Gambaran Makroskopis Persembuhan Luka Mukosa Buccal Tikus Putih (Rattus norvegicus) Pascapemberian Policresulen

This study aimed to investigate the effect of policresulen treatment with various concentrations on the wound healing in buccal mucosa of white rats (Rattus norvegicus) through macroscopically observation. The number of 54 rats were divided into six treatment groups policresulen with concentration of 36%, 10%, 5%, 1%, 0.3% and one control group. The buccal mucosal injury was made with a 0.5 cm biopsy punch. The treatment of mucosal injury was conducted by dropped policresulen on the 2nd day until the 5th day after the injury. Macroscopic observation under a stereo micro-scope was conducted on the wound diameter, redness, swelling, and necrose. The results showed that wound healing was influenced significantly with concentration of the policresulen. The best healing was shown on lowest concentration marked which has lowest size of the wound diameter (0.50 ± 0.00 cm), the lowest tissue redness (0.00 ± 0.00), lowest final swelling at a concentration of 0.3%. Clinical evaluation of wound tissue necrose parameters which were seen to be most severe at 10% policresulen administration followed by 36% concentration and the least necrose shown on policresulen administration 0.3%. Therefore, based on macroscopic images with parameters of wound diameter, redness, swelling, and necrose suggested that policresulen used for wound healing process are at a concentration of 0.3%.

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FGF-2 expression and the amount of fibroblast in the incised wounds of Rattus norvegicus rats induced with Mauli banana (Musa acuminata) stem extract

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v50.i3.p121-126 ◽

2018 ◽

Vol 50 (3) ◽

pp. 121

Author(s):

Didit Aspriyanto ◽

Intan Nirwana ◽

Hendrik Setia Budi

Keyword(s):

Wound Healing ◽

Rattus Norvegicus ◽

Healing Process ◽

Control Group ◽

Wound Healing Process ◽

Fibroblast Cells ◽

Treatment Groups ◽

Incised Wound ◽

Stem Extract ◽

Banana Stem

Background: Traditional wound treatment using herbal medicine is thought to maintain the health of families and society in general economically, effectively, and efficiently without inducing side effects. One genus of plant that can be used as a traditional medicine is the Mauli banana, indigenous to South Borneo. Mauli banana stem contains bioactive compounds, most of which are tannins along with ascorbic acid, saponin, β-carotene, flavonoids, lycopene, alkaloids, and flavonoids. Tanin has antibacterial and antioxidant effects at low concentrations, as wells as antifungal ones at high concentrations. Purpose: This study aimed to analyze the effects of Mauli banana stem extract at concentrations of 25%, 37.5%, and 50% on the quality of incised wound healing in male Rattus norvegicus rats by assessing FGF-2 expression and fibroblast concentration on days 3 and 7. Methods: This research represented an experimental laboratory-based investigation involving 32 rats of the Rattus norvegicus strain aged 2-2.5 months old. Sampling was performed using a simple random sampling technique since the research population was considered homogeneous and divided into 8 treatment groups (C3, M3-25, M3-37.5, M3-50, C7, M7-25, M7-37.5, M7-50). The rats in each group were anesthetized before their back was incised with length and width of 15x15mm with a depth of 2mm. Gel hydroxy propyl cellulose medium (HPMC) was applied to the incised wound of each rat in the control group, while stem Mauli banana extract was applied to that of each rat in the treatment groups three times a day at an interval of 6-8 hours. On day 3, four rats from each group were sacrificed, while, in the remaining groups, the same procedure was performed until day 7, at which point they (8 groups) were sacrificed for HE examination in order to assess the amount of fibroblast and for IHC examination to examine FGF-2 expression. Data regarding FGF-2 expression and the amount of fibroblast were analysed by means of One-way Anova and HSD. Results: The results showed that the Mauli banana stem extract could significantly improve the expression of FGF-2 and the amount of fibroblast cells compared to C3 and C7 groups. The highest increase in FGF-2 expression and fibroblast amount were found in all groups on days 3 and 7 treated with the Mauli banana stem extract at a concentration of 50%. Conclusion: There was an increase of FGF-2 expression and the amount of fibroblast cells in the incision wound healing process that induced with Mauli banana stem extract.

