scholarly journals Trapping Activity of Nematode-Trapping Fungus Arthrobotrys dactyloides on the Presence of Sawdust, Microorganisms, and Nematodes

2012 ◽  
Vol 13 (3) ◽  
pp. 178
Author(s):  
Sudirman Sudirman ◽  
Karwati Zawani

Once biological control agents of nematodes are introduced into soil, they must function in a very complex and dynamicenvironment. In soil, both nematodes and their antagonists are influenced by other biotic and abiotic factors. However,our knowledge of the effects of these factors in soil is limited. Hence, instead of focusing on the role of individualorganisms in controlling nematode pests, this research aimed to determine the effects of sawdust, microorganisms,and nematodes on trapping activity of Arthrobotrys dactyloides. Experiments were conducted with “standardslide test” and “soil microcosm” using soil amended with various concentrations or without sawdust, with thepresence or the absence of microorganisms, and with the presence or the absence of nematodes (Caenorhabditiselegans and/or Meloidogyne javanica). The experiments were carried out with completely randomized design anddata were analyzed with analysis of variance followed by Tukey’s Honestly Significant Different test. Resultsshowed that sawdust did not have direct effect on ring formation and trapping activity of A. dactyloides. However,sawdust did have effect on microorganisms and C. elegans which then these two factors stimulated ring formationand trapping activity of A. dactyloides.

2020 ◽  
Vol 12 (3) ◽  
pp. 103
Author(s):  
TRI L. MARDININGSIH ◽  
ELNA KARMAWATI ◽  
TRI EKO WAHYONO

ABSTRAK<br />Sanurus indecora merupakan salah satu hama utama yang<br />menyerang tanaman jambu mete di Propinsi Nusa Tenggara Barat. Di<br />Lombok Timur, hama ini diserang oleh cendawan Synnematium sp.<br />Berdasarkan hal tersebut perlu penelitian untuk mengetahui apakah<br />cendawan tersebut dapat digunakan untuk mengendalikan S. indecora.<br />Penelitian yang bertujuan untuk mengetahui peranan Synnematium sp.<br />dalam pengendalian S. indecora dilakukan di Desa Pohgading, Kecamatan<br />Pringgabaya dan di Desa Wanasaba, Kecamatan Wanasaba, Kabupaten<br />Lombok Timur dari bulan Juni sampai September 2004. Penelitian terdiri<br />atas dua kegiatan yaitu di tingkat pot (bibit) dan lapang. Penelitian di<br />tingkat pot terdiri atas tiga kegiatan yaitu aplikasi cendawan terhadap telur,<br />nimfa, imago pada bibit jambu mete (10 telur/ serangga/ bibit). Rancangan<br />yang digunakan ialah acak lengkap dengan empat perlakuan dan diulang<br />enam kali. Pengamatan dilakukan terhadap jumlah telur atau serangga<br />yang mati karena terserang Synnematium sp. mulai satu sampai tujuh hari<br />setelah perlakuan. Penelitian lapang menggunakan rancangan acak<br />kelompok yang disusun secara faktorial dengan dua macam faktor yaitu<br />pola tanam dan konsentrasi Synnematium sp. Parameter yang diamati<br />adalah populasi S. indecora, tingkat serangan, jumlah bunga hermaprodit<br />sebelum perlakuan dan jumlah buah yang berkembang. Hasil penelitian<br />menunjukkan bahwa cendawan Synnematium sp. yang diaplikasikan pada<br />nimfa S. indecora dan bibit menyebabkan kematian serangga tertinggi<br />mencapai 98,33%. Kematian serangga pada ketiga macam konsentrasi<br />Synnematium sp. baik pada telur, nimfa dan imago tidak berbeda nyata.<br />Dalam penelitian ini konsentrasi terkecil yaitu 20 g/l atau setara dengan<br />konsentrasi spora 1,64 x 10 8  sudah efektif menyebabkan kematian S.<br />indecora. Penurunan populasi S. indecora oleh Synnematium sp. efektif<br />dengan konsentrasi 20 g/l sebesar 24,14% dibandingkan dengan kontrol.<br />Tingkat serangan berkorelasi positif dengan populasi serangga.<br />Kata kunci: Jambu mete, Anacardium occidentale L., hama, Sanurus<br />indecora, pengendalian hayati, Synnematium sp. Nusa<br />Tenggara Barat<br />ABSTRACT<br />The role of Synnematium sp. in controling Sanurus<br />indecora JACOBI (Homoptera : Flatidae)<br />Sanurus indecora is one of major pests attacking cashew plants in<br />West Nusa Tenggara Province. In East Lombok, this insect pest was<br />attacked by fungi of Synnematium sp. Based on that, the experiment was<br />conducted to find out whether the fungi could be used to control S.<br />indecora or not. The objective of the experiment was to examine the role<br />of Synnematium sp. in the controlling S. indecora. It was carried out in<br />Pohgading, Pringgabaya and Wanasaba, District of East Lombok from<br />June to September 2004. The experiment consisted of two activities<br />namely polybag stage and field activities. Polybag stage activities<br />consisted of three activities namely application of Synnematium sp. on<br />eggs, nymphs, adults on seedling (10 eggs/insect/seedling). The<br />experiment was arranged in a completely randomized design with four<br />treatments and six replications. Observation was conducted on the number<br />of dead eggs, nymphs and adults attacked by Synnematium sp. from one to<br />seven days after treatment. While field activities used a randomized block<br />design arranged in a factorial with two factors i. e. plant pattern and<br />concentration of Synnematium sp. Parameters observed were population of<br />S. indecora, the degree of attack, the number of hermaphrodite flowers<br />before application and the number of developed fruits. Research results<br />showed that Synnematium sp. sprayed to nymphs of S. indecora and<br />seedling caused the highest mortality of S. indecora that reached 98.33%.<br />Mortality of S. indecora on the three concentrations either on eggs,<br />nymphs and direct application to adults was not significantly different. In<br />this experiment, the smallest concentration i.e. 20 g/l or equivalent with<br />concentration of spore 1.64 x 10 8  was effective to cause the death of S.<br />indecora. The decrease of S. indecora population by Synnematium sp. was<br />effective with concentration of 20g/l as many as 24,14% compared with<br />control. The degree of attack was positively correlated with population of<br />S. indecora.<br />Key words: Cashew, Anacardium occidentale L., pest , Sanurus indecora,<br />biological control, Synnematium sp. Nusa Tenggara Barat


