scholarly journals Study the anti – inflammatory effect of Stevia rebaudiana sweetness in vitro

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Meison Abdulbary ◽  
Albasheer Ammar ◽  
Muhanad Mohammed ◽  
Wafaa Yousif ◽  
Suaad Lateef Ibrahim
Keyword(s):  
Aqueous Extract ◽  
Protein Denaturation ◽  
Daily Intake ◽  
Stevia Rebaudiana ◽  
Industrial Sector ◽  
Negative Control ◽  
Chemical Examination ◽  
Anti Inflammation ◽  
Inflammatory Effect

Because of the high rates of diabetes, overweight disorders and fatness, the need and interest in finding alternatives to sugar has increased, especially in the industrial sector in parallel with reducing the daily intake of sugar by human. The idea of this research was developed to identify the active ingredients which include in the  plant aqueous extract of Stevia rebaudiana. Chemical examination showed the glycosides and phenolic compounds presence of in the Stevia rebaudiana plant. The effect of Stevia rebaudiana leaves products aqueous extract as anti-inflammation  measured in vitro with two procedures , The repression of protein denaturation and hemolysis stimulated by heating and results showed Stevia rebaudiana efficiency with compared to typical medicine (Aspirin) and negative control groups.

In vitro antihemolytic effect, in vivo anti inflammatory and In vitro antioxidant activity of Anchusa azurea Mill

2021 ◽  
Vol 20 ◽  
Author(s):  
Boussoualim Naouel ◽  
Trabsa Hayat ◽  
Krache Imane ◽  
Ouhida Soraya ◽  
Arrar Lekhmissi ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Folk Medicine ◽  
Oxidative Degradation ◽  
Negative Control ◽  
Dependent Manner ◽  
Anti Inflammatory ◽  
Β Carotene ◽  
Inflammatory Effect

Background: Anchusa azurea Mill. (AA) is a medicinal plant largely used traditionally in folk medicine in Algeria, it is locally named: hamham. It is effective in the treatment of various diseases. Objectives: The aim of the present study is to determine the antioxidant, anti-inflammatory and anti-hemolytic effects of phenolic fractions from Anchusa azurea Mill. Methods: In this study, various extracts from Anchusa azurea Mill. (AA) using solvents with increasing polarity were prepared. The quantification of polyphenols and flavonoids was determined. The anti-radical activity of the different extracts was evaluated using DPPH and by measuring the inhibition of the oxidative degradation of β-carotene. The In vitro antihemolytic effect of the plant extracts is determined (CrE, ChE, AcE and AqE). For each extract, four concentrations were tested: 10.59, 21.18, 42.37, 84.74 µg/ml. Vitamin C is used as a standard. Free-radical attack was measured by measuring the HT50 (Half-Hemolysis Time). The anti-inflammatory effect using PMA on mice of the methanolic extract (CrE) was evaluated. Results: The quantification of polyphenols and flavonoids showed that ethyl acetate extract (AcE) contains a higher amount of polyphenols. However, chloroform extract (ChE) presents a higher amount of flavonoids. AcE showed an important scavenging activity using the DPPH radical (IC50= 68.35 µg/ml). The results showed that AcE also exhibited very great inhibition on the oxidation of β-carotene/linoleic acid (84.33%). All extracts increased the HT50 values (Half-Hemolysis Time) in a dose-dependent manner. The three highest concentrations (21.18, 42.37 and 84.74 µg / ml) of ChE caused a very significant delay (p ≤ 0.001) of hemolysis compared to the negative control and the positive control "VIT C". The anti-inflammatory effect using PMA on mice showed that the methanolic extract (CrE) of AA reduced the weight of the ear edema. Conclusions: This plant has a strong pharmacological power, which supports its traditional medicinal use.

scholarly journals In vitro antioxidant, anti-inflammatory and anti-cancer activities of methanolic extract of Asparagus horridus grows in North Cyprus Kuzey Kıbrıs da yetişen Asparagus horridus metanolik ekstraktının in-vitro antioksidan, anti-enflamatuar ve anti-kanser aktivitesi

2020 ◽  
Vol 45 (4) ◽  
pp. 365-372
Author(s):  
Ergul Mutlu Altundag ◽  
Duygu Gençalp ◽  
Cahit Özbilenler ◽  
Kübra Toprak ◽  
Namık Kerküklü
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Protein Denaturation ◽  
Soxhlet Extraction ◽  
Edible Plant ◽  
North Cyprus ◽  
Anti Cancer ◽  
Anti Inflammatory ◽  
Inflammatory Effect

