scholarly journals Antibacterial effects of methanolic extracts of Reum ribes L. and Hyssopus officinalis on some standard pathogenic bacteria

2019 ◽  
Vol 7 (3) ◽  
pp. 34-44
Author(s):  
javad sayyahi ◽  
Hayedeh Mobaiyen ◽  
behboud Jafari ◽  
Abolfazl Jafari-Sales ◽  
◽  
...  
2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Seyede Asma Hosseini Chaleshtori ◽  
Mehrdad Ataie Kachoie ◽  
Seyed Mojtaba Hashemi Jazi

High occurrence of antibiotic resistance in pathogenic bacteria is caused by the use of natural medicinal plants to eliminate risk of infectious diseases. Glycine max is on the most popular and nutritious foods with high antimicrobial effects. The present research was done to study the antibacterial effects of the methanolic extract of G. max against Gram-negative and positive bacteria. G. max seeds of M7 and M9 varieties were purchased and their methanolic extracts were collected. Diameter of the zone of growth inhibition of extracts were measured for pathogenic bacteria using the CLSI guidelines. Antimicrobial effects of G. max extracts were also compared with several antibiotics. Diameter of the inhibition zone of tested bacteria against different treatments of G. max had a ranges of 5.93±0.18 to 22.61±1.80 mm. M7 variety had a higher antimicrobial effects on tested bacteria (P0.05). The highest diameter of the zone of growth inhibition was seen for L. monocytogenes (22.61±1.80 mm) and S. aureus (19.33±1.56 mm). Antimicrobial effects of the methanolic extract of the G. max was also dose-dependent (P0.05). Diameter of the inhibition zone of tested bacteria against different antibiotic agents had a ranges of 5.94±0.38 to 24.95±2.11 mm. Ciprofloxacin, penicillin G tetracycline, gentamicin and azithromycin antibiotic agents exhibited the highest diameter of zone of the growth inhibition for K. pneumoniae (15.71±1.46 mm), L. monocytogenes (23.94±2.03 mm), L. monocytogenes (20.55±1.95 mm), S. aureus (14.27±1.36 mm) and L. monocytogenes (24.95±2.11 mm), respectively. Formulation of antibiotic agent using the 100 mg/mL concentration of the M7 variety of G. max has been recommended for treatment of the cases of infectious diseases


Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 871
Author(s):  
Wen-Jie Ng ◽  
Nam-Weng Sit ◽  
Peter Aun-Chuan Ooi ◽  
Kah-Yaw Ee ◽  
Tuck-Meng Lim

Scientific studies about the antibacterial effects of honeydew honey produced by the stingless bee are very limited. In this study, the antibacterial activities of 46 blossom and honeydew honeys produced by both honey bees and stingless bees were evaluated and compared. All bacterial isolates showed varying degrees of susceptibility to blossom and honeydew honeys produced by the honey bee (Apis cerana) and stingless bee (Heterotrigona itama and Geniotrigona thoracica) in agar-well diffusion. All stingless bee honeys managed to inhibit all the isolates but only four out of 23 honey bee honeys achieved that. In comparison with Staphylococcus aureus, Escherichia coli was found to be more susceptible to the antibacterial effects of honey. Bactericidal effects of stingless bee honeys on E. coli were determined with the measurement of endotoxins released due to cell lysis. Based on the outcomes, the greatest antibacterial effects were observed in honeydew honey produced by H. itama. Scanning electron microscopic images revealed the morphological alteration and destruction of E. coli due to the action of this honey. The combination of this honey with antibiotics showed synergistic inhibitory effects on E. coli clinical isolates. This study revealed that honeydew honey produced by H. itama stingless bee has promising antibacterial activity against pathogenic bacteria, including antibiotic resistant strains.


2004 ◽  
Vol 59 (9-10) ◽  
pp. 657-662 ◽  
Author(s):  
Juliana B. Pretto ◽  
Valdir Cechinel-Filho ◽  
Vânia F. Noldin ◽  
Mara R. K. Sartori ◽  
Daniela E. B Isaias ◽  
...  

Abstract Calophyllum brasiliense (Clusiaceae/Guttiferae) is a native Brazilian medicinal plant traditionally used against several diseases, including infectious pathologies. Crude methanolic extracts (CME) and two fractions, denoted non-polar (soluble in chloroform) and polar (nonsoluble in chloroform), were prepared from different parts of the plant (roots, stems, leaves, flowers and fruits) and studied. The following compounds were isolated and tested against pathogenic bacteria and yeasts by determination of the minimal inhibitory concentration (MIC): brasiliensic acid (1), gallic acid (2), epicatechin (3), protocatechuic acid (4), friedelin (5) and 1,5-dihydroxyxanthone (6). The results indicated that all the parts of the plant exhibited antimicrobial activity against Gram-positive bacteria, which are selectively inhibited by components of C. brasiliense. No activity was observed against Gram-negative bacteria and yeasts tested. Regarding the isolated compounds, substance 4 showed antimicrobial activity against all the tested microorganisms, whereas compound 6 exhibited antimicrobial activity only against Gram-positive bacteria. The results from the current study confirm and justify the popular use of this plant to treat infectious processes.


