Phylogenetic Analysis of Rasbora spp. Based on the Mitochondrial DNA COI gene in Harapan Forest

Harapan forest is the remaining lowland tropical forest in Sumatra which represents about 20 percent of the biodiversity on the island of Sumatra. There are several Rasbora species found in the Sungai Kapas Tengah River Refuge in the Harapan Jambi Forest that the relationship is not yet known.This research aims to know kinship and genetic distance several species of Rasbora from Sungai Kapas Tengah, Hutan Harapan Jambi. The method in this research using secondary data from the NCBI website ((National Center for Biotechnology Information). The data taken, namelynucleotide sequence from the Cytochrome Oxidase I (COI) gene in mitochondrial DNA. The Rasbora species analyzed were Rasbora from identification results in the Sungai Kapas Tengah River Refuge, Harapan Jambi Forest, consisting of, Rasbora bankanensis, R. caudimaculata, R. cf. sumatrana, R. dusonensis, R. elegans, R. sumatrana, and R. trilineata. Based on phylogenetic analysis, the location of the branch length in each Rasbora species, the closest kinship is owned byR. sumatrana and R. elegans species. Based on the results of genetic distance analysis, the closest genetic distance was the species R. elegans and R. sumatrana, with a distance value of 0.023 (2.3%). While the farthest genetic distance between R. bankanensis and R. caudimaculata, with a distance value of 0.172 (17.2%).Based on research results It can be concluded that R. bankanensis has a greater kinship and genetic distance value than other Rasbora species, so that this species forms a separate group. Meanwhile, 5 other species have kinship and the value of close genetic distance so that these species are united in the same group. For future researchers, it is hoped that some additional families of fish species will be analyzed for phylogenetic analysis in Sungai Kapas, Hutan Harapan Jambi, so that they can find out the relationship of several other species.

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Multiple Origins of a Mitochondrial Mutation Conferring Deafness

Genetics ◽

10.1093/genetics/145.3.771 ◽

1997 ◽

Vol 145 (3) ◽

pp. 771-776 ◽

Cited By ~ 1

Author(s):

Timothy P Hutchin ◽

Gino A Cortopassi

Keyword(s):

Phylogenetic Analysis ◽

Genetic Distance ◽

Ribosomal Subunit ◽

Sensorineural Deafness ◽

Nucleotide Substitutions ◽

Mitochondrial Mutation ◽

Distance Analysis ◽

Multiple Origins ◽

Mtdna Haplotypes ◽

Mtdna Sequence

A point mutation (1555G) in the smaller ribosomal subunit of the mitochondrial DNA (mtDNA) has been associated with maternally inherited traits of hypersensitivity to streptomycin and sensorineural deafness in a number of families from China, Japan, Israel, and Africa. To determine whether this distribution was the result of a single or multiple mutational events, we carried out genetic distance analysis and phylogenetic analysis of 10 independent mtDNA D-loop sequences from Africa and Asia. The mtDNA sequence diversity was high (2.21%). Phylogenetic analysis assigned 1555G-bearing haplotypes at very divergent points in the human mtDNA evolutionary tree, and the 1555G mutations occur in many cases on race-specific mtDNA haplotypes, both facts are inconsistent with a recent introgression of the mutation into these races. The simplest interpretation of the available data is that there have been multiple origins of the 1555G mutation. The genetic distance among mtDNAs bearing the pathogenic 1555G mutation is much larger than among mtDNAs bearing either evolutionarily neutral or weakly deleterious nucleotide substitutions (such as the 4336G mutation). These results are consistent with the view that pathogenic mtDNA haplotypes such as 1555G arise on disparate mtDNA lineages which because of negative natural selection leave relatively few related descendants. The co-existence of the same mutation with deafness in individuals with very different nuclear and mitochondrial genetic backgrounds confirms the pathogenicity of the 1555G mutation.