AbstractBackgroundAsparagus horridus is an edible plant known as “Ayrelli” in North Cyprus. The scientific literature has not yet submitted a report about the antioxidant, anti-inflammatory and anti-cancer activities of A. horridus plant from North Cyprus until now. The purpose of the research was to determine the antioxidant, anti-inflammatory and anti-cancer activities of A. horridus.Materials and methodsSoxhlet extraction of A. horridus was performed using methanol. Antioxidant activity was determined by DPPH, TFC, FRAP and TPC assays. Protein-denaturation assay was performed to determine the anti-inflammatory effect. The anti-cancer effects of the extract on HepG2 and B-CPAP cell lines were determined with MTT assay.ResultsAntioxidant activity for A. horridus extract was determined by DPPH (50%), TFC (266.26 μg QUE/mg extract), FRAP (1.27 μg FeSO4/mg extract) and TPC (167.613 μg GAE/mg extract) assays at 25 mg/mL. Inhibition of protein-denaturation activity was found as 29.42% at 25 mg/mL. After 24 h of the extract treatment, cell proliferation of HepG2 and B-CPAP cancer cells were inhibited at IC50 values 63.24 μg/mL and 101.24 μg/mL, respectively.ConclusionThese results have shown that the methanol extract of A. horridus grows in North Cyprus has antioxidant, anti-inflammatory and anti-cancer activities.

scholarly journals Insights into the Invitro Antioxidant, Anti-Inflammatory and Anticancer Activities of Limonia acidissima Fruits

2021 ◽  
Vol 11 (3) ◽  
Author(s):  
S. Sujitha
Keyword(s):  
Ascorbic Acid ◽  
Aqueous Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Apple Fruit ◽  
Fruit Pulp ◽  
Anticancer Activities ◽  
Anti Inflammatory ◽  
Limonia Acidissima

Wood apple botanically identified as Limonia acidissima is an indigenous fruit with amazing nutritional and health benefits. Reports from traditional literature of Ayurveda and Siddha portrays the medicinal properties of this fruit. The scooped pulp of the ripe fruit is consumed as such or it can be made into different recipes. But inclusion of this fruit in our diet is not found as a regular practice. Many people are still unaware of the benefits of this fruit. Hence, this study was taken up to unravel the biological potencies of this fruit by conducting in vitro experiments. Phytochemicals such as alkaloids, flavonoids, phenols, saponins and ascorbic acid have been estimated. Anti-inflammatory activity of the aqueous extract of fruit pulp combined with outer rind has been evaluated through inhibition of albumin denaturation. Among the 5 different concentrations (200, 400, 600, 800, 1000 µg/ml), at 1000 µg/ml wood apple has shown 74.55% of protein denaturation inhibition which was compared with standard Diclofenac sodium. Antioxidant capacity of the extract was expressed as mg/100g ascorbic acid equivalent through phosphomolybdenum assay. Dose dependent increase in the antioxidant activity was observed. About 8 different concentrations of the aqueous extract of L. acidissima were evaluated for their cytotoxic activity on MCF 7 cell line. At a concentration of 1000 µg/ml, the extract has shown 93.43% of cytotoxicity and 6.57% of cell viability. Apoptotic induction was evaluated and confirmed by the formation of DNA ladders through DNA fragmentation assay. GCMS analysis of wood apple fruit pulp and rind revealed the presence of several phytochemicals among which many of them had therapeutic activity reported earlier.

Pharmacological and Biochemical evaluation of anti-arthritic activity of Justicia gendarussa extract in FCA induced arthritis in Wistar rats

2021 ◽  
Vol 12 (3) ◽  
pp. 1699-1708
Author(s):  
Ashish ◽  
Anjali ◽  
Praveen K Dixit ◽  
Nagarajan K
Keyword(s):  
Body Weight ◽  
Protein Denaturation ◽  
Hematological Parameters ◽  
Diclofenac Sodium ◽  
Paw Edema ◽  
Arthritis Model ◽  
Inflammatory Models ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The present study is carried out to evaluate anti-inflammatory and anti-arthritic potential of Justicia gendarussa. Leaves and stem extracted with ethanol and chloroform. These extract is tested against in-vitro (HRBC membrane stabilization method and Inhibition of protein denaturation method) and in-vivo (Carrageenan induced paw edema model) anti-inflammatory models. To assess the anti-arthritic activity, FCA induced arthritis model is used. Assessment of arthritis is done by paw volume, joint diameter, body weight, biochemical parameters, hematological parameters (Hb, RBC, WBC, ESR). The effect of in-vitro anti-inflammatory model depends on concentration. Both test extract and standard Diclofenac sodium has been shown concentration dependent effect. The maximum anti-inflammatory effect of the test extract achieved at 2000μg/ml. The test (JGLE, JGLC, JGSE) has been shown inhibition of paw edema induced by carrageenan at 50mg/kg body weight. The extract JGLE, JGLC, JGSE at 50mg/kg body weight and 100mg/kg body weight orally showed the significant (P< 0.05) and dose dependent inhibitory effect against FCA induced arthritis model. Diclofenac sodium 20mg/kg body weight orally is used as a standard. JGLE exhibit more significant and most promising anti-arthritic and anti-inflammatory effect than other extracts these effects support the traditional role of J. gendarussa in arthritis and other inflammatory condition.

