scholarly journals Antimicrobial Sensitivity Pattern of Pseudomonas Aeruginosa Isolated from a Tertiary Care Hospital

2020 ◽  
Vol 19 (2) ◽  
pp. 70-74
Author(s):  
Sabita Bhatta ◽  
Manoj Pradhan ◽  
Alina Singh ◽  
Raina Chaudhary ◽  
Yankpam Ibotomba Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Laboratory ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Care Hospital ◽  
Sensitivity Testing ◽  
Time Duration ◽  
Disk Diffusion Test ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern

Introduction: Pseudomonas aeruginosa (P. aeruginosa) is one of the major cause of nosocomial infection. Multi-drug-resistant P. aeruginosa (MDR PA) are increasingly encountered in clinical samples .Therefore, this study was undertaken to know the sensitivity pattern of P. aeruginosa and to detect MDR PA from clinical samples. Methods: This was a laboratory based retrospective-cross sectional study conducted at Department of Microbiology at a tertiary care referral centre in Kathmandu, Nepal from December 2018 to December 2019. Total of 200 isolates of P. aeruginosa were isolated from clinical samples. Non repetitive sample per patient was included in the study. Samples were processed according to standard methodology and antimicrobial sensitivity testing (AMST) was carried out by Modified Kirby Bauer disk diffusion test as per Clinical Laboratory Standard Institute guideline. MDR was defined as strains resistant to one or more agent of ≥ 3 groups of antimicrobial categories. Results: Highest number of P. aeruginosa were isolated from sputum (93), followed by wound swab (35), and pus (29). AMST revealed the most sensitive drug to be Amikacin (91.51%) followed by Meropenem (78.5%) and Piperacillin/Tazobactam (77.50%). Out of 200 isolates, 69 (34.5%) were MDRPA. Conclusions: The information regarding sensitivity pattern of P. aeruginosa will help clinicians to choose most effective antimicrobials for the treatment of patients thus saving the time duration and total cost of patient management and also it will play a key role in setting antimicrobial stewardship policy.

scholarly journals Pseudomonas aeruginosa Infection and Antibiotic Sensitivity Pattern Isolated from a Tertiary Care Hospital in Dhaka City

2021 ◽  
pp. 23-29
Author(s):  
Mousumi Karmaker ◽  
Md. Abul Khair ◽  
Una Jessica Sarker ◽  
Rabeya Nahar Ferdous ◽  
Sa’dia Tasnim ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Health Sciences ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Care Hospital ◽  
Sensitivity Pattern

Pseudomonas aeruginosa is one of the most widespread gram-negative microorganisms identified in the clinical samples and most common causes of hospital acquired infection. P. aeruginosa is affecting both indoor and outdoor patients throughout the world. Due to frequent mutation in          P. aeruginosa highly resistant strain developed rapidly. The aim of the study to determine the prevalence of P. aeruginosa species in different samples isolated from a Tertiary care Hospital as well as determination their diverse antibiotic resistance pattern. This cross-sectional study was carried out to determine in-vitro resistance pattern of P. aeruginosa isolates to common antimicrobial agents by disc diffusion method. Various clinical samples were collected from Bangladesh Health Sciences Hospital (BIHS) General Hospital, Dhaka. This research was carried out in the Department of Microbiology of Bangladesh University of Health Sciences (BUHS). Isolation, identification and antibiogram were performed for P. aeruginosa following standard microbiological laboratory procedure. A total of 218 P. aeruginosa were isolated from 3062 different clinical specimens which are statistically significant (p<0.0001). Among the highest number of P. aeruginosa were isolated from outdoor patients 140 compare to Indoor patients which are significantly higher (p <0.013). In this study Male (68.3%) are more vulnerable to P. aeruginosa infection compare to females (31.7%) which is also statistically significant. Young people (less than 35 years) were more susceptible to P. aeruginosa infection which is also statistically significant (p< 0.01). The highest number of P. aeruginosa was isolated from wound (43.12%), followed by pus (40.33%), sputum (8.71%) urine (7.80%). The maximum number of P. aeruginosa in various samples was resistant to aztreonam and co-tromoxazole followed by cephalosporins, aminoglycosides, carbapenems. The most sensitive antibiotic was colistin of followed by gentamycin and tetracycline. To control the spread of resistant bacteria, it is disparagingly vital to have stringent antibiotic guidelines. The antibiotic susceptibility pattern of P. aeruginosa requires to be continuously monitored in specialized clinical units and the results readily made available to the clinicians to minimize the resistance.

