scholarly journals In vivo study of the antioxidant test of ethanolic extract of Chromolaena odorata Linn. leaves

MEDISAINS ◽  
2020 ◽  
Vol 18 (3) ◽  
pp. 86
Author(s):  
Indah Solihah ◽  
Herlina Herlina ◽  
Inayatul Munawwaroh ◽  
Riana Sari Puspita Rasyid
Keyword(s):  
Antioxidant Activity ◽  
Serum Level ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Control Treatment ◽  
P Value ◽  
Histological Structure ◽  
Chromolaena Odorata ◽  
Male Wistar Rats

Background: Chromolaena odorata L. leaf was reported to contain phenolic group compounds, such as flavonoids. Flavonoid compounds have been reported to have antioxidant activity. Based on in vitro study, Chromolaena odorata L. leaves have potent antioxidant activity. However, in vivo, studies with dose variations have not been reported.Purpose: This study evaluates the antioxidant activity with various doses of ethanolic extract of Chromolaena odorata L. leaves against male Wistar rats induced by paracetamol.Methods: Flavonoid contents were measured spectrophotometrically based on the formation of a complex flavonoid-aluminum. Quercetin was used to make a calibration curve. In vivo test was used TBARS method carried out by measured malondialdehyde (MDA) level in male Wistar rats induced by paracetamol 2g/Kg BW. The test was carried out on extracts with doses of 125, 250, and 500 mg/Kg BW. Vitamin C with dose 6,5mg/Kg BW used as a positive control, and 1% of Na CMC used as the negative control. Histopathology assessment of liver used Hema-toxylin Eosin Stain.Results: Ethanolic extract of Chromolaena odorata L. leaves contain flavonoid 126.459±0.163 mg/g extract as quercetin equivalent. Intoxication paracetamol on rats increased MDA serum level significantly different (p-value < 0.005) with normal control. Treatment of ascorbic acid and extracts decreased MDA serum level significantly different (p-value < 0.005) with control negative and improved the histological structure of hepatocytes.Conclusion: Ethanolic extract of Chromolaena odorata Linn. dose 500 mg/Kg BW was the best treatment with exhibited 58.974% reduction of MDA serum level and better improve the histological structure hepatocytes than other doses

Anti-diabetogenic and in vivo antioxidant activity of ethanol extract of Dryopteris dilatata in alloxan-induced male Wistar rats

Biomarkers ◽  
2021 ◽  
pp. 1-15
Author(s):  
Akpotu E. Ajirioghene ◽  
Samuel I. Ghasi ◽  
Lawrence O. Ewhre ◽  
Olusegun G. Adebayo ◽  
Jerome N. Asiwe
Keyword(s):  
Antioxidant Activity ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Male Wistar Rats

scholarly journals In vivo antioxidant activity of Ethanolic extract from root of Smilax zeylanica on Aluminium Chloride Induced oxidative stress in Wistar rats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 48-52
Author(s):  
B. Sabari Senthil ◽  
V.K. Kalaichelvan ◽  
A. Kottai Muthu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Body Weight ◽  
Wistar Rats ◽  
Reduced Glutathione ◽  
Ethanolic Extract ◽  
Aluminium Chloride ◽  
Control Group

Objective: The objective of the present study was to investigate the Evaluation of In vivo antioxidant activity of Ethanolic extract of root of Smilax zeylanica(EESZ) on Aluminium Chloride Induced apoptosis suppressing oxidative stress  in Wistar rats. Materials and Methods: The ethanolic extract from the roots of S. china by hot continuous percolation method. The rats were divided into 5 groups and each group consists of 6 animals. Rats were treated with EESC for 150 and 300 mg/ kg of body weight and piracetam, 0.5 mg/ kg of body weight for 14 successive days after inducing oxidative stress  with aluminium chloride (100 mg/ kg of body weight) for 60 days. The lipid peroxidation level (TBARS) and antioxidant activities like Superoxide dismutase (SOD), Catalase (CAT) and reduced Glutathione (GSH) were estimated in rats. Results: AlCl3 induced rats showed increased the TBARS and decreased the antioxidant enzymes like Superoxide dismutase (SOD), Catalase (CAT) and reduced Glutathione (GSH) when compared with the control group. The EESZ at higher dose 300 mg/ kg of body weight animals were significantly (P < 0.001) reduced the TBARS and increased the anti oxidant enzymes Superoxide dismutase (SOD), Catalase (CAT) and reduced Glutathione (GSH) when compared with the AlCl3 treated group Conclusion: Findings of the present study revealed that Ethanolic extract from roots of Smilax zeylanica  may be used as a significant source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.                    Keywords: S. zeylanica, antioxidant, ethanolic extract, TBARS, rats.

