scholarly journals PATHOLOGICAL STUDY ON LARYNGOTRACHEITIS IN LAYERS

2021 ◽  
Vol 22 (1) ◽  
pp. 50-66
Author(s):  
H.M.I. Al—Hyali ◽  
K.H.Z. Al—Job0ury ◽  
A.O. Ismail
Keyword(s):  
Cell Surface ◽  
Inclusion Bodies ◽  
Mononuclear Cells ◽  
Histopathological Examination ◽  
Mucosal Cell ◽  
Pathological Study ◽  
Presumptive Diagnosis ◽  
Facial Swelling ◽  
Infectious Laryngotracheitis ◽  
Clinical Conditions

A clinical conditions resembling infectious laryngotracheitis were diagnosed amongst 20,000 , 18,000 , 16,000 and 17,500 respectively, 28-30 weeks old, ISA brown layers. The hens had nasal discharges, moist rales, coughing and gasping. Hemorrhagic mucous was ejected during sneezing, lacrirnation, conjunctivitis with facial swelling with eyes partially or completely" closed. Postmortum examination of dead and affected hens revealed hemorrhagic tracheitis with thin pseudomembrane formation. The larynx, congested with petechia on mucous membrane, Infraorbital sinus contained clear thick fluid. Histopathological examination of trachea showed hypertrophy, of epithelial pseudostratification of the mucosal cell surface, extensive hemorrhages and desquamative necrotizing tracheitis with mononuclear cells infiltration. Multinucleated gaint cells in theciliated epithelium containing round, oval shaped intranuclear A inclusion bodies. The lamina propiia shows edema, marked‘ congestion with lymphocytic infiltration.A presumptive diagnosis of laryngotracheitis was made.

scholarly journals An Overview of Sino-nasal masses: A study from Dhaka Medical College Hospital

2020 ◽  
Vol 28 (1) ◽  
pp. 49-53
Author(s):  
Debesh Chandra Talukder ◽  
Satinath Chandra Sarker ◽  
Syed Ali Ahasan ◽  
Muhammad Mozammal Haque ◽  
Mostafa Kamal Arefin ◽  
...  
Keyword(s):  
Nasal Cavity ◽  
Histopathological Examination ◽  
Wide Spectrum ◽  
Lateral Wall ◽  
Sinus Surgery ◽  
Presumptive Diagnosis ◽  
College Hospital ◽  
Medical College ◽  
Major Mode ◽  
Facial Swelling

Background: To study the incidence, clinical presentation and to observe comparison of different sinonasal masses. Materials and Methods: This Prospective study was carried out from January 2017 to December 2018. Data were collected from 100 patients presented with sinonasal masses. This study is based on history, clinical, radiological, laboratory and histopathological examination. Results: During the study period, 100 patients presented with sinonasal masses (male 60, female 40; age group 8-70 years).Nasal polyploid masses were non-neoplastic in 70 (70%) subjects, and neoplastic in 30 (30%) cases. Nasal obstruction was the most common (95%) presenting complaint, followed by rhinorrhoea (48%), hyposmia (30%), intermittent epistaxis (17%), headache (15%), facial swelling (10%) and eye-related symptoms (10%). The most common site of origin of polyploid masses was the middle meatus (54%) followed by the lateral wall of the nasal cavity (15%) and superior meatus (11%). unilateral nasal masses was present in 49% patients, while the remaining patients had bilateral nasal masses. Allergic (60%) and inflammatory (28.5%) polyps were the most common non-neoplastic mass, inverted papilloma (41.17%) and haemangioma (35.29%) were most common benign neoplastic mass; 92.3% of all malignant masses were squamous cell carcinoma. Surgery was the major mode of treatment. it included Caldwell-luc operation (7%), polypectomy (10%), excision of mass (25.0%) and functional endoscopic sinus surgery (44%). Malignancies were treated with radiotherapy or Surgery followed by radiotherapy or Chemoradiotherapy. Conclusions: The masses in nasal cavity, paranasal sinuses, and nasopharynx encompass a wide spectrum of common and rare diseases and are very common lesions encountered in clinical practice. Endoscopic examination and advanced imaging technique help to reach a presumptive diagnosis but histopathological examination remains the mainstay of final definitive diagnosis. J Dhaka Medical College, Vol. 28, No.1, April, 2019, Page 49-53

