scholarly journals Evaluation of Antidiabetic Activity Mechanism of Ethanol Extract of Alangium salvifolium Leaves Employing Cell-based Assays

2019 ◽  
Vol 12 (04) ◽  
pp. 130-133
Author(s):  
Mayank Singh Lodhi ◽  
Balkrishna Dubey ◽  
Ashish Manigauha
Keyword(s):  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Activity Mechanism

scholarly journals Antihyperglycemic activity of Centella asiatica (L.) Urb. leaf ethanol extract SNEDDS in zebrafish (Danio rerio)

2021 ◽  
Vol 19 (1) ◽  
pp. 184-188
Author(s):  
Farida Hayati ◽  
Lutfi Chabib ◽  
Faiza Dea Sekarraras ◽  
Wan Syarifah Faizah
Keyword(s):  
Glucose Solution ◽  
Fasting Blood Glucose ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Tween 80 ◽  
Positive Control ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Peg 400 ◽  
Good Preparation

Abstract This study aimed to identify the effectiveness of SNEDDS of Pegagan Leaf Ethanol Extract (PLE) to reduce fasting blood glucose (FBG) levels in zebrafish. Centella asiatica (L.) Urb. or pegagan is among the medicinal plants widely used to treat diabetes in Indonesia. Maceration was employed with 70% ethanol to obtain a viscous extract for the formulation of SNEDDS with Capryol 90, Tween 80, and PEG 400 (1:6:3). Antihyperglycemic testing was conducted on five groups, consisting of normal, positive control, negative control, P I treatment, and P II treatment. On Day 1, all except the normal group was induced with 300 mg alloxan and soaked in 2% glucose solution for 7 days. On day 8, the treatment consisted of 25 mg/2 L metformin for the positive control, 100 mg/2 L SNEDDS for P I, 200 mg/2 L SNEDDS for P II, and no treatment for the negative control. The SNEDDS characterization obtained 100.6 ± 3.12 nm particle size and −7.93 ± 0.66 mV zeta potential, indicating that the SNEDDS had fulfilled the requirements of good preparation. The antidiabetic activity test found a 69.90% decline in FBG levels in 100 mg/2 L SNEDDS and 72.20% in 200 mg/2 L SNEDDS.

scholarly journals Antidiabetic and anti-obesity acylated flavonol diglucoside from Ammannia baccifera L. subsp. aegyptiaca (Willd.) Koehne Waste

2020 ◽  
Author(s):  
Noha Swilam ◽  
Mahmoud Nawwar ◽  
Rasha Radwan ◽  
Eman Mostafa
Keyword(s):  
Binding Free Energy ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Insulin Level ◽  
Diabetic Rats ◽  
High Serum ◽  
Antidiabetic Activity ◽  
Active Site Residues ◽  
Sod Activity ◽  
Aerial Parts

Abstract Chemical investigation of the aerial parts of Ammania aegyptiaca ethanol extract (AEEE) revealed significant high concentrations of polyphenols and flavonoids content with notable antioxidant activity in DPPH, ORAC, and reducing power assay. New acylated diglucoside flavonol myricetin 3-O-β-4­C1-(6"-O-galloyl glucopyranoside) 7-O-β-4C1-glucopyranoside (MGGG) was isolated from aerial parts of AEEE along with four additional known phenolics, not characterized previously from AEEE. Moreover, powerful inhibitory effects of MGGG, AEEE, and all isolates against α-amylase, pancreatic lipase and β-glucosidase, were assessed. In addition, flexible molecular docking was used to reveal the inhibition towards digestive enzymes and confirmed that the MGGG interacted strongly with the active site residues of these enzymes with the highest binding free energy against β-glucosidase (DG=-8.98 kcal/mol) compared to the commercial drug Acarbose, thus justifying its dual management of diabetes and obesity. In streptozotocin (STZ) induced diabetic rats, AEEE significantly decreased high serum glucose, α-amylase activity, liver and kidney function markers and increased insulin level. Moreover, it improved lipid profile due to diabetes with increased SOD activity and inhibited of TBARS formation. Consequently, AEEE and MGGG are found useful in controlling the secondary complications associated with type 2 diabetes mellitus. Histopathological studies proved the decrease in the pancreas damage and agreed with the biochemical findings. These results provide evidence that AEEE and MGGG have potent antidiabetic activity, which warrants additional investigations.

scholarly journals Antidiabetic Activities and Identification of Chemical Compound from Samama (Anthocephalus macrophyllus (Roxb) Havil)

