Antimicrobial activity of antibiotic pastes used in pulp therapy through direct contact with a multispecies biofilm: a pilot study

2020 ◽  
Vol 5 (1) ◽  
pp. 17-23
Author(s):  
Mariana Coutinho Sancas ◽  
Anne Caroline Lunardi de Souza ◽  
Amanda Souza Nunes Monteiro ◽  
Andréa Vaz Braga Pintor ◽  
Maysa Lannes Duarte ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pilot Study ◽  
Direct Contact ◽  
Pulp Therapy ◽  
Multispecies Biofilm

scholarly journals In VitroEvaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials againstEnterococcus faecalis,Candida albicans, andStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Duddi Narendra Nirupama ◽  
Mohan Thomas Nainan ◽  
Rajendran Ramaswamy ◽  
Sethumadhavan Muralidharan ◽  
Hulimangala Hosakote Lingareddy Usha ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Direct Contact ◽  
Microbial Growth ◽  
Antimicrobial Property ◽  
Recurrent Infection ◽  
Antimicrobial Effect ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
Root Canal Sealers ◽  
Direct Contact Test

Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms,E. faecalis, C. albicans, andS. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity againstE. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity againstC. albicansandS. aureuscompared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity againstE. faecalis, C. albicans, andS. aureus.

scholarly journals Antimicrobial Effectiveness of Calcium Silicate Sealers against a Nutrient-Stressed Multispecies Biofilm

2020 ◽  
Vol 9 (9) ◽  
pp. 2722
Author(s):  
Rahul Bose ◽  
Konstantinos Ioannidis ◽  
Federico Foschi ◽  
Abdulaziz Bakhsh ◽  
Robert D. Kelly ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Silicate ◽  
Antimicrobial Properties ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Biofilm Inhibition ◽  
Planktonic Bacteria ◽  
Multispecies Biofilm ◽  
Significant Difference ◽  
Ah Plus

Purpose: This study compared the antimicrobial efficacy of calcium silicate sealers (BioRoot RCS and Total Fill BC) and conventional sealers (AH Plus and Tubli-seal) against planktonic bacteria and a nutrient-stressed multispecies biofilm. Methods: Antimicrobial properties of freshly mixed sealers were investigated using the direct contact test (DCT) and a nutrient-stressed multispecies biofilm comprised of five endodontic strains. Antimicrobial activity was determined using quantitative viable counts and confocal laser scanning microscopy (CLSM) analysis with live/dead staining. The pH of the sealers was analysed over a period of 28 days in Hanks Balanced Salt Solution (HBSS). Analysis of variance (ANOVA) with Tukey tests and the Kruskal–Wallis test were used for data analysis with a significance of 5%. Results: All endodontic sealers exhibited significant antimicrobial activity against planktonic bacteria (p < 0.05). BioRoot RCS caused a significant reduction in viable counts of the biofilms compared to AH Plus and the control (p < 0.05), while no significant difference could be observed compared to TotalFill BC and Tubli-seal (p > 0.05). CLSM analysis showed that BioRoot RCS and TotalFill BC exhibited significant biofilm inhibition compared to Tubli-seal, AH Plus and the control (p < 0.05). BioRoot RCS presented with the highest microbial killing, followed by TotalFill BC and Tubli-seal. Alkalizing activity was seen from the onset by BioRoot RCS, TotalFill BC and AH Plus. After 28 days, BioRoot RCS demonstrated the highest pH in HBSS (pH > 12). Conclusions: Calcium silicate sealers exhibited effective antimicrobial properties. This was demonstrated by superior biofilm inhibition capacity and microbial killing, with strong alkalizing activity compared to epoxy-based and zinc oxide-eugenol-based sealers.

