scholarly journals Screening and characterization of potential bioactive compounds from Selaginella wightii

2020 ◽  
Author(s):  
Abheepsa Mishra
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Fungal Infections ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Ether Extract ◽  
Diethyl Ether Extract ◽  
Dpph Method

A chemical investigation of the whole plant of Selaginella wightii was undertaken and the extracts of Selaginella wightii were screened for antioxidant and antimicrobial activities . The antioxidant activity by DPPH method was higher in ethanol extract and diethyl ether extract. In β-carotene-Linoleic acid assay, ethanol extract and diethyl ether extract showed good inhibition zone exhibiting significant antioxidant activity. The various extracts were then examined for antimicrobial activity by using the standard well-diffusion method. The diethyl ether extracts, ethanol, and aqueous extracts showed higher levels of antimicrobial activity than the other extracts. All the extracts also showed good anticandidal activity. The extracts were found to be rich in flavonoids and three compounds were identified. Our study indicates the potential use of Selaginella wightii extracts for treating antimicrobial or fungal infections.

scholarly journals Identifikasi Komposisi Kimia dan Uji Aktivitas Antioksidan dari Biji Kurma (Phoenix dactylifera)

2018 ◽  
Vol 4 (2) ◽  
pp. 182-189
Author(s):  
Yusraini Dian Inayati Siregar ◽  
Tarso Rudiana ◽  
Windi Riyadi
Keyword(s):  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Date Palm ◽  
Octadecenoic Acid ◽  
Ethanol Extract ◽  
Phoenix Dactylifera ◽  
Seed Extract ◽  
Hexane Extract ◽  
Ether Extract ◽  
Diethyl Ether Extract

Dates are a component of dates that have 6.10-11.47% of all dates. In some countries, dates are a major problem in the processing industry because the seeds currently only become waste. This research was conducted to determine the solvent that produces the largest yield using the soxhletation method and determine its antioxidant activity. Dates are disoxletasion with a variety of solvents n-hexane, diethyl ether, and ethanol. Also carried out the variation of extraction time for 1.5; 2; and 2.5 hours. Based on the results of the study showed that the results of the largest date seed extract were obtained using ethanol of 4.93% for 2.5 hours. Antioxidant activity of date palm seed extract (IC50) using DPPH ethanol extract method has IC50 of 2.27 ppm; n-hexane extract 8396.56 ppm; and diethyl ether extract 9826.88 ppm. Inhibition of date palm seed extract using the TBA method derived from ethanol extract of 98.22% at a concentration of 250 ppm. The results of the analysis using LC-MS showed that ethanol extract was thought to contain 13-hydroxyabscisic acid compound, 1-ethylidene-4-methylidene-2oxo-7-(propan-2-il)-tocahydro-1H-indent-5-il- 3-methylpent-2-enoate, 12-hydroxy-acid (8,10,14) -eicosatrienoic and 1-hydroxy-3- (pentadecanoiloxy) propan-2-il (5,8,11,14) -icosa-5, 8,11,14-tetraenoate. The results of the analysis using GC-MS on n-hexane extract showed 2,4-di-tert-butylphenol and 9-octadecenoic acid. Diethyl ether extract contains 9-octadecenoic acid and mono (2-ethylhexyl) benzocarboxylic acid.  

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

scholarly journals Chemical composition and screening of the antimicrobial and anti-oxidative activity of extracts of Stachys species

2010 ◽  
Vol 75 (10) ◽  
pp. 1347-1359 ◽  
Author(s):  
Jelena Lazarevic ◽  
Radosav Palic ◽  
Niko Radulovic ◽  
Novica Ristic ◽  
Gordana Stojanovic
Keyword(s):  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Salmonella Enteritidis ◽  
Balkan Peninsula ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Diethyl Ether Extract ◽  
The Common ◽  
Ethyl Acetate Extracts