2021 ◽  
Vol 226 ◽  
pp. 00022
Author(s):  
Henik Sukorini ◽  
Feby Wirasdenty Aigahayunindy ◽  
Erfan Dani Septia ◽  
Netnapis Khewkhom

Fruit rot disease is very damaging to cacao pods, which is caused by Phytoptora palmivora. The attack rate of P. palmivora varies. In Java, losses due to this disease reduce yields by 90 %. P. palmivora is a soil–borne pathogen. It is currently included in the Kingdom Chromista. Control with fungicides is not successful at this time, the alternatives is biologis control with Trichoderma sp. This research used a Completely Randomized Design (CRD) which was arranged in factorial with two factors. The first factor was Trichoderma sp. the second factor was P. palmivora. All treatment combinations were repeated three times. Trichoderma sp. antagonist test to P. palmivora was analyzed using Analysis of Variance (ANOVA) and then further tested using a 5 % BNJ. Trichoderma sp. origin from Jember and Trenggalek districts, East Java, Indonesia were able to act as antagonists against P. palmivora with the highest inhibitory of 78 %. In comparison, the lowest inhibitory was 70 % of isolates from Jember district, East java, Indoe. Characteristics of Trichoderma sp. The origin of Trenggalek Regency and Jember Regency, East Java, Indonesia in inhibiting the growth of P. palmivora has the same species, namely Trichoderma harzianum.


2018 ◽  
Vol 48 (1) ◽  
pp. 36-42
Author(s):  
Francisco Tarcísio Lucena ◽  
Fernandes Antonio de Almeida ◽  
Gilberto Antonio Torres Junior ◽  
Maria Lúcia Tiburtino Leite ◽  
Wéverson Lima Fonseca ◽  
...  

ABSTRACT Lima bean is one of the most popular leguminous plants in northeastern Brazil, with a strong socioeconomic influence on the regional communities. However, the low yield related to biotic and abiotic factors limits the increase in production. Among the biological agents, root-knot nematodes compromise its vegetative and productive development. In order to select genotypes resistant to Meloidogyne javanica, an experiment was carried out in a greenhouse and in a laboratory, in a completely randomized design, with fifteen lima bean accessions and five replicates. The reaction of lima bean accessions was evaluated through the infectivity and reproduction of M. javanica, as well as the main agronomic traits of the crop. A variation was observed in the resistance level among the accessions, with Lavandeira preta, Orelha de vó and Fava cearense being rated as very resistant, while Lavandeira vermelha was slightly resistant and the remaining accessions were all rated as susceptible.