scholarly journals Factors Regulating Nitric Oxide Production in Piper Sarmentosum Aqueous Extract Treated Spontaneous Hypertensive Rats

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Maizura Mohd Zainudin ◽  
Taher F T Elshami ◽  
Hidayatul Radziah Ismawi ◽  
Fatimatuzzhara Hashim Fauzy
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Blood Pressure Monitoring ◽  
Negative Control ◽  
Total Phenolic ◽  
No Production ◽  
Invasive Blood Pressure Monitoring

Introduction: High blood pressure is a major risk factor for cardiovascular diseases which is one of the leading causes of death worldwide. Piper sarmentosum (PS) leaves have been widely used in traditional medicine and it has been proven for its antihypertensive and antioxidant effects. This study aims to evaluate the antihypertensive potential of PS aqueous extract (PSAE) and to investigate the factors modulating nitric oxide (NO) production through its anti-oxidant activities. Materials and Methods: PS leaves were extracted with distilled water and freezedried. The PSAE was examined to quantify their antioxidant activities through DPPH and FRAP test. It is also screened for total phenolic and flavonoids content. The antihypertensive effect of PSAE in SHR was evaluated using four different groups (n=6); C group (negative control), K group (PSAE 500mg/kg), P group (3 mg/kg perindopril) and M group (PSAE + 1.5 mg/kg perindopril). PSAE and other treatment were given via oral gavage for 28 consecutive days. The blood pressure and heart rate were determined using the non-invasive blood pressure monitoring (NIBPM) tail cuff technique and recorded weekly for four weeks. SHR’s blood was collected for the determination of serum NO level using Griess assay. Asymmetric dimethylarginine (ADMA) and arginine levels were determined using HPLC. Results: PSAE shows good in-vitro antioxidant activities with significant reduction of BP and decreased serum ADMA level. Conclusion: PSAE enhances the clearance of ADMA that maintains arginine level, thus increasing the serum NO level, which ameliorate the blood pressure of SHR.

scholarly journals In vitro Anti-Arthritic activity of Pseudarthria viscida against Protein Denaturation and Proteinase enzyme

2020 ◽  
Vol 11 (2) ◽  
pp. 284-288
Author(s):  
Pandian P
Keyword(s):  
Enzyme Activity ◽  
Aqueous Extract ◽  
Protein Denaturation ◽  
Bone Erosion ◽  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Maximum Inhibition

Arthritis is an autoimmune with chronic inflammatory, the patient has very painful due deformities and bone erosion which is caused by damage of the joints. The plant Pseudarthria viscida was collected from the Thirunelveli district and extracted with aqueous and ethanol solvent. The two method was used for determination of invitro anti-arthritic activity. The Inhibition of Protein Denaturation Method shows the anti-arthritic activity with the value from 40.46±0.72 to 78.36±0.64 for aqueous extract and 48.62±0.86 to 84.42±0.86 for ethanol extract and Inhibition of Proteinase Enzyme Activity shows 38.62±0.32 to 72.58±0.58 in aqueous extract and 46.28±0.58 to 80.52±0.56 in ethanol extract. Diclofenac sodium were used as standard, the concentration is 100, 200, 300, 400, and 500. In both the method the concentration of 500Microgram per milliliters shows maximum inhibition and compare to both extract the ethanol shows better activity than aqueous extract.

scholarly journals In-vitro Anti-inflammatory activity of Liquorice (Glycyrrhiza glabra) using Aqueous Extract

2020 ◽  
Vol 11 (1) ◽  
pp. 657-662
Author(s):  
Vasanth M P ◽  
Purushotham KG ◽  
Sathish M ◽  
Vimal Raj D ◽  
Venkatesh M
Keyword(s):  
Medicinal Plant ◽  
Cell Line ◽  
Aqueous Extract ◽  
Cytotoxicity Assay ◽  
Glycyrrhiza Glabra ◽  
Peroxide Radical ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Anti Inflammation

