scholarly journals Effect of Adding Cassava Peel and Lactic Acid Bacteria as a Feed Additive to the Weight of Immune Organs of Super Native Chicken

2021 ◽  
Vol 16 (4) ◽  
pp. 334-339
Author(s):  
P. Ardiansyah ◽  
E. Suprijatna ◽  
S. Kismiati

This study aims to evaluate the use of cassava peels added with lactic acid bacteria on the immune organ weight of super native chickens. The material used in this study was 144 Day Old Chick (DOC) super unsex native chicken with an average body weight of 34 ± 3,14 gram. The research design used was a completely randomized design (CRD) with a dose of liquid feed additive in the form of a combination of cassava peel flour and lactic acid bacteria mixed with rations. The treatments in this study included T0: basal ration, T1 (basal ration + 10% feed additive ml/kg), T2 (basal ration + 15% feed additive ml/kg) and T3 (basal ration + 20% feed additive ml/kg). The parameters observed were body weight, the relative weight of lymph, thymus, and bursa Fabricius superficial chicken stock exchange. The result showed that the combination of cassava peel and lactic acid bacteria had no significant effect (P>0.05) on body weight, the relative weight of the lymph organs, thymus, and bursa Fabricius of super native chickens. This research concludes that the combination of cassava peel and lactic acid bacteria as a feed additive can maintain chickens' health by not increasing the relative weight of the super native chicken immune organs to the level of 200 ml/kg.

2021 ◽  
Vol 21 (2) ◽  
pp. 157-164
Author(s):  
Lilik Krismiyanto ◽  
Nyoman Suthama ◽  
Hanny Indrat Wahyuni

ABSTRACT. Penelitian bertujuan untuk mengkaji perkembangan bakteri usus halus dan performan ayam kampung silangan kampung-leghorn (KL) akibat ditambahkan ekstrak umbi dahlia dalam ransum. Ternak percobaan yang digunakan adalah persilangan ayam kampung silangan KL sebanyak 200 ekor umur 22 hari dengan rata-rata bobot badan 180,46 ± 1,21 g. Bahan perlakuan meliputi umbi dahlia, ethanol 70%, kertas saring halus serta bahan pakan penyusun ransum (jagung giling, bekatul, bungkil kedelai, tepung ikan, CaCO3 dan mineral dan vitamin mix). Rancangan percobaan yang digunakan rancangan acak lengkap dengan 4 perlakuan dan 5 ulangan (masing-masing unit percobaan diisi 10 ekor). Perlakuan yang diterapkan meliputi: P0=Ransum kontrol/RK, P1=RK+0,39% ekstrak umbi dahlia, P2=RK+0,78 ekstrak umbi dahlia, dan P3= RK+1,17 ekstrak umbi dahlia. Parameter yang diukur meliputi populasi bakteri asam laktat, Escherichia coli, pH digesta tiap masing-masing segmen usus halus dan pertambahan bobot badan harian (PBBH). Data dianalisis dengan menggunakan uji sidik ragam dan jika berpengaruh nyata dilakukan uji beda nyata Duncan pada taraf 5%. Hasil penelitian menunjukkan bahwa penambahan ekstrak umbi dahlia berpengaruh nyata terhadap populasi bakteri asam laktat, Escherichia coli, pH digesta tiap masing-masing segmen usus halus dan PBBH. Kesimpulan adalah penambahan ekstrak umbi dahlia sampai taraf 1,17% sebagai sumber inulin di dalam ransum dapat meningkatkan populasi bakteri asam laktat, menurunkan pH dan Escherichia coli pada masing-masing segmen usus halus serta memperlambat laju digesta dan meningkatkan PBBH.  (Population of small intestine bacteria and performance of native chicken-leghorn crossbreed duo to feeding of dahlia tuber extract) ABSTRAK. This study aims to examine the development of small intestine bacteria and the performances of native chicken-leghorn crossbreed due to the addition of dahlia tuber extract in the diet. Experimental animals were 200 unsex native chicken-leghorn crossbreed, 22 days old with an average body weight of 180.46 ± 1.21 g. Treatment materials include dahlia tuber, ethanol 70%, fine filter paper and feed stuff (yellow corn, rice bran, soy bean meal, fish meal, CaCO3, and vitamin-mineral mix). The present experiment was assigned in a completely randomized with 4 treatments and 5 replications (10 birds each). The treatments were: P0=Control Diet/CD, P1=CD+0,39% dahlia tuber extract, P2=CD+0,78% dahlia tuber extract and P3=CD+1,17% dahlia tuber extract. Parameters measured were the number of Lactic Acid Bacteria (LAB), Escherichia coli, intestinal potential hydrogen (pH) (duodenal, jejunal and ileal), rate of passage and daily body weight gain (DBWG). Data were subjected to ANOVA and followed by Duncan Multiple Range Test on 5% level. The results indicated that feeding native chicken-leghorn crossbreed with the diet containing dahlia tuber extract (DTE) significantly (P0.05) increased LAB population and DBWG, decreased E. coli population and intestinal pH, and slow the rate of passage down. In conclusion, feeding of dahlia tubers as a source of inulin in the form of dahlia tuber extract to a level of 1.17% increased the population of lactic acid bacteria, reduced pH and Escherichia coli in each segment of the small intestine and retard the rate of digestion and increased body weight gain.


