scholarly journals UJI KONSENTRASI CENDAWAN Beauveria bassiana (Bals.) Vuill TERHADAP MORTALITAS KEPIK POLONG (Riptortus linearis) F. PADA TANAMAN KEDELAI

2020 ◽  
Vol 22 (1) ◽  
pp. 9-15
Author(s):  
Rika Aprilia Fajar Riningrum ◽  
Nadrawati Nadrawati ◽  
Edhie Turmudi

[TESTING THE CONCENTRATION OF FUNGI Beauveria bassiana (Bals.) ON MORTALITY OF POD BORER (Riptortus linearis F.) ON SOYBEAN]. To control pod borer (Riptortus linearis F). on soybean using chemical insecticides can cause pollution to the environment, kill non-targeted insects and evolve pest resistance. The use of entomopathogenic fungus is an environmentally friendly alternative, one of which is Beauveria bassiana. (Bals.). The purpose of this study was to obtain an effective concentration of Beauveria bassiana fungi that can kill the pod borer at the 50% level.  The study was arranged in a Completely Randomized Design (CRD) consisting of 6 levels of fungi and 3 replications, where each experimental unit used 2 polybags.  Each treatment unit used 10 pod borers. The concentration of B. bassiana consisted of 105, 106, 107, 108 and 109 conidia / mL, and without fungi application as a control. The results indicated that the treatment of B. bassiana concentration on R. linearis significantly affected the percentage of mortality and the number of infected R. linearis, but no significant effect on the time of death, number of pods/plants and percentage of the number of damaged soybean pods. The mortality of R. linearis is classified as low, that is, less than 50%.

2012 ◽  
Vol 12 (1) ◽  
pp. 64-70
Author(s):  
Ni Siluh Putu Nuryanti ◽  
Lestari Wibowo ◽  
Abdul Azis

 A research was conducted to increase the virulence of Beauvaria bassiana to the rice bug, L.  acuta, by adding four different substances (materials) to the rice media to grow the fungus. The research was conducted from April to August 2011 at Polinela Laboratory, Bandar Lampung.  To do this a set of experimental unit consisting of five treatments and five replications were arranged in completely randomized design. The  four treatments were media made up of rice (rice based media) each given or added with four different materials such as rice skin powder, corn sugar, grasshopper powder and chitosan powder. The result showed that B. bassiana grown on rice media added with grasshopper powder or rice skin powder were able of causing mortality to the L. acuta as high as 78% and 71%, respectively, meaning that both mortalities were much higher than that of other replication. The virulence values of Beauveria bassiana  grown on those both powder were also higher than that of three others.


Author(s):  
Katherine D Vande Pol ◽  
Andres F Tolosa ◽  
Caleb M Shull ◽  
Catherine B Brown ◽  
Stephan A S Alencar ◽  
...  

Abstract Piglets experience a decline in body temperature immediately after birth, and both drying and warming piglets at birth reduces this. However, these interventions may have less effective at higher farrowing room temperatures. This study was carried out at a commercial facility to compare the effect of drying and/or warming piglets at birth on postnatal rectal temperature (RT) under relatively warm farrowing room temperatures (26.6 ± 2.09°C). Forty-five sows/litters were used in a completely randomized design to compare three Intervention Treatments (applied at birth): Control (no treatment); Warming (piglets placed in a plastic box under a heat lamp for 30 min); Drying+Warming (piglets dried with desiccant and warmed as above). Temperatures in the warming boxes over the study period averaged 37.7 ± 2.75°C. At birth, piglets were weighed; RT temperature was measured at 0, 10, 20, 30, 45, 60, 120, and 1440 min after birth. Blood samples were collected at 24 h after birth from a subsample of one piglet from each birth weight quartile within each litter to measure plasma immunocrit concentration. Data were analyzed using PROC MIXED of SAS with litter as the experimental unit; and piglet a subsample of litter. The model for analysis of piglet rectal temperature included fixed effects of treatment, measurement time (repeated measure), the interaction, and the random effect of sow. Compared to the Control, piglet RT were higher (P ≤ 0.05) for the Warming treatment between 10 and 60 min, and higher (P ≤ 0.05) for the Drying+Warming treatment between 10 and 120 min after birth. Rectal temperatures were higher (P ≤ 0.05) for the Drying+Warming than the Warming treatment between 20 and 120 min. Responses to drying and/or warming were greater for low birth weight piglets (< 1.0 kg) than heavier littermates, but were generally less than observed in previous experiments with similar treatments carried out under cooler temperatures. Piglet immunocrit values were lower (P ≤ 0.05) for the Drying+Warming treatment compared to the other treatments, which were similar (P > 0.05). Immunocrit values tended (P = 0.10) to be lower for light (< 1.0 kg) compared to heavier birth weight piglets. In conclusion, drying and warming piglets at birth was more effective for reducing piglet RT decline after birth than warming alone, though the effect was less than observed in previous studies carried out under cooler farrowing room temperatures.


