scholarly journals Foliar anti-diabetic and antioxidant potential of a promising accession of Amaranthus hypochondriacus L.: GC-MS based evidences

2018 ◽  
Vol 7 (2) ◽  
pp. 121-126
Author(s):  
Manashi Aditya ◽  
◽  
Soumen Bhattacharjee ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Hydroxyl Radical ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Amaranthus Hypochondriacus ◽  
Chelating Activity ◽  
Metal Chelating

The present study makes an effort to investigate the foliar neutraceutical potential of a promising green accession of a seed amaranth (Amaranthus hypochondriacus, accession no. IC94661) based on anti-lipid peroxidation property, reducing power, metal chelating activity, hydroxyl radical scavenging property, antidiabetic factor and GC-MS based identification of hydroxyl containing phytochemicals. Methanol and aqueous fractions possessed exhibited better anti-lipid peroxidation, reducing, hydroxyl radical scavenging, and metal chelating properties in the experimental accession. Estimation of anti-diabetic factors from the young leaf extract also revealed significantly high α-glucosidase and α- amylase inhibition properties. When GC-MS study was carried out, it exhibited presence of several hydroxyls containing phytochemicals in the accession, some of which are having antioxidant properties. Taken as a whole, the data not only provide evidence of rich sources of marker antioxidant properties but also the availability of several phytochemicals with hydroxyls, in support of its rich pharmaceutical potential

scholarly journals Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

2009 ◽  
Vol 6 (2) ◽  
pp. 227-231 ◽  
Author(s):  
S. A. Adesegun ◽  
A. Fajana ◽  
C. I. Orabueze ◽  
H. A. B. Coker
Keyword(s):  
Ascorbic Acid ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

scholarly journals IN VITRO - ANTIOXIDANT AND PRELIMINARY PHYTOCHEMICAL ANALYSIS OF AEGLE MARMELOS (L.) CORREA. LEAF EXTRACT

2017 ◽  
Vol 10 (6) ◽  
pp. 367
Author(s):  
Samidha M Pawaskar ◽  
Sasangan Kc
Keyword(s):  
Hydroxyl Radical ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Aegle Marmelos ◽  
Leaf Powder ◽  
Phenolic And Flavonoid

Objective: In this study, the leaf powder of Aegle marmelos (L.) Correa. was subjected to preliminary phytochemical and in vitro antioxidant analysis. Methods: The freshly prepared plant leaf extract was subjected to preliminary phytochemical screening, which revealed the presence of alkaloids, tannins, saponins, flavonoids, glycosides, phenolic compounds, terpenoids, and steroids. Reducing power, superoxide (SO) anion radical, nitric oxide (NO) radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the methanolic leaf extract of this plant. The amounts of total phenolic and flavonoid compounds were also determined. Results: This study has revealed that the A. marmelos (L.) Correa. leaf extract showed considerably high amounts of most of the phytochemicals, total antioxidant capacity, total phenolic, and flavonoid content. The study also indicated that the A. marmelos (L.) Correa. showed comparatively good scavenging activity, i.e., inhibition of hydroxyl radical, NO and SO anion scavenging and reducing power activities when compared with the respective standards. Conclusion: The leaf powder of A. marmelos (L.) Correa. can be used as easily accessible source of natural antioxidant and as a possible food supplement or in pharmaceutical industry.

scholarly journals GC-MS Analysis and Antioxidant Potential of Essential Oil from Endemic Sideritis rubriflora Hub.-Mor.

Proceedings ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 24
Author(s):  
Ceylan ◽  
Zengin ◽  
Aktümsek
Keyword(s):  
Essential Oil ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Essential Oil Composition ◽  
Antioxidant Potential ◽  
Oil Composition ◽  
Metal Chelating ◽  
Natural Agents ◽  
Mediterranean Regions ◽  
Shed Light

The genus Sideritis, belonging to the Labiateae family, is represented by more than 150 species distributed mainly in the Mediterranean regions. Member of the genus have been used as beverages, flavorings, and medication. We aimed to shed light on the antioxidant potential of the essential oil of Sideritis rubriflora Hub.-Mor. In this study, antioxidant capacity (DPPH and ABTS radical scavenging, CUPRAC and FRAP, phosphomolibdenum assay, and metal chelating activity) of essential oil of S. rubriflora was investigated with colorimetric methods. Also, essential oil composition of the plant was determined by gas chromatography/mass spectrophotometry (GC-MS). 48 components, representing 95.4% of essential oil of S. rubriflora were identified. β-pinene (10.7%) and Germacrene D (10.7%) were the main constituents of the essential oil. Generally, essential oil of S. rubriflora has shown moderate free radical, reducing power, metal chelating. The reported results supported that the possible use of essential oil of S. rubriflora is a source of natural agents for phytopharmaceutical applications.

