scholarly journals Chromium (III) induced haematological alterations in Indian common carp, Labeo rohita (Ham.)

2011 ◽  
Vol 3 (2) ◽  
pp. 258-263 ◽  
Author(s):  
N. V. Bhatkar
Keyword(s):  
Leucocyte Count ◽  
Labeo Rohita ◽  
Haemoglobin Concentration ◽  
Erythrocyte Count ◽  
Water Bodies ◽  
Diagnostic Tools ◽  
Total Leucocyte Count ◽  
Corpuscular Haemoglobin ◽  
Chromium Chloride ◽  
Mean Corpuscular Haemoglobin

The principle source of chromium in water bodies is discharged from industries which use large amount of chromates or dichromates. The freshwater fish, Labeo rohita was used as a model in the present studies to investigate the responses to chromium metal salt contamination in water bodies as it is the most common fish consumed widely in India. Haematological tests were carried out as these are the important diagnostic tools and are equally valuable as indicators of disease or stress due to pollutants and environmental fluctuations. The fishLabeo rohita were exposed to sublethal concentration of chromium chloride for 10, 20 and 30 days to study alterations in certain haematological parameters. The parameters under study were total erythrocyte count (TEC), haemoglobin (Hb gm%), total leucocyte count (TLC), differential leucocyte count (DLC) and pack cell volume (PCV). The studies revealed that chromium chloride-treated Labeo rohita exhibited decreased levels of total erythrocyte count (TEC), haemoglobinn (Hb gm%) and PCV whereas, total leucocyte count (TLC) was increased under thestress of chromium metal The decreased WBC count found after 30 days along with depleted Hb content and RBC count indicate dysfunctioning of haemopoietic systems along with dysleucopoiesis. Leucocytosis observed after 10 and 20 days exposure has been considered to be an adaptation to meet stressful conditions by animals. Increase in macrophages and basophils appears to be protective response during chromium exposure. An increasing trend in the mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were evident in fish exposed to sublethal doses of chromium chloride when compared with the control.

scholarly journals Haematological responses of the Indian major carp Labeo rohita to Saprolegniasis

2017 ◽  
Vol 64 (2) ◽  
Author(s):  
Chandan Debnath ◽  
Basanta Kumar Das ◽  
Lopamudra Sahoo
Keyword(s):  
Labeo Rohita ◽  
Haemoglobin Concentration ◽  
Infected Fish ◽  
Control Group ◽  
Total Leucocyte Count ◽  
Blood Profile ◽  
Corpuscular Haemoglobin ◽  
Indian Major Carps ◽  
Management Measures ◽  
Mean Corpuscular Haemoglobin

Saprolegniasis (cotton wool disease), due to infestation by Saprolegnia parasitica, is a major fungal disease in fish. Present study was conducted in the Indian major carps, rohu, Labeo rohita (Hamilton, 1822). Fingerlings of rohu (10.5±2.2 g) were artificially infected with the fungal parasite S. parasitica via injection (T1), cohabitation (T2) as well as immersion (T3) and compared with a control group (T0) with no infection. Blood samples were collected at 15 days interval over a period of 45 days and analysed for haematological parameters. Haemoglobin level in infected fish was lower (p≤0.05) than non-infected fish on 15, 30 and 45 days post-infection (dpi) except in cohabited fish on 45 dpi. Total erythrocyte count (TEC) in infected fish was lower (p≤0.05) than non-infected fish on 15, 30 and 45 dpi. Total leucocyte count (TLC) was higher (p≤0.05) in infected fish on 15 dpi; however no difference (p≥0.05) was noticed on 30 dpi. TLC in injected group was higher (p≤0.05) than control group on 45 dpi. Haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and leucocrit (Lct) values in infected group were lower (p≤0.05) than control group. There was no difference (p≥0.05) in mortality among the groups on 15 dpi; however, on 30 and 45 dpi, the group subjected to immersion exposure recorded highest mortality followed by injected and cohabitated groups. Varying degrees of histological alterations were observed in the tissues due to the fungal infestation. The results indicated that S. parasitica significantly altererd the blood profile of infected rohu leading to 45-50% mortality. Therefore appropriate management measures need to be adopted for preventing infestation by S. parasitica in carp culture systems for realising sustainable fish production.

scholarly journals Influences of breed, sex and age on seasonal changes in haematological variables of tropical goat kids

2017 ◽  
Vol 60 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Buhari Habibu ◽  
Mohammed Kawu ◽  
Hussaina Makun ◽  
Tagang Aluwong ◽  
Lukman Yaqub ◽  
...  
Keyword(s):  
Seasonal Changes ◽  
Haemoglobin Concentration ◽  
Dry Season ◽  
Haematological Parameters ◽  
Total Leucocyte Count ◽  
Corpuscular Haemoglobin ◽  
Hot Dry Season ◽  
Mean Corpuscular Haemoglobin ◽  
Sahel Goat ◽  
Dry And Rainy Seasons