scholarly journals ANTIMICROBIAL SENSITIVITY PATTERN OF ESCHERICHIA COLI ISOLATES FROM VARIOUS CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL

2020 ◽  
Vol 8 (12) ◽  
pp. 774-779
Author(s):  
Anupama S. Wyawahare ◽  
◽  
Jyotsna K Mishra ◽  
Manjushree V. Mulay ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Agents ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Care Hospital ◽  
E Coli ◽  
Antimicrobial Susceptibility Pattern ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern

E Coli is the most commonly encountered human pathogen which has been associated with various clinical manifestations.Analyzing resistance pattern of Escherichia coli to commonly prescribed antibiotics helps to decide empirical therapy.The study was conducted from January 2015 to December 2018.Total of 2135 E coli isolates were recovered from various clinical specimens .In our study antimicrobial susceptibility pattern of 1925 E coli isolates was studied Maximum no of E coli were isolated from urine 867 [40.60%] followed by skin & soft tissue specimens 691 [32.36%],stool specimens 179 [8.38%] , Respiratory Tract specimens 129[6.04%] , and blood 59 [2.76] .Out of 867 E coli isolates from urine specimens maximum susceptibility was found against Fosfomycin 806 [92.96 %] followed by Amikacin 753 [86.85 %], Ertapenem 702[80.96%], Gentamicin 563[64.93 %] and Nitrofurantoin 518[59.74% ]Most isolates of E coli from UTI, SSTI, RTI, BSI are susceptible to Carbapenems , Amikacin and CefoperazoneSulbactam..Increasing resistance to oral antimicrobial agents is a serious issue.Improved facilities for microbiology laboratories and judicious use of antimicrobial agents is need of the hour.

scholarly journals A Detailed Study of Antimicrobial Sensitivity Pattern of Penton Valentine Leucocidin Gene Positive and Negative Staphylococcus aureus from Pus Samples

2020 ◽  
Vol 34 (3) ◽  
pp. 24-28
Author(s):  
Mateen Izhar
Keyword(s):  
Staphylococcus Aureus ◽  
Tertiary Care ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Care Hospital ◽  
Sensitivity Testing ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
One Year ◽  
Biology Laboratory

Introduction: Staphylococcus aureus harboring Panton Valentine Leucocidin gene are emerging and spreading worldwide. PVL gene was first identified by Noel Panton and Francis Valentine in 1932 who IC Pakistan only limited data is available regarding the effect of PVL gene on sensitivity pattern of Staphylococcus aureus. Therefore, this study was conducted to understand the antimicrobial sensitivity pattern of both PVL positive and negative Staphylococcus aureus isolates. Aims & Objectives: This study was conducted to understand the antimicrobial sensitivity pattern of both PVL positive and PVL negative Staphylococcus aureus isolated from pus samples received from various indoor and outdoor departments of a tertiary care hospital of Lahore. Place and duration of study: Microbiology and Molecular Biology Laboratory Shaikh Zayed Hospital Lahore. Duration of study is one year after the approval of research topic. Material & Methods: A total of 384 Staphylococcus aureus isolates from skin and soft tissue infections were identified and selected. Their antimicrobial sensitivity testing was done by Kirby disc diffusion method using Muller Hinton agar. Results: Frequencies of PVL gene in MRSA and MSSA were 51% and 44% respectively. Frequency of PVL gene was also found to be high in Ciprofloxacin sensitive, Gentamicin sensitive, Erythromycin resistant and fusidic acid resistant isolates. Conclusion: Almost half of Staphylococcus aureus isolates were found PVL positive. They were mostly multidrug resistant. The PVL positive Staphylococcus aureus isolates showed high resistance against antibiotics than PVL negative isolates

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