scholarly journals Virgin Coconut Oil (VCO) Accelerated Wound Healing Process in Diabetes mellitus (DM) Patients With Diabetic Ulcer in dr. Rasidin Hospital, Padang, Indonesia

2020 ◽  
Vol 5 (2) ◽  
pp. 221-224
Author(s):  
Putri Dafriani ◽  
Siti Aisyah Nur ◽  
Honesty Diana Morika ◽  
Roza Marlinda
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Healing Process ◽  
Coconut Oil ◽  
Wound Healing Process ◽  
Virgin Coconut Oil ◽  
Diabetic Ulcer

scholarly journals Topical ozonated virgin coconut oil improves wound healing and increases HSP90α, VEGF-A, EGF, bFGF, and CD34 in diabetic ulcer mouse model of wound healing

F1000Research ◽  
2021 ◽  
Vol 9 ◽  
pp. 580
Author(s):  
Renni Yuniati ◽  
Prasetyowati Subchan ◽  
Wibi Riawan ◽  
Matthew Brian Khrisna ◽  
Maryam Restiwijaya ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Model ◽  
Healing Process ◽  
Coconut Oil ◽  
Control Group ◽  
Wound Healing Process ◽  
Virgin Coconut Oil ◽  
Diabetic Ulcer ◽  
Macroscopic Appearance ◽  
Wound Length

Background: Diabetes is a disease that affects people worldwide, including in Indonesia. The prevalence of diabetes in Indonesia is increasing from year to year. One of the most devastating complications of diabetes mellitus is diabetic ulcers, which is a limb-threatening complication. Over the past few decades, ozone generated using plasma medical technology has been investigated as an agent that helps wound healing. This study aims to evaluate the effects of topical ozonated virgin coconut oil (VCO) in a diabetic wound mouse model. Methods: This study was an experimental study with a post-test control design. An ulcer wound model was made in 50 diabetic male Wistar mice, divided into five groups, and a control group of 10 non-diabetic mice. The control groups were given conventional therapy only and the treatment groups were also given topical ozonated VCO with different flow durations (0 min, 90 min, 7 h, 14 h). Macroscopic appearance and wound contraction were observed. HSP90β, VEGF-A, EGF, bFGF, and CD34 levels were measured from the immunostained slices of wound margins. Results: The reduction of wound length was proportionally related to the duration of ozone flow. Ozonated VCO with a longer duration of ozone flow healed the wound more quickly and had the shortest wound length. VCO with ozone flow for 14 hours (16837.10 µm) had the biggest reduction in wound length compared to other groups. The wounds treated with ozonated VCO showed an increase in HSP90β, VEGF-A, EGF, bFGF, and CD34 levels that correlated to improved wound healing. A longer period of treatment resulted in higher levels of wound healing biomarkers compared to shorter therapeutic durations. Conclusions: Topical ozonated VCO improved the wound healing process in a diabetic ulcer mouse model by improving macroscopic wound appearance and increasing levels of wound healing biomarkers.

scholarly journals Topical ozonated virgin coconut oil improves wound healing and increases HSP90α, VEGF-A, EGF, bFGF and CD34 in diabetic ulcer mouse model of wound healing

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 580
Author(s):  
Renni Yuniati ◽  
Prasetyowati Subchan ◽  
Wibi Riawan ◽  
Matthew Brian Khrisna ◽  
Maryam Restiwijaya ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Model ◽  
Healing Process ◽  
Coconut Oil ◽  
Control Group ◽  
Wound Healing Process ◽  
Virgin Coconut Oil ◽  
Diabetic Ulcer ◽  
Macroscopic Appearance ◽  
Wound Length

