scholarly journals Skinning Injury Responses in Sweetpotato

2021 ◽  
Vol 17 (1) ◽  
pp. 1-8
Author(s):  
Jollanda Effendy ◽  
Don R LaBonte ◽  
Darda Efendi ◽  
Nurul Khumaida ◽  
Gustaaf A Wattimena
Keyword(s):  
Stress Responses ◽  
Ipomoea Batatas ◽  
Root Weight ◽  
Storage Roots ◽  
Gene Expressions ◽  
Cell Wall Modification ◽  
Wound Site ◽  
Underdeveloped Countries ◽  
Postharvest Loss ◽  
Moisture Increase

In sweetpotatoes (Ipomoea batatas L. Lamb), the loss of skin from the surface of the storage roots is known as skinning injury. It is responsible for significant postharvest loss resulting from moisture increase and weight reduction, wrinkling, and susceptibility to pathogen attack. Reduced root weight by water loss is associated with a higher rate of rot predominantly occurred in the developing and underdeveloped countries which can count of 8-20% of postharvest loss. Plants have different adaptation to protect themselves against skinning injury. Lignification, suberization, and increased sugar at the wound site have been shown to be correlated with wound healing. Changing in gene expressions have been associated with skinning injury. Genes associated in the biosynthesis of lignin and suberin, protein fate, cell-wall modification, transcription and protein synthesis, and stress responses and defense have been associated with skinning injury responses in plants. Understanding the skinning injury responses and how to regulate them can be used to produce a more desirable plant resistant to skinning injury. This paper especially reviews and discusses skinning injury responses in sweetpotato, a root crop which product may severely be affected by skinning injury. Keywords: gene expression, Ipomoea batatas, lignification, postharvest loss, wounding   ABSTRAK Pada ubi jalar (Ipomoea batatas L. Lamb), cedera kulit adalah hilangnya kulit dari permukaan umbi. Cedera kulit ini bertanggung jawab atas kerugian pascapanen yang signifikan akibat peningkatan laju kelembaban dan penurunan berat umbi, pengerutan, dan kerentanan terhadap serangan patogen. Berat umbi yang berkurang karena kehilangan air dikaitkan dengan tingkat pembusukan yang lebih tinggi, terutama terjadi di negara-negara berkembang dan yang kurang berkembang dengan kehilangan hasil panen umbi 8-20%. Tanaman memiliki adaptasi yang berbeda untuk melindungi diri dari cedera kulit. Lignifikasi, suberisasi, dan peningkatan gula di lokasi pelukaan telah terbukti berkorelasi dengan penyembuhan luka. Perubahan ekspresi gen telah dikaitkan dengan cedera kulit. Gen-gen yang terlibat dalam jalur biosintesis lignin dan suberin, protein tujuan akhir, modifikasi dinding sel, transkripsi dan sintesis protein, serta respons stres dan pertahanan telah dikaitkan dengan respons cedera kulit pada tanaman. Memahami respons cedera kulit dan bagimana cara mengaturnya dapat digunakan untuk menghasilkan tanaman yang diinginkan yang tahan terhadap cedera kulit umbi. Paper ini secara khusus mengulas dan membahas respon cedera kulit pada ubi jalar, suatu tanaman umbian yang hasilnya sangat terpengaruh oleh cedera kulit. Kata kunci: ekspresi gen, Ipomoea batatas, lignifikasi, kehilangan pascapanen, pelukaan

scholarly journals Genome-wide in silico identification and expression analysis of beta-galactosidase family members in sweetpotato [Ipomoea batatas (L.) Lam]

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Fuyun Hou ◽  
Taifeng Du ◽  
Zhen Qin ◽  
Tao Xu ◽  
Aixian Li ◽  
...  
Keyword(s):  
Plant Development ◽  
Stress Responses ◽  
Ipomoea Batatas ◽  
Expression Profiles ◽  
Glycosyl Hydrolase ◽  
Expression Patterns ◽  
Human Beings ◽  
Promoter Regions ◽  
Cell Wall Modification ◽  
Biotic Stresses

