scholarly journals Tolerance and adaptation mechanisms developed by Moringa oleifera (L) seeds under oxidative stress induced by salt stress during in vitro germination

2021 ◽  
Keyword(s):  
Oxidative Stress ◽  
Moringa Oleifera ◽  
High Carbon ◽  
Total Polyphenols ◽  
Adaptation Mechanisms ◽  
Resistance To Stress ◽  
Moderate Resistance ◽  
Oxidative Species ◽  
Physiological And Biochemical

<p>The current study explores the capacities of Moringa oleifera (L), to overcome and/or tolerate oxidative stress induced by a saline substrate as a constraining factor. For this, Moringa seeds have been treated for 15 days after germination in vitro with increasing concentrations of NaCl, and specifically at 0 (control), 5; 7.5; 10; 12.5 and 15g l-1. Morpho-physiological and biochemical aspects were evaluated which were considered as probable indicators of tolerance or sensitivity to this stress. Our results reveal an increased synthesis of proline, lipids, proteins, CAT, GPX, APX, flavonoids, condensed tannins and a decrease in total polyphenols (-29.97%), mainly due to their high carbon cost. The state of degradation of the cell membranes was evaluated by the MDA assay, which increased by 91.25%. On the other hand, the evaluation of the oxidative damage caused by Reactive Oxidative Species (ROS) was detected by H2O2, which decreased by 37.89%. Additionally, our results revealed a disturbance of the germinative power of the seeds indicating moderate resistance to stress as it was manifested by the development of epicotyledonary axes, even under 15 g l-1 of NaCl without showing symptoms of stress or sensitivity. This result supports our hypothesis of the ability of the species to maintain or even improve its oxidative status.</p>

scholarly journals Moringa oleifera L. Extracts as Bioactive Ingredients That Increase Safety of Body Wash Cosmetics

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Zofia Nizioł-Łukaszewska ◽  
Dominika Furman-Toczek ◽  
Tomasz Bujak ◽  
Tomasz Wasilewski ◽  
Zofia Hordyjewicz-Baran
Keyword(s):  
Oxidative Stress ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Skin Irritation ◽  
Tree Leaves ◽  
Model Formulation ◽  
Different Types ◽  
Bioactive Ingredients ◽  
Positive Effect

The work attempts to obtain a multifunctional plant extract derived from Moringa tree leaves. Obtained results indicate a strong antioxidant potential of the tested extracts. It was shown that Moringa oleifera leaf extract is a rich source of flavonoid and phenolic compounds. Furthermore, it shows a strong antioxidant activity by scavenging free radicals. In vitro toxicity studies showed that the tested extracts in concentrations up to 5% showed a positive effect on cell proliferation and metabolism and may contribute to the reduction of oxidative stress in cells. It was noted that the tested model formulation of cosmetic (1% SCS) with the addition of different types of extracts might contribute to the reduction of skin irritation and improve the safety of the product.

scholarly journals Inactivation ofclpBin the Pathogen Leptospira interrogans Reduces Virulence and Resistance to Stress Conditions

2011 ◽  
Vol 79 (9) ◽  
pp. 3711-3717 ◽  
Author(s):  
Kristel Lourdault ◽  
Gustavo M. Cerqueira ◽  
Elsio A. Wunder ◽  
Mathieu Picardeau
Keyword(s):  
Oxidative Stress ◽  
Stress Conditions ◽  
Leptospira Interrogans ◽  
Wild Type ◽  
Content Type ◽  
General Stress Response ◽  
Growth Defects ◽  
Resistance To Stress ◽  
Increased Susceptibility

ABSTRACTLeptospira interrogansis the causative agent of leptospirosis, which is an emerging zoonotic disease. Resistance to stress conditions is largely uncharacterized for this bacterium. We therefore decided to analyze aclpBmutant that we obtained by random transposon mutagenesis. The mutant did not produce any of the two isoforms of ClpB. TheclpBmutant exhibited growth defects at 30° and 37°C and in poor nutrient medium and showed increased susceptibility to oxidative stress, whereas the genetically complemented strain was restored in ClpB expression andin vitrowild-type growth. We also showed that theclpBmutant was attenuated in virulence in an animal model of acute leptospirosis. Our findings demonstrate that ClpB is involved in the general stress response. The chaperone is also necessary, either directly or indirectly, for the virulence of the pathogenL. interrogans.

scholarly journals Rice Germosprout Extract Protects Erythrocytes from Hemolysis and the Aorta, Brain, Heart, and Liver Tissues from Oxidative StressIn Vitro

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Shahdat Hossain ◽  
Sujan Bhowmick ◽  
Marzan Sarkar ◽  
Mehedi Hassan ◽  
Jakir Hussain ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Lipid Peroxide ◽  
Erythrocyte Membranes ◽  
Total Polyphenols ◽  
Tissue Homogenates ◽  
Antioxidative Effects ◽  
Gallic Acid Equivalent