Abstract. The influences of breed, sex and age on seasonal changes in haematological variables of kids (1–4 months old) belonging to Red Sokoto (n  =  60) and Sahel (n  =  60) goats were studied at the peaks of the cold-dry, hot-dry and rainy seasons in a West Africa Guinea savanna climate. The results showed that, during the hot-dry season, Sahel goat kids had significantly higher (P  <  0.05) packed cell volume (PCV) and red blood cell (RBC) count but lower (P  <  0.05) mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and total leucocyte count than Red Sokoto kids. Similarly, younger kids of both breed had significantly higher (P  <  0.05) PCV and RBC but lower MCV, MCH and MCHC compared with the older kids during the hot-dry season. Younger kids (1–2 months) of both breeds exhibited significantly (P  <  0.05) high PCV and RBC but low MCH and MCHC during the hot-dry season as compared with the cold-dry and rainy seasons, with the magnitude of the change being greater in Sahel kids. More remarkable seasonal fluctuations in haematological parameters were observed in buck kids than doe kids of both breeds. Multivariate analysis revealed a clear distinction between the change in haematological parameters during the cold-dry as compared with the hot-dry and rainy seasons, with MCV having the strongest discriminating power (0.91*). In conclusion, breed, age and sex variations in haematological variables of goats were more pronounced in the hot-dry season, during which the seasonal changes were more dramatic in kids of Sahel goats, as well as in younger and male kids of both breeds. These findings may be useful in the management of tropical goat kids in different seasons.

scholarly journals SEASONAL VARIATION IN THE LEVELS OF SOME CHEMICAL AND HAEMATOLOGICAL COMPONENTS IN THE BLOOD OF WHITE FULANI COWS IN WESTERN NIGERIA

2021 ◽  
Vol 4 (2) ◽  
pp. 11-17
Author(s):  
S. K. Olusanya
Keyword(s):  
Water Content ◽  
Mean Corpuscular Volume ◽  
Haemoglobin Concentration ◽  
Total Serum Protein ◽  
Total Serum ◽  
Total Leucocyte Count ◽  
Corpuscular Volume ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin ◽  
White Fulani

Haematological and some biochemical determinations were made on the blood of six White Fulani heifers monthly for a period of twelve months in order to investigate any normal seasonal variations in the levels of some of these components. It was found that packed cell volume, haemoglobin and red cell count values were higher during the relatively dry months of the year than the wet months. These changes can possibly be explained by change in the water content of the serum since body water content would decrease with decrease in haemoconcentration. Total leucocyte count, mean corpuscular haemoglobin concentration and total serum protein tended to increase during the rains and were lower in the dry months, while there were no appreciable seasonal differences observed in the levels of mean corpuscular volume, mean corpuscular haemoglobin, inorganic phosphorus and calcium. However, mean corpuscular volume appeared to increase with increasing age

scholarly journals Erythrocyte Profile of Diploid and Triploid Silver Crucian Carp (Carassius auratus)

2006 ◽  
Vol 75 (2) ◽  
pp. 203-207 ◽  
Author(s):  
L. Vetešník ◽  
K. Halačka ◽  
V. Lusková ◽  
S. Lusk
Keyword(s):  
Ploidy Level ◽  
Mean Corpuscular Volume ◽  
Carassius Auratus ◽  
Haemoglobin Concentration ◽  
Mean Value ◽  
Erythrocyte Count ◽  
Corpuscular Volume ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin ◽  
Haemoglobin Content

Haematological analysis was performed on 27 adult specimens of Carassius auratus irrespective of sex in 2003 and on 32 juveniles of distinguished sex in 2004. In this study we found that the ploidy level affected significantly (p < 0.01) the values of the erythrocyte count, mean corpuscular volume and mean corpuscular haemoglobin. Although we did not prove any significant effect of sex in juvenile diploids of C. auratus on the values of erythrocyte profile, the erythrocyte count, haematocrit value and haemoglobin content value were higher for males than for females. The erythrocyte count decreased significantly (p < 0.01) with increasing ploidy level. The index of haemoglobin content followed the same trend of a decreasing mean value with increasing ploidy level. Mean corpuscular volume and mean corpuscular haemoglobin increased with the increasing ploidy level (p < 0.01). Haematocrit value and mean corpuscular haemoglobin concentration did not significantly differ from the point of view of the ploidy level.

scholarly journals Nitrite Intoxication of Common Carp (Cyprinus carpio L.) at Different Water Temperatures