2018 ◽  
Vol 18 (1) ◽  
pp. 66 ◽  
Author(s):  
Laela Nur Anisah ◽  
Wasrin Syafii ◽  
Gustan Pari ◽  
Rita Kartika Sari
Keyword(s):  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antidiabetic Activity ◽  
Isopropyl Myristate ◽  
Phytochemical Analysis ◽  
Triterpenoid Saponin ◽  
Ic50 Value ◽  
High Contribution ◽  
Ethanol Extracts

Samama (Anthocephalus macrophyllus) is one of a pioneer indigenous fast growing species in Indonesia which have been used as traditional medicine for various diseases. The objectives were to determine the yield extract, to analyze their antidiabetic activity by inhibition assay for α-glucosidase enzyme activity and chemical analysis with GCMS. Extraction of leaves, bark and wood samples were done by using ethanol 95%. Fractionation the most active  ethanol extract was conducted by using n-hexane and ethyl acetate. The yield of ethanol extracts in leaves, bark and wood were 13.90%, 12.87%, and 2.18% respectively. Based on antidiabetic activity assay, the bark ethanol extract was the most active extract by the IC50 value of 5.86 μg mL-1. Phytochemical analysis on bark ethanol extracts showed that they contained flavonoid, quinon, triterpenoid, saponin and tannin which were assumed have high contribution in antidiabetic activities. The result of fractionation ethanol extract bark showed that the ethyl acetate fraction was the most active fraction (IC50 6.82 μg mL-1). GCMS analysis indicated the presence of dominant phenolic compounds such as pyrocatechol, antiarol, isopropyl myristate and phenol in which were suspected have antidiabetic activity. These results strongly suggested that ethyl acetate fraction of Samama bark was a potential natural source for antidiabetic agents.

Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

2019 ◽  
Vol 13 (01) ◽  
pp. 27-33
Author(s):  
Evi Sovia ◽  
Dian Anggraeny ◽  
Ris Kristiana ◽  
Firhan Hamdi Maulida ◽  
Mutia Susparini
Keyword(s):  
Blood Glucose ◽  
Histological Examination ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetes Mice ◽  
Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

scholarly journals ANTIDIABETIC ACTIVITY OF BAUHINIA VAHLII Wt. and Arn. (CAESALPINIACEAE) ROOT – A BOTANICAL SOURCE FOR THE AYURVEDA DRUG MURVA

2019 ◽  
pp. 359-362
Author(s):  
KAMATCHI SUNDARA SARAVANAN ◽  
VARADHARAJAN MADHAVAN
Keyword(s):  
Diabetes Mellitus ◽  
Biochemical Parameters ◽  
Histological Analysis ◽  
Reduced Glutathione ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Serum Triglycerides ◽  
Standard Drug ◽  
Acute Toxicity Study

Objective: The objective of this study was to evaluate the antidiabetic potential of Bauhinia vahlii Wt. and Arn. (Caesalpiniaceae) root, a botanical source for Murva (Ayurveda drug). Methods: Ethanol extract of B. vahlii root (EEBVR) and aqueous extract of B. vahlii root (AEBVR) prepared were subjected for acute toxicity study adopting Organisation for Economic Cooperation and Development guidelines. Antidiabetic property of EEBVR and AEBVR was screened against Streptozotocin-nicotinamide-induced Diabetes Mellitus (DM). The diabetic animals were administered with standard drug glibenclamide (0.5 mg/kg), EEBVR (200 and 400 mg/kg), and AEBVR (200 and 400 mg/kg) for 21 days. Fasting blood glucose, serum triglycerides, total cholesterol, liver malondialdehyde, reduced glutathione, and glycogen were estimated along with pancreatic histological analysis. Results: EEBVR (400 mg/kg) exerted a marked antidiabetic activity among the extracts at the tested doses, as evidenced by considerable reversal of biochemical parameters that were well supported by the histopathological interpretation of pancreas. Conclusion: This study confirms the antidiabetic potential of B. vahlii root and also its traditional claim in the use of DM.

scholarly journals Antidiabetic Activity ofPterospermum acerifoliumFlowers and Glucose Uptake Potential of Bioactive Fraction in L6 Muscle Cell Lines with Its HPLC Fingerprint

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Rathinavelusamy Paramaguru ◽  
Papiya Mitra Mazumder ◽  
Dinakar Sasmal ◽  
Venkatesan Jayaprakash
Keyword(s):  
Glucose Uptake ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diabetic Control ◽  
Antidiabetic Activity ◽  
Peripheral Tissues ◽  
Hplc Fingerprint