2013 ◽  
Vol 14 (2) ◽  
pp. 308-321
Author(s):  
Lina Maria Peñuela Sierra ◽  
Ivan Moreira ◽  
Antonio Claudio Furlan ◽  
Paulo Levi de Oliveira Carvalho ◽  
Ângela Rocio Poveda Parra ◽  
...  

Two experiments were carried out to determine the bioavailability of phosphorus in two spray-dried yeasts - sugarcane yeast (SCY), and sugarcane yeast + brewer's yeast (SCBY) - in starting pigs, by comparing different methods (Apparent Digestibility Coefficient of Phosphorus - ADCP; True Digestibility Coefficient of Phosphorus - TDCP; slope ratio; and standard curve). In experiment I, a digestibility assay were carried out using 30 cross breed pig with initial weigh of 22.69 ± 4.24kg, allotted in a completely randomized design. The mean ADCP and TDCP values were 62.68 and 64.15% for SCY and 77.01 and 79.33% for SCBY. ADCP and TDCP for SCBY were higher (P<0.05) than the values for SCY. In Experiment II, a growth test was conducted, 56 crossbred piglets, were utilized, with initial live weight of 15.11 ± 3.43kg, allotted in a completely randomized design, with seven treatments, four replications, and two pigs per experimental unit. The treatments consisted of a basal diet without supplementation with P and the same diet including supplementation with two levels of P (0.053% and 0.105%) from dicalcium phosphate, SCY and SCBY. The relative bioavailability mean value of 57.23% for SCY and 91.96% for SCBY, corresponding to 0.30% and 0.40% of available phosphorus, respectively.


Revista CERES ◽  
2011 ◽  
Vol 58 (5) ◽  
pp. 645-650 ◽  
Author(s):  
Gilberto Bernardo de Freitas ◽  
Márcio Sousa Rocha ◽  
Ricardo Henrique Silva Santos ◽  
Letícia Monteiro da Silva Freitas ◽  
Leandro de Almeida Resende

The objective of this work was to evaluate the productive performance of broccoli under different top-dressing organic fertilizations. The experiment was conducted under protected cultivation, in a completely randomized design with four replications, with two plants per experimental unit. Broccoli seedlings were produced in a commercial substrate in styrofoam trays. The seedlings were transplanted to plastic pots containing 10.0 L of substrate made up of subsoil and organic compost at the ratio of 3:1 (v/v), respectively, which is equivalent to about 20.0 t ha-1 of organic compost at planting. After seedling establishment, the top-dressing fertilization treatments were applied: gliricidia biomass associated or not with liquid biofertilizer of cattle manure to the soil and bokashi. Two control treatments were established: one with mineral fertilization recommended for the crop and the other without top-dressing fertilization. The broccoli production was evaluated (commercial standard). Plants that received mineral fertilizer were more productive, however, they were not significantly different (p>0.05), by Dunnet test, from the plants fertilized with 2.5 t ha-1 gliricidiabiomass (dry mass) associated with liquid biofertilizer (2.0 L m-2) applied to soil. Top-dressing fertilizations with only gliricidia, at 2.5 and 5.0 t ha-1 of biomass (dry mass), resulted in no significant increase in production of broccoli inflorescence. The use of bokashi in addition to gliricidia biomass and liquid biofertilizer reduced the efficiency of the fertilization compared with plants that received only gliricidia and liquid biofertilizer.


Author(s):  
Sri Sukamto

One of the entomopatogenous fungus types commonly observed and showed potency as myco-insectiside is Beauveria bassiana(Bals.) Vuill.In order to support effectiveness and patogenous activity of B. bassiana, it is necessary to add a carrying agent that protects its spores from ultra violet ray. This study aims to investigate the effect of storage temperature on viability of B. Bassianaspores on the carrier material. The observation was carried out in the Laboratory of Plant Diseases, Indonesian Coffee and Cocoa Research Institute. The research was arranged in completely randomized design by three factors. The first factor was carrier (C), that consists of C1 = rice flour, C2 = maize flour and C3 = tapioca flour. The second factor was dosage (D), that consists of D1 = 1 g B. bassiana+ 1 g carrier; D2 = 1 g B. bassiana+ 5 g carrier and D3 = 1 g B. bassiana+ 10 g carrier. The third factor was temperature of the storage (T),that consists of T1 = 5oC; T2 = 23oC and T3 = 29oC. Viability of B. Bassiana spores was examined by observing development of 100 blastopores randomly and determined under light microscope with 400 times magnification. Observation was conducted in two replicates after the spores of B. bassiana were kept in the storage for 2, 4, 8 and 16 weeks. The result showed that by adding 1 g tapioca flour and temperature of storage of 5oC was potentiall in keeping viability of B. bassianaspores at least for 2 months. It was due to that tapioca flour gave better effect than rice and maize flours in keeping the storage and appropriate low temperature. Viability of B. bassianaspores decreased with increasing carrier dosage, temperature and duration of the storage. Whereas, storage at 5oC was found to be a better condition in keeping viability of dry pure B. bassianaspores longer than conditions of 23o and 29oC. Key word:Beauveria bassiana, temperature, viability,carrier.