A novel direct contact method for the assessment of the antimicrobial activity of dental cements

2013 ◽  
Vol 93 (3) ◽  
pp. 168-172 ◽  
Author(s):  
E.M. Costa ◽  
S. Silva ◽  
A.R. Madureira ◽  
A. Cardelle-Cobas ◽  
F.K. Tavaria ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Direct Contact ◽  
Contact Method

scholarly journals The Effect of Periogen Solution on Dental Calculus in vitro: A Pilot Study

2017 ◽  
Vol 6 (1) ◽  
pp. 33-34
Author(s):  
Marta Radnai ◽  
Zsolt Rajnics
Keyword(s):  
Pilot Study ◽  
Hydrochloric Acid ◽  
Acid Solution ◽  
Oral Hygiene ◽  
Direct Contact ◽  
Photometric Method ◽  
Distilled Water ◽  
Dental Calculus ◽  
Carbamide Peroxide

ABSTRACT Introduction Calculus can accommodate teeth and prosthetic restorations when the patient's oral hygiene is poor. Hardened calculus cannot be removed by patients, it needs professional cleaning using ultrasonic scaler or hand instrument. Solutions dissolving and preventing accumulation of dental calculus may help to keep dentures clean. The aim of this investigation was to examine the effect of Periogen on dental calculus in vitro. Materials and methods Calculus was collected via scaling from patients during a routine dental checkup. The samples were stored in carbamide peroxide solution (5%) for 24 hours, then rinsed and stored in distilled water. First, the Ca2+ content of the calculus was determined by photometric method after treating with cc. hydrochloric acid solution for 1 hour. The calculus samples were put in Periogen solution, prepared according to manufacturer's instruction for 16 hours, then crushed and put in Periogen solution with the same concentrate for 4.5 hours. The Ca2+ dissolved from calculus was measured using same photometric method. Results Calculus samples contained 26 mg/100 mg Ca2+, which is similar to dentin (27–28 mg/100 mg) and to enamel (36 mg/100 mg). The Ca2+ dissolved from calculus after treating with cc. hydrochloric acid for 1 hour was considered 100%. Ca2+ dissolution was 1.5% after 16 hours (0.09%/h) and 5.45% (1.21%/h) for the next 4.5 hours after pulverizing the sample. This showed Periogen Ca2+ dissolution was 385 µg/100 mg after 16 hours direct contact with the material. Conclusion The experiment showed that pulverizing the previously hard calculus was done easily after soaking it in Periogen. The ability of Periogen to soften the calculus needs to be further investigated. How to cite this article Rajnics Z, Radnai M. The Effect of Periogen Solution on Dental Calculus in vitro: A Pilot Study. Int J Experiment Dent Sci 2017;6(1):33-34.

scholarly journals Altered Blood Levels of Vitamin D, Cathelicidin and Parathyroid Hormone in Patients with Sepsis—a Pilot Study

2017 ◽  
Vol 45 (1) ◽  
pp. 36-45 ◽  
Author(s):  
T. Greulich ◽  
W. Regner ◽  
M. Branscheidt ◽  
C. Herr ◽  
A. R. Koczulla ◽  
...  
Keyword(s):  
Vitamin D ◽  
Antimicrobial Activity ◽  
Parathyroid Hormone ◽  
Pilot Study ◽  
Serum Levels ◽  
Blood Levels ◽  
Healthy Controls ◽  
Icu Patients ◽  
The Impact

It has been recognised that vitamin D (VitD) has a potential role in the regulation of inflammation and protection from infection. In a prospective clinical observational pilot study, we investigated the serum levels of 25-hydroxyvitamin-D3 (25(OH D3), 1,25-hydroxyvitamin-D3 (1,25(OH)2D3), parathyroid hormone (PTH), and cathelicidin in intensive care unit (ICU) patients with or without systemic inflammatory response syndrome (SIRS). We included 32 patients with SIRS (septic patients), 16 ICU patients without SIRS, and 16 healthy controls. To substantiate the findings of the clinical study, we stimulated monocyte-derived macrophages with microbial patterns and analysed the impact of VitD on release of cytokines and antimicrobial activity. We found that patients with or without SIRS had relatively low levels of 25(OH)D3 and 1,25(OH)2D3. Patients with sepsis had significantly lower levels of 25(OH)D3 as compared to ICU control patients and healthy controls (10.53 ± 11.3 μg/l versus 16.46 ± 12.58 μg/l versus 24.04 ± 12.07 μg/l); the same was true for 1,25(OH)2D3. Serum levels of PTH and cathelicidin were significantly increased in sepsis patients, as compared to the other groups. In vitro, VitD significantly decreased the release of pro-inflammatory cytokines from macrophages and increased the antimicrobial activity of the cells. We concluded that patients with sepsis have significantly lower VitD levels. In vitro, VitD modulates inflammation and increases the antibacterial activity of innate immune cells. These findings suggest that VitD insufficiency is mechanistically related to increased susceptibility to SIRS and sepsis.

