scholarly journals Evaluation of microbial contamination in a surgical department of a Romanian military emergency hospital - A case study

2021 ◽  
Vol 6 (1) ◽  
pp. 026-028
Author(s):  
Coculescu Bogdan-Ioan ◽  
Coculescusup Elena Claudia
Keyword(s):  
Escherichia Coli ◽  
Bacterial Contamination ◽  
Microbial Contamination ◽  
Maximum Level ◽  
Operating Environment ◽  
Surgical Department ◽  
E Coli ◽  
Emergency Hospital ◽  
Number Of Bacteria

Assessment of the microbial load of the operating environment during daily pre-, intra-, and post-operative procedures in a surgical department of a military emergency hospital in Bucharest showed the bacterial contamination of intra-operative air by increasing the number of bacteria above the allowed maximum level and the detection of a strain of Escherichia coli (E. coli).

scholarly journals Contamination Analysis of Escherichia coli on Broiler Chicken Meat in Traditional Markets of Medan City

2021 ◽  
Vol 9 (1) ◽  
pp. 73-79
Author(s):  
Julinda Siregar ◽  
Sayed Umar ◽  
Nevy Diana Hanafi
Keyword(s):  
Escherichia Coli ◽  
Broiler Chicken ◽  
Bacterial Contamination ◽  
Microbial Contamination ◽  
Sampling Method ◽  
Chicken Meat ◽  
Gram Negative Bacteria ◽  
E Coli ◽  
Traditional Markets ◽  
Maximum Limit

Escherichia coli (E. coli) is a gram-negative bacteria that can reduce meat quality and cause diarrhea if the amount is too much. This study investigates the presence of E. coli contamination in chicken meat sold in traditional markets in Medan. This research was conducted using the purposive sampling method and collected samples from 30% of each nine traditional markets with 32 samples. The traditional markets in Medan, namely Petisah, Central, Sei Kambing, Kampung Lalang, Kwala Bekala, Helvetia, Kampung Baru, Kemiri and Padang Bulan. Research was conducted by using the CFU / ml method with chromocult media. Samples were analyzed in the laboratory of the Regional Veterinary Disease Investigation Center Region 1 Medan. The results showed 32 samples of chicken meat had exceeded the maximum limit of microbial contamination. The highest total microbial average was in the Kampung Lalang market, 3.49 Log CFU / g, and the lowest was in the Kemiri market of 2.79 Log CFU / g. Based on the research results, it was found that the meat in the traditional markets of Medan was all contaminated with E. coli and had passed the maximum limit of bacterial contamination.

scholarly journals A Statistical Model for Assessing Sample Size for Bacterial Colony Selection: A Case Study of Escherichia Coli and Avian Cellulitis

2000 ◽  
Vol 12 (2) ◽  
pp. 118-125 ◽  
Author(s):  
Randall S. Singer ◽  
Wesley O. Johnson ◽  
Joan S. Jeffrey ◽  
Richard P. Chin ◽  
Tim E. Carpenter ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Statistical Model ◽  
Clinical Sample ◽  
Agar Plate ◽  
Sample Sizes ◽  
Simple Task ◽  
Single Strain ◽  
Bacterial Colony ◽  
E Coli

A general problem for microbiologists is determining the number of phenotypically similar colonies growing on an agar plate that must be analyzed in order to be confident of identifying all of the different strains present in the sample. If a specified number of colonies is picked from a plate on which the number of unique strains of bacteria is unknown, assigning a probability of correctly identifying all of the strains present on the plate is not a simple task. With Escherichia coli of avian cellulitis origin as a case study, a statistical model was designed that would delineate sample sizes for efficient and consistent identification of all the strains of phenotypically similar bacteria in a clinical sample. This model enables the microbiologist to calculate the probability that all of the strains contained within the sample are correctly identified and to generate probability-based sample sizes for colony identification. The probability of cellulitis lesions containing a single strain of E. coli was 95.4%. If one E. coli strain is observed out of three colonies randomly selected from a future agar plate, the probability is 98.8% that only one strain is on the plate. These results are specific for this cellulitis E. coli scenario. For systems in which the number of bacterial strains per sample is variable, this model provides a quantitative means by which sample sizes can be determined.