scholarly journals Phytochemical evaluation and in vitro antioxidant studies of selected species of Gomphostemma Wall. ex Benth. from Western Ghats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 32-37
Author(s):  
M. K Sajitha ◽  
J. E. Thoppil
Keyword(s):  
Hydroxyl Radical ◽  
Western Ghats ◽  
Quantitative Estimation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Dpph Assay ◽  
Radical Scavenging Assay

Plants are a rich source of bioactive compounds and have played a vital role in traditional medicine. Plant derived compounds are proved to have less side effects as compared to synthetic compounds. However, a truncated history on the contribution of plants to medicine together with lack of validation of plant derived components have led to massive research in phytochemistry and allied fields. Present study was intended to identify as well as to assess and compare different phytochemicals and antioxidant potential of selected taxa of Gomphostemma endemic to Western Ghats. Methanolic extracts of aerial parts of Gomphostemma heyneanum var. heyneanum, Gomphostemma heyneanum var. rottleri and Gomphostemma eriocarpum were subjected to preliminary phytochemical screening. Quantitative estimation of phenols and flavonoids was done with Folin-Ciocalteu method and modified Aluminium chloride method respectively. Antioxidant potential was examined by DPPH assay, Hydroxyl radical scavenging assay and Reducing power activity. Preliminary screening indicated the presence of many secondary metabolites like phenols, flavonoids, alkaloids, terpenoids and steroids. Results of quantitative estimation revealed that G. heyneanum var. heyneanum has the highest concentration of phenols and flavonoids. Antioxidant potential determined in terms of inhibition percentage also displayed that Gomphostemma species have significant radical scavenging activity. The antioxidant potential exhibited by the plant species may be attributed to their phenol and flavonoid content. Keywords: Phytochemical constituents, Gomphostemma, Quantitative estimation, Total phenolics, Flavonoids, Antioxidant activity, DPPH assay, Hydroxyl radical scavenging assay, Reducing power activity.

scholarly journals Antioxidant Potential, Antinutrients, Mineral Composition and FTIR Spectra of Legumes Fermented with Rhizopus oligosporus

2021 ◽  
Vol 59 (4) ◽  
Author(s):  
Barinderjeet Singh Toor ◽  
Amarjeet Kaur ◽  
Param Pal Sahota ◽  
Jaspreet Kaur
Keyword(s):  
Mineral Composition ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Pigeon Pea ◽  
Ftir Spectra ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Rhizopus Oligosporus ◽  
Antioxidant Power

Research background. Legumes are superior sources of macro- and micronutrients which can be further enhanced by fermentation. This can assist in addressing the food security concerns. The present study aims to determine the effect of fermentation by Rhizopus oligosporus on nutritional and antinutritional composition of some commonly consumed legumes. Experimental approach. Chickpea (kabuli and desi), pigeon pea, and soybean were fermented with Rhizopus oligosporus (at 34 °C for 52 h), dried at 45 ºC for 16-18 h and milled. Unfermented and fermented flours were evaluated for antioxidant potential, phenolic composition, antinutrients, mineral composition and FTIR spectra. Results and conclusions. Fermentation significantly (p<0.05) enhanced the total phenolic and flavonoid contents, and antioxidant properties (radical scavenging activity, reducing power, ferric reducing antioxidant power and metal chelation) of chickpea kabuli and desi, and soybean. Although fermented pigeon pea exhibited excellent antioxidant properties, the effect of fermentation on such properties was either minimal or insignificant. Additionally, specific phenolics were quantified using HPLC which showed higher concentration of certain compounds such as chlorogenic acid, p-hydroxybenzoic acid, gallic acid and vanillic acid in fermented legumes. Phytic acid in all the fermented legumes reduced (p<0.05), however trypsin inhibition increased (p<0.05). In chickpea kabuli and desi, and pigeon pea, saponins increased (p<0.05) while they decreased in soybean. Tannins enhanced (p<0.05) in chickpea desi, pigeon pea and soybean and reduced (p<0.05) in chickpea kabuli. Furthermore, fermentation enhanced the content as well as estimated bioavailability of minerals. FTIR spectrum of unfermented and fermented legumes showed the presence of several functional groups and modifications in the molecular structure after fermentation. Novelty and scientific contribution. To our knowledge, this is the first study wherein legume (chickpea kabuli and desi, pigeon pea and soybean) fermentation by Rhizopus oligosporus has been assessed for nutritional and antinutritional profile, and FTIR spectra, which concluded that the treatment resulted in an optimal balance of nutrients and antinutrients. The process was established as a potential tool and thus can be proposed in the development of legume based novel functional foods which might help in tackling the concerns of nutritional security.

