scholarly journals SANTALUM ALBUM;

2019 ◽  
Vol 26 (05) ◽  
Author(s):  
Sonia Gull ◽  
Asim Mushtaq ◽  
Muhammad Umer ◽  
Sajid Mehmood
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Phytochemical Analysis ◽  
Santalum Album ◽  
Zone Of Inhibition ◽  
Staph Aureus ◽  
Lemon Grass

Medicinal plants are good alternate of antibiotics against many bacterial as well as other diseases. Santalum album (Sandal) and Cymbopogon (Lemon grass) are two important medicinal plants whose important components were extracted by sequential extraction from non-polar to polar solvents. The study was aimed at finding antibacterial and radical scavenging potential of Santalum album (Sandal) and Cymbopogon (Lemon grass). Study Design: In vitro study. Setting: Department of Biochemistry and Molecular Biology, University of Gujrat, Gujrat. Period: 12 months. Material and Methods: Sequential extracts of Santalum album and Cymbopogon with n-hexane, chloroform, acetone, ethylacetate, ethanol, butanol and water respectively were prepared to evaluate antibacterial activity against Staph aureus (25923), Staph aureus (38541), E.coli (25922), E.coli (35318), Streptococcus pyrogenes (Tc-11-2) and Shigella sonnei (BB-8). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was used to assess antioxidant activity. Results: Ethanolic and acetone extracts of sandal and lemongrass showed significant inhibtory activity against all seven strains. In case of sandal, acetone extract exhibited highest inhibitory activity against Staph aureus (25923) with 17±2 mm zone of inhibition while ethanolic extract of lemon grass showed highest activity with 16.333 ± 1.154mm zone of inhibition against E.Coli (35318). Other solvents including chloroform, n-hexane, ethyl acetate and butanol also showed considerable antibacterial activity, but water extracts of both plants showed no activity. All polarity based extracts of both plants exhibited antioxidant activity, ethanolic extracts of sandal and lemon grass showed highest radical scavenging activity with 84.366 ±1.504% and 83.766 ±4.272% inhibitions respectively. The minimum antioxidant activity was observed for chloroform extracts of sandal and n-hexane extract of lemongrass. Conclusion: we concluded that some plants have good antibacterial and antioxidant potential. Their phytochemical analysis can be carried out to find potent antibacterial and antioxidant compounds. This will be effective in combating bacterial diseases because mostly microbes are developing resistance against currently available antibiotics.

scholarly journals Antibacterial Potential of some Plant Extracts against Escherichia coli, along with its Phytochemical Analysis, Total Phenolic Content, and Antioxidant Properties

2019 ◽  
Vol 5 (02) ◽  
pp. 96-102
Author(s):  
Pooja Singh ◽  
Upma Srivastava
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis

In vitro antibacterial activity of 21 plant methanolic extracts were investigated by disc diffusion method against gram-negative bacteria Escherichia coli. Amongst the extracts tested, the Datura stramonium L. and Ocimum basilicum L. showed significant antibacterial activity against the bacterial pathogen. D. stramonium showed the highest antibacterial activity followed by O. basilicum extract. Phytochemical analysis of extracts showed the presence of alkaloids, tannins, saponins, flavonoids, and phenols. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ABTS analysis of potent extract D. stramonium showed appreciable antioxidant activity. For DPPH and ABTS assay the highest antioxidant activity was observed at 60 mg ml-1 concentration with a percent inhibition of 71.66 and 77.19 with IC50 value 9.71 and 22.16 μg ml-1 respectively. Moreover, the radical scavenging activity of the extract was lower than that observed for the synthetic antioxidants butylated hydroxyanisole (BHA )and butylated hydroxytoluene (BHT). The total phenolic content of D. stramonium extract was found to be 0.592 mg GAE/gm which was assayed using Folin ciocalteau reagent. The results provide evidence that the extract of D. stramonium and O. basilicum can be further recommended in the treatment of the infections caused by E. coli; further D. stramonium is also a potential source of natural antioxidants.