Abstract Background Sweetpotato (Ipomoea batatas (L.) Lam.) serves as an important food source for human beings. β-galactosidase (bgal) is a glycosyl hydrolase involved in cell wall modification, which plays essential roles in plant development and environmental stress adaptation. However, the function of bgal genes in sweetpotato remains unclear. Results In this study, 17 β-galactosidase genes (Ibbgal) were identified in sweetpotato, which were classified into seven subfamilies using interspecific phylogenetic and comparative analysis. The promoter regions of Ibbgals harbored several stress, hormone and light responsive cis-acting elements. Quantitative real-time PCR results displayed that Ibbgal genes had the distinct expression patterns across different tissues and varieties. Moreover, the expression profiles under various hormonal treatments, abiotic and biotic stresses were highly divergent in leaves and root. Conclusions Taken together, these findings suggested that Ibbgals might play an important role in plant development and stress responses, which provided evidences for further study of bgal function and sweetpotato breeding.

scholarly journals Effect of cutting position and vine pruning level on yield of Sweet Potato (Ipomoea Batatas L.)

1970 ◽  
pp. 01-05
Author(s):  
Ncube Netsai ◽  
Mutetwa Moses, Mtaita Tuarira
Keyword(s):  
Sweet Potato ◽  
Root Length ◽  
Ipomoea Batatas ◽  
Block Design ◽  
Root Diameter ◽  
Root Weight ◽  
Stem Cuttings ◽  
Storage Root ◽  
Storage Roots ◽  
Significant Difference

There is significant variation in yield of storage roots and vines of sweet potato (Ipomoea batatas) among farmers due to use of different cutting positions and pruning of vines at different levels. This study was carried out to establish the cutting position and the vine pruning level that give the best yield of both the storage roots and vines. The study was conducted in a 3x3 factorial arrangement in Randomized Complete Block Design (RCBD) with three replications. Treatments included cutting position at three levels (apical cutting, middle cutting and basal cutting) and pruning at three levels, 0%, 25% and 50% respectively. Pruning was done. 50 days after planting. And storage root harvesting was done 100 days after planting. The two measurements were summed up to give the total vine weight. Storage root length, diameter and weight were measured at 100 DAP. Storage root length indicated significant difference (P<0.05) only among cutting positions with highest mean length (16.20 cm) obtained from apical cutting and the lowest (11.98 cm) from basal cutting. Storage root diameter, storage root weight and vine weight indicated significant interaction (P<0.05) of cutting position and vine pruning level. Highest mean root diameter and root weight were obtained from middle cutting and 25% vine pruning level, with the lowest being obtained from basal cutting and 50% vine pruning level. Highest vine weight was recorded from middle cutting and 50% vine pruning level, with the lowest being recorded from basal cutting and 0% vine pruning level. Both middle and apical stem cuttings can be recommended for higher storage root and vine yield. Vine pruning at 25% can be adopted for higher storage root yield while pruning at 50% can be suggested for higher vine yield.

scholarly journals Genome-wide in silico identification and expression analysis of beta-galactosidase family members in sweetpotato [Ipomoea batatas (L.) Lam.]

2020 ◽  
Author(s):  
Fuyun Hou ◽  
Zhen Qin ◽  
Taifeng Du ◽  
Tao Xu ◽  
Aixian Li ◽  
...  
Keyword(s):  
Plant Development ◽  
Stress Responses ◽  
Ipomoea Batatas ◽  
Expression Profiles ◽  
Glycosyl Hydrolase ◽  
Expression Patterns ◽  
Human Beings ◽  
Promoter Regions ◽  
Cell Wall Modification ◽  
Biotic Stresses

Abstract Background: Sweetpotato (Ipomoea batatas (L.) Lam.) serves as an important food source for human beings. β-galactosidase (bgal) is a glycosyl hydrolase involved in cell wall modification, which plays essential roles in plant development and environmental stress adaptation. However, the function of bgal genes in sweetpotato remains unclear.Results: In this study, 17 β-galactosidase genes (Ibbgal) were identified in sweetpotato, which were classified into seven subfamilies using interspecific phylogenetic and comparative analyses. The promoter regions of Ibbgals harbored several stress, hormone and light responsive cis-acting elements. Quantitative real-time PCR results displayed that Ibbgal genes had the distinct expression patterns across different tissues and varieties. Moreover, the expression profiles under various hormonal treatments, abiotic and biotic stresses were highly divergent in leaves and root. Conclusions: Taken together, these findings suggested that Ibbgals might play an important role in plant development and stress responses, which provided evidences for further study of bgal function and sweetpotato breeding.

scholarly journals Genome-wide in silico identification and expression analysis of beta-galactosidase family members in sweetpotato [Ipomoea batatas (L.) Lam.]