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Althaea officinalis extracts heal skin wounds in second- and third-degree burns in mice

10.21203/rs.3.rs-145872/v1 ◽

2021 ◽

Author(s):

Ali Mohammad Ranjbar ◽

Arefeh Sojaeemehr ◽

Mohsen Zabihi ◽

Fatemeh Shishehbor ◽

Vahid Ramezani

Keyword(s):

Wound Healing ◽

Healing Process ◽

Metal Plate ◽

Control Group ◽

Wound Healing Process ◽

Aqueous Extracts ◽

Treatment Groups ◽

Iranian Traditional Medicine ◽

Althaea Officinalis ◽

Skin Healing

Abstract Background and Purpose: Burns are one of the most common injuries. There are few medications to repair skin in burns. Although antibacterial drugs such as topical silver sulfadiazine may prevent wound infections, they usually can’t attenuate skin healing. Marshmallow is used in Iranian traditional medicine to improve skin burns. We aimed to evaluate the effect of hydroalcoholic and aqueous extract of Althaea officinalis on the histopathologic parameters of second- and third-degree burns in mice. Experimental approach: The mice were randomly divided into 9 groups (n=6) including control and treatment groups. Experimental second- and third-degree burns were induced on dorsal skin of the mice by a metal plate (2 cm in diameter) under general anesthesia. Control and treatment groups were treated twice daily for 21 days topically. Normal saline was used for control group and hydroalcoholic and aqueous extracts of Althaea officinalis in three concentrations (1%, 2% and 4%) were used for the treatment groups. All of the preparations were made in hydrogel base. Finally, the histopathological parameters of the burned skin including polymorphonuclear (PMN) migration, epithelialization, angiogenesis and collagenization were scored and total score of wound healing was calculated. Key Results: The findings showed that topical application of both hydroalcoholic and aqueous extracts of Althaea officinalis attenuates the wound healing process. they reduce PMN migration, collagenization, epithelialization and angiogenesis scores compared to control group. Conclusions & Implications: Althaea officinalis can be used as an adjunct medication to attenuate wound healing in skin burns.

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Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v14i1.416 ◽

2015 ◽

Vol 14 (1) ◽

pp. 1

Author(s):

Muhammad Reza Setiawan ◽

Nurdiana Dewi ◽

Ika Kustiyah Oktaviyanti

Keyword(s):

Wound Healing ◽

Healing Process ◽

Negative Control ◽

Control Group ◽

Wound Healing Process ◽

Control Group Design ◽

Treatment Groups ◽

Accelerate Wound Healing ◽

Channa Striata ◽

Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract hassubstantial properties such as albumin, Zn,Cu, and Feto accelerate wound healing. The purpose of this study is to assessthe effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. Thisstudy was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatmentgroups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, eachtreatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of eachgroup was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant differencebetween negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increasedneocapillaries count in wound healing process.

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Investigating the healing effect of the hydroalcoholic extract of pomegranate seed (Punica granatum) on the full thickness wound in rabbit

Journal of Contemporary Medical Sciences ◽

10.22317/jcms.v6i2.715 ◽

2020 ◽

Vol 6 (2) ◽

Author(s):

Ali Asghar Hemmati ◽

Iran Rashidi ◽

Sedigheh Dahanzadeh ◽

Mahmoud Moeini

Keyword(s):

Wound Healing ◽

Healing Process ◽

Punica Granatum ◽

Negative Control ◽

Control Group ◽

Wound Healing Process ◽

Hydroxyproline Content ◽

Tissue Samples ◽

Treatment Groups ◽

Wound Recovery

Objective: This research designed to investigate the wound healing process with pomegranate hydroalcoholic seed extract (Punica granatum) in comparison with no-treatment, betamethasone, phenytoin and eucerin in rabbits. Methods: The positive group including groups that received phenytoin cream (1%) and topical eucerin, respectively, twice a day to complete wound healing. negative Control group did not obtain any treatment. Treatment groups were received cream of PSE (2, 5,7,10 w/w) in eucerin and 75% w/w as purified extract twice daily. In order to measure the percentage of wound healing, the zone of the wound was evaluated daily. Histological studies were done on the 7th and 15th days of treatment. Next, hydroxyproline content of wounds healed and tensile strength of wound tissue samples were measured. Results: The results demonstrated between PSE treatment groups and eucerin animals were statistically significant aadifferences (P<0.05) in most of the days reviewed. Treatment of Rabbits with 10% PSE had the best results (complete wound recovery in 12 days). Also, this treatment showed higher hydroxyproline content and higher tissue strength. Conclusion: This research reveals that the extract of 10% PSE administrated topically has the proper potential to induce wound recovery in the wound model of rabbits. In addition, 10% PSE accelerates the healing of the wound. Further study needs to clarify the results of this research.

