scholarly journals Phytochemical Investigation of Aerial Parts of Iraqi Cardaria draba

2020 ◽  
Vol 29 (2) ◽  
pp. 27-36
Author(s):  
Alaa M. Abd ◽  
Enas J. Kadhim
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Acid ◽  
High Performance ◽  
Chemical Constituents ◽  
Aqueous Methanol ◽  
Plant Materials ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Soxhlet Apparatus ◽  
Layer Chromatography

 The aim of this study was to study chemical constituents of aerial parts of Cardaria draba since no phytochemical investigation had been studied before in Iraq. Aerial parts of Cardaria draba were defatted by maceration in hexane for 72 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous Methanol 90% as a solvent extraction for 18 h, and fractionated with petroleum ether- chloroform (CHCl3)- ethylacetate- and n-butanol respectivly. The ethyl acetate, n-butanol, and n-butanol after hydrolysis fractions were investigated by high performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) for its phenolic acid and flavonoid contents. Flavonoids and phenolic acid derivative were isolated from the ethylacetate of leaf fraction and n-butanol after hydrolysis fraction of the aerial parts and identified by TLC, FTIR and HPLC. A various chromatographic and spectroscopic results shown the presence of luteolin, chlorogenic acid, caffeic acid, and resorcinol in aerial parts of C. draba.                                                                                                                              

scholarly journals High Performance Thin Layer Chromatography Fingerprinting Analysis of Piper betle L. Leaves

2021 ◽  
pp. 8-15
Author(s):  
Ramdas N. Kale ◽  
Ravindra Y. Patil
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Piper Betle ◽  
Soxhlet Apparatus ◽  
Rf Values ◽  
Pharmacologically Active ◽  
Layer Chromatography

Introduction: Many modern medicines used today based on plants and plant products. Piper betle is generally known as the betle vine, it is an important medicinal and recreational plant. High performance thin layer chromatography (HPTLC) is an advanced powerful analytical method with more separation power, high performance and superior reproducibility than classic thin layer chromatography (TLC). A chromatographic fingerprint of a plant extract is a chromatographic pattern of some common chemical constituents of pharmacologically active and/or chemical characteristics. Chromatographic fingerprints are useful in authentication and identification of plant. Objectives:  Objectives of present research was to establish HPTLC fingerprinting of methanolic extract of Piper betle L. leaves. Materials and Methods: Methanolic extract of Piper betle leaves was prepared using soxhlet apparatus. HPTLC studies were performed using a CAMAG HPTLC system equipped with automatic TLC sampler-4 (ATS 4), TLC scanner 4, and vision CATS 3.0 software. Results: The study revealed the presence of alkaloids with Rf value 0.65, flavonoids with Rf values 0.19, 0.29, 0.72, 0.95., and phenolic compound with Rf value 0.7. Conclusion: The HPTLC fingerprinting profile developed for the methanolic extract of Piper betle L. leaves will help in proper identification of the plant.Piper betle

scholarly journals CONTRIBUIÇÃO AO ESTUDO FITOQUÍMICO E ANÁLITICO DO Nasturtium officinale R. Br., BRASSICACEAE

2002 ◽  
Vol 3 (2) ◽  
Author(s):  
J. L. S. CARVALHO ◽  
O. G. MIGUEL
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Analytical Study ◽  
Analytical Techniques ◽  
Phytochemical Study ◽  
Nasturtium Officinale ◽  
Aerial Parts ◽  
Traditional Process ◽  
Layer Chromatography ◽  
Brassicaceae Family