2017 ◽  
Vol 38 (5) ◽  
pp. 2995 ◽  
Author(s):  
Adriely Alves de Almeida ◽  
Vinicius Hicaro Frederico Abe ◽  
Ricardo Marcelo Gonçalves ◽  
Maria Isabel Balbi-Peña ◽  
Débora Cristina Santiago

The objective of this study was to evaluate the efficacy of the active ingredient abamectin, either in isolation or in combination with fungicides and insecticides formulated for the industrial seed treatment, on the population of Meloidogyne javanica, tested under greenhouse and in vitro conditions. In both tests, the combination of the following treatments was assessed: abamectin; thiamethoxam; fludioxonil + metalaxyl-M + thiabendazole. Water was used as control for the in vitro assay, whereas under greenhouse conditions, controls were inoculated and not inoculated with M. javanica. The tests were conducted in a completely randomized design with six (in vitro) and ten (greenhouse) replicates. For in vitro studies the effect on hatching, motility, and mortality of juveniles of M. javanica was evaluated. Under greenhouse conditions, the soybean ‘BRS 133’ seeds were treated, and at 15, 30, and 60 days after inoculation (DAI) with M. javanica, plant measurements were recorded. The penetration of second stage juveniles (J2) was also evaluated at 15 DAI. At 30 DAI, galls, egg masses, nematodes/g of root, and final population were evaluated. At 60 DAI, the final population of nematodes in the roots was quantified. The treatments containing abamectin were the most effective in diminishing the hatching of juveniles. All treatments had an effect on nematode motility when compared to the control, and in the treatment containing only abamectin, total juvenile mortality was observed. In greenhouse conditions, at 15 DAI, the treated soybean plants had the highest root mass and shoot length, differing statistically from the inoculated control. All treatments reduced the number of nematodes per gram of root, differing from the control. At 30 DAI, treatment efficiency was observed in reducing the final population of M. javanica, particularly the treatments using abamectin, and abamectin + thiamethoxam + fludioxonil + metalaxyl-M + thiabendazole. However, at 60 DAI, the effect of the treatments on the population of M. javanica did not persist.


2011 ◽  
Vol 4 (3) ◽  
pp. 332-340 ◽  
Author(s):  
Kimberly K. Crider

AbstractQuantification of interference with biological control agents can provide support for anecdotal claims of success or failure of agent establishment and efficacy. This study was initiated because of observed predation of cinnabar moth larvae by carpenter ants when releasing larvae for the control of tansy ragwort, an invasive plant in Montana. Biotic and abiotic factors were compared among three sites with historically variable moth population establishment. Two experiments were developed to (1) observe and document insect activity, predation, or disappearance on tansy ragwort stems either protected or accessible to ants; and (2) quantify the effects of ant exclusion on herbivory of tansy ragwort. Site comparisons indicated that ant colony density was highest at the driest of three sites, and, interestingly, no ant colonies were detected at the site with higher observed numbers of moth larvae and adults and lower densities of tansy ragwort. Available substrate (logs and stumps) for ant colonization did not differ between the three sites. In the ant exclusion experiments, a larger number of larvae were missing on plants accessible to ants (63%) compared with plants where ants were excluded (39%) after 36 h. Direct observation of predation of larvae by carpenter ants accounted for 9% of missing larvae on stems accessible to ants. Larvae were able to consume 81% of original flowers or buds on ant-excluded stems, compared with 18% consumption on ant-accessible stems, suggesting that ant predation could limit the efficacy of cinnabar moth larvae. These results provide one of many possible explanations for the anecdotal observations of large, persistent populations of cinnabar moths in moist areas. This work emphasizes the importance of post-release observation and monitoring to detect and, ideally, quantify factors to support anecdotal perceptions regarding the fate and subsequent efficacy of insect biological-control agents.