scholarly journals Spread of Antibiotic Resistance in Aquatic Environments: E. coli as a Case Study

Proceedings ◽  
2018 ◽  
Vol 2 (11) ◽  
pp. 693 ◽  
Author(s):  
Maria Adamantia Efstratiou ◽  
Marina Bountouni ◽  
Efthimios Kefalas
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Antibiotic Resistance ◽  
Rural Communities ◽  
Ground Water ◽  
Aquatic Environments ◽  
Multiple Resistance ◽  
E Coli ◽  
Of Greece

The aim of this study was to gather information on the spread of antibiotic resistance in Escherichia coli isolates from wells, boreholes and untreated drinking water in islands of Greece. We analyzed for antibiotic resistance 235 E. coli strains isolated from untreated drinking water of small rural communities, and ground water from 4 islands. Resistance was tested against Norfloxacin, Ciprofloxacin, Levofloxacin, Amoxicillin and Cefaclor. More than half (54.9%) were resistant to at least one of the antibiotics tested. Of these 26.3% showed multiple resistance (to two or more antibiotics). Strains from drinking water sources were overall more sensitive. Frequent resistance was observed for Amoxicillin (38.3%) and Levofloxacin (28.5%), low for Norfloxacin (5.5%).

Survival of Escherichia coli on Lettuce under Field Conditions Encountered in the Northeastern United States

2017 ◽  
Vol 80 (7) ◽  
pp. 1214-1221 ◽  
Author(s):  
Daniel L. Weller ◽  
Jasna Kovac ◽  
Sherry Roof ◽  
David J. Kent ◽  
Jeffrey I. Tokman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Microbial Contamination ◽  
Field Conditions ◽  
Risk Assessments ◽  
Most Probable Number ◽  
Northeastern United States ◽  
Probable Number ◽  
E Coli ◽  
The Relationship ◽  
Sample Time

ABSTRACT Although wildlife intrusion and untreated manure have been associated with microbial contamination of produce, relatively few studies have examined the survival of Escherichia coli on produce under field conditions following contamination (e.g., via splash from wildlife feces). This experimental study was performed to estimate the die-off rate of E. coli on preharvest lettuce following contamination with a fecal slurry. During August 2015, field-grown lettuce was inoculated via pipette with a fecal slurry that was spiked with a three-strain cocktail of rifampin-resistant nonpathogenic E. coli. Ten lettuce heads were harvested at each of 13 time points following inoculation (0, 2.5, 5, and 24 h after inoculation and every 24 h thereafter until day 10). The most probable number (MPN) of E. coli on each lettuce head was determined, and die-off rates were estimated. The relationship between sample time and the log MPN of E. coli per head was modeled using a segmented linear model. This model had a breakpoint at 106 h (95% confidence interval = 69, 142 h) after inoculation, with a daily decrease of 0.70 and 0.19 log MPN for 0 to 106 h and 106 to 240 h following inoculation, respectively. These findings are consistent with die-off rates obtained in similar studies that assessed E. coli survival on produce following irrigation. Overall, these findings provide die-off rates for E. coli on lettuce that can be used in future quantitative risk assessments.

scholarly journals Occurrence of Escherichia coli in meat preparations

Meso ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. 401-404
Author(s):  
Ana Robina ◽  
Lidija Kozačinski
Keyword(s):  
Escherichia Coli ◽  
Bacterial Contamination ◽  
Faecal Contamination ◽  
E Coli ◽  
Production And Distribution ◽  
Microbiological Criteria ◽  
Minced Meat

Meat preparations are produced from one or more types of minced meat with added seasonings or additives, and are usually placed on the market under labels: ćevapčići, meat patty or hamburger/burger. The bacterial contamination of such products with Escherichia coli during production and distribution is invariably possible. In this paper, we have tested 50 samples of meat preparations for bacteria E. coli, whose presence points to the faecal contamination of food. Only 8 % of meat preparation samples had an E. coli count of less than 500 cfu/g, suggesting that all samples tested in this study yielded satisfactory results regarding the prescribed microbiological criteria for food.

