scholarly journals Respon Pemberian Hormon 2,4-D dan BAP terhadap Pertumbuhan Subkultur Kalus Kedelai (Glycine max (L.) Merrill) secara In Vitro

Biosfera ◽  
2015 ◽  
Vol 32 (1) ◽  
pp. 59
Author(s):  
Kiki Ayuningrum ◽  
Iman Budisantoso ◽  
Kamsinah Kamsinah
Keyword(s):  
Glycine Max ◽  
Combination Treatment ◽  
Dry Weight ◽  
Soybean Callus ◽  
Randomized Design ◽  
Glycine Max L ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Callus Type

The purpose of this study was to evaluate the response of administration of a combination of 2,4-D and BAP on the growth of soybean callus subculture and determine the combination of 2,4-D and BAP most good for the growth of soybean callus subculture. The study design used completely randomized design (CRD) with a pattern factorials. The factor one e.i 2,4-D consists of 4 levels, namely: 0, 5, 10, and 15 ppm. A factor of 2 e.i BAP consists of 4 levels, namely: 0, 2, 4, dan 6 ppm. Every combination treatment repeated three times. Parameters measured include the percentage is growing callus, type of callus, dry weight and wet weight of soybean callus. The results showed that administration of the hormone 2,4-D and BAP can spur the growth of soybean callus subculture, the combination of BAP 2 ppm and 10 ppm of 2,4-D is the best combination for a percentage of callus and growing callus types, whereas the wet weight and the weight dried callus is not driven by a combination of the hormone 2,4-D and BAP

The Effect Of Cow State Fertilizer with Various Bioactivations and Cow Broth Feeding On The Growth and Production Of Soybean (Glycine Max L. Merril).

Nabatia ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Siska Melinda
Keyword(s):  
Glycine Max ◽  
Root Nodules ◽  
Dry Weight ◽  
Growth And Yield ◽  
Experimental Unit ◽  
Cow Manure ◽  
Randomized Design ◽  
Glycine Max L ◽  
Completely Randomized Design ◽  
Number Of Branches

The Effect of Cow Manure With Various Bioactivators And Giving Beef Broth To The Growth And Yield Of Soybeans (Glicine Max L. Merril) Supervised by Dwi fitriani, SP. MP and Dr.Ir.Ririn Harini,MP. The purpose of this study was to determine the interaction and effect of cow manure with various bioactivators and broth. This research was conducted in Pondok Kubang District, Central Bengkulu Regency, Bengkulu Province. The design used was a completely randomized design (CRD) factorial treatment 1, namely cow manure with various bioactivators A0 (Control) A1 (Yeast) A2 (Stale Rice) A3 (Rumen). The second treatment was giving control broth, 100 ml/L, 150 ml/L, 200 ml/L. Each treatment was repeated 3 times in order to obtain 48 experimental units by planting 3 plants for each experimental unit to obtain 144 plants. The results showed that the treatment of cow manure with various bioactivators had a significant effect on the parameters of leaf number 56 DAP, number of branches 56 DAP, number of root nodules, number of pithy pods, dry weight of planting, weight of planted seeds, weight of 100 seeds. and has not shown any effect on other parameters.

scholarly journals RESPON BEBERAPA VARIETAS KEDELAI (GLYCINE MAX (L.) MERRIL) TERHADAP INFEKSI PEANUT STRIPE POTYVIRUS

2002 ◽  
Vol 2 (2) ◽  
pp. 47-50
Author(s):  
Hasriadi Mat Akin ◽  
Muhammad Nurdin
Keyword(s):  
Glycine Max ◽  
Incubation Period ◽  
Dry Weight ◽  
Growth And Yield ◽  
Soybean Plant ◽  
Soybean Yield ◽  
Randomized Design ◽  
Glycine Max L ◽  
Completely Randomized Design ◽  
Plot Experiment

Response of various soybean varieties [Glycine max (L.) Merril] againts peanut stripe potyvirus infection. This experiment was conducted to investigate the effect of PStV infection on soybean growth of various soybean varieties. Treatments were arranged in completely randomized design in split plot experiment with four replications. Main plots were virus-inoculated and uninoculated plants; subplots were eight soybean varieties (Wilis, Orba, MLG2521, Amerikana, Yellowbean, B3570, Slamet and Taichung). The results showed that infection decreased soybean growth indicated by reduction dry weight of soybean plant and reduction of soybean yield. Slamet variety compared to other varieties showed susceptible reaction indicated by the shortest incubation period (9,75 days), significant reduction of dry weigh of soybean plant, and the highest reduction of soybean yield (23,6%). Thaicung variety showed resistant reaction indicated by longest incubation period (14,3 days) and insignificant reduction of soybean growth and yield.

scholarly journals PENGARUH PENYIMPANAN SAMPAI ENAM BULAN MENGGUNAKAN BUBUK DAN TANPA BUBUK LADA TERHADAP MUTU LIMABELAS LOT BENIH KEDELAI (Glycine max L.)