scholarly journals Physicochemical and antioxidant properties of freeze-dried dromedary skim colostrum and milk powder

Mljekarstvo ◽  
2020 ◽  
Vol 71 (1) ◽  
pp. 69-78
Author(s):  
Olfa Oussaief ◽  
Keyword(s):  
Bulk Density ◽  
Freeze Drying ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Milk Powder ◽  
Skim Milk ◽  
Reducing Power ◽  
Skim Milk Powder ◽  
Chelating Activity

This study aimed to determine the physicochemical properties and antioxidant activities of dromedary skim colostrum and milk powder produced by freeze-drying. Results of the study showed that skim colostrum powder possessed higher protein content compared to milk powder whereas this latter had greater lactose and ash content. The analysis of mineral content revealed that calcium and magnesium levels were higher in skim colostrum powder while the iron level did not differ significantly between skim colostrum and milk powder. The measurements of colour characteristics indicated that dromedary skim colostrum powder was redder, but less yellow and white than dromedary skim milk powder. Further, dromedary skim milk powder had higher bulk density and tapped bulk density. Protein solubility of skim colostrum powder exceeded that of skim milk powder over a wide range of pH (3-8). The antioxidant activities were evaluated using various in vitro tests, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activities, ferric reducing power assay and ferrous chelating activity. Both dromedary skim colostrum and milk powder exhibited antioxidant activities in a dose dependent manner. DPPH and ABTS radical scavenging activities were almost similar for skim colostrum and milk powder whereas ferric reducing power and ferrous chelating activity were more pronounced in dromedary skim colostrum powder whatever the concentration tested. Hence, freeze-drying process could be used as an effective tool for producing powder from dromedary skim colostrum and milk with nutritional and antioxidant properties.

scholarly journals FREE-RADICAL SCAVENGING ACTIVITY LEAF EXTRACT OF LITSEA LAEVIGATA GAMBLE

2019 ◽  
pp. 96-103
Author(s):  
S. SUJATHA ◽  
T. SEKAR
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Metal Chelating

Objective: In the present study, antioxidant activity in the leaf of the pet-ether, chloroform, acetone and methanolic extracts from Litsea laevigata Gamble. Leaf was investigated by employing established in vitro studies. L. laevigata belongs to the Lauraceae family. Methods: The capability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals were determined by in vitro antioxidant assays using 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH.) scavenging, reducing power assay, superoxide radical (O2*-) scavenging activity, phosphomolybdenum assay, FRAP, ABT and metal chelating activity were performed to know the antioxidant potency of the plant extract of leaves of L. laevigata. Results: Results are evaluated higher in leaf extract of L. laevigata recorded total phenol, total flavonoid, and tannin. The present state of work was designed to evaluate the phytochemical, antioxidant in the plant leaf extracts of L. laevigata. The plant L. laevigata methanolic extract of leaf showed greater IC50 antioxidant activity of DPPH assay (5.264 µg/ml) and compare to other extract, higher phosphomolybdenum reduction (164.36 mg/g), better Reducing power activity leaf in methanol (0.711%), higher ferric reducing power (4060.66MmolFe(II)E/mg), and higher in superoxide radical scavenging activity in (78.12 mg/ml). However, the better metal chelating ability was shown by the water extracts of the leaf (5.145 EDTAE/100g) compared to other solvent extracts. Conclusion: The result indicates the total phenol and antioxidant activity potential of L. laevigata.

scholarly journals Inhibition of Fe2+ Induced Lipid Peroxidation in Rats Brain by Extract of Euphorbia heterophylla in vitro

2019 ◽  
pp. 1-8
Author(s):  
Leye Jonathan Babatola ◽  
Oluwakemisola B. Oshanimi ◽  
Olanrewaju M. Oluba ◽  
Lawrence Okoror ◽  
Adewale Agboola Odutuga
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenol ◽  
Dependent Manner ◽  
Significant Difference