GC and GC/MS analyses of the diethyl ether and ethyl acetate extracts obtained from the aerial parts of Stachys germanica subsp. heldreichii (Boiss) Hayek, Stachys iva Griseb., Stachys plumosa Griseb. and Stachys scardica Griseb., Balkan peninsula endemics, were performed. One hundred and seventy-nine constituents, accounting for 88.8-98.1% of the total composition of the extracts, were identified. The common feature of the diethyl ether extracts was the high content of terpenoids and fatty acidderived compounds, while the common feature of the ethyl acetate extracts was the prevalence of fatty acid-derived compounds. A disk diffusion method was used for the evaluation of the antimicrobial activities of the extracts against a panel of microorganisms (bacteria: Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Salmonella enteritidis; fungi: Aspergillus niger and Candida albicans). The total antioxidant capacity of the extracts was evaluated by the phosphomolybdenum method. The preliminary bioassay results indicated that the diethyl ether extract of S. plumosa could be a possible source of antioxidant and antimicrobial compounds.

scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF COCOA POD THAT ASSOCIATED IN MALTODEXTRIN IN VARIOUS CONCENTRATION

2018 ◽  
Vol 5 (2) ◽  
pp. 123
Author(s):  
Asriani Hasanuddin ◽  
Chairil Anwar ◽  
Marhawati Mappatoba ◽  
Hafsah Hafsah
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Diffusion Method ◽  
Natural Food ◽  
Skin Extract ◽  
E Coli ◽  
Dpph Method ◽  
Antioxidant And Antimicrobial Activity ◽  
Peel Extract

Cocoa pod extract ((Theobroma cacao L.) has antioxidant and antimicrobial activity that has the potential as a natural food preservative. However, in its use the cocoa fruit skin extract has a disadvantage because the short shelf time and its application to food are limited, efforts are needed to prevent damage and extend shelf life, one of the efforts that can be done is by encapsulating the extract.This study aims to determine the antibacterial activity and antioxidant encapsulation of cocoa peel extract, this study begins with the extraction of cocoa pods with ethanol solvent by comparing cocoa pods : solvent 1: 4 The skin of cacao cocoa fruit used is yellow harvested cocoa fruit, then chopped and dried to form flour.The sample is extracted by maceration with ethanol solvent Antioxidant test is done by DPPH method, while antibacterial test is carried out by the well diffusion method. This study used a completely randomized design method (CRD) with 5 treatments using a maltodextrin concentration of 20% (M1); 30% (M2); 40% (M3); 50% (M4) and 60% (M5). The results showed that the treatment gave the highest yield in the treatment of 60% maltodextrin concentration (M5), while the highest antioxidant activity was obtained in the treatment of 20% maltodextrin (M1) with IC50 75.98 µg / mL and the treatment with the lowest antioxidant activity was obtained at treatment of 60% maltodextrin concentration (M5) with IC50 value 114.89 µg / mL. While for the antimicrobial activity also obtained with the same results, namely treatment of 20% (M1) obtained a higher inhibition diameter compared to treatment at 30%; 40%; 50% and 60% for all types of bacteria. The inhibition diameter in the treatment of the concentration of maltodextrin 20% (M1) for E. coli bacteria is between 4.12 mm - 10.95 mm, Salmonella sp is 2.85 mm - 8 , 25 mm and for Staphylococcusaureus of 5.15 mm - 13.90 mm and the lowest inhibition diameter was obtained in the treatment of 60% maltodextrin concentration (M5) for E. coli bacteria of between 2.0 mm - 4.79 mm, Salmonella sp of 1.15 mm - 4.35 mm and for Staphylococcusaureusat 2.76 mm - 5.17 mm.This study concluded that the encapsulation of cocoa peel extract using 20% maltodextrin had the highest antioxidant and antimicrobial activity when compared with other treatments namely 30% concentration; 40%; 50% and 60% but for the treatment of 20% and 30% there is no difference. Ethanol extract of cocoa pods can be made in the form of encapsulates which are very likely to be used as natural preservatives.