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Ayu Kurniati ◽  
Agustiansyah Agustiansyah ◽  
Yayuk Nurmiaty ◽  
Ermawati Ermawati
Keyword(s):  
Glycine Max ◽  
Combination Treatment ◽  
Room Temperature ◽  
Seed Quality ◽  
Soybean Seeds ◽  
Randomized Design ◽  
Temperature Ranges ◽  
Glycine Max L ◽  
Completely Randomized Design ◽  
Pepper Powder

This study aims to determine the best seed quality from fifteen lots of soybean seeds that are stored for up to six months using pepper powder and without using pepper powder. The treatment was applied in a Completely Randomized Design (CRD) which was repeated three times. The combination of treatments derived from three varieties, namely Anjasmoro, Gerobogan, and Burangrang combined with 5 SP-36 fertilizer doses, namely 0, 100, 150, 200, and 250 kg / ha. The experiment consisted of two experiments. Experiment 1, the fifteen lots of seeds were stored without pepper powder. Experiment 2, the fifteen lots of seeds were ground with pepper in their storage. Storage at room temperature ranges from 28-30oC in the Seed and Plant Breeding Laboratory, Faculty of Agriculture, University of Lampung in October 2017 to April 2018 The results showed that the combination treatment of Burangrang varieties with SP-36 250 kg / ha fertilizer stored without pepper powder or pepper powder was able to maintain its higher quality compared to other treatment combinations after being stored for up to six months Key words: Fertilizer, pepper powder, soybean, storage, variety

Respon Akar Bibit Aren Genjah (Arenga pinnata) Di Pembibitan Pada Pemberian Dosis dan Interval Pupuk Organik Cair Nasa

2018 ◽  
Vol 7 (1) ◽  
pp. 28
Author(s):  
Yetti Elidar
Keyword(s):  
Organic Fertilizer ◽  
Dry Weight ◽  
Treatment Combination ◽  
Root Dry Weight ◽  
Randomized Design ◽  
Dose Study ◽  
Palm Sugar ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Leaf Dry Weight

Research on the response of roots of palm sugar palm seeds (Arenga pinnata) in nurseries at doses and intervals of Nasa liquid organic fertilizer. Aims to determine the dosage, interval and combination of dosages and fertilization intervals with Nasa liquid organic fertilizer which can provide the best dry weight of the roots in the nursery. The research design used was a Completely Randomized Design (CRD) with 3x3 factorial experiments and each treatment was repeated 8 (eight) times, consisting of: the first factor was the treatment of POC Nasa dose in a concentration of 3 cc POC Nasa per liter of water (D) consists of 3 levels, namely: d1 = 300 ml POC Nasa, d2 = 400 ml POC Nasa, d3 = 500 ml POC Nasa, while the second factor is the treatment of POC Nasa Interval (I) consisting of 3 levels, namely: i1 = 2 once a week, i2 = once every 3 weeks, i3 = once every 4 weeks. The results of the POC Nasa dose study had a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level are: d2 (400 ml of Nasa liquid organic fertilizer), the interval of liquid organic fertilizer Nasa has a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level is: i1 (once every 2 weeks). The treatment combination has no significant effect on all parameters. 

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

Pengaruh Penambahan Air pada Pengolahan Susu Kedelai

2015 ◽  
Vol 4 (1) ◽  
pp. 8-13
Author(s):  
Priscillia Picauly ◽  
Josefina Talahatu ◽  
Meitycorfrida Mailoa
Keyword(s):  
Glycine Max ◽  
Physical Properties ◽  
Sweet Taste ◽  
Soya Milk ◽  
Randomized Design ◽  
Soybean Extract ◽  
Glycine Max L ◽  
Completely Randomized Design ◽  
Water Ratio

Soybean (Glycine max (L) Merr) is needed as an alternative diversification in order to increase protein consumption. Soybean can be processed into variety of foods and drinks. The most popular drink from soybean is soya milk which is made from soybean extract yielded from the addition of water. The amount of water added determine the quality of soya milk due to its effect on the chemical and physical properties of soya milk. This research was intended to find out the proper ratio of soybean and water in the processing of soya milk to yield the best quality. A completely randomized design was used with eight treatments of soybean and water ratio as follows, ratio of 1 : 6, ratio of 1 : 8, ratio of 1 : 10, ratio of 1 : 12, ratio of 1 : 14, ratio of 1 : 16, ratio of 1 : 18 and ratio of 1 : 20. Result showed the soybean and water ratio 1 : 10 in the processing of soya milk yielded milk having good quality according to its chemical with protein content of 2.53%, fat of 1.20%, sugar total of 1.60%, pH of 7.1, and organoleptic characteristies of both somewhat sweet taste and viscous texture.

