Phytochemical study and antimicrobial activities of extracts and their derived fractions obtained from Berberis vulgaris L. and Stellaria media L. leaves

Marrubium vulgare is used worldwide as a source of food flavor and for medicinal purposes. The aim of this study is to investigate polyphenol and flavonoid contents of M. vulgare extracts and their antimicrobial activities. Extraction was conducted using methanol and hexane. The determination of polyphenol content was realized with folin ciocalteu method and flavonoids using AlCl3. Rouph characterization of these compounds was done with HPLC method. Activity against bacteria and fungi was also studied. Results showed that methanolic extracts of leaves (LME) and stems SME) contain relatively high levels of polyphenols ad flavonoids. Except for hexane extract, all extracts from leaves and stems possess antibacterial and antifungal effects especially against Staphyloccocus aureus and Candida albicans. This finding suggests that M. vulgare methanolic extracts could serve as a basic material for the preparation of antimicrobial drugs. Keywords: Marrubium vulgare, polyphenols, flavonoids, antibacterial, antifungal activities.

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New Alkenylresorcinols with Cytotoxic and Antimicrobial Activities from the Leaves of Embelia schimperi

Planta Medica ◽

10.1055/a-1209-3501 ◽

2020 ◽

Vol 86 (17) ◽

pp. 1298-1303

Author(s):

Tatiana Arlette M. Togue ◽

Blanche Laure Ndontsa ◽

Gabin Thierry M. Bitchagno ◽

Anja Schüffler ◽

Till Opatz ◽

...

Keyword(s):

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Spectroscopic Techniques ◽

Ongoing Research ◽

Phytochemical Study ◽

Phenolic Lipids ◽

Cervical Cancer Cells ◽

Hela S3 ◽

Human Cervical Cancer Cells

AbstractA phytochemical study of the methanol extract of the leaves of Embelia schimperi resulted in the isolation of three new alkenylresorcinols, 1 – 3, together with the known analogs 4 – 7. Their structures were established by a combination of spectroscopic techniques. Compounds 1 – 7 exhibited moderate cytotoxic activity against human cervical cancer cells HeLa-S3 and more pronounced antimicrobial properties towards bacteria and filamentous fungi. The present study falls into an ongoing research project on the characterization of bioactive phenolic lipids from plants of the family Primulaceae.

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PHYTOCHEMICAL STUDY AND ANTIMICROBIAL ACTIVITIES OF CORDIA DICHOTOMA

International Research Journal of Pharmacy ◽

10.7897/2230-8407.041212 ◽

2014 ◽

Vol 4 (12) ◽

pp. 53-56 ◽

Cited By ~ 3

Author(s):

Suman Rawat ◽

Ruchi Saini ◽

Ankita Sharma

Keyword(s):

Antimicrobial Activities ◽

Phytochemical Study

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Bioactive Characteristics of Wild Berberis vulgaris and Berberis crataegina Fruits

Journal of Chemistry ◽

10.1155/2020/8908301 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Arzu Yaşar Eroğlu ◽

Özlem Çakır ◽

Mustafa Sağdıç ◽

Enes Dertli

Keyword(s):

Antioxidant Capacity ◽

Pathogenic Bacteria ◽

Phenolic Content ◽

Antimicrobial Activities ◽

Physicochemical Analysis ◽

Matter Content ◽

Total Phenolic ◽

Dry Matter Content ◽

Berberis Vulgaris ◽

Wild Fruits

There is an increasing trend to find novel sources of products with high antioxidant capacity and wild fruits are very good examples for these sources. In this study, fruits of Berberis vulgaris and Berberis crataegina, naturally grown in Bayburt province of Turkey, were tested for their physicochemical features, antioxidant capacities, phenolic compound profiles, and antimicrobial activities. The physicochemical analysis of the fruits revealed that the dry matter content, ash content, pH, and aw values were between 28.47% and 41.61%, 0.65% and 2.13%, 2.44 and 3.25, and 0.996 and 0.97, respectively. The total phenolic content of the fruits was determined by the Folin–Ciocalteu methodology, and for the determination of the antioxidant capacity of the fruits, DPPH, ABTS, and β-carotene bleaching methods were performed and a high level of antioxidant activity was observed. HPLC analysis was applied to identify the phenolic content of the fruits, and gallic acid and chlorogenic acid were found to be the dominant phenolic compounds in Berberis fruits. The water extracts of the Berberis fruits were tested against important foodborne pathogenic bacteria as potential antimicrobials. The extracts inhibited the growth of Bacillus cereus, Salmonella typhimurium, Yersinia enterocolitica, and Staphylococcus aureus at significant rates. This study revealed the potential antioxidant and antimicrobial characteristics of wild-type B. vulgaris and B. crataegina that can be used for different future applications.