Potency of Black Soybean (Glycine max L. Merr) and Jati Belanda Leaves (Guazuma ulmifolia Lamk) for Dyslipidemia Treatment In Vivo

2016 ◽  
Vol 1 (3) ◽  
Author(s):  
Sijani Prahastuti ◽  
Meilinah Hidayat ◽  
Michael W. Kurniadi ◽  
Selvina Christiany
Keyword(s):  
Wistar Rats ◽  
Ldl Cholesterol ◽  
Ethanolic Extract ◽  
Alternative Medicines ◽  
Equivalent Effect ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Glycine Max L ◽  
Potential Alternative

Soybean Detam 1 and Jati Belanda leaves are potential alternative medicines for treating dyslipidemia. The purpose of this study is to explore  the potential of the combination of Ethanolic Extract Soybean Detam 1 (EEKD) and Jati Belanda (EEJB) in lowering LDL cholesterol and triglycerides compared to single extract and Simvastatin. Using a real laboratory experimental method with CRD, a total of 36 male Wistar rats were divided into 6 groups and administrated for treatment of 28 days. The results showed a significant decrease of LDL cholesterol as well as triglyceride in NC  as compared to P1, P2, P3 (p <0.05) and no significant difference with PC (p> 0.05). The highest suppression of LDL cholesterol between P1 with P2 and P3 is (p <0.05) while the triglyceride is between P1 and P2 (p <0.05). This study concludes that the combination of EEKD 10 mg, 20 mg EEJB, EEKD 30 mg and EEJB 30 mg is significantly lowering LDL cholesterol and triglyceride. The combination of EEKD 10 mg, 20 mg EEJB is the highest compression of LDL cholesterol and triglyceride, and has an equivalent effect with simvastatin in lowering LDL cholesterol and triglycerides in male Wistar rats induced with high fat diet. Keywords: LDL cholesterol, triglycerides

scholarly journals Effects of systemic glycine on accumbal glycine and dopamine levels and ethanol intake in male Wistar rats

2020 ◽  
Author(s):  
Yasmin Olsson ◽  
Helga Höifödt Lidö ◽  
Klara Danielsson ◽  
Mia Ericson ◽  
Bo Söderpalm
Keyword(s):  
Wistar Rats ◽  
In Vivo Microdialysis ◽  
Ethanol Intake ◽  
Water Model ◽  
Glycine Transporter 1 ◽  
Improved Outcomes ◽  
Male Wistar Rats ◽  
Treatment Principle ◽  
Reward Pathway

AbstractApproved medications for alcohol use disorder (AUD) display modest effect sizes. Pharmacotherapy aimed at the mechanism(s) by which ethanol activates the dopamine reward pathway may offer improved outcomes. Basal and ethanol-induced accumbal dopamine release in the rat involve glycine receptors (GlyR) in the nucleus accumbens (nAc). Glycine transporter 1 (GlyT-1) inhibitors, which raise extracellular glycine levels, have repeatedly been shown to decrease ethanol intake in the rat. To further explore the rational for elevating glycine levels in the treatment of AUD, this study examined accumbal extracellular glycine and dopamine levels and voluntary ethanol intake and preference in the rat, after systemic treatment with glycine. The effects of three different doses of glycine i.p. on accumbal glycine and dopamine levels were examined using in vivo microdialysis in Wistar rats. In addition, the effects of the intermediate dose of glycine on voluntary ethanol intake and preference were examined in a limited access two-bottle ethanol/water model in the rat. Systemic glycine treatment increased accumbal glycine levels in a dose-related manner, whereas accumbal dopamine levels were elevated in a subpopulation of animals, defined as dopamine responders. Ethanol intake and preference decreased after systemic glycine treatment. These results give further support to the concept of elevating central glycine levels to reduce ethanol intake and indicate that targeting the glycinergic system may represent a pharmacologic treatment principle for AUD.

scholarly journals RADIOLOGICAL STUDY OF MEGACOLON IN TRYPANOSOMA CRUZI INFECTED RATS

2018 ◽  
Vol 31 (1) ◽  
Author(s):  
Carlos Edmundo Rodrigues FONTES ◽  
Ana Paula de ABREU ◽  
Aretuza Zaupa GASPARIM
Keyword(s):  
Trypanosoma Cruzi ◽  
Wistar Rats ◽  
In Vivo Studies ◽  
Proposed Model ◽  
Male Wistar Rats ◽  
Group A ◽  
Time Of Infection ◽  
Group B ◽  
Digital Caliper