2020 ◽  
Author(s):  
Fuyun Hou ◽  
Zhen Qin ◽  
Taifeng Du ◽  
Tao Xu ◽  
Aixian Li ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Development ◽  
Stress Responses ◽  
Ipomoea Batatas ◽  
Expression Profiles ◽  
Glycosyl Hydrolase ◽  
Expression Patterns ◽  
Human Beings ◽  
Cell Wall Polysaccharides ◽  
Cell Wall Modification

Abstract Background: Sweetpotato (Ipomoea batatas (L.) Lam.) serves as an important food source for human beings. β-galactosidase (bgal) is a glycosyl hydrolase involved in cell wall modification, which plays essential roles in plant development and environmental stress adaptation. However, the function of bgals genes in sweetpotato has yet to be reported.Results: In this study, 17 β-galactosidase genes (Ibbgal) were identified in sweetpotato, which were classified into seven subfamilies using interspecific phylogenetic and comparative analyses. The promoter regions of Ibbgals harbored several stress, hormone and light responsive cis-acting elements. Quantitative real-time PCR results displayed that Ibbgal genes had the distinct expression patterns across different tissues and varieties. Moreover, the expression profiles under various hormonal treatments, abiotic and biotic stresses were highly divergent in leaves and root. Conclusions: These findings suggest that Ibbgals may involve in plant development and stress responses through regulating the metabolism of cell wall polysaccharides.

scholarly journals Functional Classification of Skinning Injury Responsive Genes in Storage Roots of Sweetpotato

2017 ◽  
Vol 45 (1) ◽  
pp. 36-42
Author(s):  
Jollanda Effendy ◽  
Darda Efendi ◽  
Nurul Khumaida ◽  
And Gustaaf Adolf Wattimena
Keyword(s):  
Gene Expression ◽  
Stress Responses ◽  
Functional Annotation ◽  
Ipomoea Batatas ◽  
Differentially Expressed Gene ◽  
Differentially Expressed ◽  
Functional Classification ◽  
Financial Loss ◽  
Storage Roots

Skinning injury in sweetpotato due to loss of skin or periderm which occurred during harvest is inevitable and account for financial loss due to dehydration, pests, and pathogens. Hence, studies on gene expression changed due to skinning injury can provide important information about this protective tissue and for improving the life of storage roots. New candidate genes involved in skinning injury were isolated with an Annealing Control Primer (ACP). Using 20 ACP primers, a total of 103 differentially expressed genes (DEGs) were retrieved. In this study, the functional annotation of these selected 15 up-regulated DEGs (10 contigs and 5 singletons) were characterized. The results showed that these 15 “DEG-unigenes” are mainly associated with defense and stress responses, regulation and signaling, protein synthesis and fate, and metabolism may play an important role in the primary responses to skinning injury in storage roots of sweetpotato. This study showed the importance of defense and stress responses genes to the formation of wound periderm. Furthermore, this results can be used for better understanding of the molecular mechanism of skinning/mechanical injury-related genes in the storage roots of sweetpotato as well as to all stems, fruits, and roots of all plants. Keywords: differentially expressed gene, gene function, Ipomoea batatas, wounding

scholarly journals Identification and Expression of Skinning Injury-responsive Genes in Sweetpotato

2013 ◽  
Vol 138 (3) ◽  
pp. 210-216 ◽  
Author(s):  
Jollanda Effendy ◽  
Don R. La Bonte ◽  
Niranjan Baisakh
Keyword(s):  
Functional Annotation ◽  
Ipomoea Batatas ◽  
Transcript Abundance ◽  
Breeding Strategies ◽  
Storage Root ◽  
Storage Roots ◽  
Chain Reaction ◽  
Breeding Programs ◽  
Polymerase Chain ◽  
Postharvest Loss