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Phytochemistry of Verbascum Species Growing in Iraqi Kurdistan and Bioactive Iridoids from the Flowers of Verbascum calvum

Plants ◽

10.3390/plants9091066 ◽

2020 ◽

Vol 9 (9) ◽

pp. 1066

Author(s):

Hawraz Ibrahim M. Amin ◽

Faiq H. S. Hussain ◽

Gianluca Gilardoni ◽

Zaw Min Thu ◽

Marco Clericuzio ◽

...

Keyword(s):

Traditional Medicine ◽

Methanol Extract ◽

Skin Diseases ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Traditional Use ◽

Phytochemical Study ◽

Mountainous Regions ◽

Iraqi Kurdistan ◽

Iridoid Glucosides

Traditional medicine is still widely practiced in Iraqi Kurdistan, especially by people living in villages on mountainous regions; medicinal plants are also sold in the markets of the large towns, such as at Erbil, the capital of the Kurdistan Autonomous Region. About a dozen of Verbascum species (Scrophulariaceae) are commonly employed in the Kurdish traditional medicine, especially for treating burns and other skin diseases. However, the isolation of bioactive secondary metabolites from these plants has not been the subject of intense scientific investigations in Iraq. Therefore, the information reported in the literature about the species growing in Kurdistan has been summarized in the first part of this paper, although investigations have been performed on vegetable samples collected in neighbouring countries, such as Turkey and Iran. In the second part of the work, we have investigated, for the first time, the contents of a methanol and a hydromethanol extract of V. calvum flowers. The extracts exhibited weak antimicrobial activities, whereas the methanol extract showed significant antiproliferative effects against an A549 lung cancer cell line. Moreover, both extracts exhibited a significant dose-dependent free radical scavenging action against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, comparable to that of ascorbic acid. In the subsequent phytochemical study, a high phenolic content was determined in both extracts by the Folin–Ciocalteu assay and medium-pressure liquid chromatographic (MPLC) separation led to the isolation of iridoid glucosides ajugol and aucubin from the methanol extract. In conclusion, the high anti-inflammatory effects of aucubin and the remarkable antioxidant (antiradical) properties of the extracts give scientific support to the traditional use of V. calvum flowers for the preparation in Kurdistan of remedies to cure skin burns and inflammations.

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PHYTOCHEMICAL STUDY, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF EUPHORBIA RESINIFERA L.

International Research Journal of Pharmacy ◽

10.7897/2230-8407.04910 ◽

2013 ◽

Vol 2 (9) ◽

pp. 44-50

Author(s):

Houcine Benmehdi ◽

Nadia Bounoua ◽

Abdelillah Amrouche ◽

Driss Lahcene ◽

Abdelhak Maazouzi

Keyword(s):

Antimicrobial Activities ◽

Phytochemical Study ◽

Antioxidant And Antimicrobial Activities

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Antileishmanial, Antimalarial and Antimicrobial Activities of the Extract and Isolated Compounds from Austroplenckia populnea (Celastraceae)

Zeitschrift für Naturforschung C ◽

10.1515/znc-2008-7-805 ◽

2008 ◽

Vol 63 (7-8) ◽

pp. 497-502 ◽

Cited By ~ 10

Author(s):

Sérgio F. Andrade ◽

Ademar A. da Silva Filho ◽

Wilson R. Cunha ◽

N. P. Dhammika Nanayakkara ◽

Jairo Kenupp Bastos

Keyword(s):

Mammalian Cells ◽

Leishmania Donovani ◽

Folk Medicine ◽

Vero Cells ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Phytochemical Study ◽

Hydroalcoholic Extract ◽

Cytotoxicity Activity ◽

First Time

Austroplenckia populnea (Celastraceae), known as “marmelinho do campo”, is used in Brazilian folk medicine as antimicrobial, anti-inflammatory, and antitumoural agent. The aim of the present work was to evaluate the antimicrobial, antileishmanial and antimalarial activities of the crude hydroalcoholic extract of A. populnea (CHE) and some of its isolated compounds. The phytochemical study of the CHE was carried out affording the isolation of methyl populnoate (1), populnoic acid (2), and stigmast-5-en-3-O-β-(d-glucopyranoside) (3). This is the first time that the presence of compound 3 in A. populnea is reported. The results showed that the CHE presents antifungal and antibacterial activities, especially against Candida glabrata and Candida albicans, for which the CHE showed IC50 values of 0.7 μg mL-1 and 5.5 μg mL-1, respectively, while amphotericin B showed an IC50 value of 0.1 μg mL-1 against both microorganisms. Compounds 1D3 were inactive against all tested microorganisms. In the antileishmanial activity test against Leishmania donovani, the CHE showed an IC50 value of 52 μg mL-1, while compounds 2 and 3 displayed an IC50 value of 18 μg mL-1. In the antimalarial assay against Plasmodium falciparum (D6 and W2 clones), it was observed that all evaluated samples were inactive. In order to compare the effect on the parasites with the toxicity to mammalian cells, the cytotoxicity activity of the isolated compounds was evaluated against Vero cells, showing that all evaluated samples exhibited no cytotoxicity at the maximum dose tested.