Background: Diabetes is a disease that affects people worldwide, including in Indonesia. The prevalence of diabetes in Indonesia is increasing from year to year. One of the most devastating complications of diabetes mellitus is diabetic ulcers, which is a limb-threatening complication. Over the past few decades, ozone generated using plasma medical technology has been investigated as an agent that helps wound healing. This study aims to evaluate the effects of topical ozonated virgin coconut oil (VCO) in a diabetic wound mouse model. Methods: This study was an experimental study with a post-test control design. An ulcer wound model was made in 50 diabetic male Wistar mice, divided into five groups, and a control group of 10 non-diabetic mice. The control groups were given conventional therapy only and the treatment groups were also given topical ozonated VCO with different flow durations (0 min, 90 min, 7 h, 14 h). Macroscopic appearance and wound contraction were observed. HSP90β, VEGF-A, EGF, bFGF and CD34 levels were measured from the immunostained slices of wound margins. Results: The reduction of wound length was proportionally related to the duration of ozone flow. Ozonated VCO with a longer duration of ozone flow healed the wound more quickly and had the shortest wound length. VCO with ozone flow for 14 hours (16837.10 µm) had the biggest reduction in wound length compared to other groups. The wounds treated with ozonated VCO showed an increase in HSP90β, VEGF-A, EGF, bFGF and CD34 levels that correlated to improved wound healing. A longer period of treatment resulted in higher levels of wound healing biomarkers compared to shorter therapeutic durations. Conclusions: Topical ozonated VCO improved the wound healing process in a diabetic ulcer mouse model by improving macroscopic wound appearance and increasing levels of wound healing biomarkers.

scholarly journals The Activity of Hydrolyzed Virgin Coconut Oil to Increase Proliferation and Cyclooxygenase-2 Expression towards on NIH 3T3 Cell Line in Wound Healing Process

2019 ◽  
Vol 7 (19) ◽  
pp. 3164-3168
Author(s):  
Jansen Silalahi ◽  
Yuandani Yuandani ◽  
Dian Ika Perbina Br Meliala ◽  
Linda Margata ◽  
Denny Satria
Keyword(s):  
Fatty Acids ◽  
Cell Proliferation ◽  
Wound Healing ◽  
Healing Process ◽  
Coconut Oil ◽  
Acid Value ◽  
Wound Healing Process ◽  
Virgin Coconut Oil ◽  
Cox 2 ◽  
Nih 3T3

AIM: This study aims to determine the effect of hydrolysed virgin coconut oil (HVCO) to increase cell proliferation, COX-2 expression of NIH 3T3. METHODS: The sample used was Virgin Coconut Oil (VCO). VCO was partially hydrolysed using lipase from Rhizomucor miehei (active on sn-1,3 position) to produce hydrolysed VCO (HVCO) composed of free fatty acids, 2-monoglycerides. Then acid value was determined. The effect of HVCO on proliferation was evaluated using the MTT method. Wound healing assay was established by a cell migration method, and COX-2 expression was determined using RT-PCR. RESULTS: Acid value is 135.89 ± 0.12 mg NaOH/g oil and free fatty acids (FFA) is 48.50 ± 0.06%. The effect of HVCO 62.5 µg/mL on cell proliferation after 24h, 48h, and 72h incubation found as viable cells are 109.24 ± 0.52%; 118.26 ± 0.91% and 106.59 ± 0.74%. Percent of wound closed after 24 h and 48 h incubation are 69.94 ± 0.54% and 100.00 ± 0.00%, and expression of COX-2 increased from 1 (control) to 1.83 (HVCO). CONCLUSION: The results suggest that HVCO is effective to increase cells proliferation and hence wound healing process.

scholarly journals Effectiveness Wound Care Using Modern Dressing Method To Diabetic Wound Healing Process Of Patient With Diabetes Mellitus

2017 ◽  
Vol 1 (2) ◽  
pp. 172-181
Author(s):  
Windu Santoso ◽  
Joyo Purnomo
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Wound Care ◽  
Healing Process ◽  
T Test ◽  
Wound Healing Process ◽  
Dressing Method ◽  
Diabetic Ulcer ◽  
Significance Level ◽  
Paired T Test

