scholarly journals A Milestone In Botanical Mosquito Repellents: Novel PMD-Based Formulation Protects More Than Twice As Long As High-Concentration Deet and Other Leading Products

2019 ◽  
Vol 35 (3) ◽  
pp. 186-191 ◽  
Author(s):  
Scott P. Carroll ◽  
Jeffrey Venturino ◽  
John H. Davies
Keyword(s):  
High Performance ◽  
Personal Protection ◽  
Prolonged Action ◽  
High Concentration ◽  
Current Standard ◽  
Protection Time ◽  
Practical Strategy ◽  
Control And Prevention ◽  
The Usa ◽  
Mosquito Repellents

ABSTRACT The use of skin-applied repellents is the primary method recommended by the Centers for Disease Control and Prevention for personal protection against biting mosquitoes. Historically, the majority of long-efficacy mosquito repellents have been N,N diethyl-3-methylbenzamide (deet)–based. Recently, a variety of new botanical formulations have been marketed, but their protection times generally continue to fall well short of high-concentration deet products. We present a laboratory arm-in-cage study of a Neo-Innova® repellent that has a prolonged action “NEO-PART®” (Prolonged Action Release Technology) formulation with 40% Citriodiol®. This formulation provides the botanical molecule para-menthane 3,8-diol (PMD) at 25% w/v of the total formulation. Against Aedes aegypti, Neo-Innova's mean complete protection time (CPT; 14.2 h) was approximately 2 to 3 times longer than that of 5 leading high-performance repellents marketed in the USA, including 25% deet and a 20% PMD ethanolic formulation. When testing Neo-Innova, 5 of the 6 subjects had no landings after 15 h. The 6th had single landings at 10 and 11 h (individual CPT of 10 h), but received no additional landings in further exposures made at 13 and 15 h. Neo-Innova repellency against Culex quinquefasciatus was similarly prolonged. The tremendous increment in repellency duration observed for the Neo-Innova product, when compared with both current standard and botanical repellent options, represents a milestone in repellent development and supports “once-a-day” applications as a practical strategy for personal protection against mosquitoes.

Quantitation of Δ8-THC, Δ9-THC, Cannabidiol and 10 Other Cannabinoids and Metabolites in Oral Fluid by HPLC–MS-MS

2020 ◽  
Author(s):  
Lin Lin ◽  
Piyadarsha Amaratunga ◽  
Jerome Reed ◽  
Pornkamol Huang ◽  
Bridget Lorenz Lemberg ◽  
...  
Keyword(s):  
Human Subject ◽  
High Performance ◽  
Oral Fluid ◽  
Solid Phase ◽  
Forensic Toxicology ◽  
High Concentration ◽  
Driving Impairment ◽  
Three Samples ◽  
Cannabidiolic Acid ◽  
Liquid Chromatography Tandem Mass

Abstract Quantitative analysis of Δ9-tetrahydrocannabinol (Δ9-THC) in oral fluid has gained increasing interest in clinical and forensic toxicology laboratories. New medicinal and/or recreational cannabinoid products require laboratories to distinguish different patterns of cannabinoid use. This study validated a high-performance liquid chromatography-tandem mass spectrometry method for 13 different cannabinoids, including (-)-trans-Δ8-tetrahydrocannabinol (Δ8-THC), (-)-trans-Δ9-tetrahydrocannabinol (Δ9-THC), cannabidiol (CBD), Δ9-tetrahydrocannabinolic acid-A (Δ9-THCA-A), cannabidiolic acid (CBDA), 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-Δ9-THC), 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (Δ9-THCCOOH), tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabidiorcol (CBD-C1), cannabichromene (CBC), cannabinol (CBN) and cannabigerol (CBG), in oral fluid. Baseline separation was achieved in the entire quantitation range between Δ9-THC and its isomer Δ8-THC. The quantitation range of Δ9-THC, Δ8-THC and CBD was from 0.1 to 800 ng/mL. Two hundred human subject oral fluid samples were analyzed with this method after solid phase extraction. Among the 200 human subject oral fluid samples, all 13 cannabinoid analytes were confirmed in at least one sample. Δ8-THC was confirmed in 11 samples, with or without the presence of Δ9-THC. A high concentration of 11-OH-Δ9-THC or Δ9-THCCOOH (>400 ng/mL) was confirmed in three samples. CBD, Δ9-THCA-A, THCV, CBN and CBG were confirmed in 74, 39, 44, 107 and 112 of the 179 confirmed Δ9-THC-positive samples, respectively. The quantitation of multiple cannabinoids and metabolites in oral fluid simultaneously provides valuable information for revealing cannabinoid consumption and interpreting cannabinoid-induced driving impairment.