Comparative study of the ethanol extracts of six Acinos Miller species: chemical composition, antimicrobial and antioxidative activities

2020 ◽  
Vol 48 (1) ◽  
pp. 53-65
Author(s):  
Tatjana D. GOLUBOVIĆ ◽  
Gordana S. STOJANOVIĆ ◽  
Dušanka V. KITIĆ ◽  
Bojan K. ZLATKOVIĆ ◽  
Dragana R. PAVLOVIĆ ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Salmonella Enteritidis ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Fungal Organisms ◽  
Ethanol Extracts

The ethanol extracts of selected Acinos Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of A. majoranifolius, A. suaveolens and A. alpinus were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the A. arvensis and A. graveolens extracts reached 28.97% and 30.75%. Also, n-alkanes were the major compounds found in A. hungaricus ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus; Gram-negative: Klebsiella pneumoniae, Pseudomonas aerugionsa, Salmonella enteritidis, Proteus vulgaris, Escherichia coli and fungal organisms: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae were used as test microorganisms. The results of preliminary bioassay demonstrated that the A. alpinus ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.

scholarly journals Antibacterial, anti-inflammatory and antioxidant activities of Mahanintangtong and its constituent herbs, a formula used in Thai traditional medicine for treating pharyngitis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Bhanuz Dechayont ◽  
Pathompong Phuaklee ◽  
Jitpisute Chunthorng-Orn ◽  
Thana Juckmeta ◽  
Onmanee Prajuabjinda ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Biological Activities ◽  
Ethanol Extract ◽  
Essential Medicines ◽  
Diffusion Method ◽  
Plant Materials ◽  
Tnf Α ◽  
Anti Inflammatory

Abstract Background Mahanintangtong is listed in the Thailand’s National List of Essential Medicines (NLEM). It is used to treat non-specific fevers and illnesses such as pharyngitis and chickenpox. In this study, we investigated the biological activities of the different medicinal plants used in the Mahanintangtong formula. Methods The plant materials were extracted by maceration and decoction. Antimicrobial activity, assessed by disc diffusion method, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were compared with commercially available standard antibiotics. To elucidate the anti-inflammatory mechanisms, inhibition of nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin 6 (IL-6) production was tested by Griess and ELISA techniques. Antioxidant activity was measured by ABTS and DPPH scavenging assays. Results The extracts with the best antimicrobial activities were carbonized Tectona grandis showing against Streptococcus pyogenes, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. The ethanol extract of Dracaena loureiroi wood exhibited the highest NO and IL-6 inhibitory activity with IC50 values of 9.42 ± 1.81 and 12.02 ± 0.30 μg/mL, respectively. The ethanol extract of Pogostemon cablin had the highest TNF-α inhibitory with IC50 values of 10.68 ± 0.02 μg/mL. In anti-free radical testing, the ethanol extract of D. loureiroi displayed high antioxidant activity by both ABTS and DPPH assays. Conclusion The ethanol extracts from carbonized T. grandis and Mahanintangtong showed good antimicrobial activity, especially against S. pyogenes, and good anti-inflammatory activity. These findings are relevant to the pathogenesis of pharyngitis and justify additional studies to see if Mahanintangtong could have clinical utility.

scholarly journals Aktivitas Antioksidan Fraksi Dietileter Buah Mangga Arumanis (Mangifera indica L.) dengan Metode DPPH

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Dewi Andini Kunti Mulangsri ◽  
Aqnes Budiarti ◽  
Endah Novia Saputri
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Diethyl Ether ◽  
Mangifera Indica ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Antioxidant Systems ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ether Fraction