Diabetes mellitus causes a lot of various complaints and complications. One of the most serious and most common complications is diabetic ulcers. Prevention of diabetic ulcer complications can be done with comprehensive wound care. The Appropriate wound care management can prevent amputation. One of a method of wound care that can accelerate the wound healing process is modern dressing wounds. This researched aim to analyze the effectiveness of wound care using modern dressing method to the diabetes mellitus patients. The research design was pre-experimental one group pre-test post-test with 15 samples.  The data analyzed using Paired T-test. The result of data analysis using Paired T-test with significance level of 5% obtained ρ = 0,000 and the average value of wound development before and after wound treatment using modern dressing method has decrease from 39,67to  29,93 because wound care with modern dressing method make environment of wound is moist so that capitalization and granulation process growth up be faster. So it can be concluded that there is an effect of wound care using modern dressing method to the wound healing process with diabetes mellitus.

scholarly journals Effectiveness Wound Care Using Modern Dressing Method To Diabetic Wound Healing Process Of Patient With Diabetes Mellitus

2017 ◽  
Vol 1 (2) ◽  
pp. 172-181
Author(s):  
Windu Santoso ◽  
Joyo Purnomo
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Wound Care ◽  
Healing Process ◽  
T Test ◽  
Wound Healing Process ◽  
Dressing Method ◽  
Diabetic Ulcer ◽  
Significance Level ◽  
Paired T Test

Diabetes mellitus causes a lot of various complaints and complications. One of the most serious and most common complications is diabetic ulcers. Prevention of diabetic ulcer complications can be done with comprehensive wound care. The Appropriate wound care management can prevent amputation. One of a method of wound care that can accelerate the wound healing process is modern dressing wounds. This researched aim to analyze the effectiveness of wound care using modern dressing method to the diabetes mellitus patients. The research design was pre-experimental one group pre-test post-test with 15 samples.  The data analyzed using Paired T-test. The result of data analysis using Paired T-test with significance level of 5% obtained ρ = 0,000 and the average value of wound development before and after wound treatment using modern dressing method has decrease from 39,67to  29,93 because wound care with modern dressing method make environment of wound is moist so that capitalization and granulation process growth up be faster. So it can be concluded that there is an effect of wound care using modern dressing method to the wound healing process with diabetes mellitus.

scholarly journals PENGARUH APLIKASI VIRGIN COCONUT OIL TERHADAP PENINGKATAN JUMLAH FIBROBLAS PADA LUKA PASCA PENCABUTAN GIGI PADA RATTUS NOVERGICUS

2015 ◽  
Vol 1 (2) ◽  
pp. 29
Author(s):  
Anggun Hibah Jannah Tamara ◽  
Yayun Siti Rochmah ◽  
Rochman Mujayanto
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Health Benefit ◽  
Coconut Oil ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Complex Biological Process ◽  
Significant Difference ◽  
Per Oral ◽  
Levene Test

Background: Wound healing is a complex biological process. Fibroblast is one of cell which is responsible for the wound healing process. Virgin Coconut Oil (VCO) have health benefit as antibacterial, antiinflamatory, antioxidant, and immunomodulatory. This research was purposed to know the influence use of VCO over the amount of fibroblast in wound healing after dental extraction.Method: Research quasi experimental with post test control group design and using 15 Rattus novergicus which were divided into three groups (control positive, VCO topical application, and VCO per oral application). Tooth removal was committed by using explorer, scalpel-blade, and arterial clamp. Then VCO applied everyday for 7 days. Day 7th Rattus novergicus were died, the socket after extraction were made into histological form, then the amount of fibroblast were counted with 5 field of views and made in average. Result: The number of fibroblast of the most numerous was VCO per oral ( 48,6 ± 49,8 ), VCO topical ( 36,2 ± 38,8 ), and control positive ( 33,4 ± 35,2 ). The results of the Saphiro Wilk test and Levene test acquired data normally distributed and homogeny, so it continued to One Way Anova and data showed there were significant difference among the groups of samples with value p<0,05.Conclusion: The conclusion of this research is VCO could increase the number of fibroblasts in wound healing after dental extractions

scholarly journals The Role of Coconut Oil to Increase Expression of MMP-9, PDGF-BB, and TGF-β1 in NIH-3T3 Cell Line