The spectrum of Escherichia coli – Bacteroides fragilis pathogenic synergy in an intraabdominal infection model

1988 ◽  
Vol 34 (3) ◽  
pp. 352-357 ◽  
Author(s):  
O. D. Rotstein ◽  
J. Kao
Keyword(s):  
Escherichia Coli ◽  
Bacteroides Fragilis ◽  
Fibrin Clot ◽  
Experimental Models ◽  
Abscess Formation ◽  
Intraabdominal Infection ◽  
Infection Model ◽  
E Coli ◽  
Mixed Inocula ◽  
Number Of Bacteria

Pathogenic synergy between Escherichia coli and Bacteroides fragilis was investigated in an intraabdominal infection model. Defined inocula of E. coli and B. fragilis, alone or in combination, were enmeshed within a fibrin clot and surgically implanted into the peritoneal cavity of rats. A spectrum of bacterial synergy ranging from synergistic abscess formation to synergistic lethality was demonstrated using this model. The type of synergy exhibited was dependent upon the initial E. coli inoculum. When combined with B. fragilis, high inocula of E. coli (> 108 cfu/clot) produced synergistic lethality while low inocula (2 × 102 to 2 × 107 cfu/clot) resulted in synergistic abscess formation. With respect to abscess formation, there was reciprocal synergy between E. coli and B. fragilis. Abscesses resulting from mixed inocula were larger and had significantly higher numbers of E. coli and B. fragilis than abscesses initiated by monomicrobial inocula. These studies define a clinically relevant model of bacterial interactions in the setting of intraabdominal infection and suggest that conclusions drawn from experimental models of bacterial synergy should consider the type of model examined, the strains of bacteria studied, and the number of bacteria inoculated.

scholarly journals Bacterial Contamination at Whiteleg Shrimp (Litopeanaeus vannamei) in Aquaculture

2021 ◽  
Vol 6 (1) ◽  
pp. 136-145
Author(s):  
Mashuri Masri ◽  
Eka Sukmawaty ◽  
Fatmawati Nur ◽  
Suriani Suriani
Keyword(s):  
Escherichia Coli ◽  
Bacterial Contamination ◽  
Pond Water ◽  
Vibrio Cholera ◽  
E Coli ◽  
Safety Requirements ◽  
Negative Results ◽  
South Sulawesi ◽  
Fishery Products ◽  
Whiteleg Shrimp

Indonesia has a very high biodiversity, which has later become one of the natural products of interest to the international community, including fishery products. One of the high-demand Indonesian fishery products is whiteleg shrimp Litopeaneus vannamei. However, safety food Exported whiteleg shrimp products must meet the criteria, including free from bacterial contamination such as Escherichia coli, Salmonella, Vibrio cholera. This study attemptted to analyze E. coli, Salmonella, V. cholerae contamination in 3 ponds in Bojo, Cilellang, and Palanro Village in District Malusetasi, Barru Regency, South Sulawesi. Two samplings for each pond were conducted in the morning were pond water and  fresh whiteleg shrimp. SNI 2728-2018 specifies the quality and safety requirements for fresh shrimp. This standard applies to whole or headless fresh shrimp handled from fresh shrimp and does not apply to fresh shrimp that has undergone further processing. Based on SNI 2728-2018, the E. coli test showed positive in Cilellang Village (sample A) with 11 MPN/g, negative in Palanro Village (sample B) and in Bojo village (sample C) with the value of <2 MPN/g. Escherichia coli test showed positive in sample D (Vannamei shrimp in Cilellang Village) and sample E (Vannamei shrimp in Palanro Village) with 2.0 MPN/g, 17 MPN/g, respectively. Only sample F (Vannamei shrimp in Bojo village ) showed a negative result. As for the Salmonella test, positive results showed in sample A, while sample B and sample C showed negative results. The Vibrio cholerae test showed negative at all samples. . This study concludes that Whiteleg shrimp from ponds in Mallusetasi District is classified as safe for consumption.

