coffee. This research uses survey purposive sampling method, that is the way of sampling based on certain consideration and adjusted with the availability of wine coffee producer. Water wine coffee average 9.08% (SNI), average ash content of 4.5% (SNI) and alcohol content 0%. From 6 samples of analyzed wine coffee, the best total score of descriptive test is shown on sample E that is 83,75 and sample F is 83,00. Overall, 6 samples of wine coffee in Aceh Tengah District have differences in the duration of fermentation of the required coffee. This is evidenced in the sample E is the process of making wine coffee is done with a fermentation time of 7-10 days at a temperature naturally in a closed space.

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