The present study was designed to estimate the detailed antidiabetic activity ofPterospermum acerifolium(L.) Willd flowers.In vitroalpha amylase inhibition study was carried out on 50% ethanol extract of flowers (PAFEE) and its various fractions. The active ethyl acetate fraction (PAFEF) was subfractionated into three subfractions (PAFE1, PAFE2, and PAFE3) and subjected to acute toxicity studies followed by antidiabetic screeningin vivoby streptozotocin-nicotinamide induced type II diabetes. Diabetic animals treated with PAFE2 (30 mg/kg) reduced the levels of fasting blood glucose, significantly (P<0.001) compared to that of diabetic control animals. Histological studies on drug treated groups did not show remarkable positive changes inβ-cells. PAFE2 showed32.6±1.93% glucose uptake over control and, in the presence of PI3K inhibitor wortmannin, declined to13.7±2.51%. HPLC analysis of PAFE2 reveals the presence of quercetin and apigenin as major constituents and both are inhibiting the glycogen phosphorylase enzyme in molecular modelling studies. The study evidenced strongly that the probable glucose lowering mechanism of action of active subfraction PAFE2 is by increasing the glucose uptake in peripheral tissues and by inhibition of gluconeogenesis.

scholarly journals Evaluation the Effect of Vernonia amygdalina. Del Leaves Ethanol Extract on Blood Sugar and its Synergism with Glibenclamide in Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 26-34
Author(s):  
Igwe K ◽  
Ikpeazu O ◽  
Otuokere I
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Treatment Groups ◽  
Glucose Levels

Antidiabetic activity of Vernonia amygdalina and its possible synergism with glibenclamide was checked. Forty eight rats were used for the research, for hypoglycermic study of V. amygdalina alone, they were grouped into five of six rats each. Group 1 was the negative control and was administered distilled water orally. Groups 2, 3, and 4 were the treatment groups which received 100, 200 and 300 mg/kg body weight of the V. amygdalina extract respectively orally by intubation. Group 5 was the positive control group which received a known antidiabetic drug, glibenclamide. Diabetes was induced with alloxan. For the synergism study, another 18 rats grouped into 3 of six rats each was used. Both groups of glibenclamide only and glibenclamide plus V. amygdalina extract were dosed for 14 days orally by intubation, thereafter were sacrificed and blood collected from heart for analysis. There were 5 replicates grouped by weight throughout the study and both single and synergistic studies had the same controls. Effect of V. amygdalina extract was checked on blood glucose and its possible synergism with glibenclamide. All results in treatment groups were compared with the normal control at statistical confidence of p<0.05. Result shows that V. amygdalina extract reduced blood glucose level in the test groups as dose of extract increased. Combination of V. amygdalina with glibenclamide demonstrated further deduction in blood glucose levels in the treatment rats groups. Therefore addition of V. amygdalina into glibenclamide increased efficacy in the diabetic rats. The interaction between V. amygdalina and glibenclamide in this work was additive and therefore synergistic.

scholarly journals IN VIVO ANTIDIABETIC SCREENING OF KABAU (ARCHIDENDRON BUBALINUM (JACK) I. C NIELSEN) SEEDS

2021 ◽  
pp. 228-234
Author(s):  
HESTI RIASARI ◽  
NOVI IRWAN FAUZI ◽  
KUSNANDAR ANGGADIREDJA ◽  
RIKA HARTATI ◽  
SUKRASNO
Keyword(s):  
Blood Sugar ◽  
Screening Method ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
High Fat ◽  
Methanol Fraction ◽  
Glucose Test ◽  
Sugar Levels ◽  
Tolerance Method