Skinning injury in sweetpotatoes (Ipomoea batatas) is responsible for significant postharvest loss resulting from storage diseases and weight loss. Unfortunately, there is no report on the genes involved in wound healing of sweetpotato and a better understanding will facilitate improved breeding strategies. An annealing control primer (ACP) system was used to identify genes expressed after skinning injury of sweetpotato cultivar LA 07-146 storage roots. Using 20 ACPs, 63 differentially expressed genes (DEGs) were identified. Functional annotation of the DEGs revealed that genes previously shown to respond to dehydration, those involved in wounding response, and the lignin and suberin biosynthesis pathways were induced in response to skinning. Expression analysis of 18 DEGs through quantitative reverse transcription–polymerase chain reaction (PCR) showed that DEGs involved in lignin and suberin pathways were up-regulated after 8 and 12 hours of skinning. Other genes showed up- or down-regulation in their transcript abundance depending on the time the storage root was sampled after intentional skinning. The genes up-regulated in response to skinning may be useful to identify expression markers for screening sweetpotato lines tolerant to skinning injury in breeding programs.

scholarly journals Genome-wide identification and expression analysis of beta-galactosidase family members in sweetpotato [ Ipomoea batatas (L.) Lam.]

2020 ◽  
Author(s):  
zongyun li ◽  
fuyun hou ◽  
zhen qin ◽  
Taifeng Du ◽  
Tao Xu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Development ◽  
Stress Responses ◽  
Ipomoea Batatas ◽  
Glycosyl Hydrolase ◽  
Human Beings ◽  
Cell Wall Polysaccharides ◽  
Promoter Regions ◽  
Cell Wall Modification ◽  
Biotic Stresses

Abstract Background: Sweetpotato (Ipomoea batatas (L.) Lam.) serves as an important food source for human beings. β-galactosidase (β-gal) is a glycosyl hydrolase involved in cell wall modification, which plays essential roles in plant development and environmental stress adaptation. However, the function of β-gals genes in sweetpotato has yet to be reported.Results: In this study, 17 β-galactosidase genes (Ibbgal) were identified in sweetpotato, which were classified into seven subfamilies using interspecific phylogenetic and comparative analyses. The promoter regions of Ibbgals harbored several stress, hormone and light responsive cis-acting elements. The Ibbgal genes were specifically expressed in different tissues and varieties, and differentially expressed under various hormonal treatments, and abiotic and biotic stresses.Conclusions: These findings suggest that Ibbgals may involve in plant development and stress responses through regulating the metabolism of cell wall polysaccharides.

scholarly journals Transcriptomic and Metabolic Profiling of High-Temperature Treated Storage Roots Reveals the Mechanism of Saccharification in Sweetpotato (Ipomoea batatas (L.) Lam.)

2021 ◽  
Vol 22 (13) ◽  
pp. 6641
Author(s):  
Chen Li ◽  
Meng Kou ◽  
Mohamed Hamed Arisha ◽  
Wei Tang ◽  
Meng Ma ◽  
...  
Keyword(s):  
High Temperature ◽  
Metabolic Pathways ◽  
Transcriptome Sequencing ◽  
Ipomoea Batatas ◽  
Sequencing Analysis ◽  
Storage Roots ◽  
Metabolic Analysis ◽  
Cooking Process

The saccharification of sweetpotato storage roots is a common phenomenon in the cooking process, which determines the edible quality of table use sweetpotato. In the present study, two high saccharified sweetpotato cultivars (Y25, Z13) and one low saccharified cultivar (X27) in two growth periods (S1, S2) were selected as materials to reveal the molecular mechanism of sweetpotato saccharification treated at high temperature by transcriptome sequencing and non-targeted metabolome determination. The results showed that the comprehensive taste score, sweetness, maltose content and starch change of X27 after steaming were significantly lower than those of Y25 and Z13. Through transcriptome sequencing analysis, 1918 and 1520 differentially expressed genes were obtained in the two periods of S1 and S2, respectively. Some saccharification-related transcription factors including MYB families, WRKY families, bHLH families and inhibitors were screened. Metabolic analysis showed that 162 differentially abundant metabolites related to carbohydrate metabolism were significantly enriched in starch and sucrose capitalization pathways. The correlation analysis between transcriptome and metabolome confirmed that the starch and sucrose metabolic pathways were significantly co-annotated, indicating that it is a vitally important metabolic pathway in the process of sweetpotato saccharification. The data obtained in this study can provide valuable resources for follow-up research on sweetpotato saccharification and will provide new insights and theoretical basis for table use sweetpotato breeding in the future.

