Antibacterial activity of Stichopus hermanii and Stichopus variegatus methanol extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

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Antibacterial Activity of Nanoparticles Produced by Ethanol Extract of Boesenbergia rotunda Rhizome Loaded with Chitosan and Alginic Acid Against Streptococcus mutans by In Vitro

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v7i03.9554 ◽

2017 ◽

Vol 7 (03) ◽

Author(s):

Sri Atun ◽

Sri Handayani ◽

Melia Aliffiana ◽

Hajar Nur Afifah ◽

Anna Rakhmawati

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Agent ◽

Methanol Extract ◽

Alginic Acid ◽

Ethanol Extract ◽

Diffusion Method ◽

E Coli ◽

Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

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Identification of Secondary Metabolites and Antibacterial Activity of Non Polar Fraction from Heterotrigona itama Propolis

Journal of Fundamental and Applied Pharmaceutical Science ◽

10.18196/jfaps.v2i1.12406 ◽

2021 ◽

Vol 2 (1) ◽

pp. 23-33

Author(s):

Abdul Aziz ◽

Veggy Nadya Yuliawan ◽

Paula Mariana Kustiawan

Keyword(s):

Antibacterial Activity ◽

Methanol Extract ◽

Phenolic Content ◽

Polar Fraction ◽

Hexane Fraction ◽

Diffusion Method ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Phytochemical Content ◽

Color Changes

Propolis is one of the natural products produced by kelulut bees and is still not widely used. The type of stingless bee that is the prima donna in the community is Heterotrigona itama. This study aims to determine the phytochemical content of the n-hexane fraction of Heterotrigona itama bee propolis collected from Kutai Kartanegara, East Kalimantan. The n-hexane fraction was obtained from the methanol extract of H. itama propolis by the liquid-liquid partition method. After obtaining the n-hexane fraction, the research continued with a qualitative phytochemical test to identify the compound and determine total phenolic. Antibacterial activity was determined by the agar well diffusion method with a serial concentration in Escherichia coli bacteria. Qualitative phytochemical analysis in the form of color changes showed that the n-hexane fraction of H. itama propolis contained flavonoids, alkaloids, saponins, and tannins. Based on the results, the total phenolic content of the n-hexane fraction sample was 490 mgGAE/100 g. It caused the n-hexane fraction to have lower phenolic content than the methanol extract (792 mg GAE100 g). Furthermore, this result indicated that the non-polar fraction was not substantial enough to extracted phenolic compounds. It correlated to the antibacterial activity of the n-hexane fraction, which was very weak (2 mm ± 1.5) at 200µg/mL concentration.

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EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

10.36106/paripex/2805137 ◽

2021 ◽

pp. 77-79

Author(s):

Janardhan Namdeo Nehul

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Methanol Extract ◽

Axenic Culture ◽

Soil Samples ◽

Diffusion Method ◽

Disc Diffusion ◽

Zone Of Inhibition ◽

Disc Diffusion Method ◽

Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

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ANALYSIS OF SECONDARY METABOLITES, ANTIBACTERIAL ACTIVITY AND COMPOUND COMPOSITION IN THE SEA CUCUMBER Bohadschia sp. EXTRACT

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v8i2.15831 ◽

2017 ◽

Vol 8 (2) ◽

pp. 645-653 ◽

Cited By ~ 1

Author(s):

Abdullah Rasyid

Keyword(s):