scholarly journals Phytochemical analysis of herbal teas containing caffeic acid

2021 ◽  
Vol 57 (2) ◽  
pp. 76-83
Author(s):  
Mihai TODORAN ◽  
◽  
Liana HANGA ◽  
Daniela BENEDEC ◽  
◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Caffeic Acid ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Content ◽  
Phytochemical Analysis ◽  
Chromatography Method ◽  
Caffeic Acid Derivatives ◽  
Level I

Objectives. The present analysis was to investigate the content of caffeic acid derivatives, antioxidant activity, and the presence of caffeic acid in alcoholic extracts obtained from 16 simple teas from the following medicinal plants: Vaccinium mуrtilluѕ, Camеllia ѕinеnѕiѕ, Coffea arabica, Mеlіssa οffіcіnalіs, Οcіmum basіlіcum, Rοsmarіnus οffіcіnalіs, Salvіa οffіcіnalіs, Ηyssοрus οffіcіnalіs, Αrtеmіsіa absіntһіum, Cynara scοlymus, Calendula officinalis, Cοrіandrum satіvum, Fοеnіculum vulgarе, Carum carvі, Rοsa canіna, Crataеgus mοnοgyna. Materials and methods. The presence of caffeic acid was evaluated using the thin layer chromatography method (TLC). The total content of phenylpropanoids from the medicinal herbs alcoholic extracts was determined using a spectrophotometric method. Outcomes. Results were in the range of 0.046-2.426% caffeic acid derivatives (CAE%). The antioxidant properties were measured using the DPPH radical scavenging mechanism. The results were found to be in the range of 63.93% and 0.38% inhibition level (I%). The greatest antioxidant activity was measured for Camellia sinensis (I = 45.54%), followed by Vaccinium myrtillus (I = 50.289%). Conclusions. In this paper it was shown that these medicinal plants are rich in caffeic acid derivatives with antioxidant action capitalized in the prevention of serious diseases.

Qualitative Phytochemical Analysis in Determination of Antioxidant Activity of Methanolic Extract of Oenothera biennis by GCMS – A Preliminary Research Study

2021 ◽  
pp. 3744-3750
Author(s):  
Afroz Patan ◽  
Saranya M. ◽  
Vignesh S. ◽  
Bharathi A. ◽  
Vikram G. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Plant Extract ◽  
Radical Scavenging Activity ◽  
Research Work ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Compound Identification ◽  
Oenothera Biennis

Introduction and Aim: Oenothera biennis an important medicinal plant which belongs to Onagraceae family. It is used for various medicinal purposes in ayurvedic medicine and herbal remedy. The aim of the present study was to evaluate the antioxidant activity of phenol and flavonoid extract of plant of O. biennis and GC-MS analysis for active compound identification. Materials and Methods: Radical scavenging assay and reduction assay methods were used for antioxidant activity. The antioxidant capacity of methanolic plant extractHP-5 column was used for GCMS analysis. Results: The IC50 of DPPH radical scavenging activity of methanol leaves extract of O. biennis was 31.43µg/mL concentration, IC50value of superoxide radical scavenging activity was 37.71µg/mL concentration. The RC50 of phosphomolybdenum reduction of methanolic plant extract of O. biennis was 49.90µg/mL concentration and the RC50of Fe3+ reduction was 37.25µg/mL concentrations. Antioxidant compounds such as Phenol, 2, 6-bis(1,1-dimethyl)-4-[(4-hydroxy-3,5-dimethylphenyl)methyl]-, Cromaril and Oleic acid were eluted by GCMS analysis. Conclusion: The data showed that the methanolic plant extract of O. biennis has significant antioxidant activity. The flavone compounds identified in GCMS could be responsible for antioxidant activity. Further research work needed to isolate active compounds to kill diseases.