scholarly journals Concentration of Bioactive Phenolic Compounds in Olive Mill Wastewater by Direct Contact Membrane Distillation

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1808
Author(s):  
Rosa Tundis ◽  
Carmela Conidi ◽  
Monica R. Loizzo ◽  
Vincenzo Sicari ◽  
Rosa Romeo ◽  
...  
Keyword(s):  
Direct Contact ◽  
High Performance ◽  
Biological Properties ◽  
Membrane Distillation ◽  
Olive Mill Wastewater ◽  
High Concentration ◽  
Direct Contact Membrane Distillation ◽  
Reducing Activity ◽  
Β Carotene ◽  
Olive Mill

Olive mill wastewater (OMW), generated as a by-product of olive oil production, is considered one of the most polluting effluents produced by the agro-food industry, due to its high concentration of organic matter and nutrients. However, OMW is rich in several polyphenols, representing compounds with remarkable biological properties. This study aimed to analyze the chemical profile as well as the antioxidant and anti-obesity properties of concentrated fractions obtained from microfiltered OMW treated by direct contact membrane distillation (DCMD). Ultra-high performance liquid chromatography (UHPLC) analyses were applied to quantify some phenols selected as phytochemical markers. Moreover, α-Amylase, α-glucosidase, and lipase inhibitory activity were investigated together with the antioxidant activity by means of assays, namely β-carotene bleaching, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) diammonium salts, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and Ferric Reducing Activity Power (FRAP) tests. MD retentate—which has content of about five times greater of hydroxytyrosol and verbascoside and about 7 times greater of oleuropein than the feed—was more active as an antioxidant in all applied assays. Of interest is the result obtained in the DPPH test (an inhibitory concentration 50% (IC50) of 9.8 μg/mL in comparison to the feed (IC50 of 97.2 μg/mL)) and in the ABTS assay (an IC50 of 0.4 μg/mL in comparison to the feed (IC50 of 1.2 μg/mL)).

scholarly journals Elucidation of the Crystal Growth Characteristics of SnO2 Nanoaggregates Formed by Sequential Low-Temperature Sol-Gel Reaction and Freeze Drying

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1738
Author(s):  
Saeid Vafaei ◽  
Alexander Wolosz ◽  
Catlin Ethridge ◽  
Udo Schnupf ◽  
Nagisa Hattori ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Low Temperature ◽  
Photoelectron Spectroscopy ◽  
High Performance ◽  
Freeze Drying ◽  
Sno2 Nanoparticles ◽  
Sol Gel ◽  
Functional Materials ◽  
Cost Effective ◽  
High Concentration

SnO2 nanoparticles are regarded as attractive, functional materials because of their versatile applications. SnO2 nanoaggregates with single-nanometer-scale lumpy surfaces provide opportunities to enhance hetero-material interfacial areas, leading to the performance improvement of materials and devices. For the first time, we demonstrate that SnO2 nanoaggregates with oxygen vacancies can be produced by a simple, low-temperature sol-gel approach combined with freeze-drying. We characterize the initiation of the low-temperature crystal growth of the obtained SnO2 nanoaggregates using high-resolution transmission electron microscopy (HRTEM). The results indicate that Sn (II) hydroxide precursors are converted into submicrometer-scale nanoaggregates consisting of uniform SnO2 spherical nanocrystals (2~5 nm in size). As the sol-gel reaction time increases, further crystallization is observed through the neighboring particles in a confined part of the aggregates, while the specific surface areas of the SnO2 samples increase concomitantly. In addition, X-ray photoelectron spectroscopy (XPS) measurements suggest that Sn (II) ions exist in the SnO2 samples when the reactions are stopped after a short time or when a relatively high concentration of Sn (II) is involved in the corresponding sol-gel reactions. Understanding this low-temperature growth of 3D SnO2 will provide new avenues for developing and producing high-performance, photofunctional nanomaterials via a cost-effective and scalable method.