scholarly journals Promoting Tuber Formation In Vitro with Benzyl Amino Purine and Paclobutrazol at Different Concentrations

Akta Agrosia ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 29-35
Author(s):  
Usman Kris Joko Suharjo ◽  
Hasanudin Hasanudin ◽  
Tunjung Pamekas ◽  
Hesti Pujiwati ◽  
Alyi Vanturini
Keyword(s):  
Poor Quality ◽  
Tuber Formation ◽  
Potato Tubers ◽  
Seed Sources ◽  
Randomized Design ◽  
Plant Culture ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Range Test

A common problem related to the production of potatoes in Indonesia is the use of poor quality seed sources and declining seeds. Plant tissue culture is one of the best methods used for the provision of quality seeds. This study aims to establish the best combination of BAP concentration and the concentration of paclobutrazol in spurring the growth of cuttings and initiation of potato tubers in vitro.This research was conducted in November 2017 until June 2018 at Agronomy Laboratory of Biotechnology Division and Plant Culture Network of Agriculture Faculty of Bengkulu University. The  research  design  used  was  Completely  Randomized  Design  (CRD)  which  consist  of  18 combination of BAP treatment and Paclobutrazol was repeated 6 times. The concentration of BAP used was 0 mg / l, 5 mg / l, 10 mg / l and the concentration of Paclobutrazol 0 mg / l; 2.5 mg / l; 5 mg / l; 7.5 mg / l; 10 mg / l; 12.5 mg / l. The data of the research were analyzed by F test of 5% level and if there were different between treatments followed by Duncan's Multiple Range Test (DMRT) test of5% level. The results showed that the combination of BAP and  Paclobutrazol concentration had significant effect on all observed variables. Treatment of 5 mg / l BAP and 7.5 mg / l Paclobutrazol is the best medium in inducing potato micro tube as indicated by the fastest tuber formation time, highest percentage of productive crops, largest number of tubers per bottle, number of tubers per productive plant, tuber diameter the largest and highest wet weight of tuber per bottle. Keywords : in vitro, BAP, paclobutrazol, Potato

scholarly journals ​The Effect of Rhizobacteria in the Reducing drought Stress in Soybean (Glycine max L.)

2021 ◽  
Author(s):  
E. Oral ◽  
R. Tunçtürk ◽  
M. Tunçtürk
Keyword(s):  
Glycine Max ◽  
Green Algae ◽  
Dry Weight ◽  
Water Restriction ◽  
Blue Green Algae ◽  
Physiological Properties ◽  
Seedling Length ◽  
Glycine Max L ◽  
Wet Weight ◽  
Irrigation Levels

Background: This study was carried out to determine the effects of rhizobacteria and blue green algae applications on some physiological properties of soybean (Glycina max L.) grown under water stress. Methods: The experiment was conducted in factorial order with 4 replications according to the randomized plot trial design. In the research, Arýsoy variety belonging to soybean (Glycine max L.) species was used. In the experiment, it was aimed to examine the effects of three different irrigation levels (100, 50 and 25%) on some physiological characteristics of soybean with a control (control (B0), two different bacteria used. In this study, strains R1 of Azospirillum lipoferum bacteria, strains numbered 98 belonging to Bacillus megaterium bacteriaein and one blue green algae (Chlorella saccharophilia) were used. Result: According to the average data obtained, root length is 24.75 cm- 30.85 cm, seedling length 28.10-36.57 cm, root fresh weight 1.10-1.43 g, seedling wet weight 1.55-2.41 g, root dry weight 0.15-0.18 g, seedling dry weight 0.38-0.46 g, azote balance index 70.64-82.90 (dualex value), flavonol 0.375-0.398 (dualex value) and anthocyanin 0.016-0.045 (dualex value), with water restriction showed a decrease in most of these values. It has been determined that the rhizobacteria and blue-green algae have a decreasing and regulating effect on the physiological properties examined.

scholarly journals The Utilization of Effective Microorganism 4 (EM4) on Growth of Oil Palm Seedling in Pre Nursery

2020 ◽  
Vol 3 (2) ◽  
pp. 39
Author(s):  
Dwi Astutik ◽  
Ratih Rahhutami ◽  
Aline Sisi Handini ◽  
Ahmad Sutopo
Keyword(s):  
Oil Palm ◽  
Research Method ◽  
Dry Weight ◽  
Shoot Dry Weight ◽  
Randomized Design ◽  
Significant Difference ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Effective Microorganism