In vitro Antimicrobial and Antioxidant Activity of Immunity BoosterTea Formulation

2020 ◽  
Vol 2 (29) ◽  
pp. 14-19
Author(s):  
Zafar Mehmood
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Radical Scavenging ◽  
Natural Antioxidant ◽  
Zone Of Inhibition ◽  
Test Organisms ◽  
Antimicrobial And Antioxidant Activity ◽  
Dpph Radical Scavenging ◽  
Strong Antimicrobial Activity

Abstract-Currently, there is much growing interest in the use of medicinal plants as modulators of the complex immune system. Through a number of vast researches conducted in the area, it is being explored that many of the phytochemicals in the form of alkaloids,tannins,flavonoids,terpenoids,polysaccharides, lactones, and glycoside products areresponsibletocausealterationsintheimmunomodulatory properties. Keeping in mind, the tremendous potential of the medicinal plants and their derived drugs, this study is undertaken and an immunobooster tea formulation (IMBF-01-20) was designed and in vitroantimicrobial and antioxidant activity was tested. The results showed strong antimicrobial activity of formulation(IMBF-01-20) against Staph.aureuswith diameter of zone of inhibition of 32 mm in methanol extract followed by 20 mm in E.coli. While hexane extract also showing good inhibition of test organisms followed by Ethanol extract.Considering the growing demand of natural antioxidant, IMBF-01-20 was tested for their antioxidant activity using DPPH radical scavenging assay.Antioxidant activity (93.4%) was observed in immunobooster tea formulation and revealed it as a natural antioxidant.

scholarly journals In Vitro Assessment of Selected Medicinal Plants Used by the Bapedi Community in South Africa for Treatment of Bacterial Infections

2018 ◽  
Vol 23 ◽  
pp. 2515690X1876273 ◽  
Author(s):  
Refilwe Given Kudumela ◽  
Peter Masoko
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Bacterial Infections ◽  
Antioxidant Activities ◽  
Scientific Information ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Limpopo Province ◽  
Total Phenolic

There is very little scientific information on indigenous plants used for medicinal purposes. Therefore, the aim of the study was to evaluate the antibacterial and antioxidant activities of the leaves of Commelina africana, Elephantorrhiza elephantina, Dombeya rotundifolia, and the whole plant excluding the roots of Schkuhria pinnata indigenous medicinal plants from the Limpopo Province, which may be used for the treatment in humans infected with bacterial pathogens. The screening of different phytoconstituents using standard methods revealed the presence of terpenoids, flavonoids, tannins, and saponins. The total phenolic, tannin, and flavonoid content were estimated using spectrophotometric methods. D rotundifolia had the highest amounts of phenol (259.00 ± 2.65 mg of gallic acid equivalent [GAE]/g), tannin (330.33 ± 15.63 mg of GAE/g), and flavonoid (19.90 ± 0.75 mg of quercetin equivalent [QE]/g) content, while S pinnata had the least. All the plants had antibacterial activity against Escherichia coli and Pseudomonas aeruginosa with the bioautography method. The microbroth dilution assay revealed antibacterial activity with minimum inhibitory concentration values raining from 0.02 to 2.50 mg/mL. D rotundifolia had the highest antioxidant activity in both the free radical scavenging and ferric reducing power methods. S pinnata and C africana extracts had high antibacterial activity, while D rotundifolia had high antioxidant activity, which made them potential plants for isolation of bioactive compounds and possible application in the pharmaceutical industry.

Anti-microbial, antioxidant potential of Cassia fistula and study their phytochemical assessments

2021 ◽  
Vol 7 (2) ◽  
pp. 130-140
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Cassia Fistula