Identifying dietary alternatives for artificial antioxidants capable of boosting antihemolytic and antioxidative defense has been an important endeavor in improving human health. In the present study, we studied antihemolytic and antioxidative effects of germosprout (i.e., the germ part along with sprouted stems plus roots) extract prepared from the pregerminated rice. The extract contained considerable amounts of antioxidantβ-carotene (414±12 ng/g of extract) and phytochemicals such as total polyphenols (12.0±1.1 mg gallic acid equivalent/g of extract) and flavonoids (11.0±1.4 mg catechin equivalent/g of extract). The antioxidant potential of the extract was assessed by its DPPH- (2,2-diphenyl-1-picrylhydrazyl-) free radical scavenging activity where we observed that germosprout extract had considerable antioxidative potentials. To evaluate antihemolytic effect of the extract, freshly prepared erythrocytes were incubated with either peroxynitrite or Fenton’s reagent in the absence or presence of the extract. We observed that erythrocytes pretreated with the extract exhibited reduced degree ofin vitrohemolysis. To support the proposition that germosprout extract could act as a good antioxidative agent, we also inducedin vitrooxidative stress in erythrocyte membranes and in the aorta, brain, heart, and liver tissue homogenates in the presence of the extract. As expected, germosprout extract decreased oxidative stress almost to the same extent as that of vitamin E, as measured by lipid peroxide levels, in all the mentioned tissues. We conclude that rice germosprout extract could be a good natural source of antioxidants to reduce oxidative stress-induced hemolysis and damage of blood vessels and other tissues.

Anti-Acute Myeloid Leukemia Activity of Chaetocin, a Novel Epigenetic Drug Inhibitor Inducing Oxidative Stress.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 889-889
Author(s):  
Hassiba Chaib ◽  
Thomas Prebet ◽  
Audrey Restouin ◽  
Remy Castellano ◽  
Sandrine Opi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Caspase 3 ◽  
Hdac Inhibitors ◽  
Oxidative Species ◽  
Acute Myeloid

Abstract Recent studies have highlighted the importance of epigenetic modifications in the pathogenesis of Acute Myeloid Leukemia (AML). This results have been confirmed by the activity of new drug like DNA demethylating agents and histone deacetylase (HDAC) inhibitors in both in vivo and in vitro studies. Recently, Chaetocin, a natural fungal compound, has been identified as the first specific inhibitor of the histone methyltransferase SU(VAR)3–9 which plays a role in heterochromatin gene silencing. In this study, we decided to evaluate Chaetocin as a therapeutic agent in AML in vitro and to explore the related mechanisms. We show that Chaetocin induce dramatic cell death at nanomolar concentrations in U937 and HL60 (97.2% ± 0.4 and 91.6% ± 9 cell death at 100 nM chaetocin, respectively), and to a lesser extend in K562 (67.3% ± 1.6 cell death at 100 nM chaetocin), cell cultures. Cell death occurred at 24 h incubation time which correlated with induction of apoptosis as assessed by Annexin V/7-AAD staining and activation of downstream executioner caspase-3/7. Using transcription low-density array and quantitative RT- PCR, Chaetocin was showed to up-regulate gene transcription such as of the cell cycle inhibitor p21/WAF1 consistent with a role for the targeted SU(VAR)3–9 in heterochromatin gene silencing. In agreement with the recent report of Chaetocin being a promising new antimyeloma agent acting via imposition of oxidative stress, intracellular levels of oxidative species were increased in Chaetocin treated U937 cells in a time- and dose-dependent manner that correlated with induction of cell death. Furthermore, incubation of cells with N-acetyl cysteine, a cell-permeable precursor of intracellular glutathione reductant, prevented chaetocin-induced accumulation of oxidative species, transcription of selected genes (e.g. p21/WAF1), activation of caspase-3, and cell death. Finally, Chaetocin was found to increase the antileukemia activity of HDAC inhibitors and Aracytin, and thus appears as a promising agent for further study as a potential anti-AML therapeutic. Preliminary results obtained in vivo in xenograft models and ex vivo, using blasts of a panel of patients with AML, will be presented.

scholarly journals Physiological and biochemical mechanisms of plants resistance to oxidative stress under peptide elicitor AtPep1

2021 ◽  
pp. 38-46
Author(s):  
Halina G. Filiptsova ◽  
Vladimir M. Yurin
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Aerial Part ◽  
Lipid Peroxidation Products ◽  
Resistance To Stress ◽  
Biochemical Mechanisms ◽  
Physiological And Biochemical Mechanisms ◽  
Physiological And Biochemical

The effect of the peptide elicitor AtPep1 on the resistance of soybean and pea plants to oxidative stress was studied. The concentration of the peptide 10– 9 mol/L has the maximum elicitor effect on these plants. It was shown that treatment of the aerial part of seedlings with this peptide leads to an increase in the activity of peroxidase and superoxide dismutase and a decrease in the level of lipid peroxidation products in plants under oxidative stress. Revealed effects cause an increase in the plants resistance to stress.

Interactions Between Dental Composite Resins and Saliva A comparative biochemical in vitro study

2019 ◽  
Vol 56 (3) ◽  
pp. 529-533
Author(s):  
Mihaela Pantea ◽  
Diana Andreea Ighigeanu ◽  
Alexandra Totan ◽  
Maria Greabu ◽  
Daniela Miricescu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Study ◽  
Composite Resins ◽  
Vitro Study ◽  
Dental Composite ◽  
Gamma Glutamyl Transferase ◽  
Specific Protocol ◽  
Dental Composite Resins ◽  
Glutamyl Transferase

This in vitro study analyses the biochemical interaction between saliva and three types of dental composite resins (a direct resin, an indirect resin and a dual-cure resin used for cementation of indirect dental restorations). The resin samples were obtained following a specific protocol and in line with the producers� recommendations; the resin samples were incubated with saliva samples collected from 19 healthy volunteers. The obtained results showed that the tested composite resins did not produce significant changes in oxidative stress parameters that were analysed (albumin, uric acid, GGT / gamma glutamyl transferase, OXSR-1 / oxidative stress responsive kinase 1) and do not influence the inflammatory salivary status reflected by the levels of IL-6 - an inflammatory marker.

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