2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Neda Jam ◽  
Reza Hajimohammadi ◽  
Parvin Gharbani ◽  
Ali Mehrizad

Today, the tendency to use of natural preservatives to increase food security has expanded. In the present study, antibacterial effects of Areca Nut fruit extracts were evaluated against Staphylococcus aureus, Escherichia coli, Salmonella enterica, and Enterobacter aerogenes bacteria using agar disc diffusion technique. Methanol, ethanol, and water were used as solvents for extraction by maceration method, and extracts were analyzed by GC-MS. The antibacterial activity was evaluated using microtiter broth dilution method to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results revealed that all ATCC strains were significantly inhibited by ethanolic and methanolic extracts. Escherichia coli produced a significantly larger zone of inhibition for Gentamicin ( 35 ± 0.65   mm ) and Penicillin ( 25   mm ± 0.56 ), while Enterobacter aerogenes produced smaller zone of inhibition for Gentamicin (20 ± 0.87 mm) and Penicillin ( 15 ± 0.87   mm ). Also, methanolic extract had considerable antibacterial activity with MIC value of 1.56 mg/mL against Escherichia coli. All of extracts were used to evaluate antibacterial effects in prepared cake, and as a result, all pathogenies were the most sensitive by methanolic extract in 100 mg/L of concentration except Escherichia coli that were more sensitive by ethanolic extract. In conclusion, the Areca Nut fruit extracts may be used as a natural preservative in food industries. Future studies should focus on the effect of Areca Nut fruit extracts in bakery and drinking industries.


2019 ◽  
Author(s):  
Maria Cheraghi ◽  
Fatemeh Babadi

Abstract Recently, natural products have been evaluated as a source of antimicrobial agent with efficacies against a variety of microorganisms. The antibacterial activities of the oak fruit,inner husk of oak fruit (Jaft), and Jaftex mouthwash have been studiedin several studies. Thisstudy aimedto review the studies ofthe effects of antibacterial properties of the oak fruit, oak fruit hull (Jaft), and Jaftex mouthwash. Materials and Methods In this review study, relevant articles related to the antimicrobial activity of the oak fruit, inner husk of oak fruit (Jaft), and 'Jaftex mouthwash' were searched from the current digital literature using electronic databases namely, SID, ScienceDirect, PubMed, Google Scholar, Magiran, Web of Science employing the same keywords from 1990 to 2019. Results Oakfruit, inner husk of oak fruit (Jaft), and Jaftex mouthwash have antimicrobial properties against many microorganisms. Discussion and Conclusion Antibacterial properties of oak fruit, inner husk ofoak fruit (Jaft) can be used to reverse the antibiotic sensitivity against pathogenic bacteria. Jaftex is recommended as an antibacterial and anti-plaque mouthwash.


Author(s):  
Lela Lailatul Khumaisah ◽  
Lela Mukmilah Yuningsih ◽  
Asep Kadarohman

The chemical content in essential oils have activities on microbes, biopesticides, pathogenic vector insects in humans and animals, including Mentha arvensis and Mentha piperita (mint plants) which have antifertility and antibacterial effects, Vetiveria zizanoides (vetiver root) as insects repellent, Cymbopogon nardus (lemongrass) has an activity against plant pathogenic bacteria and antifungals, and Rosmarinus officinalis (rosemary) has locomotor activity of mice and antibacterial. Essential oils are effective against target organisms, compatible, safe and non-toxic, so they have enormous potential to be developed as biopesticides in controlling rice pests. On the other hand, pests and diseases are problems that always disturb rice farmers, including in Sukaresmi and Kebon Pedes villages, Sukabumi. Therefore, the use of essential oil plants has been carried out to control rice mouse pests. This has been started from the preparation of seeds to process of planting the four types of essential oils (mint, lemongrass, and rosemary) in the fields as intercrops and the procurement of essential oil distillation sets. Based on observations, the growth and development of essential oil plants as intercropping plants on rice runs normally and its impact on rice pests, especially in mice, is quite significant with not found rats around the planting area.   Keywords: Mint, lemongrass, rosemary, essential oil, biopesticide


2021 ◽  
pp. 147-152
Author(s):  
Laila Ben Ramadan ◽  
Abdurzag Zwawi ◽  
Mohamed Salem ◽  
Abdurzag Auzi ◽  
Ahmed El Marghani

Bacterial resistance develops due to overuse of antibiotics in addition to the adverse effects of these chemicals. This urges the scientists to exchange these antibiotics with alternatives from natural products. The study aimed to evaluate the antimicrobial efficiency of Arum cyrenicum Hruby against a number of medically important pathogenic bacteria. A. cyreniacum corms extracted with petroleum ether, chloroform and methanol, and tested for antimicrobial activity against Escherichia coli BTC3, Salmonella typhi BTC10, Pseudomonas aeruginosa BTC4, and Staphylococcus aureus BTC15. A considerable bactericidal efficiency of petroleum ether extract of A. cyrenaicum corms were specifically against gram-positive bacteria, Staphylococcus aureus BTC15 at (100 mg/ml) petroleum ether and bacteriostatic at (50 mg/ml) of petroleum Ether extract. Whereas, the methanolic extract of A. cyrenaicum corms had bactricidal effect against gram-negative bacteria, Pseudomonas auregenosa BTC4 at (100mg/ml) of methanolic extract. Herby our results indicate that petroleum ether and methanolic extracts of A. cyrenaicum possess significant antibacterial properties. Keywords: Arum cyrenicum, Efficiency, Antibacterial


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