2012 ◽  
Vol 14 (1) ◽  
pp. 126
Author(s):  
Yekki Yasmin ◽  
Lenni Fitri ◽  
Betty Mauliya Bustam

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.


2020 ◽  
Vol 1 (1) ◽  
pp. 1-6
Author(s):  
Mihwan Sataral ◽  
Hendra Heri Robika ◽  
Zaedar A Masese

Salah satu faktor yang menyebabkan penurunan produksi kakao yaitu serangan hama penggerek buah kakao. Semut hitam (Dolichoderus thoracicus) diketahui sebagai agen hayati pengendali hama penggerek buah kakao. Penelitian ini bertujuan untuk menghitung kemampuan memangsa semut hitam pada larva penggerek buah kakao, dan menghitung persentase larva penggerek buah kakao yang dimangsa serta mengukur potensi semut hitam sebagai agen hayati penggerek buah kakao. Penelitian ini di laksanakan di Desa Jaya Makmur Kecamatan Nuhon Kabupaten Banggai. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 3 ulangan, dengan masing-masing perlakuan sebagai berikut : P1 (20 larva:10 predator), P2 (20 larva:15 predator), P3 (20 larva:20 predator), P4 (20 larva:25 predator), P5 (20 larva:30 predator), P6 (20 larva:35 predator). Hasil penelitian menunjukkan bahwa perlakuan semut hitam pada larva penggerek buah kakaoSalah satu faktor yang menyebabkan penurunan produksi kakao yaitu serangan hama penggerek buah kakao. Semut hitam (Dolichoderus thoracicus) diketahui sebagai agen hayati pengendali hama penggerek buah kakao. Penelitian ini bertujuan untuk menghitung kemampuan memangsa semut hitam pada larva penggerek buah kakao, dan menghitung persentase larva penggerek buah kakao yang dimangsa serta mengukur potensi semut hitam sebagai agen hayati penggerek buah kakao. Penelitian ini di laksanakan di Desa Jaya Makmur Kecamatan Nuhon Kabupaten Banggai. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 3 ulangan, dengan masing-masing perlakuan sebagai berikut : P1 (20 larva:10 predator), P2 (20 larva:15 predator), P3 (20 larva:20 predator), P4 (20 larva:25 predator), P5 (20 larva:30 predator), P6 (20 larva:35 predator). Hasil penelitian menunjukkan bahwa perlakuan semut hitam pada larva penggerek buah kakao (Conopomorpha cramerella) berpengaruh sangat nyata terhadap larva penggerek buah kakao. Jumlah larva penggerek yang dimangsa tertinggi pada perlakuan P6 (20 larva:35 predator) dua minggu setelah aplikasi dengan nilai rata-rata 16,3 kemudian satu minggu setelah aplikasi dengan nilai rata-rata 14,3. Persentase jumlah larva penggerek yang dimangsa tertinggi pada perlakuan P6 yaitu 81% pada saat dua minggu setelah aplikasi dan 0,71% pada saat satu minggu setelah aplikasi. (Conopomorpha cramerella) berpengaruh sangat nyata terhadap larva penggerek buah kakao. Jumlah larva penggerek yang dimangsa tertinggi pada perlakuan P6 (20 larva:35 predator) dua minggu setelah aplikasi dengan nilai rata-rata 16,3 kemudian satu minggu setelah aplikasi dengan nilai rata-rata 14,3. Persentase jumlah larva penggerek yang dimangsa tertinggi pada perlakuan P6 yaitu 81% pada saat dua minggu setelah aplikasi dan 0,71% pada saat satu minggu setelah aplikasi. One of the factors that caused the decline in cocoa production was the attack of cocoa pod borer. Black ants (Dolichoderus thoracicus) are known to be biological agents to control cocoa pod borer. This study aims to calculated the ability to prey on black ants in the cocoa pod borer larvae, the percentage of cocoa pod borer larvae that are eaten and to measured the potential of black ants as biological agents of cocoa pod borer. This research was conducted in Jaya Makmur Village, Nuhon District, Banggai Regency. The research used a completely randomized design consisted of 6 treatments and 3 replications, with each treatment as follows : P1 (20 larvae: 10 predators), P2 (20 larvae: 15 predators), P3 (20 larvae: 20 predators), P4 (20 larvae: 25 predators), P5 (20 larvae: 30 predators), and P6 (20 larvae : 35 predators). The results showed that the treatment of black ants on cocoa pod borer larvae (Conopomorpha cramerella) had a very significant effected on the cocoa pod borer larvae. The highest number of dead borer larvae was in the P6 treatment (20 larvae: 35 predators) two weeks after application with an average value of 16.3 then one week after application with an average value of 14.3. The highest percentage of borer larvae preyed on the P6 treatment was 81% at two weeks after application and 0.71% at one week after application.