2019 ◽  
Vol 7 (22) ◽  
pp. 3733-3736
Author(s):  
Dian Ika Perbina Meliala ◽  
Jansen Silalahi ◽  
Yuandani Yuandani ◽  
Linda Margata ◽  
Denny Satria
Keyword(s):  
Healing Process ◽  
Coconut Oil ◽  
Wound Healing Process ◽  
3T3 Cells ◽  
Virgin Coconut Oil ◽  
Nih3t3 Cells ◽  
Nih 3T3 ◽  
Tgf Β1 ◽  
Nih 3T3 Cells

AIM: The objective of the study was to evaluate protein expression in NIH 3T3 cells that are treated with virgin coconut oil (VCO) and hydrolysed of virgin coconut oil (HVCO) in vitro. METHODS: Coconut oil used in this study was virgin coconut oil (VCO) and VCO hydrolysed by Rhizomucor miehei (HVCO). NIH 3T3 cells (5x105 cells/well) were seeded in nine wells and incubated for overnight, then divided into three groups. Each group consisted of three wells. Group one without treatment, group two added VCO, and group three added HVCO and then incubated for overnight. One well in each group was added MMP-9, PDGF-BB, and TGF-β1 and incubated one hour. Finally, expressions of MMP-9, PDGF-BB, and TGF-β1 were detected using immunocytochemistry method. RESULTS: The results of the study showed that VCO and HVCO increased protein expressions of MMP-9, PDGF-BB, and TGF-β1. Percentage of MMP-9 expressions treated by VCO increased from 2.89 ± 0.07 to 28.16 ± 0.34, PDGF-BB from 28.11 ± 0.13 to 48.53 ± 0.49, and TGF-β1 from 4.19 ± 0.08 to 18.41 ± 0.54. Percentage of MMP-9 expressions treated by HVCO increased from 2.89 ± 0.07 to 55.40 ± 0.94, PDGF-BB from 28.11 ± 0.13 to 61.65 ± 0.42, and TGF-β1 from 4.19 ± 0.08 to 36.35 ± 0.67. CONCLUSION: VCO and HVCO increase the expression of MMP-9, PDGF-BB, dan TGF-β1 in NIH3T3 cells and therefore, coconut oil active in the wound healing process. HVCO is more than active than VCO.

scholarly journals PROSES PENYEMBUHAN LUKA ULKUS DIABETIKUM DENGAN METODE MODERN DRESSING DIKLINIK MAITIS EFRANS WOUND CARE

2018 ◽  
Vol 10 (2) ◽  
pp. 146-151
Author(s):  
Nadya Putri Nabila
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Wound Care ◽  
Healing Process ◽  
Case Study Research ◽  
World Population ◽  
Care Clinic ◽  
Diabetic Ulcer ◽  
Patients With Diabetes

Diabetes mellitus (DM) is one of the most common chronic diseases experienced by the world population and ranks fourth cause of death in developing countries. Long-term complications of diabetes mellitus one of them is diabetic ulcer (15%) and is the most cause (85%) of amputation in patients with diabetes mellitus. Currently, more than 5,000 modern types of dressings are reported to be available to treat wounds, especially diabetic ulcers. To know the process of wound healing diabetic ulcer was done with the design of case study research with a sample of 2 people and this study was conducted for 4 weeks. The study was conducted at the Maitis Efrans Wound Care clinic in Bengkulu City. The result was obtained that the assessment of diabetic ulcer wounds before modern wound care on the respondents was a total score of 54 and the respondents two total score of 50 were stated wound regeneration. The healing process of the responder's second ulcers progressed, the total score of one respondent was 30 and the respondent two was 28. Respondents. Progress on the two respondents stated better influenced by wound healing factor that is, age factor.

scholarly journals Effect of Red Fruit Oil Soap (Pandanus Conoideus Lam.) as Wound Cleansing on Wound Healing and the Number of Bacterial Colonies among Grade II Diabetic Ulcer Patients at Griya Wound Care Clinic Kudus, Indonesia

2019 ◽  
Vol 3 (2) ◽  
pp. 55
Author(s):  
Hevny Kartika Dewi ◽  
Mardiyono Mardiyono ◽  
Diyah Fatmasari ◽  
Sudirman Sudirman ◽  
Djenta Saha
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Intervention Group ◽  
Control Group ◽  
Wound Healing Process ◽  
Nacl Solution ◽  
Diabetic Ulcer ◽  
Grade Ii ◽  
The Mean ◽  
Fruit Oil