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Phytochemical Study on the Essential Oils of Tarragon (Аrtemisia dracunculus L.) Growing in Tajikistan and Its Comparison With the Essential Oil of the Species in the Rest of the World

Natural Product Communications ◽

10.1177/1934578x20977394 ◽

2020 ◽

Vol 15 (12) ◽

pp. 1934578X2097739

Author(s):

Farukh S. Sharopov ◽

Aminjon Salimov ◽

Sodik Numonov ◽

Mahinur Bakri ◽

Zafar Sangov ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Hierarchical Cluster ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Methyl Eugenol ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Study ◽

Aerial Parts ◽

Total Oil

The aerial parts of the tarragon ( Artemisia dracunculus) were collected around Kukteppa village, Ziddi, Varzob region of Tajikistan. The essential oil of tarragon was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. Forty-five compounds representing 99.8% of total oil were identified. Sabinene (29.1%), estragole (24.6%), limonene (7.8%), ( Z)-artemidin (4.9%), myrcene (4.8%), and ( E)-β-ocimene (4.0%) were components with a representation higher than 4% of the essential oils of aerial parts of tarragon. Hierarchical cluster analysis of А. dracunculus essential oils on the global phytogeographic origin based on 30 essential oil components and 105 samples (globally) of this species, indicated the existence of its 7 major chemotypes: ocimene, α-terpinene, capillene, methyl eugenol, mixed chemotype, ( Z)-artemidin, and estragol chemotypes. The essential oils of А. dracunculus showed weak antioxidant and antibacterial activities. To our best knowledge, this is the first report concerning the chemical composition, chemotypic variation, antioxidant, and antimicrobial activities of the essential oils obtained from the aerial parts of А. dracunculus, growing wild in the Varzob region of Tajikistan.

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A New Lignan, Phytochemical Study and Antimicrobial Activities of Zanthoxylum monophyllum

Planta Medica ◽

10.1055/s-0031-1273574 ◽

2011 ◽

Vol 77 (05) ◽

Author(s):

MM Radwan ◽

RR Guzmán ◽

LC Fulks ◽

CL Burandt ◽

SA Ross

Keyword(s):

Antimicrobial Activities ◽

Phytochemical Study

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Biological Activities of Extracts from Ageratum fastigiatum: Phytochemical Study and In Silico Target Fishing Approach

Planta Medica ◽

10.1055/a-1576-4080 ◽

2021 ◽

Author(s):

Carolina Reis Cerqueira Sudan ◽

Lucas Campos Pereira ◽

Andréia Fonseca Silva ◽

Carolina Paula de Souza Moreira ◽

Denise de Oliveira Scoaris ◽

...

Keyword(s):

In Silico ◽

Biological Activities ◽

Ethanolic Extract ◽

Antimicrobial Activities ◽

Biological Properties ◽

Preparative Chromatography ◽

Phytochemical Screening ◽

Phytochemical Study ◽

Target Fishing

AbstractIn the present study, the ethanolic extract from aerial parts of Ageratum fastigiatum was evaluated in vitro against epimastigote forms of Trypanosoma cruzi (Y strain), promastigote forms of Leishmania amazonensis (PH8 strain), and L. chagasi (BH400 strain). The extract was also evaluated against Staphylococcus aureus (ATCC 25 923), Escherichia coli (ATCC 11 775), Pseudomonas aeruginosa (ATCC 10 145), and Candida albicans (ATCC 36 802). The phytochemical screening was performed by thin-layer chromatography and high-performance liquid chromatography. The extract was fractionated using flash preparative chromatography. The ethanolic extract showed activity against T. cruzi, L. chagasi, and L. amazonensis and antimicrobial activity against S. aureus, E. coli, P. aeruginosa, and C. albicans. The phytochemical screening revealed coumarins, terpenes/sterols, and flavonoids in the ethanolic extract. In addition, the coumarin identified as ayapin was isolated from this extract. We also performed in silico prediction of potential biological activities and targets for compounds previously found in A. fastigiatum. Several predictions were confirmed both retrospectively and prospectively by experimental results described here or elsewhere. Some activities described in the in silico target fishing approach were validated by the ethnopharmacological use and known biological properties. Some new activities and/or targets were predicted and could guide future studies. These results suggest that A. fastigiatum can be an interesting source of substances with antiparasitic and antimicrobial activities.

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