Cassia fistula (C. fistula) is a flowering plant and a member of Fabaceae family. This study was designed to examine the antibacterial, antioxidant and phytochemical activity of ethanolic extract of C. fistula plant. The microbial inhibitory effect of ethanolic extracts of C. fistula was tested against Gram positive isolates such as Bacillus cereus, Staphylococcus aureus and Gram-negative isolates such as Salmonella typhi, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa using disc diffusion method and well diffusion method. The 25 mg extract of C. fistula leaves (CF-05) showed more zone of inhibition against Salmonella typhi, i.e., (21mm) and in 50 mg extract of CF-13 fruit showed best zone of inhibition against Salmonella typhi, i.e., 17 mm. Qualitative analysis and antioxidant activity at various concentrations was also measured. The phytochemical analysis showed the presence of alkaloids, carbohydrates, fats, tannins, flavonoids, saponins, terpenoids, and sterols. The antioxidant activity in 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity revealed the distinguished antioxidant activity of C. fistula.

scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF LEUCAS ASPERA FLOWERS FROM BIHAR, INDIA.

2018 ◽  
Vol 11 (2) ◽  
pp. 223
Author(s):  
Gulnaaz Sabri ◽  
Vimala Y
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Free Radical ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Free Radical Scavenging Assay

 Objective: The aim of this study was to explicate antibacterial, antifungal, and antioxidant activities of Leucas aspera flowers.Methods: Antibacterial activity was done by agar diffusion method. The ethyl acetate extract of L. aspera flower was evaluated against both Gram-positive and Gram-negative bacteria. Antifungal activity was also done by agar diffusion method. The agar used for antifungal activity was Czapek Dox Agar. Nitric oxide scavenging assay and free radical scavenging assay were used for the antioxidant activity. Griess reagent was used in nitric oxide scavenging assay. 1,1-diphenyl-2-picryl hydrazyl was used in free radical scavenging assay.Results: L. aspera flower extract showed good antibacterial activity with the highest zone of inhibition against Vibrio cholera with 23 mm followed by Bacillus polymyxa showing 20 mm zone of inhibition. The ethyl acetate extract of L. aspera flower showed quite a good results with the highest inhibitory activity against Aspergillus niger with 13 mm zone of inhibition and lowest for Trichoderma viridae with 5 mm zone of inhibition. Antioxidant activity of L. aspera flower extract was done by free radical scavenging assay and nitric oxide scavenging assay. Nitric oxide scavenging assay showed prominent results almost performed equal to standard compound Butylated hydroxyl anisole (BHA) The values for 10 μl of L. aspera extract was 50.27, for the standard (BHA) showed 50.81. L. aspera extract values for 50 μl was 69.73 and for BHA, the values was 77.30. For 100 μl, the extract gave 82.70, and for standard BHA, the reading was 89.73.Conclusion: The results showed that L. aspera flower has broad-spectrum antibacterial activity ranging from 23 to 13 mm zone of inhibition. L. aspera flower has strong antioxidative power on nitric oxide radicals. The medicinal properties of plant species have made an outstanding contribution to the origin and evolution of many traditional herbal therapies.

scholarly journals Evaluation of the Antibacterial and Antioxidant Activity of Mimosa rubicaulis and Reinwardtia indica

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Roshani Gurung ◽  
Sundar Adhikari ◽  
Kalpana Parajuli
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Antibacterial Activity ◽  
Plant Species ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Ic50 Value ◽  
Antibacterial And Antioxidant Activity

Many plants have the property of wound healing, but most of the people are using costly allopathic medicine for the wound. This might be due to lack of awareness about the traditional uses and lack of scientific study of ethnomedicinal plants. So, this study aimed to carry out the antibacterial and antioxidant activity of two medicinal plants which are used traditionally for wound healing activity, i.e.,Mimosa rubicaulis and Reinwardtia indica. Different parts of Mimosa rubicaulis (root, stem, and leaves) and Reinwardtia indica (flower and leaves) were used for the study. Extractions were done by maceration using ethanol as solvent. Antibacterial activity was carried out by the well diffusion method, and antioxidant activities were screened by DPPH radical scavenging and NO scavenging assays. Extract of M. rubicaulis has shown a weak zone of inhibition towards S. aureus and P. vulgaris whereas R. indica has shown no zone of inhibition towards selected bacterial strains. Leaf, root, and stem extracts of M. rubicaulis have shown potent antioxidant activity, i.e., IC50 value of 9.8 µg/ml, 10.19 µg/ml, and, 13.64 µg/ml, respectively. Similarly, leaf extract of R. indica exhibited antioxidant activity with an IC50 value of 19.73 μg/ml. The percentage inhibition of NO radicals of root and stem of M. rubicaulis and leaves of R. indica was 31%, 21%, and 22%, respectively. Out of these two plant species, M. rubicaulis had shown antibacterial activity towards selected microorganism, but antioxidant activity was shown by both plant species. These properties on above mentioned two plant species might help for the development of a marketed formulation for antibacterial and wound-healing agent since wound healing is promoted by antibacterial and antioxidant activities.