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Entomophilic nematodes, Diploscapter coronatus and Oscheius tipulae from Afghanistan

Zootaxa ◽

10.11646/zootaxa.4926.3.5 ◽

2021 ◽

Vol 4926 (3) ◽

pp. 401-416

Author(s):

MOHAMMAD HUSSAIN FALAHZADAH ◽

EBRAHIM SHOKOOHI ◽

GHOLAM HOSSEIN MORAVEJ ◽

PHATU WILLIAM MASHELA ◽

ABDUL KHALID MADADI ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Genetic Distance ◽

Galleria Mellonella ◽

Morphological Characters ◽

Molecular Data ◽

Soil Samples ◽

The Body ◽

Morphometric Data ◽

Molecular Approaches ◽

The Relationship

Several soil samples from different habitats in Badakhshan province of Afghanistan were collected to isolate and characterize bacteria feeding nematodes. The Galleria mellonella-baiting method was used for the isolation of the Afghan insect-associated nematodes. The nematodes were studied using morphological and morphometric data. The Oscheius specimen was characterized by a longer body (630–820 µm) and shorter pharynx (125–145 µm), whereas other morphological characters were not unusual. The Diploscapter specimen had an annulated cuticle, with lip region width 1.5 times shorter than the stoma, and had separated pharyngeal corpus from the isthmus and vulva located in the middle of the body. The molecular data were derived using three loci; 18S, 28S (D2/D3 segment), and ITS rRNA region, which were utilized to measure the genetic distance. The phylogenetic analysis was conducted to reconstruct the relationship tree. Both morphological and molecular approaches confirmed the identity of nematode isolates as Oscheius tipulae and Diploscapter coronatus. This is the first report of insect-associated nematodes from the soil of Afghanistan. Both species were capable of infecting and killing G. mellonella larvae in less than 96 h.

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The Relationship of age, attitude, knowladge, cost to cataract surgery

Jurnal Berkala Epidemiologi ◽

10.20473/jbe.v4i2.2016.176-187 ◽

2017 ◽

Vol 4 (2) ◽

pp. 176

Author(s):

Aminatul Fitria

Keyword(s):

Cataract Surgery ◽

Public Health Problem ◽

Secondary Data ◽

Simple Random Sampling ◽

Operating Costs ◽

Chi Square ◽

Cross Sectional ◽

Age Attitudes ◽

Relationship Of ◽

The Relationship

Cataract is the leading cause of 51% blindness case in the world. Cataract can only be cured trough surgery, but most people with cataract in Indonesia is not in undergoing surgery due to several factors. The increasing number of cataract victim whose not undergoing any treatment to cure them will resulting in increasing number of blindness case, so blindness cause by cataracts can be a public health problem. This research was conducted to determine the relationship of age, attitudes, knowledge and the cost of the action to perform cataract surgery. This research was an observational analytic study with cross sectional design. The samples were cataract patients in Undaan Eye Hospital Surabaya who were randomly selected using a simple random sampling based on medical records of 60 people. Data collection was done by taking secondary data and interviews to patients. Those variables was analyzed with chi square or Fisher’s exact with significancy level at 95%. The result showed that there were correlation between knowledge (p = 0.017), operating costs (p = 0.001) and attitude (0.000) while age was not related (p = 1.000), the actions to perform cataract surgery. The conclusion from this research was the attitude, knowledge and operating costs related to the actions to perform cataract surgery, while age was not related to the actions to perform cataract surgery. It is recommended to give through leaflets or other media in the lobby for improving patient education, counseling to the patient family, the doctor’s advice to convince patient for surgery.Keywords: practice, surgery, cataract, attitudes, costs

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The male sterility-associated pcf gene and the normal atp9-1 gene in Petunia are located on different mitochondrial DNA molecules.

