scholarly journals Antibacterial effects of the methanolic extract of Glycine Max (Soybean)

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Seyede Asma Hosseini Chaleshtori ◽  
Mehrdad Ataie Kachoie ◽  
Seyed Mojtaba Hashemi Jazi
Keyword(s):  
Glycine Max ◽  
Infectious Diseases ◽  
Growth Inhibition ◽  
Pathogenic Bacteria ◽  
Methanolic Extract ◽  
Inhibition Zone ◽  
Penicillin G ◽  
Antibacterial Effects ◽  
Antimicrobial Effects ◽  
Antibiotic Agents

High occurrence of antibiotic resistance in pathogenic bacteria is caused by the use of natural medicinal plants to eliminate risk of infectious diseases. Glycine max is on the most popular and nutritious foods with high antimicrobial effects. The present research was done to study the antibacterial effects of the methanolic extract of G. max against Gram-negative and positive bacteria. G. max seeds of M7 and M9 varieties were purchased and their methanolic extracts were collected. Diameter of the zone of growth inhibition of extracts were measured for pathogenic bacteria using the CLSI guidelines. Antimicrobial effects of G. max extracts were also compared with several antibiotics. Diameter of the inhibition zone of tested bacteria against different treatments of G. max had a ranges of 5.93±0.18 to 22.61±1.80 mm. M7 variety had a higher antimicrobial effects on tested bacteria (P0.05). The highest diameter of the zone of growth inhibition was seen for L. monocytogenes (22.61±1.80 mm) and S. aureus (19.33±1.56 mm). Antimicrobial effects of the methanolic extract of the G. max was also dose-dependent (P0.05). Diameter of the inhibition zone of tested bacteria against different antibiotic agents had a ranges of 5.94±0.38 to 24.95±2.11 mm. Ciprofloxacin, penicillin G tetracycline, gentamicin and azithromycin antibiotic agents exhibited the highest diameter of zone of the growth inhibition for K. pneumoniae (15.71±1.46 mm), L. monocytogenes (23.94±2.03 mm), L. monocytogenes (20.55±1.95 mm), S. aureus (14.27±1.36 mm) and L. monocytogenes (24.95±2.11 mm), respectively. Formulation of antibiotic agent using the 100 mg/mL concentration of the M7 variety of G. max has been recommended for treatment of the cases of infectious diseases

In-vitro Antibacterial Effects of Aqueous, Ethanolic, Methanolic, Acetone and Hydro Ethanolic Extracts of Achillea Millefolium on Standard Klebsiella, S. pyogenes, and Oral Bacterias Strains

2020 ◽  
Author(s):  
Negar Fallah ◽  
Sanaz Namazi ◽  
Negar Balmeh ◽  
Samira Mahmoudi ◽  
Fereshteh Mirzaei Poor
Keyword(s):  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Methanolic Extract ◽  
Growth Zone ◽  
Achillea Millefolium ◽  
Antibacterial Effects ◽  
Millipore Filter ◽  
Antimicrobial Effects ◽  
Aerial Parts

Introduction: So far, a lot of attempts have been carried out to find antimicrobial compounds. In this study, it was also tried to investigate the antibacterial effects of Achillea millefolium on standard Klebsiella, S. pyogenes, and oral bacterias strain. Material and Methods: The aerial parts of Achillea millefolium were used and the aqueous, ethanolic, methanolic, acetone and hydroethanolic extracts were prepared. After the preparation of standard strains of Klebsiella, S. pyogenes, and oral bacterias and sterilization of extracts by the Millipore filter, the antibacterial effects of these extracts on the mentioned microorganisms were assessed by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and well diffusion at the concentration 50 mg/ml. The test was repeated three times for each bacterium. Results: It was exposed that aqueous extract of Achillea millefolium had the most distinguished antimicrobial effects against all studied strains and methanolic extract had antimicrobial effects only on S. pyogenes. MIC and MBC of effective extracts were the basic concentration (50mg/ml), and non-growth zone was not observed in other serial dilution in case of all bacteria. Conclusion: The Achillea millefolium can be admitted as an antibacterial medicinal herb. Thus, it can be concluded that after evaluating their effects in vitro, Achillea millefolium can be utilized as an alternative to the routine chemical drugs.

