Isolation and Identification of Andrographolide Compounds from the Leaves of Sambiloto Plant (Andrographis paniculata Ness)

2021 ◽  
Vol 4 (1) ◽  
pp. 108
Author(s):  
Devi Ayu Septiani ◽  
Aliefman Hakim ◽  
Lalu Raftha Patech ◽  
Zulhalifah Zulhalifah ◽  
Siswadi Siswadi
Keyword(s):  
Infrared Spectroscopy ◽  
Respiratory Tract ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Andrographis Paniculata ◽  
Pharmacological Effects ◽  
Isolation And Identification ◽  
Qualitative Test ◽  
Ft Ir ◽  
Anti Inflammatory

Sambiloto plant (Andrographis paniculata Ness) is a plant that has been used as medicine from generation to generation. Bioactive compounds in Sambiloto have pharmacological effects such as immunostimulants (increase immunity), antibiotic diuretics (facilitate urine), antipyretics, anti-inflammatory (anti-inflammatory), hepatoprotective, hypotensive, hypoglycemic, antibacterial, anti-inflammatory, respiratory tract, and heart and lung meridians - lungs. The bioactive compound in Sambiloto which is mostly found in the leaves is Andrographolide. In this study, the isolation of Andrographolide from the leaves of the sambiloto plant (Andrographis paniculata Ness) was carried out using purification and crystallization methods aimed at obtaining pure Andrographolide isolates more efficiently and identifying the results of Andrographolide isolates. The results showed that the isolates obtained using the purification and crystallization methods obtained a yield of 0.47%. In the qualitative test of Andrographolide isolates using eluent and acetate: n-hexane (3: 2), the Rf value was 0.38. The results obtained from Andrographolide isolates using infrared spectroscopy (FT-IR) were identical to the literature on Andrographolide. The absorption peaks at the wavenumbers obtained includes 3400,41 cm-1, 1979,68 cm-1, 1959,46 cm-1, 2928,22 cm-1, 1727,56 cm-1, 1646,98 cm-1, and 907,53 cm-1.

scholarly journals Extraction, Phytochemical Screening, Isolation and Identification of Bioactive Compounds from Extract of the plant Euphorbia Thymifolia Linn

2019 ◽  
Vol 9 (3) ◽  
pp. 107-113
Author(s):  
Rashmi Shrivastava ◽  
Jyotsana Mishra
Keyword(s):  
Bioactive Compounds ◽  
13C Nmr ◽  
Skin Diseases ◽  
Bioactive Compound ◽  
Phytochemical Analysis ◽  
Isolation And Identification ◽  
Test Protocol ◽  
Isolation And Characterization ◽  
Ft Ir ◽  
Pharmaceutical Industries

Medicinal plants play an important role in the development of potent therapeutic agents. Plant based drugs provide outstanding contribution to modern therapeutics as a source of many valuable secondary metabolites which serves as plant defense mechanisms against predator such as microorganism, insects and herbivores which have been proved to be potentially active compounds. Euphorbia Thymifolia Linn (E. Thymifolia) is commonly known as ‘duddi’ or in Sanskrit means Laghu didhika or Raktavindaka. It belongs to the family Euphorbiceae. This plant is bitter, acrid, sweet and used as thermogenic, laxative and diuretic. This plant is widely used in the ayurveda to cure many diseases like vitiated condition of constipation, helminthiasis and ringworm skin diseases and leprosy. The aim of the present study is to examine E.Thymifolia Linn whole plant for phytochemical profile, Isolation and Identification of bioactive compounds. Qualitative analysis of various phytochemical constituents was determined by the well-known test protocol available in the literature. Isolation and characterization of bioactive compound from methanolic extract of E. Thymifolia has been conducted. The bioactive compound from methanolic extracts was isolated by several processes, such as TLC, column chromatography and preparative TLC. The isolated bioactive compound is identified by UV-Vis spectrophotometer, FT-IR, 1H, 13C-NMR and Mass. The obtained compound is continued to the preparative TLC using chloroform: methanol (50:50, v/v) as eluent. The UV-Vis spectrum showed one peaks of maximum absorbance at 312.8nm. Then, the FT-IR spectrum showed several peaks that confirmed the presence of functional group of derivative of compound, i.e. 669.05, 928.58, 1070.85, 1215.51 and 1710.07cm-1. 1H and 13C-NMR spectrum confirmed the bioactive compound present in plant. Phytochemical analysis revealed the presence of alkaloids, glycosides, phenols, flavonoids, tannins. The findings of the present study will be helpful to phytochemists, pharmacologists and pharmaceutical industries. Keywords: Euphorbia Thymifolia, Qualitative phytochemical, Isolation, Bioactive compounds