"HOW THE BRITISH VIEW US"

PEDIATRICS ◽  
1994 ◽  
Vol 94 (5) ◽  
pp. 731-731
Author(s):  
J. F. L.
Keyword(s):  
Good Reason ◽  
British Society ◽  
Personal Protection ◽  
Murder Rate ◽  
Three Generations ◽  
Local Police ◽  
The Usa

"American citizens are not protected by a law akin to the British firearms Act 1920. These regulations, along with the laws passed in 1936, 1968, and 1988, have protected British citizens for nearly three generations. Quite apart from America, "where buying a handgun is as easy as buying a tooth brush," British society is far more circumspect about private weaponry. To obtain a gun license, potential owners must satisfy local police that they have "good reason" for possessing a gun and that they are "fit to be entrusted with it." One of the guiding philosophies behind British gun restrictions—that personal protection is not a good reason for having a firearm—may seem untenable (possibly quaint) to some Americans. Yet defenseless Britons remain comparatively safe in their homes with a murder rate one tenth of that in the USA.

The long-term development of Russian biotech sector

foresight ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. 491-500 ◽  
Author(s):  
Anna Grebenyuk ◽  
Nikolai Ravin
Keyword(s):  
High Performance ◽  
Large Scale ◽  
Technological Development ◽  
Science And Technology ◽  
Plant Biotechnology ◽  
High Tech ◽  
Content Type ◽  
Research Areas ◽  
Research Activities ◽  
The Usa

Purpose To define strategic directions for the Russia’s social, economic, scientific and technological development in 2011-2013, a large-scale foresight study including the deep analysis of prospects of biotechnology development there was undertaken (Russia 2030: Science and Technology Foresight). This paper aims to present results of this research. Design/methodology/approach The study was based on a combination of technology-push and market-pull approaches that aimed not only to identify most promising science and technology (S&T) areas but also to understand how they can be realized in practice. Representatives from federal authorities, science and business were involved in the project to create future visions of technological directions; analyze grand challenges, weak signals and wild cards; and set research and development (R&D) priorities. Findings According to results of the study, Russia has a potential for biotech sector development, although the level of R&D in the majority of areas is lagging behind that in the USA and leading EU countries. However, there are several advanced applied research areas where efforts can be focused. Among them are high-performance genomics and post-genomics research platforms, systems and structural biology, microbial metabolic engineering, plant biotechnology and microbial strains and consortia for development of symbiotic plant–microbial communities. Originality/value Concentration of available resources of government and business on biotechnological sector development can help to find answers for challenges that Russia faces today or will face tomorrow. It will help to pick up on the current level of research activities, improve the quality of personnel training, make this area the engine of the economy and carry out the so-called new industrialization of the country, building a new, high-tech device industry.

scholarly journals A sensitive and reliable RP-HPLC method for the detection of cimetidine – a H2 receptor antagonist in human plasma

2019 ◽  
Vol 8 (3) ◽  
pp. 671-674
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Internal Standard ◽  
Bioequivalence Study ◽  
High Concentration ◽  
H2 Receptor Antagonist ◽  
Chromatography Method ◽  
Thaw Cycle ◽  
Limit Of Quantitation ◽  
The Mean

Bioanalytical methods for bioequivalence studies require high sensibility and rapidity due to the large number of samples and the low plasma concentration of drugs. The present study aimed to develop and validate a high-performance liquid chromatography method to quantify cimetidine (CMT) in human plasma and to apply it in a bioequivalence study. Spiked plasma of 500 µl (l, m and h concentration) was used for the assay. The HPLC injection volume was 20μl of the reconstitute sample where, 2 ml of ethyl acetate used for extraction purposes. Cimetidine was prepared separately for low (80 ng/ml), medium (2000 ng/ml) and high (3600 ng/ml) concentrations and internal standard (ranitidine) concentration was 3000 ng/ml. Freeze thawing and long terms stability were conducted at -25º c. The individual calibration curve for spiked standards was linear with R2= 0.99. The inaccuracy values for QC samples were within 15% of the actual value and not more than 20% for the LOQ. The limit of quantitation (LOQ) was 40 ng/ml, which was also the lowest concentration of cimetidine that was quantitated with the variability of 5.9%. The within day precision and between day precision for LOQ were 10.8 and 5.9 respectively. The retention time for the analyte was 4.1-4.5 minutes during the within a day and between day results. The mean % inaccuracy values for low, medium and high concentration were 6.8, 5.6 and 7.8 respectively for within day and 2.4, 6.1 and 7.9 respectively for between days. The within day and between day % inaccuracy for LOQ concentration was 12.4 and 5.5 respectively. The mean recoveries for low, medium and high concentration of cimetidine were 80.2, 70.9 and 74.2. The overall mean recovery for cimetidine was 75.1%. The maximum inaccuracy for freeze thaw cycle and long term stability samples for low, medium and high was found with CV less than 15% for all concentrations, indicating that cimetidine is stable. The developed method was precise and accurate and was suitable to be applied for the bioequivalence study of cimetidine.