Canine Parvovirus Infections in Taiwanese Pangolins (Manis pentadactyla pentadactyla)

2021 ◽  
pp. 030098582110021
Author(s):  
Yen Chi Chang ◽  
Zhi Yi Lin ◽  
Yan Xiu Lin ◽  
Kuei Hsien Lin ◽  
Fang Tse Chan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Internal Control ◽  
Mononuclear Cells ◽  
Histopathological Examination ◽  
Domestic Animals ◽  
Canine Parvovirus ◽  
Necrotic Enteritis ◽  
Intestinal Lesions ◽  
Manis Pentadactyla ◽  
Systemic Infections

Canine parvovirus type 2 (CPV-2) is among the most important and highly contagious pathogens that cause enteric or systemic infections in domestic and nondomestic carnivores. However, the spillover of CPV-2 to noncarnivores is rarely mentioned. Taiwanese pangolins ( Manis pentadactyla pentadactyla) are threatened due to habitat fragmentation and prevalent animal trafficking. Interactions between Taiwanese pangolins, humans, and domestic animals have become more frequent in recent years. However, information about the susceptibility of pangolins to common infectious agents of domestic animals has been lacking. From October 2017 to June 2019, 4 pangolins that were rescued and treated in wildlife rescue centers in central and northern Taiwan presented with gastrointestinal signs. Gross and histopathological examination revealed the main pathologic changes to be necrotic enteritis with involvement of the crypts in all intestinal segments in 2 pangolins. By immunohistochemistry for CPV-2, there was positive labeling of cryptal epithelium throughout the intestine, and immunolabeling was also present in epidermal cells adjacent to a surgical amputation site, and in mononuclear cells in lymphoid tissue. The other 2 pangolins had mild enteritis without crypt involvement, and no immunolabeling was detected. The nucleic acid sequences of polymerase chain reaction (PCR) amplicons from these 4 pangolins were identical to a Chinese CPV-2c strain from domestic dogs. Quantitative PCR revealed a higher ratio of CPV-2 nucleic acid to internal control gene in the 2 pangolins with severe intestinal lesions and positive immunoreactivity. Herein, we present evidence of CPV-2 infections in pangolins.

Pulmonary Tuberculosis Mimicking Lung Cancer and Metastasis Radiologically- A Case Report

2020 ◽  
Author(s):  
Harveen Kaur
Keyword(s):  
Lung Cancer ◽  
Pulmonary Tuberculosis ◽  
Diagnostic Procedures ◽  
Mediastinal Lymphadenopathy ◽  
Presumptive Diagnosis ◽  
Clinical Presentations ◽  
High Incidence ◽  
Pulmonary Infiltration ◽  
Low Incidence ◽  
Clinical Conditions

Tuberculosis (TB) is known to mimic several clinical conditions, especially malignancy. Pulmonary TB can present with pulmonary infiltration with or without mediastinal lymphadenopathy. TB often gets misdiagnosed in the countries having a low incidence of TB with a high incidence of lung cancer and varying clinical presentations, which results in delay in treatment initiation and unnecessary diagnostic procedures. We present a case of a 52-year old female, with a presumptive diagnosis of malignancy, which was subsequently proved as pulmonary tuberculosis with no evidence of malignancy instead.

scholarly journals Study on incidence and pathology of gastrointestinal parasitic infections in Nilgai Boselaphus tragocamelus in Hisar, Haryana, India

2021 ◽  
Vol 13 (13) ◽  
pp. 20124-20127
Author(s):  
Maneesh Sharma ◽  
B.L. Jangir ◽  
D. Lather ◽  
G.A. Chandratre ◽  
V. Nehra ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Developmental Stages ◽  
Histopathological Examination ◽  
Point Of View ◽  
Parasitic Infections ◽  
Traumatic Injuries ◽  
Faecal Samples ◽  
Veterinary Pathology ◽  
Parasitic Load ◽  
Animal Sciences