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PENGARUH BALUTAN BASAH OLIVE OIL TERHADAP PROSES PENYEMBUHAN LUKA BAKAR DERAJAT II PADA TIKUS PUTIH (RATTUS NORVEGICUS)

Jurnal Keperawatan dan Kesehatan ◽

10.54630/jk2.v12i2.149 ◽

2021 ◽

Vol 12 (2) ◽

pp. 81-86

Author(s):

Didit Damayanti

Keyword(s):

Wound Healing ◽

Olive Oil ◽

Rattus Norvegicus ◽

Healing Process ◽

Control Design ◽

Wound Healing Process ◽

P Value ◽

White Rats ◽

Grade Ii ◽

True Experiment

Luka bakar yang sering terjadi dan banyak ditemukan adalah luka bakar derajat II. Tumbuhan alam yang berkhasiat obat telah banyak dikenal oleh masyarakat Indonesia sejak lama. Salah satu tanaman alam yang mengandung banyak khasiat adalah olive oil. Olive oil memiliki kandungan senyawa penting dalam penyembuhkan luka antara lain oleochantal, sterol, fenol, squalen, vitamin C, E dan K yang dapat berperan mempercepat proses penyembuhan luka. Kandungan vitamin A dan vitamin E telah diketahui sebagai vitamin yang dapat membantu dalam proses penyembuhan luka. Penelitian ini menggunakan olive oil yang di buat dalam bentuk balutan basah untuk perawatan luka bakar derajat II pada tikus putih. Penelitian ini menggunakan metode true experiment dengan desain posttest only control design. Penelitian ini dilaksanakan pada bulan Maret 2020. Sampel penelitian adalah 20 kelompok perlakuan (tikus putih yang diberikan balutan basah olive oil) dan 20 kelompok kontrol tikus putih. Dengan teknik random sampling. Data diolah dengan SPSS menggunakan uji Mann Whitney. Hasil penelitian di dapatka P-value 0,00 < α (0,05) yang berarti adanya pengaruh Perawatan Luka Bakar Derajat II Dengan Balutan Basah Olive Oil Pada Tikus Putih (Rattus Norvegicus). Diharapkan dengan pemanfaatan bahan alam untuk kepentingan sehari-hari dapat meminimalkan efek samping dari penggunaan bahan kimia yang sering digunakan oleh masyarakat saat ini. Kata Kunci: Luka Bakar Derajat II, Olive Oil, Tikus Putih (Rattus Norvegicus) Abstract Burns that often occur and are commonly found are second-degree burns. Natural medicinal plants have been widely known by the people of Indonesia for a long time. One of the natural plants that contain many benefits is olive oil. Olive oil has important compounds in wound healing including oleochantal, sterol, phenol, squalen, vitamins C, E and K which can play a role in accelerating the wound healing process. The content of vitamin A and vitamin E has been known as a vitamin that can help in the wound healing process. This study used olive oil which is made in the form of a wet dressing for the treatment of second-degree burns in white rats. This study used the true experiment method with the posttest only control design. The research was conducted in March 2020. The study sample was 20 treatment groups (white rats given wet olive oil dressing) and 20 control groups of white rats. With random sampling technique. Data were processed with SPSS using the Mann Whitney test. The results of the study obtained a P-value of 0.00 <α (0.05), which means that there is an influence of Grade II Burn Treatment with Olive Oil Wet Wrap on White Rat (Rattus Norvegicus). It is expected that the use of natural materials for daily use can minimize the side effects of the use of chemicals that are often used by the community today. Keywords: Grade II Burns, Olive Oil, White Rat (Rattus Norvegicus)

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COMPARISON OF THE EFFECTIVENESS OF GIVING BINAHONG LEAF (Anredera cordifolia (Ten) Steenis) AND PAPAYA LEAF (Carica papaya) ON SKIN WOUND HEALING IN WHITE RAT (Rattus novergicus)

Journal Widya Medika Junior ◽

10.33508/jwmj.v3i2.3181 ◽

2021 ◽

Vol 3 (2) ◽

pp. 78-85

Author(s):

Givenchy Winarjo ◽

◽

Fransiscus Arifin ◽

Dave Oenarta ◽

◽

...