This study is sought to determine the antioxidant activity and protective ability of aqueous and methanol extractible phytochemicals from Euphorbia heterophylla leaves on lipid peroxidation induced in rat brain by pro-oxidant, in vitro. The extracts of the leaves were prepared, and the ability of the extracts is to inhibit 25 µM FeSO4 induced lipid peroxidation in isolated rats’ brain, were determined. Thereafter, total phenol content, reducing power (FRAP), Fe (II) chelating, and DPPH* free radical scavenging ability of the extracts was determined and considered as an index of antioxidant activity. The results revealed that the extracts inhibit malondialdehyde (MDA) production in the basal and pro-oxidant induced lipid peroxidised rats in a dose-dependent manner, [methanol 80.11%, aqueous 70.3%] with the methanol extract (MEE) significantly (P< 0.05) than that of aqueous extract (AEE). The methanol extract (0.74 ± 0.6 mg/g) had higher total phenol contents than the aqueous (0.57 ± 1.2 mg/g); likewise the methanol extract had higher reducing power (0.08 ± 0.2, 0.03 ± 0.1 mg/g), but had no significant difference in Fe (II) chelating ability (EC50= 0.34, 0.36) with DPPH* scavenging ability (EC50=0.075, 0.075). This antioxidant properties and the protective effect of this leaf could be harnessed in the management and prevention of degenerative diseases in association with oxidative stress.

scholarly journals Cholinesterase, tyrosinase inhibitory and antioxidant potential of randomly selected Umbelliferous plant species and chromatographic profile of Heracleum platytaenium Boiss. and Angelica sylvestris L. var. sylvestris

2016 ◽  
Vol 81 (4) ◽  
pp. 357-368 ◽  
Author(s):  
Ilkay Orhan ◽  
Fatma Tosun ◽  
Krystyna Skalicka-Woźniak
Keyword(s):  
High Throughput Screening ◽  
High Performance ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Cholinesterase Inhibition ◽  
Metal Chelating ◽  
Low Profile ◽  
Nitric Oxide Radical ◽  
Chromatographic Profile

Neurobiological activity of the methanol extracts of thirteen Umbelliferae (Apiaceae) plants was tested against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR) using high-throughput screening technique. Although the extracts displayed none to low profile of inhibition against enzymes, the highest cholinesterase inhibition was observed with Heracleum platytaenium (32.52 ? 3.27 % for AChE and 46.16 ? 1.42 % for BChE) at 100 ?g mL-1. Since neurodegeneration is linked to oxidative damage, antioxidant potential of the extracts was searched through radical scavenging, metal-chelating capacity, and reducing power experiments and exerted modest levels of activity varying according to the method. The extracts had a better ability to scavenge nitric oxide radical (19.47 ? 2.09 % to 54.91 ? 1.98 %). Since these species are known to be rich in coumarins, our quantitative high-performance liquid chroatography (HPLC) analysis indicated presence of xanthotoxin, angelicin, isopimpinellin, bergapten, and pimpinellin in Heracleum platytaenium and angelicin and imperatorin in Angelica sylvestris var. sylvestris.

scholarly journals Antioxidant Potential of Chestnut (Castanea sativa L.) and Almond (Prunus dulcis L.) By-products

2010 ◽  
Vol 16 (3) ◽  
pp. 209-216 ◽  
Author(s):  
J.C.M. Barreira ◽  
I.C.F.R. Ferreira ◽  
M.B.P.P. Oliveira ◽  
J.A. Pereira
Keyword(s):  
Lipid Peroxidation ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Castanea Sativa ◽  
Thiobarbituric Acid ◽  
Waste Products ◽  
By Products

The antioxidant properties of almond green husks (Cvs. Duro Italiano, Ferraduel, Ferranhês, Ferrastar and Orelha de Mula), chestnut skins and chestnut leaves (Cvs. Aveleira, Boa Ventura, Judia and Longal) were evaluated through several chemical and biochemical assays in order to provide a novel strategy to stimulate the application of waste products as new suppliers of useful bioactive compounds, namely antioxidants. All the assayed by-products revealed good antioxidant properties, with very low EC50 values (lower than 380 μg/mL), particularly for lipid peroxidation inhibition (lower than 140 μg/mL). The total phenols and flavonoids contents were also determined. The correlation between these bioactive compounds and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of lipid peroxidation in pig brain tissue through formation of thiobarbituric acid reactive substances, was also obtained. Although, all the assayed by-products proved to have a high potential of application in new antioxidants formulations, chestnut skins and leaves demonstrated better results.

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