Antibacterial Activity ◽

Bacillus Subtilis ◽

Secondary Metabolites ◽

Sea Cucumber ◽

Similarity Index ◽

Diffusion Method ◽

Composition Analysis ◽

Activity Test ◽

Agar Diffusion Method ◽

Color Reactions

Bohadschia sp. is one of the sea cucumber species that has potential to be developed as a source of antibacterial from the sea. Samples of sea cucumber Bohadschia sp. used in this study collected from the Ratai bay waters, Lampung. This study aims to determine the type of secondary metabolites, antibacterial activity and compound composition analysis containing in the sea cucumber extract. Identification of secondary metabolites by observation of color reactions, precipitation and foam. The method used to antibacterial activity test was the agar diffusion method, while identification of the composition of compounds performed with Gas Chromatogaphy-Mass Spectroscopy (GC-MS) method.Top of FormThe results showed that the type of secondary metabolites contained in the extract of sea cucumber Bohadschia sp. were steroids and saponins. The extract of sea cucumber Bohadschia sp. showed antibacterial activity against Bacillus subtilis and Vibrio eltor. Results of GC-MS were 12 compounds and have a similarity index same or more than 90%. All compounds consist of organosilicon cyclic, fatty acid, steroid, cyclo alkene and alkena. The compound with biggest abundance was cholest-5-en-3-yl nonanoate (4.89%) and retention time was 37.370 minutes.

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A novel antibacterial agent based on AgNPs and Fe3O4 loaded chitin microspheres with peroxidase-like activity for synergistic antibacterial activity and wound-healing

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2018.10.002 ◽

2018 ◽

Vol 552 (1-2) ◽

pp. 277-287 ◽

Cited By ~ 19

Author(s):

Ningxiang Yu ◽

Taimei Cai ◽

Yong Sun ◽

Chengjia Jiang ◽

Hua Xiong ◽

...

Keyword(s):

Wound Healing ◽

Antibacterial Activity ◽

Antibacterial Agent ◽

Agent Based ◽

Synergistic Antibacterial Activity

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Uji Aktivitas Antibakteri Ekstrak Metanol dan Fraksi Daun Kenikir (Cosmos caudatus Kunth) terhadap Salmonella typhi

Pustaka Kesehatan ◽

10.19184/pk.v8i3.13008 ◽

2020 ◽

Vol 8 (3) ◽

pp. 177

Author(s):

Achmad Syarifudin Noor ◽

Bawon Triatmoko ◽

Nuri Nuri

Keyword(s):

Antibacterial Activity ◽

Typhoid Fever ◽

Ethyl Acetate ◽

Methanol Extract ◽

Inhibition Zone ◽

Salmonella Typhi ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Water Fraction ◽

Zone Diameter

The leaves of kenikir are known to have many benefits, one of which can be used as medicine. Kenikir leaves are reported to have antibacterial activity against the bacteria Salmonella typhi (S.typhi). S. typhi is a bacterium that causes typhoid fever. Typhoid fever is still an endemic disease in Asian countries, especially Indonesia. Typhoid fever became the third largest disease in Indonesia in 2010. The study related to the antibacterial activity of kenikir leaf fraction to S. typhi has never been reported. Therefore, it is necessary to conduct research related to the antibacterial activity of extracts and leaf fractions of kenikir to compare their potential against S. typhi. The study was conducted using the disk diffusion method using 5 series of concentrations namely 5%, 10%, 15%, 20%, and 30%. Tests were carried out on methanol extract, hexane, ethyl acetate, and methanol-water fraction. The results showed that highest inhibition zone diameter is 7,75 mm which is reached by methanol extract. The order of the highest inhibiting zone diameter are extract, hexane, ethyl acetate, and methanol-water fraction.

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UJI AKTIVITAS ANTI BAKTERI ASAP CAIR YANG BERASAL DARI BATANG KAYU MANIS DAN KULIT KACANG TANAH

Jurnal Riset Kimia ◽

10.25077/jrk.v2i2.161 ◽

2015 ◽

Vol 2 (2) ◽

pp. 190

Author(s):

Yefrida ◽

Farrah Aprilina ◽

Indri Ticel Leone ◽

Refilda

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Agent ◽

Diffusion Method ◽

Liquid Smoke ◽

Growth And Reproduction

ABSTRAK Antibacterial agent is a compound that prevent the growth and reproduction of bacteria. Antibacterial activity of liquid smoke is caused by acid and fenolic compound. In this research, we determined antibacterial activity of liquid smoke from stem sweet wood and nut shell by using diffusion method. Both of liquid smoke have antibacterial activity to Staphylococcus aureus Eschericia coli population. Their activity almost the same to chloramphenicol, but less than formalin. Keywords : antibacterial activity, liquid smoke, diffusion method