Genetics ◽

10.1093/genetics/129.3.885 ◽

1991 ◽

Vol 129 (3) ◽

pp. 885-895

Author(s):

O Folkerts ◽

M R Hanson

Keyword(s):

Mitochondrial Dna ◽

Male Sterility ◽

Nadh Dehydrogenase ◽

Ribosomal Subunit ◽

Cms Line ◽

Ribosomal Subunit Protein ◽

Mtdna Organization ◽

Relationship Of ◽

The Relationship ◽

Functional Copy

Abstract A mitochondrial DNA (mtDNA) region termed the S-pcf locus has previously been correlated with cytoplasmic male sterility (CMS) in Petunia. In order to understand the relationship of the S-pcf locus to homologous sequences found elsewhere in mtDNAs of both CMS and fertile lines, the structure of the mitochondrial genome of CMS Petunia line 3688 was determined by cosmid walking. The S-pcf locus, which includes the only copies of genes for NADH dehydrogenase subunit 3 (nad3) and small ribosomal subunit protein 12 (rps12) was found to be located on a circular map of 396 kb, while a second almost identical circular map of 407 kb carries the only copies of the genes for 18S and 5S rRNA (rrn18 and rrn5), the only copy of a conserved unidentified gene (orf25), and the only known functional copy of atp9. Three different copies of a recombination repeat were found in six genomic environments, predicting sub-genomic circles of 277, 266 and 130 kb. The ratio of atp9 to S-pcf mtDNA sequences was approximately 1.5 to 1, indicating that sub-genomic molecules carrying these genes differ in abundance. Comparison of the mtDNA organization of the CMS line with that of the master circle of fertile Petunia line 3704 reveals numerous changes in order and orientation of ten different sectors.

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IMPACT OF DIVIDEND PAYOUT ON FIRM’S PERFORMANCE UNDER LOW GROWTH OPPORTUNITIES

Humanities & Social Sciences Reviews ◽

10.18510/hssr.2021.93114 ◽

2021 ◽

Vol 9 (3) ◽

pp. 1156-1165

Author(s):

Taymoor Ali ◽

Muhammad Kashif Khurshid ◽

Adnan Ali Chaudhary

Keyword(s):

Stock Exchange ◽

Manufacturing Sector ◽

Secondary Data ◽

Financial Statements ◽

Manufacturing Firms ◽

Growth Opportunities ◽

Dividend Payout ◽

Relationship Of ◽

The Relationship

Purpose of the study: The objective of the study was to investigate the relationship of the dividend payout on a firm's performance under low growth opportunities from the manufacturing sector of Pakistan. Methodology: A sample of 251 firms out of 378 manufacturing firms listed at the Pakistan Stock Exchange (PSX), have been carefully chosen for the era of ten years from 2006 to 2015. The secondary data was obtained from the firm’s web financials and analysis of financial statements, published by the statistics department of the State Bank of Pakistan. For the persistence of investigation panel data (fixed effect) analyses were employed in this study. Main Findings: The fallouts of the analysis revealed that the dividend payout ratio has an insignificant relationship with the firm's performance in the low growth perspectives of the study. Applications of this study: The findings of the study are helpful for the financial managers of the firms facing low growth opportunities. Furthermore, the investors in capital markets can use the findings of this while investing. The originality of this study: The study focussed on the role of low growth opportunities while studying the nexus of dividend pay-out and the firm’s financial performance which inherits the novelty and originality of the study.

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Ecossistemas de inovação das universidades federais brasileiras: um mapeamento dos núcleos de inovação tecnológica, incubadoras de empresas de base tecnológica e parques tecnológicos

International Journal of Innovation ◽

10.5585/iji.v7i3.66 ◽

2019 ◽

Vol 7 (3) ◽

pp. 341-358

Author(s):

Rafael Morais Pereira ◽

Humberto Rodrigues Marques ◽

Rodrigo Gava

Keyword(s):