Background: Diabetic ulcer is an open wound on the skin layer to the dermis due to hyperglycemia and neuropathy. This condition often causes infection and becomes an inhibitor in the wound healing process. Red fruit oil soap can be used as an alternative cleansing to reduce the number of bacterial colonies and accelerate the wound healing process.Aims: This present study aimed to observe the effect of red fruit oil soap in the cleansing process to reduce the number of bacterial colonies and the wound healing process in grade II Diabetic ulcer patientsMethods: This is a quasi-experimental study with the pre-test post-test non-equivalent control group design. Wound cleansing in the intervention group used 0.9% NaCl solution and red fruit oil soap with a pH of 5.74, while the wound cleansing in the control group used 0.9% NaCl solution. Bacterial colonies were assessed and the type of bacteria was observed by the swab method. The wound healing was assessed with Bates-Jensen Wound Assessment Tool (BWAT) instrument for 14 days with observations every 3 days. Analysis test used Mann Whitney and Repeated Measure ANOVA.Results: There was no difference in the mean of the number of bacterial colonies between the wounds that were cleansed with 0.9% NaCl solution and red fruit oil soap and the wounds which were cleansed by using 0.9% NaCl solution (p> 0.05). The mean of the number of colonies in the intervention group until the 14th day reduced by 3.14x106 and in the control group was reduced h 1.40x106. There was a significant decrease in the wound healing scores in each group, in each assessment for 14 days.Conclusion: This study found that the wound cleansing using red fruit oil soap and 0.9% NaCl solution could reduce the number of bacterial colonies on the wound surface and accelerate the wound healing process among patients with grade II diabetic ulcer.

scholarly journals Redox treatment ameliorates diabetes mellitus-induced skin flap necrosis via inhibiting apoptosis and promoting neoangiogenesis

2020 ◽  
pp. 153537022097426
Author(s):  
Yeon S Kim ◽  
Hye-Young Lee ◽  
Jeon Y Jang ◽  
Hye R Lee ◽  
Yoo S Shin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Wound Healing ◽  
Animal Model ◽  
Cell Viability ◽  
Skin Flap ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Wound Healing Process ◽  
In Vivo Animal Model

Intractable wound healing is the habitual problem of diabetes mellitus. High blood glucose limits wound healing by interrupting inflammatory responses and inhibiting neoangiogenesis. Oxidative stress is commonly thought to be a major pathogenic cause of diabetic complications. Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one, EDV) is a free radical scavenger which suppress oxidative stress. This study investigates whether EDV can reduce oxidative stress in wound healing HaCaT/human dermal fibroblasts cells (HDFs) in vitro and in vivo animal model. Cell viability and wound healing assays, FACS flow cytometry, and Hoechst 33342 staining were performed to confirm apoptosis and cytotoxicity in H2O2 and EDV-treated HaCaT and HDFs. A streptozotocin-induced hyperglycemic animal model was made in adult C57BL6 mice. Full-thickness skin flap was made on dorsomedial back and re-sutured to evaluate the wound healing process. EDV was delivered slowly in the skin flap with degradable fibrin glue. The flap was monitored and analyzed on postoperative days 1, 3, and 5. CD31/DAPI staining was done to detect newly formed blood vessels. The expression levels of NF-κB, bcl-2, NOX3, and STAT3 proteins in C57BL6 mouse tissues were also examined. The wound healing process in hyper- and normoglycemic mice showed a difference in protein expression, especially in oxidative stress management and angiogenesis. Exogenous H2O2 reduced cell viability in a proportion to the concentration via apoptosis. EDV protected HaCaT cells and HDFs from H2O2 induced reactive oxygen species cell damage and apoptosis. In the mouse model, EDV with fibrin resulted in less necrotic areas and increased angiogenesis on postoperative day 5, compared to sham-treated mice. Our results indicate that EDV could protect H2O2-induced cellular injury via inhibiting early apoptosis and inflammation and also increasing angiogenesis. EDV might be valuable in the treatment of diabetic wounds that oxidative stress has been implicated.

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