The present study was conducted on 20 dead carcasses of Nilgai Boselaphus tragocamelus brought to the Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar for post mortem examination. Thorough necropsy examinations were conducted and the representative samples for parasitic examination were collected. Most of the carcasses showed varying degrees of traumatic injuries (9), external wounds and haemorrhages (5) along with putrefactive changes (6). Intestinal contents and faecal samples were collected and screened for the presence of any parasite by sedimentation and flotation techniques. Out of 20 faecal samples, overall incidence of parasitic gastrointestinal tract infection was 40% (8/20). Out of 20, eight cases revealed presence of coccidian oocysts, however, among the eight cases, one case also revealed mixed infection of Moneizia andStrongylespp.,andanother case with Strongyle and Trichuris spp. Gross pathology of the intestines revealed varying degrees of vascular changes like petechial haemorrhages and the presence of catarrhal exudate. Histopathological examination revealed mild to moderate congestion, fused villi, desquamated mucosal epithelium in focal areas, and infiltration of mononuclear cells mainly lymphocytes. The different developmental stages of coccidian spp.were also observed in the intestinal mucosa. In conclusion, the preliminary study reported the parasitic load and pathological changes in the intestinal tract which further indicated the parasitism of these wild animals which is of immense significance from the epidemiological point of view.

Abstract 20: Resolution Phenotype of Monocytes and Macrophages is Altered in Peripheral Arterial Disease

2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Melinda S Schaller ◽  
Laura Menke ◽  
Mian Chen ◽  
Warren J Gasper ◽  
S. Marlene Grenon ◽  
...  
Keyword(s):  
Peripheral Arterial Disease ◽  
Cell Surface ◽  
Phagocytic Activity ◽  
Mononuclear Cells ◽  
Gradient Centrifugation ◽  
Arterial Disease ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Peripheral Blood Mononuclear ◽  
Peripheral Arterial

Introduction: Peripheral arterial disease (PAD) is a chronic disease characterized by systemic inflammation. Monocytes (Mo) and macrophages play a central role in vascular inflammation and its resolution. We hypothesize that impaired resolution in PAD results in poor clinical outcomes. Methods: Resolution phenotype was assessed by phagocytic activity of leukocytes, Mo cell surface markers, and cytokine profiling of Mo-derived macrophages (MDM). Phagocytosis and cell-surface markers were determined by flow cytometry. MDMs were generated from peripheral blood mononuclear cells via density gradient centrifugation. Cytokines were measured by ELISA following MDM differentiation and subsequent stimulation with LPS. Results: Circulating Mo and neutrophils (PMN) isolated from PAD patients (n=9) demonstrated significantly lower phagocytic activity (Mo: >30%, p<.001; PMN: >25%, p<.01, Fig. 1) as compared to healthy subjects (HS) (n=14). Cell-surface marker analysis demonstrated a higher proportion of the pro-inflammatory intermediate Mo subset (CD14 ++ 16 + , 1.8-fold, p=.04) in PAD compared to HS. MDM from PAD subjects retain their intrinsic inflammatory program by producing more IL-6 (PAD 3138±2676 ng/mL, HS 731±854 ng/mL p=.03) and IL-1β (PAD 244±236 ng/mL, HS 24.1±23.8 ng/mL p=.04) than those from HS. Upon stimulation with LPS, MDM from PAD subjects secrete more IL-6 (PAD 23353±22483 ng/mL, HS 5097±5836 ng/mL p=.05) than those from HS. Conclusions: Circulating Mo and PMN in patients with PAD have substantially lower phagocytic activity as well as a greater proportion of the pro-inflammatory intermediate Mo subset compared to HS. MDM preserve their elevated inflammatory state throughout culture and retain a heightened response upon latter stimulatory cues. Collectively these data demonstrate a heightened inflammatory and impaired resolution phenotype in PAD that has potential implications for disease progression and response to interventions.

Reduced Expression of Annexin A2 is Associated with Impaired Cell Surface Fibrinolysis and Venous Thromboembolism

Blood ◽  
2021 ◽  
Author(s):  
Hannah Fassel ◽  
Huigen Chen ◽  
Mary Ruisi ◽  
Neha Kumar ◽  
Maria T DeSancho ◽  
...  
Keyword(s):  
Risk Factor ◽  
Venous Thromboembolism ◽  
Cell Surface ◽  
Mononuclear Cells ◽  
Annexin A2 ◽  
Clot Lysis ◽  
Mrna Levels ◽  
Peripheral Blood Mononuclear ◽  
Intravascular Thrombosis