2006 ◽  
Vol 75 (4) ◽  
pp. 561-569 ◽  
Author(s):  
H. Kroupová ◽  
J. Máchová ◽  
V. Piačková ◽  
M. Flajšhans ◽  
Z. Svobodová ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Haemoglobin Concentration ◽  
Nitrite Accumulation ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin ◽  
Higher Temperature ◽  
Lower Temperature ◽  
Plasma Nitrite

Common carp (Cyprinus carpio L.) were exposed to nitrite (1.45 mmol l-1 NO2-) for 48 hours at 14 °C and 20 °C, in order to investigate the mechanism of nitrite poisoning at these water temperatures. The effect of nitrite exposure on fish was assessed on selected haematological and biochemical indicators of the blood. Moreover, nitrite accumulation in the blood, liver and muscle was measured. Nitrite exposure produced high levels of methaemoglobin (88.2 ± 3.3% and 92.9 ± 6.1%) at both water temperatures compared with controls (0.3 ± 0.6% and 2.6 ± 3.0%). High fish mortality occurred in experimental groups (30% and 51%) compared with controls (0%). Nitrite exposure also resulted in an accumulation of nitrite in the fish body. The highest nitrite levels developed in the blood plasma, followed by the liver and muscle, respectively. Carp concentrated nitrite in the blood plasma and tissues to markedly higher levels at higher temperature (20 °C). The plasma nitrite concentrations (10.5 ± 1.9 mmol l-1) were in this case more than 7 times higher than the environmental one. At lower temperature (14 °C), plasma nitrite concentration reached 5.0 ± 1.5 mmol l-1. In either event, plasma K+ levels increased and Cl- levels and osmolality remained unchanged. Plasma Na+ levels slightly decreased at the higher temperature. Nitriteexposed fish showed lower haematocrit values (PCV) at both experimental temperatures compared with controls. At 20 °C, the blood haematocrit decrease (0.20 ± 0.02 l l-1) was accompanied by a low erythrocyte count (1.05 ± 0.12 1012 l-1) and by a low haemoglobin level (51 ± 11 g l-1). At the lower temperature (14 °C), the haematocrit decrease (0.25 ± 0.02 l l-1) was caused by a low mean corpuscular volume (167 ± 27 fl). No significant changes were observed in the mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), or selected erythrocyte dimensions (major axis, minor axis and aspect ratio).

scholarly journals Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Y. Garba ◽  
E. A. Adeola
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Corpuscular Haemoglobin ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Mean Corpuscular Haemoglobin ◽  
Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

Haematological Profiles of Pigs on Different Farms Reflect Their Health Status

2021 ◽  
Author(s):  
Irena Golinar Oven ◽  
Alenka Nemec Svete ◽  
Melita Hajdinjak ◽  
Jan Plut ◽  
Marina Stukelj
Keyword(s):  
Health Status ◽  
Blood Cell ◽  
Cell Count ◽  
White Blood Cell ◽  
Haemoglobin Concentration ◽  
Haematological Parameters ◽  
Blood Cell Count ◽  
Blood Samples ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin

Abstract BackgroundHaematological examination is an important diagnostic tool in the assessment of pig health status. The present study aimed to assess haematological parameters in pigs of different age categories from six farrow-to-finish farms differing in herd health status. The following pig categories were included: 5 age groups of growers (5, 7, 9–10, 11 and 12–13 weeks-old), fatteners and breeding pregnant sows. Individual blood samples for determining complete blood count and white blood cell differential count were taken and group samples of oral fluid and faeces were collected from each animal category in each of the six farms and tested for the detection of Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Virus (PRRSV), and Hepatitis E Virus (HEV) using PCR, RT-PCR, and qRT-PCR protocols. Individual blood samples were analysed using an automated laser-based haematology analyser. The following haematological parameters were reported: white blood cell count (WBC), red blood cell count (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet count (PLT), and percentage and number of neutrophils, lymphocytes, monocytes, eosinophils, basophils, and "large unstained cells" - LUCs. ResultsOn farms free of PRRSV, PCV2 and HEV, age had significant effect on the following parameters: WBC, Hb, MCH, MCHC, PLT, percentage of neutrophils, lymphocytes and eosinophils and absolute numbers of neutrophils, lymphocytes, monocytes, basophils and LUCs. On farms with PRRS, PCV2 and/or HEV, age significantly affected all observed blood parameters except the percentage of LUCs. The percentages of lymphocytes, MCV and Hct were significantly lower by PRRSV while WBC, PLT, percentage and absolute number of neutrophils, basophils and LUCs increased. Significantly lower percentages of lymphocytes and increased percentages and absolute numbers of neutrophils, eosinophils and basophils were caused by PCV2 presence. Significantly lower percentage of lymphocytes and MCV and increased RBC, Hb, percentage and number of basophils and percentage of neutrophils were caused by HEV. Conclusions Alterations of haematological parameters reflected the health status of pigs of different categories on infected and on non-infected farms. Age-related changes in haematological parameters occurred in clinically healthy and in infected pigs.