ABSTRACT Background: Researches on Chagas disease still use several animals and rats, due to size and susceptibility were preferred by many authors. Aim: To develop an experimental model of megacolon in rats inoculated with the strain Y of Trypanosoma cruzi. Methods: Thirty male Wistar rats were distributed in three groups inoculated with different inoculants: Group A: 600000, Group B: 1000000 and Group C: 1500000 blood trypomastigotes of T. cruzi. Animals were sedated intramuscularly at zero inoculation time (T0) and 60 days after inoculation (T60), to perform the barium enema in order to evaluate the dilatation of the different segments of colon in a comparative study of the measurements obtained, using a digital caliper. Evidence of infection was performed by blood smear collected from the animal’s tail 18 days after inoculation with observation of blood forms. Results: Comparing the intestinal diameter of the inoculated animals with 60,0000 trypomastigotes in the T0 of infection with T60 days after the inoculation, significant dilatation was observed between the proximal, medial and distal segments (p<0.01), indicating the establishment of the megacolon model. In addition, comparing intestinal diameter between the different segments, with in the T0 of infection and the T60 after inoculation, significant alterations were observed (p<0.05). Conclusion: The proposed model was possible for in vivo studies of alterations due to infection by T. cruzi and functional alterations of the colon. In addition, the changes manifested in the colon are not directly proportional to the size of the inoculum, but to the time of infection that the animals were submitted, since the animals inoculated with 60,0000 blood forms were the ones which presented the most significant alterations.

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

scholarly journals Evaluation of analgesic and anti-inflammatory activity of ethanolic extract of Enhydra fluctuans on male wistar rats

2021 ◽  
Vol 9 (1) ◽  
pp. 1-7
Author(s):  
Kishor Kumar Roy ◽  
Md. Kabirul Islam Mollah ◽  
Md. Masud Reja ◽  
Dibyendu Shil ◽  
Ranjan Kumar Maji
Keyword(s):  
Wistar Rats ◽  
Pain Threshold ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Hot Plate ◽  
Plate Method ◽  
Traditional System ◽  
Male Wistar Rats ◽  
Anti Inflammatory

Enhydra fluctuations an edible semi-aquatic vegetable plant are widely used in the traditional system of medicine. Ethanolic extract of Enhydra fluctuans was screened for analgesic & anti-inflammatory activity. Analgesic activity was evaluated by the hot plate method and anti-inflammatory activity was evaluated by formalin induce paw edema in Wistar rats (male). Ethanolic extract dose of 300 mg/kg reduce formalin induce paw inflammation and also increased the pain threshold in rats evidenced by hot plate method. The experimental results concluded that Enhydra fluctuans have significant analgesic and anti-inflammatory activity may due to flavonoid and phenolic compound content.

scholarly journals Effectiveness of Vitamin C and Vitamin E Antioxidant Combination on Caspase-3 Expression in Wistar White Rat Pulmonum Contusion

2020 ◽  
Vol 3 (1) ◽  
pp. 31-44
Author(s):  
Bermansyah ◽  
Gama Satria ◽  
Ahmad Umar
Keyword(s):  
Cell Death ◽  
Vitamin E ◽  
Vitamin C ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Cell Function ◽  
Pulmonary Contusion ◽  
Tnf Α ◽  
Male Wistar Rats

Introduction.Pulmonary contusions can cause a progressive inflammatory response. Activation of TNF-α cytokines and reactive oxygen species (ROS) can cause pulmonary cell death. Antioxidants can have the potential to neutralize ROS. The purpose of this study is to determine the effectiveness of antioxidant administration in maintaining pulmonary cell function in wistar rats that have been induced to experience pulmonary contusions through caspase-3 levels. Methods.This study was an in vivo experimental study conducted on thirty male wistar rats and divided into five groups (n = 6): control, pulmonary contusion + asthaxanthine 5 mg/kgBW, pulmonary contusion + vitamin C and E 50 mg/kgBW, pulmonary contusion + vitamin C and E 100 mg/kgBW, pulmonary contusion + vitamin C and E 200 mg/kgBW. The value of Caspase-3 is evaluated by the IHC. All data analyzes used SPSS 18. Results. Low doses of antioxidants have the potential to reduce pulmonary cell death in wistar rats induced by pulmonary contusions.Conclussion. Vitamin C and E effective to reduce polmonary cell death in pulmonary contusion.Keywords: antioxidants, vitamin C, vitamin E, pulmonary contusions animal model, apoptosis, caspase-3

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