EXTRACTION AND EVALUATION OF ANTI INFLAMMATORY ACTIVITY OF SPATHIPHYLLUM CANNIFOLIUM

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Raha Ahmad Raus ◽  
Norhayati Abdullah ◽  
Nurul Fakhriah Ismail ◽  
Munira Shahbuddin
Keyword(s):  
Optimum Condition ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Inflammatory Activity ◽  
Chloroform Fraction ◽  
Landscape Plant ◽  
Anti Cancer ◽  
Anti Inflammatory ◽  
Face Centered ◽  
Central Composite

Sphathiphyllum cannifolium is abundantly planted in Malaysia as a landscape plant. However, this plant also has medical benefit to human as it was previously found to possess anti-bacteria and anti-cancer activities. In the present study, the plant was evaluated for its anti inflammatory activity and the optimum condition for the extraction of bioactive compounds with anti inflammatory activity was determined. Semi purification of the compounds possessing anti inflammatory activity was conducted as well. To extract the bioactive compound, sonication with methanol as a solvent extraction was carried out. Face-Centered Central Composite Design (FCCCD) and Response Surface Methodology (RSM) were used to design and identify the optimum condition to extract the compound, respectively. The results showed that the plant was positive for the anti inflammatory activity with IC50 6.3 µg/ml. Sonication for 60 min at 40°C using medium frequency was the optimum condition to carry out the extraction for bioactive compounds. After semi purification of the initial crude extract by solvent partitioning, chloroform fraction was found to be positive for anti inflammatory activity. In conclusion, this study suggested that S. cannifolium possess anti inflammatory activity and should be further analyzed to identify the specific compounds that responsible for this activity.   

Naringenin: a potential bioactive compound and pathways of biosynthesis – a review

2021 ◽  
pp. 13-20
Keyword(s):  
Secondary Metabolites ◽  
Natural Compound ◽  
Higher Plants ◽  
Bioactive Compound ◽  
Effective Drug ◽  
Pharmacological Effects ◽  
Human Diet ◽  
Anti Cancer ◽  
Anti Inflammatory ◽  
Plant Metabolite

Naringenin is a member of the flavonoid family. This natural compound represents a large proportion of secondary metabolites produced by higher plants and is a rich part of the human diet. Naringenin also has been used in the pharmaceutical and medical fields as an effective drug for anti-oxidative, anti-cancer, anti-obesity, and anti-inflammatory activities. Naringenin is also a typical plant metabolite, that has never been reported to be produced in prokaryotes. Recently, many papers reported that various members of the Streptomyces family, a genus of actinobacteria, had a novel pathway to produce naringenin. As a result, this review focuses on some clinical pharmacological effects and promising applications in the medical of naringenin, also its pathways of biosynthesis.

scholarly journals Antioxidant, Anti-Inflammatory, and Antidiabetic Activities of Bioactive Compounds from the Fruits of Livistona chinensis Based on Network Pharmacology Prediction