Objective: Study described the screening potential antidiabetic activity of kabau seed extract and fraction. Methods: The powdered crude drugs weighing 1349.32 grams were extracted with a solvent with solvents with escalating polarity by using soxhletation. The solvents used were n-hexane, ethyl acetate, and 96% ethanol. Screening activity using three variations on doses on the three extracts using the glucose test tolerance method, then the alloxan induction and high-fat feed induction testing methods using selected doses, decreasing blood glucose levels using the GOD PAP enzyme and decreasing MDA levels and increased levels of the enzyme SOD. Extracts that have potential antidiabetic activity are fractionated using liquid-solid fractionation; then the fraction is screened for antidiabetic activity using the glucose test tolerance method. Results: Screening for antidiabetic activity on the three extracts using the glucose test tolerance method showed that the ethanol extract at a dose of 250 mg/kg BW. The three extracts were then screened for the next mechanism using the alloxan induction method and high-fat feed induction, the decrease in blood sugar levels by the GOD-PAP method showed a good decrease in the ethanol extract by 202.94±2 mg/dl, the three extracts showed good less significant, in the SOD enzyme method, the ethanol extract gave a good value such as the positive control value. Testing on fraction can decrease in blood sugar; the results showed that the ethanol extract and methanol fraction gave a small AUC 0-150 (32695,3 and 33167,71), where the value was close to the result of the glibenclamide 30238,48. Conclusion: The antidiabetic activity of the extract showed that the ethanol extract was better with the glucose test tolerance method, with alloxan induction animal models and high-fat feed induction. In the methanol fraction derived from 96% ethanol extract, it provides a good reduction in blood sugar levels in the screening method with a glucose test tolerance

scholarly journals Aktivitas In Vitro Penurun Gula Darah dari Ekstrak Buah Parijoto (Medinilla speciosa B.)

2020 ◽  
Vol 31 (1) ◽  
pp. 31
Author(s):  
Rissa Vifta ◽  
NFN Wilantika ◽  
Yustisia Dian Advistasari
Keyword(s):  
Block Design ◽  
Enzymatic Reaction ◽  
Ethanol Extract ◽  
Complementary Therapy ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Flavonoid Compounds ◽  
Treatment And Prevention ◽  
Ethanol Extracts

Diabetes mellitus is one of the highest causes of death in the world, with symptoms of increased blood glucose levels (hyperglycemia). One of the efforts made as a treatment and prevention of DM is through complementary therapy using natural ingredients. Parijoto fruit (<em>Medinilla speciosa</em>) contains flavonoid compounds potential to reduce glucose levels. The study aimed to analyze the effectiveness of ethanol extracts and its fractions of parijoto fruit (EEBP) to decrease glucose levels in vitro. The study was conducted at the Ngudi Waluyo University Laboratory in April to August 2018. Identification and screening of flavonoid compounds from ethanol extracts and its fractions of parijoto fruit were performed qualitatively and by thin-layer chromatography (TLC). <em>In vitro</em> testing for  antidiabetic activity of EEBP was performed with non-enzymatic reaction by Nelson Somogyi method (ethanol extract and its fractions). Experiment  was arranged in a randomized block design, with 12 treatments and three replications. The concentrations of parijoto fruit tested were 10 ppm, 20 ppm, 30 ppm, 40 ppm, 50 ppm, and 60 ppm. The results of qualitative identification and TLC showed that the ethanol extract and its fraction of EEBP contained flavonoid compounds. At a concentration of 30 ppm, EEBP was able to reduce glucose levels by 42.43 %, while the ethanol fraction decreased glucose levels by 83.38 %. The results showed parijoto fruit ethanol extract and its fractions were sources of antidiabetic. Hence its pharmacologically effect was necessary to be further studied.

scholarly journals UJI AKTIVITAS EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) SEBAGAI INHIBITOR ENZIM α-GLUKOSIDASE DENGAN MENGGUNAKAN ELISA READER

2020 ◽  
Vol 7 (2) ◽  
pp. 1-5
Author(s):  
Sukmawati Syarif ◽  
Nurnaningsih Nurnaningsih ◽  
Mamat Pratama
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sample Extract ◽  
Male Wistar Rats ◽  
Muntingia Calabura

Kersen leaves (Muntingia calabura L) contain metabolites such as alkaloids, anthrax, polyphenols, tannins, saponins and are rich in flavonoid components such as flavones, flavonones, flavan and biflavan which have antidiabetic activity. According to a study conducted by Apriyanti (2016), the ethanol extract of kersen (Muntingia calabura L) at a dose of 250 mg / kg BB significantly reduced blood glucose levels in male wistar rats. In this study aimed at determining IC50 ethanol extract of Muntingia calabura L as an inhibitor of α-glucosidase enzymes using ELISA reader. The method was divided into 3 category is 1 (sample extract), 2 (blank) and 3 (akarbose). Each group added 25 µL α-glucosidase solution (0.25 units / mL then measured using ELISA reader 405 nm. The results showed that the ethanol extract of kersen (Muntingia calabura L) had activity as an inhibitor of α-glucosidase enzyme with IC50 34,197 µg / mL and can be categorized as active.

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