ABSTRAK Sistem antioksidan merupakan bagian dari sistem kekebalan tubuh yang mampu menghambat reaktifitas radikal bebas. Sumber alami antioksidan salah satunya adalah buah yaitu buah mangga Arumanis. Proses fraksinasi dengan pelarut dietileter dilakukan untuk menyari senyawa-senyawa yang bersifat semipolar yang diharapkan memiliki potensi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan fraksi dietil eter ekstrak etanol buah mangga arumanis (Mangifera indica L.) dengan Metode DPPH. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, kemudian dipartisi cair-cair menggunakan pelarut dietil eter. Penentuan aktivitas antioksidan menggunakan metode DPPH dengan membuat seri konsentrasi fraksi dietil eter ekstrak etanol buah mangga Arumanis yaitu 12,5; 25; 50 dan 100ppm. Vitamin C digunakan sebagai larutan pembanding dengan seri konsentrasi 0,625; 1,25; 2,5 dan 5 ppm. Hasil penelitian menunjukkan bahwa fraksi dietil eter ekstrak etanol buah mangga arumanis memiliki aktivitas antioksidan dengan nilai IC50 sebesar 75,22 ppm dan 1,18 ppm untuk Vitamin C. Fraksi dietileter ekstrak etanol buah mangga Arumanis memiliki potensi lemah sebagai antioksidan jika dibandingkan dengan Vitamin C. Kata Kunci: antioksidan, fraksi dietileter, buah mangga arumanis, Mangifera indica, DPPH ABSTRACT Antioxidant systems are part of the immune system which is able to inhibit the reactivity of free radical. Source of natural antioxidants is a fruit that is mango Arumanis. Process of fractionation with solvent diethylether to extraction the compounds semipolar and expected to have potential as antioxidants. This study aims to determine the antioxidant activity of diethyl ether fraction of ethanol extract Arumanis mango (Mangifera indica L.) with DPPH method. Extraction used ethanol 70% by maceration method, then partitioned using a liquid-liquid partition with diethylether as a solvent. Determination of antioxidant activity using DPPH method with a series of concentrations of diethyl ether fraction of ethanol extract Arumanis mango ie 12.5; 25; 50 and 100ppm. Vitamin C used as a reference solution with a concentration series of 0,625; 1.25; 2.5 and 5 ppm. The results showed that the diethyl ether fraction of ethanol extract Arumanis mango have antioxidant activity with IC50 value of 7.22 ppm and 1.18 ppm for Vitamin C. Diethylether fraction of ethanol extract Arumanis mango has weak potential as a antioxidant, if compared with Vitamin C. Keywords: Antioxidants, diethylether fractions, arumanis mango fruit, Mangifera indica, DPPH.

Phytochemical Profile, Antimicrobial and Antioxidant Activities of Cistus clusii Essential oil From Algeria

2020 ◽  
Vol 16 (2) ◽  
pp. 109-114
Author(s):  
Souad Khebri ◽  
Naima Benkiki ◽  
Mouloud Yahia ◽  
Hamada Haba
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Β Carotene

Objective: In this work, we investigated the chemical composition and the antioxidant and antimicrobial activities of the Algerian Cistus clusii Dunal essential oil. Methods: Cistus clusii essential oil has been extracted using hydrodistillation method and characterized by GC/MS. The antioxidant activity was evaluated by two different methods DPPH free radical scavenging, and β-carotene bleaching test. The antimicrobial activity was carried out using disc diffusion method against reference strains; Pseudomonas aeruginosa (ATCC 27865), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), clinical bacterial (Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus) and fungal (Candida albicans) isolates. Results: The chemical profile of the oil showed 31 compounds. This profile was dominated by the presence of monoterpene hydrocarbons (78.4%), oxygenated derivatives (18.8%) and sesquiterpene hydrocarbons as minor constituents of this essential oil (1.9%). The major constituents were Camphene (20.6%), γ-Terpinene (16.5%), Sabinene (12.8%), and Terpineol-4 (13.5%). Other compounds were found in moderately good amounts like α-Terpinene (8.9%), α-Thujene (5.9%), α-Pinene (4.2%), and α- Terpinolene (3.1%). The results of the antimicrobial activity showed that the highest activity was observed against C. albicans and it was higher than positive control Fluconazole. The essential oil exhibited a strong antioxidant activity, especially in the β-carotene system. Conclusion: This study indicates that Cistus clusii essential oil showed potent antioxidant and moderate antimicrobial activities which could be used to explain the use of this species in traditional medicine.

scholarly journals Preliminary Chemical, Total Polyphenol, Total Flavonoid Contents and Antioxidant Activity of Eucalyptus camaldulensis Dehnh.