2020 ◽  
Vol 3 (01) ◽  
pp. 28-39
Author(s):  
Sri Wilarso ◽  
Putri Maharani ◽  
Andi Sukendro ◽  
Cahyo Wibowo

Problems arising from silica sand mining are decreasing soil fertility, as well as the presence of high heavy metals such as Fe and Al, which inhibits the growth of revegetation plants. The purpose of this study was to analyze the response of the growth of balsa seedlings (Ochroma bicolor Rowlee.) on silica sand post-mining media treated with MycoSilvi, compost, and lime and determine the most optimal combination of MycoSilvi, compost, and lime treatment. This study uses a completely randomized design (CRD) with a split-plot design with 3 treatment factors, namely the addition of MycoSilvi, compost, and lime. Each treatment consisted of five replications. The results showed that the planting media that were not treated (control) was not able to support the growth of balsa seedlings. The interaction of MycoSilvi, compost, and lime significantly affected the height, diameter, biomass, and percentage of mycorrhizal colonization parameters, and significantly affected the plant chlorophyll content. The combination of MycoSilvi type 1 treatment, namely Glomus mosseae and lime (C0K1M1) type, resulted in the highest total growth rate, diameter, biomass, and colonization percentage compared to other treatments. The addition of lime and compost to the growth medium can reduce the degree of mycorrhizal dependency of balsa seedling.


2016 ◽  
Vol 8 (1) ◽  
pp. 24-28
Author(s):  
Maya Indra Rasyid

(Chemical and Organoleptic Characteristics of Mung Bean Flakes with The Addition of Fillers and Water) ABSTRACT. The objective of this research was to determine the chemical and organoleptic characteristics of mung bean flakes with the addition of fillers and water. The study was conducted using completely randomized design with two factors namely the addition of fillers and water. The results showed that the addition of fillers and water has significant (P≤0,01) effect on the water absorption ratio and protein levels of mung bean flakes. The best treatment was obtained by the mung bean flour without the addition of fillers with 150% of addition water with water absorption ratio of 204%, water content of 5,7%, protein of 22,01%, starch levels of 61,76%, organoleptic values of aroma of 2,61, color of 2,42, crispness of 2,72 and flavor of 2,83.


2012 ◽  
Vol 14 (1) ◽  
pp. 126
Author(s):  
Yekki Yasmin ◽  
Lenni Fitri ◽  
Betty Mauliya Bustam

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.


2021 ◽  
Vol 894 (1) ◽  
pp. 012030
Author(s):  
A T Maryani ◽  
N Mirna ◽  
F F Bahar

Abstract The use of chemical fertilizers in agriculture has caused soil degradation and is harmful to human health. Organic fertilizers made from animal waste have received worldwide attention because they are not detrimental to health or the environment. This paper describes the effect of using organic fertilizers from chicken manure on the growth of papaya plants. A completely randomized design was used in this study using two factors and three repetitions; the variations of the fertilizers given were 0, 50, 100, 150, and 200 g. This study informed that organic chicken manure had a significant effect on the growth of oil palm and papaya plants. The best growth is oil palm and papaya plants given 200 g of organic fertilizer from chicken manure.


2020 ◽  
Author(s):  
Thiwakorn Ampapon ◽  
Bounnaxay Viennasay ◽  
Metha Wanapat

Abstract Background A need for research searching for alternative rumen enhancers warrants immediate attention. The in vitro fermentation experiment was conducted using factorial arrangement of two factors of roughage to concentrate and seven level of red amaranth leaf powder percentage of total substrate in a Completely randomized design (CRD). Two factors, namely Factor A was two ratio of roughage (R) to concentrate (C) at 60:40 and 40:60 and Factor B was level of red amaranth (Amaranthus cruentus, L) leaf powder (RALP) supplementation at 0, 2, 4, 6, 8, 10, and 12% of total dietary substrate. Results Red amaranth leaf powder (RALP) contained phytonutrients both condensed tannins and saponins in addition with high macro minerals (Ca, K, and Mg). This experiment revealed innovations of the RALP supplementation by enhancing rumen propionate (C3) production, reducing acetate (C2) to (C3) ratio, reducing protozoal population and mitigating methane (CH4) production. Furthermore, rumen dry matter degradation percentages were remarkably enhanced (P < 0.001) by increasing RALP supplementation. Conclusion Plants rich in phytonutrients and minerals such as red amaranth leaf powder (RALP) have a vital and promising role in modulating rumen fermentation, mitigating methane production, as well as increasing substrate degradability.


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