scholarly journals Antibacterial Effects of Some Antiseptics and Disinfectants

1970 ◽  
pp. 19-21 ◽  
Author(s):  
AK Saha ◽  
MF Haque ◽  
S Karmaker ◽  
MK Mohanta
Keyword(s):  
Hydrogen Peroxide ◽  
Key Words ◽  
Pathogenic Bacteria ◽  
Antibacterial Effects ◽  
Antimicrobial Effects ◽  
Highly Effective

Antimicrobial effects of six antiseptics and disinfectants were studied against five pathogenic bacteria. Different pathogens responded differently to different antiseptics and disinfectants. Antibacterial effects of the antiseptics and disinfectants were also concentration dependent. Formalin and hydrogen peroxide were highly effective against all the pathogens used while phenyl and iodine were least effective. Dettol and Savlon showed moderate antibacterial effects. Key words: Antiseptics; disinfectants; pathogen; sensitivity. DOI: 10.3329/jles.v3i0.7440 J. Life Earth Sci., Vols. 3-4: 19-21, 2009

scholarly journals Molecular identification of Bifidobacterium sp. from local yoghurt and evaluation of growth inhibition activity against pathogenic bacteria

2021 ◽  
Vol 56 (3) ◽  
pp. 147-154
Author(s):  
AP Talukder ◽  
MN Haque ◽  
ML Mahmud ◽  
MAE Ekram
Keyword(s):  
Growth Inhibition ◽  
Molecular Identification ◽  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Antagonistic Activity ◽  
E Coli ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Acinetobacter Sp

Yoghurt is a potential source of probiotic bacteria including Bifidobacterium sp. In this context, sour yoghurt sample was collected from local market in Rajshahi for molecular identification and characterization of Bifidobacterium sp. with promising antagonistic activity against pathogenic bacteria. Isolation was done on Luria broth agar media for molecular identification and revealed that isolated bacterium showed 90% similarity with Bifidobacterium sp. Antibiotic sensitivity test result revealed that isolated Bifidobacterium sp. was sensitive to erythromycin, kanamycin, gentamycin, tetracycline, ciprofloxacin, doxycycline out of eleven commercially used antibiotics. Moreover, antagonistic activity of Bifidobacterium sp. was evaluated in our present study against four pathogenic bacteriathrough disc diffusion method. Bifidobacterium sp. had relatively strong antagonistic effect (inhibition zone ≥15 mm) against Salmonella sp. with 16mm and 19mm zones of inhibition at doses of 150 and 200 μg/disc, respectively. Similarly, the isolate showed strong growth inhibitory activity against Acinetobacter sp. and E. coli with inhibition zone of 17 mm and 16 mm at dose of 200 μg/disc while moderate growth inhibitory activity was observed against Aeromonas sp. at applied four doses. Furthermore, present investigation showed that the isolated Bifidobacterium sp. had the utmost effect against Salmonella sp. and exhibited growth inhibition of understudy pathogens in such pattern Salmonella sp.>Acinetobacter sp.> E. coli> Aeromonas sp. Bangladesh J. Sci. Ind. Res.56(3), 147-154, 2021

scholarly journals PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI EKSTRAK ETANOL DAUN BIDURI SECARA IN VITRO

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Nyoman Mastra
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone ◽  
The Difference

ABSTRACTBackground Staphylococcus aureus is a pathogenic bacteria that can cause infection. Biduri leaf has antibacterial compound such as tannin, flavonoid, saponin, and polyphenol.Objective This study aimed to know the difference of growth inhibition zone of Staphylococcus aureus at the various concentration of ethanol extract of biduri leaf and to determine an effective concentration.Methods This study was the true experiment with posttest only control design, used Kirby-Bauer disk diffusion method with five concentrations (20%, 40%, 60%, 80%,100%), positive control (chloramphenicol 30 µg) and negative control (ethanol 96%).Result The result of this study showed the average of inhibition zone diameter of each concentration consecutively 26,2 mm, 28,3 mm, 29,7 mm, 31 mm and 31,5 mm. One Way Anova statistic analysis showed that the value of p is 0.000 so there is the difference of growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of a biduri leaf.Conclusion The conclusion of this study is there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of biduri leaf and an effective concentration that can inhibit of Staphylococcus aureus is concentration 20% with mean inhibition zone is 26,2 mm. Keywords: ethanol extract of biduri leaf; Staphylococcus aureus; inhibition zone

scholarly journals Bioactivity and Phytochemical Studies of Seed Extracts of Anethum graveolens Linn.