2020 ◽  
Vol 32 (5) ◽  
pp. 1230-1234 ◽  
Author(s):  
Nguyen Van Thuy ◽  
Nguyen Minh Tien ◽  
Nguyen Ngoc Quy ◽  
Mai Huyng Cang ◽  
Pham Minh Quan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Aqueous Extract ◽  
Eucalyptus Camaldulensis ◽  
Ethanolic Extract ◽  
Total Flavonoid ◽  
Ether Extract ◽  
Total Polyphenol ◽  
Diethyl Ether Extract ◽  
Pharmacologically Active

This study aimed to identify phytochemicals and evaluate antioxidant activities of different extracts from Eucalyptus camaldulensis, an aromatic plant in the Myrtaceace family. Examined extracts in different solvents such as diethyl ether extract (DEE), the ethanolic extract (EE) and the aqueous extract (AE). Total polyphenol and total flavonoid contents were determined via aluminum chloride colorimetric method. Antioxidant activity was performed via ABTS and DPPH radical scavenging assays. The results showed that a wide variety of pharmacologically active compounds such as alkaloid, oil, flavonoid, triterpenoid, saponin, tannin and polyphenol were present in the leaves of Eucalyptus camaldulensis. The result of quantitative determination showed that total polyphenol content of the diethyl ether extract, ethanolic extract and aqueous extract achieved 73.47 ± 1.64, 306.48 ± 3.87 and 76.47 ± 1.64 mgGAE/g, respectively. Meanwhile, total flavonoid content was 41.74 ± 2.21, 45.98 ± 1.79 and 18.05 ± 0.81 mgQE/g, respectively. The ethanolic extract exhibited the highest DPPH (IC50 = 10.52 ± 0.14 μg/mL) and ABTS (IC50 = 9.86 ± 0.17 μg/mL). These results indicate that Eucalyptus camaldulensis leaves can be used in dietary applications with the potential to reduce oxidative stress.

scholarly journals Phytochemical screening and evaluation of Berginia ligulata root extract for antimicrobial activity

2013 ◽  
Vol 14 (3) ◽  
pp. 101-104
Author(s):  
Rishi Kumar Shukla
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Diethyl Ether ◽  
Pathogenic Bacteria ◽  
Petroleum Ether Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Root Extract ◽  
Phytochemical Screening ◽  
Bacterial Strains

Berginia ligulata popularly known as paashaanbhed belongs to family Saxifragaceae. The present study was designed to screen out various phytoconstituents present in the roots of Berginia ligulata and further its antimicrobial activity against human pathogenic bacteria. For this roots of the plant were shade dried, powdered and extracted using different solvents in increasing order of polarity i.e. petroleum ether, diethyl ether, acetone, ethanol and methanol respectively. Preliminary phytochemical screening of the extracts revealed the presence of steroids, terpenoids, alkaloids, tannins, flavanoids, glycosides and carbohydrates. The presence of these bioactive constituents is associated with the antimicrobial activity of the plant. Antimicrobial activity of extracts was performed by disc diffusion method at a concentration of 100% and 50% (w/v) against five bacterial strains. The maximum antimicrobial activity was found in ethanol extract followed by acetone, methanol, diethyl ether and least in petroleum ether extract respectively. Ethanol extract revealed broad spectrum of inhibition against S. sureus and P. aeruginosa.

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