The G.glabra is otherwise called liquorice is a medicinal plant is used for various diseases like cold, cough, hypokalemia and muscle weakness, etc. The liquorice family belongs to the Fabaceae family of the G.glabra. Hence this study tells about the anti-inflammatory and antioxidants.  The quantitative study of phytochemical analysis, antioxidant and Anti-inflammatory, cytotoxicity assay using a response from the root extract of G.glabra  The results are showed above preliminary activity phytochemicals were present Alkaloids, Flavonoids, Coumarin, Saponins, Terpenoids, Steroids, Cardiac Glycosides. The antioxidant activity of aqueous extract of G. glabra were evaluated with the 2,2-diphenyl-1-picryl hydrazyl (DPPH), nitric oxide radical (NO), superoxide radical (SO), hydrogen peroxide radical (H2O2), and hydroxyl radical (HO) scavenging activity. This study shows about the activity of Glycyrrhiza glabra herbal medicinal plant aqueous extract using antioxidant assays, Anti-inflammatory assays.  The in vitro MTT 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Cytotoxicity assay were studied GG(G.glabra)  extract using the macrophages  Raw 246.7 Cell line. The cell line assay were studied in two different activity. One is anti-inflammation studies, and another is cytotoxicty of GG aqueous extract. The anti-inflammation IC50 value is 143.65, and GG extract dry sample were against the IC50 value is 326.27. From above, the results were a potential activity of GG extract R-value 0f 0.991.

scholarly journals The anti-arthritic and anti-inflammatory activities of aqueous extract powder bark of Anthocleista schweinfurthii Gilg (Loganiaceae)

2018 ◽  
Vol 8 (6-s) ◽  
pp. 174-181
Author(s):  
Valdi Steve Djova ◽  
Ascension Maximilienne Nyegue ◽  
François-Xavier Etoa
Keyword(s):  
Aqueous Extract ◽  
Selective Inhibition ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Inflammatory Effect

  Aqueous extract bark of Anthocleista schweinfurthii (ASE) was investigated for anti-arthritic, anti-inflammatory activities and phytochemicals screening. Phytochemical screening was carried out according, to LC-MS procedures, the anti-inflammatory effect of ASE was evaluated in vitro inhibition of 15-lipoxygenase (15-LOX) enzyme and  proteinase inhibiting action assays. The anti-arthritic activity was performed according to the Bovine Serum Albumin (BSA) test. The chemical composition of ASE showed that it was mainly composed of isocyanate, biphenyltriol, methoxyflavanone, soscoparin and glucopyroside. In the anti-inflammatory assays, ASE showed selective inhibition of 15-lipooxygenase with IC50 value of and moderate activity against the inhibition of proteinase action with IC50 value of L. Both the methods  showed that the ASE posses good anti-inflammatory activity. ASE had strong in vitro anti-arthritic activity with IC50 of , the positive control Diclofenac had IC50 value of  The result of this study justified the use of this plant in traditional Cameroonian medicine in the treatment of inflammation. ASE may offer a new source of potential therapeutic agents for the effective treatment of arthritis and inflammation.    Keywords: Anthocleista schweinfurthii, phytochemical screening, anti-arthritic activity, anti-inflammatory activity,     

scholarly journals Anti-inflammatory effect of aqueous extract from Kawachi-bankan (Citrus maxima) peel in vitro and in vivo

2019 ◽  
Vol 71 (4) ◽  
pp. 797-807
Author(s):  
Momoko Ishida ◽  
Chihiro Takekuni ◽  
Kosuke Nishi ◽  
Takuya Sugahara
Keyword(s):  
Aqueous Extract ◽  
Citrus Maxima ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Anti-Inflammatory Effect of Allium ursinum

2014 ◽  
Vol 6 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Alina Elena PÂRVU ◽  
Florinela CĂTOI ◽  
Sameera DEELAWAR ◽  
Darshana SARUP ◽  
Marcel PÂRVU
Keyword(s):  
Oxidative Stress ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Stress Index ◽  
Allium Ursinum ◽  
Total Oxidative Status ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The aim of the present study was to evaluate Allium ursinum leaves and flowers extract anti-inflammatory effect. Plant extract 1:1 (w:v) was prepared from A. ursinum leaves by a modified Squibb repercolation method. The in vivo anti-inflammatory effects were evaluated on a rat turpentine oil-induced inflammation (i.m. 6 mL/kg BW). The animals were randomly assigned to nine groups (n=8): negative control, inflammation, A. ursinum flower extract (AUF), A. ursinum leaves extract (AUL), indomethacin (INDO) (20 mg/kg BW), aminoguanidine (AG) (50 mg/kg b.w./d i.p.) as a selective NOS2 inhibitor, NG-nitro L-arginine methyl ester (NAME) (5 mg/kg b.w./d i.p.) as a nonselective NOS inhibitor, L-arginine (ARG) (100 mg/kg b.w./d i.p.), NO synthesis substrate, and Trolox (20 mg/kg b.w./d i.p) as an antioxidant. At 24h from inflammation induction total oxidative status (TOS), oxidative stress index (OSI), nitric oxide (NOx) and in vitro phagocytosis test were reduced and the total antioxidative reactivity (TAR) was increased by the testes plant extracts. AUF had a better inhibitory effect than AUL. In conclusion, we provided evidence for the hypothesis that A. ursinum leaves and flowers extract exerts anti-inflammatory activity by inhibiting the phagocytosis through the reduction of the nitro-oxidative stress.

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