2021 ◽  
Vol 11 (2) ◽  
pp. 3560-3572
Keyword(s):  
Infectious Diseases ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Enterobacter Aerogenes ◽  
Inhibition Zone ◽  
Nutritional Supplement ◽  
Salmonella Typhi ◽  
Anethum Graveolens ◽  
Synthetic Drugs

Infectious diseases cause enormous morbidity and mortality of the population worldwide every year. The increase in the rate of infectious diseases and bacteria attaining resistance to antibiotics and side effects of some synthetic antibiotics has led to an increased interest in medicinal plants as a better and natural alternative to synthetic drugs. Antibacterial activity of solvent extracts of Anethum graveolens L. was evaluated by agar cup diffusion and disc diffusion methods against some common pathogenic bacteria, namely, Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis, Bacillus cereus, Salmonella typhi, and Enterobacter aerogenes. The antioxidant capacity of different extracts of Anethum graveolens was estimated by diphenyl picryl hydrazyl (DPPH), nitric oxide (NO), and hydrogen peroxide. Solvent extracts of A. graveolens recorded a good 18-19 mm inhibition zone activity with methanol extract. The MIC of A. graveolens methanol extract was recorded in the range 125-1667 µg/ ml for test pathogens. The IC50 values showed that methanol extract was nearly potent to butylated hydroxyanisole (BHA) followed by ethyl acetate extracts in all the methods. Methanol extracts of A. graveolens possess a broad spectrum of activity against several human pathogenic and potent antioxidant properties. A natural substance obtained from plants that is a part of a daily diet, a nutritional supplement with antimicrobial and antioxidant properties, constitutes a new source of herbal drugs.

scholarly journals Isolasi Bakteri Endofit dari Tanaman Sirih Hijau (Piper betle L.) dan Potensinya sebagai Penghasil Senyawa Antibakteri

2017 ◽  
Vol 1 (1) ◽  
pp. 51-57
Author(s):  
Ukhradiya Magharaniq Safira ◽  
Fachriyan Hasmi Pasaribu ◽  
Maria Bintang
Keyword(s):  
Escherichia Coli ◽  
Endophytic Bacteria ◽  
Host Plants ◽  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Chemical Compounds ◽  
Inhibition Zone ◽  
Piper Betle ◽  
Antibacterial Effects ◽  
Potential Activity

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing disruption or damage to the host. Some studies suggest that certain endophytic bacteria can produce chemical compounds that have an effect on health, especially endophytic bacteria isolated from medicinal plants. Green betel (Piper betle L.) is a medicinal plant that has been used for years and has many benefits. The purposes of this study are to isolating and screening of endophytic bacteria from green betel against four pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Bacillus cereus and Salmonella enteritidis). The number of endophytic bacteria that has been isolated are 14 isolates. Based on the screening results, three isolates of endophytic bacteria have potential activity (characterized by the formation of inhibition zone) against S. aureus. The inhibition zone may indicate that those isolates produce compounds that have antibacterial effects. Those isolates are AS1, BS1 and BS2. The biggest inhibition zone showed by BS1, so it can be concluded that BS1 is the most potential isolate as a novel source of antibacterial compound.

scholarly journals Antibacterial Activity of Rhamnus prinoides extracts against Staphylococcus aureus and Escherichia coli

2019 ◽  
Author(s):  
KINDU GETA
Keyword(s):  
Antibacterial Activity ◽  
Infectious Diseases ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Domestic Animal ◽  
Diffusion Method ◽  
Active Components ◽  
Microbial Infections ◽  
Ethanol Extracts