2014 ◽  
Vol 15 (1) ◽  
pp. 221-236 ◽  
Author(s):  
Liliane Maria Piano Gonçalves ◽  
Ivan Moreira ◽  
Paulo Cesar Pozza ◽  
Paulo Levi de Oliveira Carvalho ◽  
Juliana Beatriz Toledo ◽  
...  

Two experiments were carried out to determine the nutritional value and to evaluate the performance and carcass traits in the growing-finishing pigs fed on two types of semipurified glycerin, which were made from vegetable oil (SPGV) and mixed of animal + vegetable oil (SPGM). In the digestibility trial (experiment I) 32 crossbreed barrows were used with initial body weight of 45.08 ± 4.11kg. It were used three levels of inclusion (6, 12 e 18%) of semipurified glycerin (SPGV and SPGM) in the basal diet. The metabolizable energy (ME) value of glycerin were estimated by regression of ME (kcal/kg) intake associated with glycerin vs. glycerin intake (kg). The values of ME (kcal/kg) obtained were: 2,731 for SPGV and 2,210 for SPGM. In experiment II, 72 pigs (30.08 ± 1.65 to 60.58 ± 3.57) in growing and (60.83 ± 2.63 to 93.79 ± 5.72) in finishing phase, were allotted in a completely randomized design in a 2 x 4 + 1 factorial scheme, with two types of semipurified glycerin (SPGV and SPGA), four inclusion levels (4; 8; 12 and 16%), eight experimental unit and a control diet containing no glycerin (0%). All pigs were slaughtered to evaluate the carcass traits. The performance results suggest that it is feasible to use up to 16% of both semipurified glycerins on growing and finishing pigs feeding, without impairing performance and carcass traits.


2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Eka Irma Q Bariah ◽  
Siegfried Berhimpon ◽  
Eunike Louisje Mongi

Edible coating is a new type of packaging made of biopolymer. The advantages of edible coating is enable to protect the food product, the original appearance of the product can be maintained and the product can be eaten as well as environmentally friendly. The study aim was to look at the organoleptic characteristics of fish cake coated with edible coating of carrageenan were given a liquid smoke. The treatments  given were variation of carrageenan concentration i,e. 0%, 1%, 1,5%, 2%, 2,5%, 3%. The experiment was designed using a Completely Randomized Design (CRD). To determine the effect of treatments, the data were tested using organoleptical tests consists of hedonic and texture.The results shown that hedonic test of the appearance and color of the raw sample have significant effect (p>0,05), while the other test showed no significant effect (p>0,05). However, the organoleptical score for taste, smell and texture shown a tendency that the fish cake coated, have  higher score than without coated. Keywords: Edible coating, Carrageenan, Fish cake, Liquid smoke.


2019 ◽  
Vol 49 (1) ◽  
Author(s):  
Maria Cristina Manno ◽  
Luiz Fernando de Souza Rodrigues ◽  
Kedson Raul de Souza Lima ◽  
Monique Valéria de Lima Carvalhal ◽  
Silas Santiago Rodrigues Filho ◽  
...  

ABSTRACT: This study aimed to evaluate behavioral aspects of Santa Inês sheep in pasture in humid tropical climate. In order to do so, nine Santa Inês sheep were kept on pasture, exposed to the climate directly, without shade, for 24 days, with 12 alternate days in each period (dry and raining) in 2015. The behavioral parameters evaluated were: food consumption, standing idleness, lying idle and rumination. Observations occurred from 08:00 A.M. to 05:55 P.M. The experimental was a completely randomized design (CRD) with two treatments (rainy season and dry period), nine replications, one animal per experimental unit. To understand the correlations of the behavioral parameters with the climatic variables, Pearson’s correlation was performed. Approximate average values between the rainy and dry periods for the behavioral variables were observed, but there was a greater intake in the volume of water in the dry period (9.59L/sheep). Also, there were significant differences in the behavior of the animals throughout the day. In the hottest time of the day, at 02:00 P.M., there was standing idle of 25.83min., lying idle of 1.21min., and spent less time-consuming food (27.08min.) in relation to other schedules. We concluded that the sheep had similar behavioral parameters in the rainy and dry periods, but when we observe the times of the days, the behaviors are different. The main activity recorded was grazing, and the variables, temperature and relative humidity of the air influence differently between the seasons, being more marked in the dry period.


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