scholarly journals EVALUATION OF ANTIOXIDANT COMPOUNDS AND FREE RADICAL SCAVENGING ABILITY OF POMEGRANATE FRUIT PEELS

2016 ◽  
Vol 3 (2) ◽  
pp. 48-51
Author(s):  
Jamuna S ◽  
Sathiskumar S
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antioxidant Compounds ◽  
Phytochemical Analysis ◽  
Fruit Peel ◽  
Ic50 Value ◽  
Antioxidant Agent ◽  
Radical Scavenging Ability ◽  
Pomegranate Fruit

The present study was undertaken to investigate the phytochemical profile and antioxidant activity of pomegranate fruit peels. Qualitative and quantitative phytochemical analyses were made for various solvent extracts of fruit peel of pomegranate and for antioxidant activity, ethanolic extract alone was used.The preliminary phytochemical analysis revealed that higher number of secondary metabolites was found in ethanolic extract of fruit peel than the other solvent extracts. The total phenolics and flavonoids contents of ethanolic fruit peel extract was found to be 246.5 mg GAE/100g extract and 83.95 mg QE/100g extractrespectively. The ethanolic fruit peel extracts unveiled highest scavenging ability by quenching the DPPH free radicals with the IC50 value, 142.90µg/mL. The present study showed that the tested pomegranate peels exhibited strong antioxidant activity. These results suggest that pomegranate fruit peel could be exploited as a potential source of natural antioxidant agent.

scholarly journals Antioxidant and Antibacterial Activities of Four Local Medicinal Plants

2020 ◽  
Vol 30 (2) ◽  
pp. 179-187
Author(s):  
Barna Goswami ◽  
Shamoly Akter ◽  
Nemai Chandra Nandi ◽  
Tanjina Akthar Banu ◽  
Shahina Akter ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Leaf Extracts ◽  
Ic50 Value

Potential antioxidant and antibacterial activity of methanolic, chloroformic and n-hexane leaf extracts of four local important medicinal plants like Ocimum americanum, O. basilicum, O. gratissimum and Centella asiatica was investigated. The methanolic leaf extracts of these plant species exhibited the potent DPPH free radical scavenging activity (IC50 value, 2.67 ± 0.01, 14.17 ± 0.11, 60.22 ± 0.01 and 2.39 ± 0.025 μg/ml, respectively). Methanolic leaf extract of C. asiatica showed strongest antioxidant activity. Chloroformic leaf extracts possessed moderate antioxidant activity (IC50 value of 79.44 ± 0.05, 110.56 ± 0.02, 54.95 ± 0.05, 101.0 ± 1.0 μg/ml, respectively) in all samples. The lowest antioxidant activity was recorded from n-hexane leaf extracts of O. americanum, O. gratissimum, C. asiatica and Ocimum basilicum (IC50 value 147.87 ± 0.06, 378.19 ± 2.65, 104.65 ± 0.39, 467.58 ± 0.52 μg/ml, respectively). Methanolic and chloro-formic leaf extracts showed antibacterial activity against both Gram-positive and Gram-negative pathogenic bacteria, namely Bacillus megaterium, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi. Methanolic leaf extract of O. americanum and chloroformic extract of C. asiatica showed excellent antimicrobial activity. Plant Tissue Cult. & Biotech. 30(2): 179-187, 2020 (December)

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