Abstract Background: Infectious diseases caused by bacteria have a large impact on public health. Increasing in resistant microbial infections intensified the search for new, safer, and more efficacious agents to combat serious microbial infections. Plants have played a central part in combating many diseases in human and domestic animal in many local communities, including Africa. Rhamnus prinoides is Ethiopian medicinal plants that have been used traditionally for the treatment of diverse infectious diseases. Therefore, this research was carried out to evaluate antibacterial activity Rhamnus prinoides extracts against pathogenic bacteria. Methods: The antimicrobial activity tests were carried out by the agar well diffusion method. Broth dilution and streak plate methods were used to determine MIC and MBC of extracts respectively. Results: The results revealed that ethanol extracts of fruits showed the highest antibacterial activity against standard strains of S.aureus with mean inhibition zone of 26±0.58, 28.33±1.2 and 33.33±0.9mm at 25, 50 and 100mg/ml respectively. The lowest mean MIC value (1.04±0.26 mg/ml) was recorded with ethanol extract of fruits against standard strains of S. aureus and the lowest mean MBC value (2.08±0.52 mg/ml) was recorded with ethanol extract of fruits against standard strains and clinical isolates of S. aureu. Conclusion: On the basis of the current findings, Rhamnus prinoides could be a good candidate in the search for new antibacterial agents from natural products against bacterial pathogens. Therefore, further studies are needed to study their toxicology and isolate the bio- active components from this plant.

scholarly journals Citrus Essential Oils’ Antibacterial Effects against Food-Borne Bacteria

2020 ◽  
Vol 6 (3) ◽  
pp. 1-3
Author(s):  
Monique Mancuso ◽  
Renata Zaccone
Keyword(s):  
Growth Inhibition ◽  
Essential Oils ◽  
High Growth ◽  
Antibacterial Activities ◽  
Antibacterial Effects ◽  
Antimicrobial Effects ◽  
Food Borne ◽  
Citrus Essential Oils

Seven citrus essential oils (CEOs) and fourterpenes were tested againstelevenbacterial pathogenic strains isolated from food to evaluate the antimicrobial effects. All CEOs presented antibacterial activities. In particular, CEOs were very active against StaphylococcusEpidermidis, which was found to be the most sensitive toward all oils, showing high growth inhibition.

scholarly journals Assessments of Antibacterial Effects of Aqueous-Ethanolic Extracts of Sida rhombifolia’s Aerial Part

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Demeke Debalke ◽  
Mastewal Birhan ◽  
Amebaye Kinubeh ◽  
Muluken Yayeh
Keyword(s):  
Antibacterial Activity ◽  
Solvent Extraction ◽  
Infectious Diseases ◽  
Plant Extract ◽  
Aerial Part ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Extraction Methods ◽  
Diffusion Method ◽  
Tetrazolium Chloride

Infectious diseases are the critical problems of the world as a result of the emergence of different antimicrobial resistant microorganisms due to several reasons like misuses and repeating uses of antibiotics. Because of this, searching of new treatment method is important from natural substances to against those infectious diseases in both human and animals’ aspects. Among those plants, Sida rhombifolia has various roles against those infectious diseases through its different phytochemical components. The objectives of this study were assessing the antibacterial activity of the aqueous-methanol extract of the plant’s aerial part and knowing the phytochemical constituents of the plant. Preliminary phytochemical screening revealed that the extract of S. rhombifolia’s aerial part possesses flavonoids, alkaloids, polyphenols, and quinines. In addition to this, the antibacterial activity of the plant extract was evaluated on five pathogenic bacteria species using agar well diffusion method at different concentrations of plant extracts. Minimum inhibition concentration and minimum bactericidal concentration determinations were done by tetrazolium chloride microtiter dilution assay. The inhibition zone of mean diameters ranging from 0.00 to 7.67mm against all test bacteria was significantly (p<0.05) much less than that of the positive control Chloroamphinicole (30μg/disc) with the range of 14.33mm-15mm of inhibition zone of diameters. The inhibition zones of the tested bacteria at the concentration of 62.5mg/ml were much less than the higher concentration (500mg/ml) and significantly different (p<0.05), whereas the MIC value ranges from 4.62 to 97.22mg/ml and the MBC value ranges from 4.62 to 125.00mg/ml. Even if the plant extract showed antibacterial activity, it was lower than that of other solvent extraction methods; so other solvent extraction methods and fractionates must be conducted to investigate the antibacterial activities of the plant extract on different bacterial strains and species that cause different diseases.

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