2021 ◽  
Vol 2021 ◽  
pp. 1-21
Author(s):  
Yuwei Wang ◽  
Jianxiu Zhai ◽  
Dan Yang ◽  
Na Han ◽  
Zhe Liu ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Inhibitory Activity ◽  
Active Sites ◽  
Network Pharmacology ◽  
Isolation And Identification ◽  
Insulin Resistant ◽  
Ic50 Values ◽  
Catalytic Active Sites ◽  
Anti Inflammatory ◽  
Livistona Chinensis

In this study, a chemical investigation on the fruits of Livistona chinensis (FLC) led to the isolation and identification of 45 polyphenols and 5 alkaloids, including two new compounds (Livischinol (1) and Livischinine A (46)), an undescribed compound (47) and 47 known compounds. FLC was predicted with novel potential antidiabetic function by collecting and analyzing the potential targets of the ingredients. Compound 32 exhibited significant α-glucosidase inhibitory activity ( I C 50 = 5.71  μM) and 1, 6, and 44 showed the PTP1B inhibitory activity with IC50 values of 9.41-22.19 μM, while that of oleanolic acid was 28.58 μM. The competitive inhibitors of PTP1B (compounds 1 and 44) formed strong binding affinity, with catalytic active sites, proved by kinetic analysis, fluorescence spectra measurements, and computational simulations, and stimulated glucose uptake in the insulin-resistant HepG2 cells at the dose of 50 μM. In addition, FLC was rich in antioxidant and anti-inflammatory bioactive compounds so that they could be developed as nutraceuticals against diabetes.

scholarly journals Anti-Inflammatory Activity of an Indole Alkaloid Isolated from Bebuas (Premna serratifolia)

2021 ◽  
Vol 10 (2) ◽  
pp. 84-103
Author(s):  
Muhaimin Muhaimin ◽  
◽  
Muhammad Irhash Shalihin ◽  
Madyawati Latief ◽  
◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Traditional Medicine ◽  
Bioactive Compounds ◽  
Indole Alkaloid ◽  
Ethanol Extract ◽  
Yellow Crystal ◽  
Strong Activity ◽  
Physical Form ◽  
Ft Ir ◽  
Anti Inflammatory

Premna serratifolia known as bebuas has long been used by Malay comunity for both food and traditional medicine. The most notable advantage of this plant is to heal woman after childbirth and to raise the notion that contains anti-inflammatory bioactive compounds. In this study, the hexane extract of bebuas was only contained steroids. Meanwhile the ethanol extract contained various secondary metabolites such as phenolics, alkaloids, flavonoids, steroids and saponins. The ethanol extract was further separated since the targeted compound isolate was an alkaloid. The isolate obtained was a yellow crystal which transformed to yellow oil after being exposed to air. The anti-inflammatory evaluation of the compound gave a result with a strong activity with ED50 = 4.06 mg/KgBW. Characterization by UV-Vis and FT-IR showed that the isolate’s spectra pattern had similarities with bufotenine. It revealed that the isolate shared the same basic skeleton with the bufotenine, especially the indole skeleton. Furthermore, it also shared the same physical form with bufotenine. These evidences strengthened the assumption that the isolate was an indole alkaloid.

scholarly journals Anti-inflammatory Activity of Sclerotium stipitatum Berk. et. Curr. an Ethnomedicinal Fungus, in Chronic and Acute Animal Models of Inflammation

2021 ◽  
pp. 209-218
Author(s):  
Alina K. Sebastian ◽  
P. V. Anto
Keyword(s):  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Isolation And Identification ◽  
Standard Drug ◽  
Significant Inhibitory Effect ◽  
Anti Inflammatory