scholarly journals Nanotextile membranes for bacteria Escherichia coli capturing

2010 ◽  
Vol 58 (5) ◽  
pp. 239-246
Author(s):  
Jaroslav Lev ◽  
Libor Kalhotka ◽  
Michal Černý
Keyword(s):  
Escherichia Coli ◽  
Experimental Study ◽  
Microbial Contamination ◽  
Model Simulation ◽  
Water Filtration ◽  
Contaminated Water ◽  
Air Filtration ◽  
E Coli ◽  
Before And After ◽  
Input Side

The article describes an experimental study dealing with the possibility of nanotextile materials usa­ge for microbiologically contaminated water filtration. The aim of the study is to verify filtration ability of different nanotextile materials and evaluate the possibilities of practical usage. Good detention ability of these materials in the air filtration is the presumption for nanotextile to be used for bacteria filtration from a liquid. High nanotextile porosity with the nanotextile pores dimensions smaller than a bacteria size predicates the possibility of a successful usage of these materials. For the experiment were used materials made from electrospinning nanofibres under the label PA612, PUR1, PUR2 s PUR3 on the supporting unwoven textiles (viscose and PP). As a model simulation of the microbial contamination, bacteria Escherichia coli was chosen. Contaminated water was filtered during the overpressure activity of 105Pa on the input side of the filter from the mentioned material. After three-day incubation on the nutrient medium, cultures found in the samples before and after filtration were compared. In the filtrated water, bacteria E. coli were indicated, which did not verify the theoretical presumptions about an absolut bacteria detention. However, used materials caught at least 94% of bacteria in case of material PUR1 and up to 99,996% in case of material PUR2. These results predict the possibility of producing effective nanotextile filters for microbiologically contaminated water filtration.Recommendation: For the production of materials with better filtrating qualities, experiments need to be done, enabling better understanding of the bacteria detention mechanisms on the nanotextile material, and parameters of the used materials that influence the filtrating abilities need to be verified.

scholarly journals Bacterial Contamination of Healthcare worker’s Mobile Phones; a Case Study at Two Referral Hospitals in Uganda

2021 ◽  
Author(s):  
Tusabe Fred
Keyword(s):  
Mobile Phone ◽  
Mobile Phones ◽  
Healthcare Workers ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
E Coli ◽  
Referral Hospitals

Abstract Background Hospital and Community-acquired infections are escalating and pose significant public health unhealthiness worldwide. The advancements of telemedicine and automation of healthcare records are supported by cellphones, laptops and wearable devices. This study focused on the incidence of healthcare workers’ mobile phones becoming contaminated with pathogenic bacteria and their possible roles as vehicles of transmission of antimicrobial-resistant bacteria.Method: A case study at two referral hospitals in Uganda between May and October 2020. Self-administered questionnaires were administered to participants after informed consent. Mobile phones of the participants in different departments of the hospitals were swabbed and samples were collected and transported to the microbiology laboratory for bacterial culture and antimicrobial susceptibility tests. Results: The point prevalence of Healthcare workers’ mobile phone bacterial contamination with one or more species was 93%. Organisms isolated were E. coli 5.6% (1), Micrococcus spp 11.1% (2), Coagulase-negative staphylococci, CoNS, 61.1% (11) and Bacillus spp 22.2% (4). About 45% of the organisms were multidrug-resistant. Resistance was major to penicillin, cotrimoxazole, ciprofloxacin and Gentamycin respectively. The isolated E. coli was resistant to all antibiotics used in the study. Only 15% (2) of the participants disinfected their phones at least once a week and 8% cleaned their hands after using a mobile phone.Conclusion: Healthcare Workers’ mobile phones can act as fomites for the transmission of multidrug-resistant micro-organisms. This study provides strong evidence for developing and strengthening disinfection protocols for mobile phones and does not underscore the importance of hand hygiene in the middle of a patient encounter especially when the HCW grabs a phone but doesn't re-clean their hands before patient contact.

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