scholarly journals Antimicrobial and cytotoxic capacity of pyroligneous extracts films of Eucalyptus grandis and chitosan for oral applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Juliana Leitzke Santos de Souza ◽  
Tomaz Alves ◽  
Laísa Camerini ◽  
Fernanda Nedel ◽  
Angela Diniz Campos ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Direct Contact ◽  
Eucalyptus Grandis ◽  
Effective Control ◽  
Natural Polymers ◽  
Contact Test ◽  
Enamel Demineralization ◽  
Chitosan Films ◽  
Direct Contact Test

AbstractChitosan films containing distilled pyroligneous extracts of Eucalyptus grandis (DPEC), characterized and developed by Brazilian Agricultural Research Corporation—Embrapa Temperate Agriculture (EMBRAPA-CPACT), were evaluated for antimicrobial activity against Candida albicans, Streptococcus mutans, and Lactobacillus acidophilus by direct contact test. Further, their capacity for the prevention of teeth enamel demineralization and cytotoxicity in vitro were also determined. The natural polymers were tested at different concentrations (1500–7500 µg mL−1) and the formulation of an experimental fluoride varnish with antimicrobial activity was evaluated by direct contact test, whereas cytotoxicity was analyzed through the colorimetric MTT assay. Preliminary data showed no statistically significant differences in cytotoxicity to NIH/3T3 cell line when DPEC is compared to the control group. On the other hand, the antimicrobial capacity and demineralization effects were found between the test groups at the different concentrations tested. Chitosan films containing distilled pyroligneous extracts of E. grandis may be an effective control strategy to prevent biofilm formation related to dental caries when applied as a protective varnish. They may inhibit the colonization of oral microorganisms and possibly control dental caries through a decrease in pH and impairment of enamel demineralization.

Surface Modification of Natural Rubber Film with PMMA Nanoparticles Stabilized by Chitosan or its Derivative

2014 ◽  
Vol 875-877 ◽  
pp. 59-62
Author(s):  
Thanida Arpornwichanop ◽  
Nuttakun Kanjanathaworn ◽  
Pramuan Tangboriboonrat
Keyword(s):  
Surface Roughness ◽  
Antimicrobial Activity ◽  
Surface Modification ◽  
Natural Rubber ◽  
Methyl Methacrylate ◽  
Direct Contact ◽  
Miniemulsion Polymerization ◽  
Surface Friction ◽  
Layer By Layer ◽  
Rubber Film

Poly(methyl methacrylate) (PMMA) latex particles stabilized by chitosan (CS) or N,N,N-trimethylated chitosan (TMC) (50% degree of quaternization) were prepared via the miniemulsion polymerization. The spherical PMMA-CS nanoparticles (342 nm) were then deposited onto sulphur-prevulcanized natural rubber (SPNR) film via the Layer-by-Layer (LbL) technique. The aims were to reduce the surface friction of rubber film and to avoid the direct contact between SPNR and human skin which might cause the allergic problem for some hypersensitive users. Results confirmed the presence of PMMA-CS particles which caused an increase in the surface roughness of SPNR from 46 to 57. However, the PMMA-CS did not exhibit the antimicrobial activity. Therefore, the antimicrobial activity against E.coli and S.aureus of the SPNR film coated with PMMA-TMC particles is investigated.

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