scholarly journals Overexpression of the Golden SNP-Carrying Orange Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants

Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 51
Author(s):  
So-Eun Kim ◽  
Chan-Ju Lee ◽  
Sul-U Park ◽  
Ye-Hoon Lim ◽  
Woo Sung Park ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Tolerance ◽  
Ipomoea Batatas ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cauliflower Mosaic Virus ◽  
Carotenoid Content ◽  
35S Promoter ◽  
Storage Roots ◽  
Heat Stress Tolerance

Carotenoids function as photosynthetic accessory pigments, antioxidants, and vitamin A precursors. We recently showed that transgenic sweetpotato calli overexpressing the mutant sweetpotato (Ipomoea batatas [L.] Lam) Orange gene (IbOr-R96H), which carries a single nucleotide polymorphism responsible for Arg to His substitution at amino acid position 96, exhibited dramatically higher carotenoid content and abiotic stress tolerance than calli overexpressing the wild-type IbOr gene (IbOr-WT). In this study, we generated transgenic sweetpotato plants overexpressing IbOr-R96H under the control of the cauliflower mosaic virus (CaMV) 35S promoter via Agrobacterium-mediated transformation. The total carotenoid contents of IbOr-R96H storage roots (light-orange flesh) and IbOr-WT storage roots (light-yellow flesh) were 5.4–19.6 and 3.2-fold higher, respectively, than those of non-transgenic (NT) storage roots (white flesh). The β-carotene content of IbOr-R96H storage roots was up to 186.2-fold higher than that of NT storage roots. In addition, IbOr-R96H plants showed greater tolerance to heat stress (47 °C) than NT and IbOr-WT plants, possibly because of higher DPPH radical scavenging activity and ABA contents. These results indicate that IbOr-R96H is a promising strategy for developing new sweetpotato cultivars with improved carotenoid contents and heat stress tolerance.

scholarly journals Modeling the impact of sweetpotato weevils on storage root yield

2018 ◽  
Vol 3 (1) ◽  
pp. 319-325
Author(s):  
Daniel A. Akansake ◽  
Putri E. Abidin ◽  
E. E. Carey
Keyword(s):  
Ipomoea Batatas ◽  
Soft Rot ◽  
Northern Ghana ◽  
Root Yield ◽  
Storage Roots ◽  
Yield Data ◽  
Level Of Significance ◽  
Attainable Yield ◽  
The Impact ◽  
Model Approach

Abstract This study estimated the amount of loss in storage roots caused by various levels of damage caused by sweetpotato weevils (Cylas spp). Seven varieties of sweetpotato (Ipomoea batatas L. (Lam)) were evaluated in three production sites in northern Ghana for two years (2014 and 2015). Yield data for each experimental plot were collected. A regression analysis was carried out using the generalized linear model approach. In the study, nonmarketable roots were classified as all undersized roots (<100g) and spoilt roots due to weevil, millipede, and soft rot. The results indicated weevil damage as the only significant predictor of nonmarketable yield at 5% level of significance. From the study, the average values for total root yield, marketable root yield, and nonmarketable root yield were 9.39, 6.71, and 2.67 ton/ha respectively. The minimum weevil damage (score 2) resulted in a yield loss of 2 ton/ha which represents 8.3% while severe damage at score 9 could cause a loss of 7.43 ton/ha of storage roots representing 31% of the attainable yield of sweetpotato. Weevil susceptibility needs to be treated as a serious trait when evaluating sweetpotato genotypes to be released as varieties.

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