Sclerotium stipitatum Berk. et. Curr., locally known as ‘nilamanga’ is a rare macro fungus, traditionally used to treat numerous diseases like arthritis, earache, jaundice etc. The present study aims to evaluate the anti-inflammatory activity of ethanol extract of S. stipitatum and identify the bioactive compounds present in them. Phytochemical screening of extracts obtained using different solvents like petroleum ether, chloroform, ethanol and water were done. The best extract was chosen for the acute carrageenan-induced and chronic formalin-induced anti-inflammatory studies. Diclofenac was used as the standard drug. Ethanol extract showed significant inhibition of inflammation induced by carrageenan and formalin-induced paw edema models compared to the control. GC-MS analysis shows certain bioactive compounds. The significant inhibitory effect on paw edema proves that S. stipitatum possesses remarkable anti-inflammatory activity, and isolation and identification of bioactive compounds can be used for new drug formulations.

scholarly journals Isolation and Characterization of Bioactive Anti-inflammatory Flavonoids from Arisaema tortuosum Tubers

2020 ◽  
Vol 32 (12) ◽  
pp. 3093-3096
Author(s):  
Priyanka Chakraborty ◽  
Kamalika Mazumder ◽  
Nripendra Nath Bala ◽  
Sudipta Das
Keyword(s):  
13C Nmr ◽  
Methanolic Extract ◽  
Bioactive Compound ◽  
Inflammatory Activity ◽  
Methanolic Extracts ◽  
Isolation And Characterization ◽  
Ft Ir ◽  
Anti Inflammatory

Present study was focused on the isolation and characterization of the anti-inflammatory compound(s) from the methanolic extracts of Arisaema tortuosum tuber by column chromatography, TLC, FT-IR, 1H, 13C NMR and HR-MS. Among all the fractions obtained A. tortuosum fraction 3 (PC-1) and fraction 5 (PC-2) showed significant in vivo anti-inflammatory activity. This study endorsed that the isolated bioactive compound PC-2 showed better anti-inflammatory activity at a dose of 25 mg/Kg BW. The results indicate that PC-2 and PC-1 methanolic extract of A. tortuosum tuber may be competing for the tent in the therapy of inflammatory ailments.

Milk acetone determination by the photometrical method after microdiffusion and via FT infra-red spectroscopy

2011 ◽  
Vol 28 (1) ◽  
pp. 33-48 ◽  
Author(s):  
Oto Hanuš ◽  
Václava Genčurová ◽  
Yunhai Zhang ◽  
Pavel Hering ◽  
Jaroslav Kopecký ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Indirect Method ◽  
Geometric Mean ◽  
Mid Infrared ◽  
Subclinical Ketosis ◽  
Infra Red ◽  
Variation Range ◽  
Ft Ir ◽  
Infrared Method ◽  
Photometrical Method

Milk acetone determination by the photometrical method after microdiffusion and via FT infra-red spectroscopyMilk acetone (AC) and betahydroxybutyrate (BHB) are important indicators of the energy metabolism of cows (ketosis occurrence) and an effective method for their determination, with reliable results, is of great importance. The goal of this work was to investigate the infrared method MIR-FT in terms of its calibration for milk AC and to develop a usable procedure. The microdiffusion photometric (485 nm; Spekol 11) method was used with salicylaldehyde as a reference (Re) and mid infrared spectroscopy FT (MIR-FT: Lactoscope FT-IR, Delta; MilkoScan FT 6000, M-Sc) as an indirect method. The acetone addition to milk had no recovery using MIR-FT (Delta). The reference AC set must have acceptable statistics for good MIR-FT calibration (M-Sc) and they were: 10.1 ± 9.74 at a geometric mean of 7.26 mg l-1, and a variation range from 1.98 to 33.66 mg l-1. The AC correlation between Re and MIR-FT (Delta) was low at 0.32 (P>0.05 but the Log AC relationship between Re and MIR-FT (M-Sc) was markedly better at 0.80 (P<0.01). The conversion of >10 mg l-1 as an AC subclinical ketosis limit could be > -0.80 (feedback 0.158 mmol l-1 = 9.25 mg l-1) and > -1.66. This could be important for ketosis monitoring (using M-Sc).

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