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ANTIBACTERIAL ACTIVITY FOR MULTI DRUG RESISTANCE (MDR) BACTERIA BYSEA CUCUMBER Stichopus vastus EXTRACT FROM KARIMUNJAWA ISLANDS – INDONESIA

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v9i2.19302 ◽

2018 ◽

Vol 9 (2) ◽

pp. 695-707 ◽

Cited By ~ 1

Author(s):

Delianis Pringgenies ◽

Ali Ridlo ◽

Nerva Sembiring

Keyword(s):

Drug Resistance ◽

Antibacterial Activity ◽

Sea Cucumber ◽

Inhibition Zone ◽

Extraction Process ◽

Sensitivity Test ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Multi Drug Resistance ◽

Bacterial Sensitivity

The study aims to explore the antibacterial activity of Stichopus vastus against pathogenic MDR bacteria. Analysis of samples of sea cucumbers included extraction, fractionation, and analysis of bacterial sensitivity test Gas Chromatography-Mass Spectrometry (GC-MS), the extraction process is carried out by solid-liquid extraction method. Fractionation was done with Open-Column Chromatography (OCC). Sensitivity test of bacteria was done using an agar diffusion method according to the Kirby-Bauer (Ref). The study revealed that from 5 species MDR bacteria, which are Coagulant negative stapylococi (CNS), E. coli, Enterobacter 5, Klebsiella sp. dan Pseudomonas sp. There are two MDR bacteria had the most sensitive responses by the extract of sea cucumber, which were Enterobacter-5 and Klebsiella sp. The two bacteria were tested against five bioactive fractions obtained from OCC. Fraction criteria-2 had the highest antibacterial activity against Enterobacter-5 and Klebsiella sp, with serial concentration of 20 µg ∙ disc–1, 40 µg. disc–1 and 80 µg. disc–1. Largest inhibition zone were obtained from 80 µg. disc–1 againts the two bacteria were (14.73 ± 0.48) mm and (11.22 ± 0.85) mm respectively. GC-MS Analysis revealed that fraction criteria-2 had (or consisted of) cyclohexhane, ethanol, butanoic and pentanoic acids. Keywords: antibacterial activity, multi drug resistance (MDR), sea cucumber, Stichopus vastus

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Cinnamon Oil as an Antibacterial Agent on Natural Paper from Banana Peel (Musa paradisiaca Linn.)

JKPK (Jurnal Kimia dan Pendidikan Kimia) ◽

10.20961/jkpk.v4i1.26120 ◽

2019 ◽

Vol 4 (1) ◽

pp. 42

Author(s):

Widiastuti Agustina Eko Setyowati ◽

Elfi Susanti VH ◽

Iis Alviah ◽

Melati Kusuma Wardani

Keyword(s):

Antibacterial Activity ◽

Water Content ◽

Antibacterial Agent ◽

Antibacterial Activities ◽

Diffusion Method ◽

Banana Peel ◽

Cinnamon Oil ◽

E Coli ◽

Musa Paradisiaca ◽

Oil Additives

The research about the use of cinnamon oil as an antibacterial agent on natural paper from Banana (musa paradisiaca Linn.) Peel has been conducted. The preparation of the paper using alkalization method, with the use of NaOH 4% at the temperature of 100oC for 1.5 hours. The paper is made in two variations, ie with additive cinnamon oil and without additive. The natural paper produced are tested for pH based on SNI ISO 6588-1.2010 and water content by dry-oven method based on SNI ISO 287:2010. The antibacterial activities were tested against Staphylococcus aureus and Escherichia coli using Kirby-Bauer disc diffusion method. The paper produced meet the standard of ISO 6519:2016 about Basic Paper for Laminated Plastic Wrapping Paper within the parameter of pH and water content. The results showed that the paper with additive cinnamon oil have antibacterial activity againts S. aureus and E. coli with inhibition diameter zone 15 mm and 13 mm respectively, whereas paper without cinnamon oil additives has no antibacterial activity against either S. aureus or E. coli. These result indicate that cinnamon oil can be used as an antibacterial agent on the natural paper from Banana peel (musa paradisiaca Linn.).

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