scholarly journals Natural Anophelin mosquito repellents and phytochemical analysis of ethanol and hexane leaf extracts from four plant species

2021 ◽  
Vol 4 ◽  
Author(s):  
Emad I. Y. Adam ◽  
Yousif O.H.M. Assad ◽  
Marwa M. E. Eltohami ◽  
Nabil H.H. Bashir ◽  
Samira H. Abdelrahman ◽  
...  
Keyword(s):  
Eucalyptus Camaldulensis ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Protection Time ◽  
Cymbopogon Flexuosus ◽  
Toxic Agents ◽  
Mosquito Repellents ◽  
Ancient Times ◽  
Ethanol Extracts ◽  
Control Application

Currently mosquitoes control is the cornerstone to minimize the rising number of mosquito borne diseases. There is an urgent need looking for alternatives to the current reliance on synthetic insecticides for the vectors control. Application of active toxic agents from plant extracts as an alternative control strategy was available from ancient times. These are non-toxic, easily available at affordable prices, biodegradable and show broad-spectrum target-specific activities against different species of vectors. Natural products with repellency properties are urgently needed. An insect repellent works by masking human scent, and a number of natural and synthetic mosquito repellents were studied. The main aim of this study was to identify the phytochemicals and compare their potentials as mosquito repellents from the ethanol (Et. OH) and hexane (hex) leaf extracts (LEs) of Ocimum basilicum, Coleus forskohlii, Eucalyptus camaldulensis and Cymbopogon flexuosus under laboratory conditions at 50 and 100% concentrations. Phytochemical analysis showed that alkaloids, saponins, flavonoids, terpenes, tannins and terpenoids were present/or absent in the 4 plant-LEs. At 50% concentration of Et. OH extracts, C. forskohlii exhibited higher repellency potential on Anopheles gambiae with protection time of 137.3 min, while O. basilicum, E. camaldulensis and Cy. flexuosus registered protection time of 30.6, 15.3 and 19 min, respectively. At 100% concentration of ethanol- extracts, C. forskohlii caused the highest protection time against the vector with protection time of 182 min. O. basilicum, E. camaldulensis and Cy. flexuosus were equally less potent against the insect, with protection time 42.6, 32.6 and 28 min, respectively. Regarding hex-extract, at 50% concentration of C. forskohlii, the highest repellency potential , with protection time of 174 was registered, while O. basilicum, E. camaldulensis and Cy. flexuosus registered protection times of 44, 18 and 28.6 min, respectively. However, the 100% concentration of Et. OH-extracts of, C. forskohlii and O. basilicum exhibited the highest protection time, with protection times of 228 and 116min, respectively. E. camaldulensis and Cy. flexuosus were less potent, with protection times 28.6 and 54.6 min, respectively. The EOs of C. forskohlii and O. basilicum L EOs proved to have potentials as repellency agents against Anopheles. GC-MS analysis of the extracts identified the compounds of both solvents extracts and the terpens were the major compounds. According to the results, further studies are required, especially on the extracts of basil and coleus since they proved to be effective.

Immunochemical characterization and distribution of calcitonin in the lizard

1981 ◽  
Vol 97 (3) ◽  
pp. 427-432 ◽  
Author(s):  
F. Galan Galan ◽  
R. M. Rogers ◽  
Samia I. Girgis ◽  
T. R. Arnett ◽  
Mariella Ravazzola ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Concentration ◽  
Parallel Displacement ◽  
Immunochemical Characterization ◽  
Common Wall ◽  
Brain Extracts ◽  
Ultimobranchial Gland

Abstract. Immunological and chromatographic methods were used to investigate the distribution of calcitonin (CT) in various tissues of Lacerta muralis, a common wall lizard. Salmon CT-like immunoreactivity was found in high concentration in extracts of ultimobranchial gland (UBG) and in significant amounts in lung and brain extracts, but not in other tissues (oesophagus, stomach, duodenum, liver, skin, muscle and thyroid). Serial dilution of UBG, lung and brain extracts gave parallel displacement curves to that of synthetic salmon CT (sCT), but no reactivity was found in the human CT assay. On Sephadex G-50, UBG, lung and brain extracts contained an sCT-like immunoreactive peak which co-eluted with synthetic sCT. However, on high performance liquid chromatography (HPLC) the sCT-like peak in UBG, lung and brain extracts eluted 2 ml later than synthetic sCT. This paper describes the first immunochemical characterization of CT in the UBG of the lizard, demonstrates the distribution of extra-ultimobranchial CT and throws some light on the evolution of the calcitonins.

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