2018 ◽  
Vol 62 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Marta Kupryś-Caruk ◽  
Monika Michalczuk ◽  
Beata Chabłowska ◽  
Ilona Stefańska ◽  
Danuta Kotyrba ◽  
...  

AbstractIntroductionOne aim of the study was to evaluate the impact when added to feed of the two potentially probiotic strains of lactic acid bacteria (LAB) Lactobacillus plantarum K KKP 593/p and Lactobacillus rhamnosus KKP 825 on production performance, health, and the composition of gut microbiota. The complementary aim was to assess the safety of these strains in broiler rearing.Material and MethodsA total of 500 one-day-old Ross 308 chicks were divided into four groups. The experimental factor was the admixture of bacterial preparation to the feed at different doses: the recommended maximum dose, a dose ten times higher, the recommended minimum dose, and a zero dose for the control group not receiving bacteria.ResultsAddition of bacteria to the diets did not have a significant effect on the final body weight, final body weight gain, nor total feed intake or feed conversion. However, lactic acid bacteria had a positive effect on chicken health. Mortality among chickens fed with LAB was reduced. Moreover, LAB feeding inhibited the growth of Salmonella spp. and Clostridium perfringens in the intestines. There were no significant differences in chicken performance by dose of bacteria in the feed. The group dosed with LAB ten times higher than the recommended maximum did not demonstrate changes in biochemical or haematological parameters of blood compared to the remaining groups.ConclusionFeeding chicken broilers with two potentially probiotic LAB strains is safe and impacts animal health positively.


2015 ◽  
Vol 6 (4) ◽  
pp. 505-512 ◽  
Author(s):  
M. Yakovlieva ◽  
T. Tacheva ◽  
S. Mihaylova ◽  
R. Tropcheva ◽  
K. Trifonova ◽  
...  

In recent years, many authors have investigated the possible antidiabetic effect of lactic acid bacteria. Lactobacillus species constitute a major part of the lactic acid bacteria group and have been found to exhibit beneficial effects on the development of diabetes and its complications. In the current study, we investigated the effects of newly characterised Bulgarian Lactobacillus strains, Lactobacillus brevis 15 and Lactobacillus plantarum 13, on blood glucose levels and body weight of rats fed a fructose-enriched diet. An experiment was conducted over a period of 8 weeks with 24 2-month-old Wistar rats randomly assigned to receive a standard diet (Con, control group), fructose-enriched diet (Fr group), standard diet with probiotics given twice a week (Pro group), and fructose-enriched diet with probiotics given twice a week (Pro+Fr group). At the end of the experimental period, a statistically significant increase in body weight was observed in all experimental groups (P<0.0001). The highest rise was seen in the fructose group (Fr, 169±19 g), followed by the Pro+Fr group (153±15 g), Pro group (149±13 g), and Con group (141±5 g). Moreover, the final blood glucose levels had risen significantly in the groups receiving fructose either without (Fr; P<0.0001) or with lactobacilli (Pro+Fr; P=0.002), while the rise was insignificant in the group of rats given probiotic supplementation only (Pro, P=0.071) and inexistent in the Con group (P=0.999). The highest elevation of blood glucose levels was observed in the Fr group (3.18 mmol/l), followed by the Pro+Fr group (2.00 mmol/l) whereas the Pro group showed the lowest levels (0.60 mmol/l). The results of our study suggest that the newly characterised Bulgarian Lactobacillus strains, L. brevis 15 and L. plantarum 13, could be considered as possible probiotics and might be able to prevent some metabolic disturbances.


2017 ◽  
Vol 13 (2) ◽  
pp. 69
Author(s):  
A. F. Fanani ◽  
N. Suthama ◽  
B. Sukamto

The research was aimed to evaluate used dahlia tubersextract as inulinsource onnitrogen retention and feed conversion of crossbred local chicken (male native chicken × female laying hen). Three weeks old 160 unsex birds with average body weight 180.46±1.21g were kept until 8 weeks. The experimentwas designed ascompletely randomized design with 4 treatments and 4 replications (@10 birds). Treatments applied were T0: basal ration, T1: ration + 0.39% extract, T2: ration + 0.78% extract, T3: ration + 1.17% extract. Parameters observed werenitrogen retention, energy metabolism and feed conversion. The data were statistically analyzed according to ANOVA and continued by Duncan test at the level of 5%. The results showed that feeding dahlia tuberextract significantly affect (P&lt;0.05) nitrogen retentionwith value T0-T3 (1.3g, 1.47g, 1.43g, 1.7g)and feed conversion (4.15, 3.58, 3.75, 3.47), but not significant of energy metabolism. In conclusion, the higher levels of feeding inulin in the form of extract in T3 (1.17%), increase nitrogen retention and improve feed conversionof crossbred local chicken.