Keyword(s):

Wound Healing ◽

Leaf Extract ◽

Healing Process ◽

Control Group ◽

Wound Healing Process ◽

P Value ◽

World Population ◽

White Rats ◽

Significant Difference ◽

The World

Introduction: The incidence of acute and chronic injuries increases every year. Most injuries in the world population, as many as 48%, are caused by trauma or surgery. the highest incidence of wounds in the world based on the etiology was from surgery, which was 110.30 million cases. One of the handlings of wounds on the skin can be done by giving herbal medicines, which can be easily accessed by the public, such as binahong leaves and papaya leaves. Purpose: To analyze the effectiveness of giving 40% binahong leaf extract cream and 40% papaya leaf extract cream on the wound distance in the white rat wound healing process. Method: this study is an experimental study with post-test only control group design approach. The sampling technique used in this study was a random sampling method where the number of samples was calculated using the Federer formula, with a total sample of 28 white rats. The distance of the wound was measured using a micrometer. Results: Twenty-eight wound distance on rats were measured as subjects of the study and then treated with binahong leaf extract cream and papaya leaf extract cream, then the wound distance of each treatment was measured using a micrometer. According to the results, 40% binahong leaf extract cream and 40% papaya leaf extract cream showed better effectiveness in reducing the wound distance of white rat incision than the negative control group (cream base) and positive control group (gentamicin ointment). According to the Mann Whitney test comparing the P1 group (40% binahong leaf extract cream) and P2 (40% papaya leaf extract cream), p-value >0.05, which is 0.543, which means that P1 has no significant mean difference with P2. Conclusion: There was no significant difference in effectiveness between the treatment of 40% binahong leaf extract cream and 40% papaya leaf extract cream.

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Ellagic Acid Activity in Healing Process of Incision Wound on Male Albino Rats (Rattus norvegicus)

KnE Life Sciences ◽

10.18502/kls.v3i6.1131 ◽

2017 ◽

Vol 3 (6) ◽

pp. 224

Author(s):

Hardany Primarizky ◽

Wiwik Misaco Yuniarti ◽

Bambang Sektiari Lukiswanto

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Ellagic Acid ◽

Healing Process ◽

Control Group ◽

Albino Rats ◽

Wound Healing Process ◽

Treatment Groups ◽

Significant Difference ◽

Incision Wound

This study was conducted to prove whether ellagic acid can be used as a regulator of incision wound healing process in male albino rats as experimental animals. A total of 24 male healthy rats based on physical examination, aged at 3 months old and weight at 150-180 grams used in this study. Rats were divided into three groups and each group has 8 replications, group of control (P0) has no incision wound and no medication, treatment group (P1) has incision wound and treated with Betadine® and (P2) has incision wound and treated with 2,5 % ellagic acid. The variable that observed are histopathology changes (collagen, PMN, angiogenesis and fibrosis) from the male albino rats. The samples collection was taken at day 15 and data were collected and analyzed by Analysis of Variance (ANOVA) test. The result of collagen and PMN cells score in the control group (P0) were 1.80 ± 0.16 and 1.6 ± 0.08 with mild angiogenesis and no fibrosis. The results in the treatment (P2) group, where the collagen and PMN scores were 1.60 ± 0.08 and 1.5 ± 0.8, accompanied by mild angiogenesis and fibrosis. Meanwhile, in the treatment (P1) group, the results of scoring of collagen and PMN were lower than the other groups, 0, 80 ± 0.08 and 0.6 ± 0.16 accompanied by moderate angiogenesis and fibrosis. There was a significant difference of collagen scoring, PMN cells number, fibrosis and angiogenesis among the treatment groups (p<0.05). P1 treatment group has significant different with treatment groups P0 and P2 (p<0.05), but there is no significant difference between treatment groups P0 and P2 (p>0.05). From the results, it can be concluded that the topical application of ellagic acid ointment was proved to provide healing process on incision wound. Topical administration of ellagic acid ointment for 14 days was able to improve the skin condition and promoted the wound healing.Keywords: Ellagic acid ; wound healing ; collagen ; angiogenesis ; fibrosis ; anti-inflammatory ; antioxidant ; antibacterial.