<em>A planting medium that rich in nutrients is needed for oil palm, especially in the nursery phase. This study aimed to determine the effect of various doses of EM4 and to get the best dose of EM4 on the growth of oil palm seedlings in the pre-nursery. The research method used was a non-factorial completely randomized design with 4 treatments, namely 0 ml EM4 (A1), 4 ml EM4 (A2), 8 ml EM4 (A3), and 12 ml EM4 (A4). Each treatment was repeated 6 times so that there were 24 experimental units. The data obtained were analyzed for variance at the 5% level, if the effect was real, it was followed by the least significant difference (LSD) test. Based on the results of the study, it could be concluded that application of various EM4 doses had a significant effect on plant height (4-12 MST), the number of leaves (4-8 MST), trunk girth (4-12 MST), and shoot wet weight but did not significantly affect the number of leaves (12 MST), the volume of roots, length of roots, root wet weight and shoot dry weight, dry weight root, the best dose was shown at a dose of 12 ml EM4.</em>

scholarly journals Respon Pertumbuhan dan Produksi Andrographolida Tanaman Sambiloto (Andrographis paniculata Nees) Akibat Perbedaan Dosis Pupuk Guano

Rekayasa ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 164-171
Author(s):  
Suhartono Suhartono ◽  
Diana Nurus Sholehah ◽  
Rohmad Suci Murdianto
Keyword(s):  
Medicinal Plants ◽  
Aloe Vera ◽  
Dry Weight ◽  
Fertilizer Application ◽  
Andrographis Paniculata ◽  
Randomized Design ◽  
Wet Weight ◽  
Growth Production ◽  
Completely Randomized Design ◽  
Total Dry Weight

Tanaman sambiloto (Andrographis paniculata Nees.) merupakan salah satu tanaman obat unggulan Indonesia selain temulawak, mengkudu, pegagan, lidah buaya, lada, dan kunyit. Peningkatan produktivitas sambiloto dapat dilakukan dengan perbaikan teknik budidaya melalui sistem pemupukan.  Kajian aplikasi pupuk guano terhadap pertumbuhan, produksi dan kandungan andgrographolida pada tanaman sambiloto terbatas, sehingga perlu dilakukan untuk mengetahui pengaruh pupuk guano terhadap pertumbuhan dan kandungan andrographolida pada tanaman sambiloto. Penelitian ini dilaksanakan di kebun percobaan Program Studi Agroteknologi Fakultas Pertanian Universitas Trunojoyo Madura pada bulan Januari - April 2016. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial dengan perlakuan 6 taraf dosis pupuk guano dengan 4 ulangan. Perlakuan dosis pupuk guano per hektar meliputi G0 (control/tanpa pupuk guano), G1 (5 ton), G2 (7.5 ton), G3 (10 ton), G4 (12.5 ton), dan G5 (15 ton). Pada setiap percobaan terdapat 6 tanaman dengan rincian 3 tanaman sebagai sampel. Hasil penelitian menunjukan bahwa pemupukan guano pada dosis 15 ton/ha (G5) memberikan hasil terbaik untuk parameter tinggi tanaman, jumlah daun, luas daun, berat basah total dan berat kering total tanaman. Kandungan andrographolida (%) tertinggi diperoleh pada dosis pupuk guano 7.5 ton/ha (G2), sedangkan produksi andrograpolida (mg) tertinggi ditunjukkan oleh perlakuan G4 (dosis pupuk guano 12.5 ton/ha).Response of Growth and Production of Srographic Andrographolida Plants (Andrographis paniculata Nees) Due to Differences in Guano Fertilizer Doses Bitter plant (Andrographis paniculata Nees.) including one featured Indonesian medicinal plants in addition to ginger, noni, gotu kola, aloe vera, pepper, and turmeric. Increased productivity is bitter to do with the improvement of farming techniques through a system of fertilization. Study of guano fertilizer application on the growth, production, and content andrographolide the bitter plant is limited, so it is necessary to know the effect of guano fertilizer on the growth and content of the plant andrographolide bitter. This research was conducted at the experiment station Agrotechnology, Faculty of Agriculture, University Trunojoyo Madura at January-April 2016 by using a completely randomized design (RAL) non-factorial with 4 replications consisting of G0 (control/without guano fertilizer), G1 ( fertilizer guano 5 tons/ha) G2 (7.5 tons/ha), G3 (10 tons/ha), G4 (12.5 tons/ha) and G5 (15 tons/ha) on each trial there are 6 plants with details of 3 plants in the sample. The results showed that the guano fertilizer at a dose of 15 tons/ha (G5) can be increased plant height, leaf number, leaf area, total wet weight, and total dry weight. The highest content of andrographolide obtained at doses of 7.5 tons/ha (G2) of guano fertilizer, while the highest yield of andrographolide obtained at doses 12.5 tons/ha of guano fertilizer (G4).

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