2007 ◽  
Vol 2 (1) ◽  
pp. 22
Author(s):  
Enok Sobariah ◽  
Ali Khomsan ◽  
Ingrid S. Surono

<p class="MsoNormal" style="margin: 0cm 12.45pt 6pt 17.85pt; text-align: justify;"><span style="font-size: 10pt;" lang="en-us" xml:lang="en-us">The aim of this study were  to identify the in-vitro tolerance of pro-biotic bacteria to acid and bile salt condition; and  to prove a hypothesis that the supplementation of oxygenated water has a positive effect on the body weight of rat and on viability of pro-biotic bacteria.  The first study was carried out at PAU Laboratory of Bogor Agricultural University, while the second study was conducted at Department of Community Nutrition of Bogor Agricultural University and Microbiology Laboratory of Indonesia Institute of Technology. Forty five rats aged 6 weeks were divided into three groups, i.e., control group without probiotic (a0), Lactobacillus casei Shirota (a1), and Lactobacillus IS-7257 (a2).  Each group (consisting of 5 rats each) has three different treatments, namely, control without oxygenated water (b0), 50 ppm oxygenated water (b2), and 80 ppm oxygenated water (b2). Oxygenated water was administered to the rats twice a day in the morning (3.25 ml) and afternoon (3.00 ml). Observation was carried out on the body  weight of the rats, fecal lactic acid bacteria, coliform, and anaerob bacteria by plate counting, for 4 periods, i.e, prior to the treatment (C0), after three-day treatment (C1), after seven-day treatment (C2), and on the 10<sup>th</sup> day treatment or three days after washed out period. The results indicated that probiotic bacteria are resistant to acid and bile acid condition. Oxygen concentration in water has a significant positive influence on the body weight of rats towards viability of probiotic bacteria (p-level &lt; 0.05).  The supplementation of  oxygenated water 50 ppm significantly increase the population of viable fecal lactic acid bacteria in L. casei Shirota and Lactobacillus IS-7257 groups after 3 and 7 days of treatment.  Lactobacillus IS-7257 gave better response than L. casei Shirota. The supplementation of oxygenated water 80 ppm significantly reduces the fecal coliform in-vivo in both L. casei Shirota and Lactobacillus IS-7257 groups (p-level &lt; 0.05).</span></p>


2020 ◽  
Vol 4 (2) ◽  
pp. 54-58
Author(s):  
H. Husnaeni ◽  
J. Junaedi ◽  
Wahyu Ningsi

This study was aimed to determine the effect of providing a combination of tofu dregs fermentation with commercial feed on the growth of super native chickens. The research was being conducted in June-September 2019 at the Integrated Laboratory of the Faculty of Agriculture, Fisheries and Animal Husbandry, University of Sembilanbelas November Kolaka. The materials were used in this study were super native chicken, fermented feed, and commercial feed (Malindo). The fermented feed consists of tofu dregs, yellow corn, fine rice bran, starch, molasses. Tools that used were digital scales, pans, tarpaulins, cages, feed containers, drinking containers, cables, lamps, sacks, plastic polybags, sacks, feed grinding machines, gas, hoses, buckets, basins. This research was conducted using a Completely Randomized Design (CRD) 4 treatments three replications with each test consisting of 8 super native chickens. The parameters observed were Weekly Consumption, Weekly Body Weight Gain (WBWG), and Feed Conversion. The results showed that the influence of feed consumption was not significant (P> 0.05), while the WBWG and Feed Conversion were significantly different due to treatment. So it can be concluded that the use of a combination of fermented tofu pulp and commercial feed with a ratio of 60% and 40% can increase weekly body weight gain and decrease feed conversion so that the use of feed is more efficient


2021 ◽  
Vol 204 (1) ◽  
Author(s):  
Katarzyna Marchwińska ◽  
Daniela Gwiazdowska

AbstractAnimal microbiota is becoming an object of interest as a source of beneficial bacteria for commercial use. Moreover, the escalating problem of bacterial resistance to antibiotics is threatening animals and humans; therefore, in the last decade intensive search for alternative antimicrobials has been observed. In this study, lactic acid bacteria (LAB) were isolated from suckling and weaned pigs feces (376) and characterized to determine their functional properties and usability as pigs additives. Selection of the most promising LAB was made after each stage of research. Isolates were tested for their antimicrobial activity (376) and susceptibility to antibiotics (71). Selected LAB isolates (41) were tested for the production of organic acids, enzymatic activity, cell surface hydrophobicity and survival in gastrointestinal tract. Isolates selected for feed additive (5) were identified by MALDI-TOF mass spectrometry and partial sequence analysis of 16S rRNA gene, represented by Lentilactobacillus, Lacticaseibacillus (both previously classified as Lactobacillus) and Pediococcus genus. Feed additive prototype demonstrated high viability after lyophilization and during storage at 4 °C and − 20 °C for 30 days. Finally, feed additive was tested for survival in simulated alimentary tract of pigs, showing viability at the sufficient level to colonize the host. Studies are focused on obtaining beneficial strains of LAB with probiotic properties for pigs feed additive.


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