Reduced plasma fibrinolysis has been identified as a potential risk factor for venous thromboembolism (VTE), but the role of cell surface fibrinolysis in VTE is unknown. The annexin A2/S100A10 complex serves as a co-receptor for plasminogen and tissue plasminogen activator (tPA), augmenting plasmin generation by 60-fold on the endothelial cell surface. Several studies in both mice and humans support the concept that A2 regulates fibrin homeostasis and intravascular thrombosis in vivo. Here, we examined A2 protein expression and function in 115 adult subjects with venous thromboembolism (VTE) and 87 healthy controls. Using peripheral blood mononuclear cells (PBMCs) as a surrogate for endothelial cells, we found a 41% mean decrease in cell surface tPA-dependent fibrinolytic activity in subjects who had a positive personal and family history of VTE, but tested negative for known inherited thrombophilias. A2 protein was reduced on average by 70%, and mRNA levels by 30%, but neither decrease correlated with anticoagulant therapy. [Sentence omitted] Neither cell A2 protein nor cell surface plasmin generation correlated with plasma-based clot lysis times, suggesting that the plasma and cell surface fibrinolytic systems operate independently of one another. These data suggest that reduced expression of annexin A2 protein is associated with cell surface hypofibrinolysis and may represent a novel risk factor for inherited thrombophilia.

Cell surface expression of LDL receptor in chronic hepatitis C: correlation with viral load

2007 ◽  
Vol 293 (1) ◽  
pp. E416-E420 ◽  
Author(s):  
Jean-Michel Petit ◽  
Anne Minello ◽  
Laurence Duvillard ◽  
Valérie Jooste ◽  
Serge Monier ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chronic Hepatitis ◽  
Viral Load ◽  
Hepatitis C ◽  
Cell Surface ◽  
Chronic Hepatitis C ◽  
Mononuclear Cells ◽  
Ldl Receptor ◽  
Surface Expression ◽  
Cell Surface Expression

The LDL receptor (LDL-R) has been proposed as the viral receptor for Hepatitis C virus (HCV). This hypothesis has been based exclusively on in vitro studies. In human mononuclear cells, LDL-R gene expression has been demonstrated to be parallel and be coordinately regulated to gene expression in the human liver. The purpose of the current study was to determine the mononuclear cell surface expression of the LDL receptor in patients with HCV chronic infection according to viral load. Sixty-eight consecutive untreated chronic hepatitis C patients were studied to determine the mononuclear cell surface expression of the LDL-R. LDL-Rs were quantified at the surface of mononuclear cells in fresh blood samples taken after fasting using flow cytometry. LDL-R expression was significantly associated with LDL-cholesterol ( r = −0.25; P = 0.03) and HCV-viral load ( r = 0.37, P = 0.002). In multivariate analysis, the LDL-R expression was significantly associated with HCV viral load, whereas genotype, age, body mass index, and fibrosis were not. In conclusion, our data provided by a human study, suggest that the LDL-R may be one of the receptors implicated in HCV replication.

SuPAR, an emerging biomarker in kidney and inflammatory diseases

2018 ◽  
Vol 94 (1115) ◽  
pp. 517-524 ◽  
Author(s):  
Lamiaa Hamie ◽  
Georges Daoud ◽  
Georges Nemer ◽  
Tarek Nammour ◽  
Alissar El Chediak ◽  
...  
Keyword(s):  
Cell Surface ◽  
Plasminogen Activator ◽  
Large Scale ◽  
Inflammatory Diseases ◽  
Cell Surface Receptor ◽  
Urokinase Plasminogen Activator Receptor ◽  
Surface Receptor ◽  
Treatment Plans ◽  
Plasminogen Activator Receptor ◽  
Clinical Conditions

Soluble urokinase plasminogen activator receptor (suPAR) is a circulating form of a physiological and pathophysiological important cell surface receptor, implicated in inflammation. Recent studies showed that suPAR is a promising biomarker, useful for diagnosis, assessment and prognosis of several diseases. This review summarises the majority of preliminary studies and analyses the significance and the clinical application of suPAR in various clinical conditions. SuPAR seems to have a significant value in the diagnosis as well as prognosis of many diseases; nonetheless, it merits large-scale studies to set cut-off values that help physicians in following up their patients and accordingly tailor their treatment plans.

scholarly journals Detection of empty HLA class II molecules on cord blood B cells