Ecotoxicological assessment of commercial glyphosate-based di-ammonium salt formulation on haematological indices of Clarias gariepinus

2021 ◽  
Vol 36 (1) ◽  
pp. 13-21
Author(s):  
A.B. Ella ◽  
E.T. Azua ◽  
C.U. Aguoru ◽  
A.A. Onekutu ◽  
F.A. Ella
Keyword(s):  
Blood Cell ◽  
Clarias Gariepinus ◽  
Haemoglobin Concentration ◽  
Negative Consequences ◽  
Ecotoxicological Assessment ◽  
Corpuscular Haemoglobin ◽  
Physico Chemical ◽  
Haematological Profile ◽  
Mean Corpuscular Haemoglobin ◽  
Total Dissolved Solid

The study evaluated sub-lethal toxicity of commercial glyphosate-based Di-ammonium formulation commonly known as ‘Touchdown’ on haematological profile of Clarias gariepinus juveniles. A total of two hundred catfish juveniles of mean weight 31.07±1.23g and mean length 19.50 ± 0.50cm were exposed to different  concentrations of Di-ammonium formulation (1.26, 2.52, 5.03 and 0.00 mg/l) for eight (8) weeks. Each concentration was treated in triplicate using a static bioassay system. Physico-chemical parameters of the test water were monitored throughout the study period. Results showed that haematocrit (HCT), red blood cell (RBC), haemoglobin (HGB), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC) and Platelets (PLT) counts decreased significantly (p<0.05) with increase in concentrations of Touchdown. Whereas, white blood cell (WBC) counts increased significantly (p<0.05) with increasing concentrations of the glyphosate-based formulation. Water temperature, pH, Total dissolved solid (TDS) and electrical conductivity (EC) increased linearly with increasing concentration, while dissolved oxygen content decreased significantly (p<0.05). The results from this study indicated that Di-ammonium glyphosate–based formulation had negative consequences on the blood of treated fish. In view of this, the application of Di-ammonium glyphosate-based formulation should be done in a sustainable manner with proper regulations.

scholarly journals The Haematology and Serum Biochemistry Profile of Adult Japanese Quail (Coturnix coturnix japonica)

2017 ◽  
Vol 9 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Onyinyechukwu A. AGINA ◽  
Wilfred S. EZEMA ◽  
Ezinwa M. IWUOHA
Keyword(s):  
Uric Acid ◽  
Total Cholesterol ◽  
Haemoglobin Concentration ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Serum Biochemistry ◽  
Total Proteins ◽  
Corpuscular Haemoglobin ◽  
Coturnix Coturnix ◽  
Mean Corpuscular Haemoglobin

The present study established the reference values and sex differences in the erythrocytic and serum biochemistry parameters of domestic adult quails (Coturnix coturnix). Ninety five adult birds, comprising of 42 males and 53 female Japanese quails were sampled using a simple random sampling technique. Standard procedures were carried out in all haematology and serum biochemistry determinations. The overall mean for the erythrocytic and serum biochemistry parameters were as follows: packed cell volume (PCV) 43.11%, red blood cell count (RBC) 4.31 × 106/µl, haemoglobin concentration (Hbc) 16.21 g/dl, mean corpuscular volume (MCV) 100.69 fl, mean corpuscular haemoglobin (MCH) 39.17 pg, mean corpuscular haemoglobin concentration (MCHC) 39.35 g/dl, aspartate aminotransferase (AST) 59.99 IU/L, alanine aminotransferase (ALT) 20.85 IU/L, alkaline phosphatase (ALP) 107.54 IU/L, total proteins (TP) 5.19 g/dl, albumin (ALB) 3.25 g/dl, globulin (GLB) 1.94 g/dl, albumin: globulin 1.73, total cholesterol (TCHOL) 146.69 mg/dl, total bilirubin (TBIL) 2.37 mg/dl, uric acid (UA) 16.02 mg/dl and creatinine (CREAT) 0.44 mg/dl. The PCV of the males were significantly higher than that of the females, while the MCH and MCHC of the females were significantly higher than those of the males. The serum total proteins, albumin, globulin, uric acid, creatinine, and total cholesterol values of the female quails were higher than those of the male quails. The present data might be useful to avian specialists and veterinary clinicians, but more research works should be carried out on quails to increase the information data base, especially in the tropics.

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