Technological Innovation ◽

Linear Regression Analysis ◽

Secondary Data ◽

Innovation Systems ◽

Simple Linear Regression ◽

Technological Structure ◽

Technology Parks ◽

Relationship Of ◽

The Relationship

Technological innovation as an engine for development requires a structural apparatus to its consolidation, reinforcing the approach of innovation systems, with emphasis on the role of universities. Therefore, considering that associated with this potential are present several innovative elements, such as the Technological Innovation Centers (NITs), the incubators of technology-based companies (IEBTs) and Technology Parks (ParqTecs), and given the lack of systematization National these instruments, the ultimate goal of this study was to analyze the environment for innovation in Brazilian federal universities, through the mapping of NITs, the IEBTs and ParqTecs associated with these institutions, further outlining the relationship of these instruments with technological variables (technological scholarships and patents). We used a qualitative and quantitative approach, descriptive nature, with collection of secondary data on institutional sites of universities and the adoption of simple linear regression analysis. The results realized all 63 Brazilian federal universities and revealed that the most widespread instrument between universities are the NITs, since its presence in 86% of the analyzed institutions. Have business incubators, idealized to strengthen interaction in innovation systems, are associated with 68% of universities, enabling regional development contexts. In relation to technology parks, it was found that, depending on the expenditure required for its implementation, only 26 universities are integrated into ParqTecs. The contributions of this study are concentrated in addition to the mapping done in highlighting the distinctions between the Brazilian regions in terms of scientific and technological structure and the importance of innovation ecosystems.

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Efisiensi belanja administrasi pemerintah Kota Palembang: Pendekatan Stochastic Frontier Analysis

Jurnal Ekonomi Pembangunan ◽

10.29259/jep.v15i2.8833 ◽

2019 ◽

Vol 15 (2) ◽

pp. 85-91

Author(s):

Ririn Puji Astuti ◽

M. Syirod Saleh ◽

Muhammad Subardin

Keyword(s):

Stochastic Frontier Analysis ◽

Capital Expenditure ◽

Stochastic Frontier ◽

Secondary Data ◽

Regional Government ◽

Frontier Analysis ◽

Local Revenue ◽

Relationship Of ◽

The Relationship ◽

The City

This study aims to find out the efficiency of the administration of Palembang City, 2002 – 2016 period in building the area with and components that caused whether or not administrative expenditure of an area was efficient. This study uses data secondary data that is realization data of administrative expenditure, average employe’s expenditure, capital expenditure and local revenue. This data was tested by stochastic frontier analysis (SFA). The results of estimation from the stochastic frontier analysis (SFA) found that the administrative expenditure of the city of Palembang was inefficiency. The average variable of employee expenditure has a negative related and has a significant effect to administration costs in Palembang City. The relationship of variable capital expenditure with the administration costs of the regional government in the city of Palembang has a positive and significant effect. And the Local Revenue variables have a positive and significant effect to administration costs in Palembang City.

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Chiridota heheva—the cosmopolitan holothurian

Marine Biodiversity ◽

10.1007/s12526-020-01128-x ◽

2020 ◽

Vol 50 (6) ◽

Author(s):

Elin A. Thomas ◽

Ruoyu Liu ◽

Diva Amon ◽

Jon T. Copley ◽

Adrian G. Glover ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Deep Sea ◽

Species Complex ◽

Hydrothermal Vents ◽

Novel Species ◽

Cold Seeps ◽

Ecological Studies ◽

Chemosynthetic Ecosystems ◽

Relationship Of ◽

The Relationship

AbstractChemosynthetic ecosystems have long been acknowledged as key areas of enrichment for deep-sea life, supporting hundreds of endemic species. Echinoderms are among the most common taxa inhabiting the periphery of chemosynthetic environments, and of these, chiridotid holothurians are often the most frequently observed. Yet, published records of chiridotids in these habitats are often noted only as supplemental information to larger ecological studies and several remain taxonomically unverified. This study therefore aimed to collate and review all known records attributed to Chiridota Eschscholtz, 1829, and to conduct the first phylogenetic analysis into the relationship of these chiridotid holothurians across global chemosynthetic habitats. We show that Chiridota heheva Pawson & Vance, 2004 is a globally widespread, cosmopolitan holothurian that occupies all three types of deep-sea chemosynthetic ecosystem—hydrothermal vents, cold seeps and organic falls—as an organic-enrichment opportunist. Furthermore, we hypothesise that C. heheva may be synonymous with another vent-endemic chiridotid, Chiridota hydrothermica Smirnov et al., 2000, owing to the strong morphological, ecological and biogeographical parallels between the two species, and predict that any chiridotid holothurians subsequently discovered at global reducing environments will belong to this novel species complex. This study highlights the importance of understudied, peripheral taxa, such as holothurians, to provide insights to biogeography, connectivity and speciation at insular deep-sea habitats.

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