Blood ◽  
1996 ◽  
Vol 87 (9) ◽  
pp. 3970-3976 ◽  
Author(s):  
F Garban ◽  
M Ericson ◽  
C Roucard ◽  
C Rabian-Herzog ◽  
H Teisserenc ◽  
...  
Keyword(s):  
B Cells ◽  
Cell Surface ◽  
Cord Blood ◽  
Mixed Lymphocyte Reaction ◽  
Mononuclear Cells ◽  
Sodium Dodecyl ◽  
Class Ii ◽  
Hla Class Ii ◽  
High Level ◽  
Hla Class Ii Molecules

Fetal mononuclear cells are increasingly used in transplantation of hematopoietic cells due to a reportedly lower incidence of graft-versus- host disease. Previous studies of immune responses of fetal lymphocytes have indicated a general hyporesponsiveness in response to polyclonal stimulation. Fetal B lymphocytes display many features typical of the resting state such as a low level of HLA class II expression, but a large proportion of cells also carry the activation-associated CD23 antigen. We show here that despite a low cell surface level of all three HLA class II isotypes on fetal B cells, their allogeneic capacity, measured as the ability to elicit a mixed lymphocyte reaction, is similar to that of adult B cells. Allogeneic stimulation is believed to be peptide-dependent. Surprisingly, the majority of the HLA class II molecules on cord blood B cells appeared to be devoid of stably bound peptide as detected by the binding of a recombinant and soluble invariant chain, as well as by the absence of sodium dodecyl sulfate (SDS) stable alpha beta heterodimers in whole cell lysates. Immunoblot experiments showed that HLA class II molecules of fetal B cells were predominantly present in high molecular weight aggregates in stark contrast to B cells of adult origin. However, a sensitive cell surface labeling technique followed by immunoprecipitation enabled us to detect an SDS-stable 120-kD molecule on fetal B cells. We propose that the 120-kD molecules could correspond to HLA class II doubledimers or superdimers. We hypothesize that the 120-kD HLA class II molecule functions as the antigen-presenting molecule in the mixed lymphocyte reaction of fetal B cells, as it is the major species detected on the surface. Secondly, we suggest that a high level of empty HLA class II molecules may be indicative of a particular stage in B-cell ontogeny.

scholarly journals Seroprevalence and pathological study of Salmonella infections in layer chickens and isolation and identification of causal agents

1970 ◽  
Vol 4 (2) ◽  
pp. 79-85 ◽  
Author(s):  
MM Islam ◽  
MG Haider ◽  
EH Chowdhury ◽  
M Kamruzzaman ◽  
MM Hossain
Keyword(s):  
Mononuclear Cells ◽  
Research Work ◽  
Liver Parenchyma ◽  
Muscularis Mucosa ◽  
Pathological Study ◽  
Isolation And Identification ◽  
Bacterial Colony ◽  
Focal Necrosis ◽  
Salmonella Infections ◽  
Layer Chickens

The present research work was conducted to study about the seroprevalence and pathology of Salmonella infections in layer chickens of Dhaka and Gazipur regions of Bangladesh and to isolate and characterize Salmonellae from layer chickens during the period from January to May 2006. The used materials were blood sample, cloacal and liver swabs from live and dead birds respectively, and visceral organs (liver, lungs, spleen and intestine). The used methods were serum plate agglutination (SPA) test; necropsy and histopathology; cultural, morphological and biochemical test. The overall seroprevalence was 43.4%. During necropsy, congested and enlarged liver with focal necrosis, haemorrhagic and discoloured ovary with stalk formation and mild haemorrhagic to catarrhal enteritis in intestine were detected. Microscopically, the liver showed congestion, focal necrosis with multifocal infiltration of histiocytes in liver parenchyma. In ovum, infiltration of leukocytes, bacterial colony, RE cell proliferation and fibrinoid necrosis were recorded. The intestinal mucosa exhibited infiltration of mononuclear cells in mucosa, submucosa as well as muscularis mucosa. A total of 33 (21.02%) Salmonellae from live and dead birds were isolated. The isolation rate of Salmonellae was higher in seronegative (31.6%) group than seropositive (3.2%) group. Out of 33 Salmonella isolates, 25 were S. pullorum, 3 were S. gallinarum and the rest 5 were motile Salmonellae. The isolates obtained in the study may be investigated for serotyping, pathogenesis study, antibiogram and vaccine production in future. Key words: Seroprevalence, histopathology, isolation, identification, Salmonella, chickensDOI = 10.3329/bjvm.v4i2.1288Bangl. J. Vet. Med. (2006). 4 (2): 79–85

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