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Effect of haruan (Channa striata) extract on fibroblast cells count in wound healing

Journal of Dentomaxillofacial Science ◽

10.22208/jdmfs.1.2.2016.226-231 ◽

2016 ◽

Vol 1 (2) ◽

pp. 226

Author(s):

Ardi Siswanto ◽

Nurdiana Dewi ◽

Lisda Hayatie

Keyword(s):

Wound Healing ◽

Healing Process ◽

Negative Control ◽

Control Group ◽

Wound Healing Process ◽

Fibroblast Cells ◽

Control Group Design ◽

Treatment Groups ◽

Accelerate Wound Healing ◽

Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with aquadest for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p<0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

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Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

Journal of Dentomaxillofacial Science ◽

10.22208/jdmfs.v14i1.1 ◽

2015 ◽

Vol 14 (1) ◽

pp. 1

Author(s):

Muhammad Reza Setiawan ◽

Nurdiana Dewi ◽

Ika Kustiyah Oktaviyanti

Keyword(s):

Wound Healing ◽

Healing Process ◽

Negative Control ◽

Control Group ◽

Wound Healing Process ◽

Treatment Groups ◽

Accelerate Wound Healing ◽

Significant Difference ◽

Channa Striata ◽

Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties suchas albumin, Zn,Cu, andFeto accelerate wound healing. The purposeof this study is to assess the effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. This study was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of each group was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant difference between negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increased neocapillaries count in wound healing process.Haruan adalah ikankhas Kalimantan yangberpotensi mempercepat penyembuhanluka. Ekstrak ikan haruan mengandung zat penting seperti albumin, Zn , Cu, dan Fe untuk mempercepat penyembuhan luka. Penelitian ini dimaksudkan untuk mengetahui pengaruh ekstrak ikan haruan pada proses penyembuhan luka pada mukosa bukal tikus wistar hari ke-1, 3, 5, dan 7 secara histopatologi. Penelitian eksperimental murni dengan pretest-posttest with control group ini, meliputi 4 kelompok,yaitu kelompok hewan yang diberi ekstrak haruan selama7 hari dengan konsentrasi berturut-turut 25%, 50%, 100%, dan kelompok kontrol negatif diberi akuades. Hasilnya menunjukkan bahwa jumlah neokapiler mencapai puncak pada hari ke-5. Rerata jumlah neokapiler pada tiap kelompok berturut adalah 12; 16,5; 18,5; dan 6. Uji Anova 2-jalur dan uji post hoc LSDmenunjukkan adanya perbedaan bermakna antara kontrol negatif dan ekstrak ikan haruan. Berdasarkan hasil penelitian, disimpulkan bahwa pemberian ekstrak ikan haruan secara bermakna meningkatkan jumlah neokapiler pada proses penyembuhan luka.

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Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: in vivo studies in wistar rats

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v49.i3.p125-132 ◽

2016 ◽

Vol 49 (3) ◽

pp. 125

Author(s):

Gunawan Oentaryo ◽

Istiati Istiati ◽

Pratiwi Soesilawati

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Healing Process ◽

Tissue Growth ◽

Control Group ◽

Wound Healing Process ◽

Lower Lip ◽

Treatment Groups ◽

Collagen Secretion ◽

Channa Striata

Background: Wound healing is a biological process associated with tissue growth and regeneration. Wound healing process, is important to repair damaged tissue. Wound healing process consists of coagulation and hemostasis, inflammation, proliferation, as well as remodeling phases. The process can be accelerated by taking synthetic or non synthetic drugs. One of them is Channa striata extract. The extract contains albumin, copper, and zinc, which can be assumed to increase inflammatory cell infiltration, fibroblast proliferation, and collagen secretion. Purpose: This study aimed to reveal the effects of Channa striata extracts on fibroblast number and FGF-2 expression in mucosal wound healing process of the Wistar rats’ lower lip. Method: This research was a true laboratory experimental research with randomized post test only control group design. Samples of experiment were devided to experiment and control group that consist five samples each. Experimental group was treted with Channa striata extract and ethanol at concentration of 25%, 50%, and 100%. The fibroblast number and FGF-2 expresion were examined. Result: The number of fibroblasts in the treatment groups receiving Channa striata extract at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest number of fibroblasts was found on day 3 at the concentration of 100% (p<0.05). Similarly, FGF-2 expression in the treatment groups receiving Channa striata at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest expression of FGF-2 was found on day 3 at the concentration of 50% (p<0.05). Conclusion: Channa striata extract increased fibroblast number and FGF-2 expression in mucosa wound healing process.

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