O presente trabalho, realizou o estudo sobre Nasturtium officinale R. Br.; pertencente a família Brassicaceae, cujas partes aéreas do vegetal foram submetidas a estudo botânico para obtenção das características anatômicas do mesmo. Aliado realizou-se o estudo fitoquímico utilizando como técnica analítica a cromatografia em camada delgada, cromatografia em coluna, cromatografia gasosa, cromatografia líquida de alta eficiência e espectrometria de ultra violeta. Foram desenvolvidas tecnologias extrativas de particionamento por processo tradicional em Soxhlet, nos quais pode-se verificar eficiência e reprodutibilidade para fins farmacotécnicos, farmacológicos e preparativos. De acordo coma literatura consultada, verificou- -se a presença de compostos fenólicos simples e heterosídicos (fenilpropoanóides e flavonóides), saponinas (esteróidais e terpênicas policíclicas) e compostos contendo enxofre (glucosinolatos). De acordo com análise comparativa dos processos extrativos (tradicional e Soxhlet), pode-se referir que o processo Soxhlet apresentou melhor rendimento e rapidez, alémde apresentar-se termoestável para a concentração e isolamento de grupos estruturais, para fins analíticos, farmacotécnicos e farmacológicos. A CONTRIBUTION TO PHYTOCHEMICAL AND ANALYTICAL STUDY OF Nasturtium officinale R. Br., BRASSICACEAE Abstract The present is concerned to a study about Nasturtium officinale R. Br.; Brassicaceae family, which aerial parts were submitted to o botanical study to obtain its anatomic characteristics. It was also conducted the phytochemical study and using as analytical techniques in thin layer chromatography, column chromatography, gaseous chromatography, high performance liquid chromatography and ultraviolet spectrometry. It was developed extractive technologies of partition by traditional process in Soxhlet, through which it was possible to verify efficiency and reproducibility to pharmacothechinical and pharmacological methods, and to preparative objectives too. According to the literature, it was verified the presence of simple and heterosidic phenolic compounds (phenylpropanoids and flavonoids), saponinas (steroidal and policyclic terpenic) and compounds containing sulfur (glucosinolates). According to the comparative analysis of the extractive processes (traditional and Soxhlet), it was possible to say that the Soxhlet process presented a better performance and speed, besides being thermo-stable to the concentration and isolation of the structural groups , to pharcothecnical, pharmacological and analytical objectives.

scholarly journals PHYTOCHEMICAL INVESTIGATION OF LEAVES AND SEEDS OF CORCHORUS OLITORIUS L. CULTIVATED IN IRAQ

2018 ◽  
Vol 11 (11) ◽  
pp. 408 ◽  
Author(s):  
Hayder T Hasan ◽  
Enas J Kadhim
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Phenolic Acid ◽  
High Performance ◽  
Corchorus Olitorius ◽  
Octadecanoic Acid ◽  
Plant Materials ◽  
Petroleum Ether Fraction ◽  
Phytochemical Investigation ◽  
Ether Fraction

Objective: The aim of this study was to investigate chemical constituents of leaves and seeds of Corchorus olitorius since no phytochemical investigation had been done previously in Iraq.Methods: Leaves and seeds of C. olitorius were defatted by maceration in hexane for 24 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous methanol 85% as a solvent extraction for 24 h, and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The ethyl acetate, n-butanol, and n-butanol after hydrolysis fractions for each part (leaves and seeds) were analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and flavonoid contents. The petroleum ether fraction from the leaves was analyzed using Gas chromatography/mass spectrometer (GC/MS). Flavonoids and phenolic acid derivative were isolated from the ethyl acetate of leaf fraction and n-butanol after hydrolysis fraction of the seeds and identified by mass spectrometry, infrared, HPLC, and HPTLC.Results: The different chromatographic and spectroscopic results revealed the presence of luteolin, quercetin, astragalin, isoquercetin, catechins, and 3,5-dicaffeoylquinic acid (3,5-DCQA) in both leaves and seeds of C. olitorius and also 17-octadecynoic acid, 9-octadecanoic acid (oleic acid), hexadecenoic acid (palmitic acid), 9,12-octadecanoic acid (linoleic acid), octadecadien-1-ol (β-sitosterol), and α-tocopherol (Vitamin E) in the petroleum ether fraction of C. olitorius leaves.Conclusion: The results of the current study proved the presence of 3,5-DCQA, astragalin, and isoquercetin in the ethyl acetate fraction of C. olitorius leaves and catechin in the n-butanol after hydrolysis fraction of C. olitorius seeds. 

scholarly journals PHYTOCHEMICAL INVESTIGATION OF CHENOPODIUM MURALE (FAMILY: CHENOPODIACEAE) CULTIVATED IN IRAQ, ISOLATION AND IDENTIFICATION OF SCOPOLETIN AND GALLIC ACID

2017 ◽  
Vol 10 (11) ◽  
pp. 70
Author(s):  
Omer H Ahmed ◽  
Maha N Hamad ◽  
Noor S Jaafar
Keyword(s):  
Ethyl Acetate ◽  
Gallic Acid ◽  
Phenolic Acid ◽  
High Performance ◽  
Chemical Constituents ◽  
Ethyl Acetate Fraction ◽  
Absolute Methanol ◽  
Isolation And Identification ◽  
Chenopodium Murale ◽  
Phytochemical Investigation

  Objective: The aim of our study was to investigate chemical constituents of leaves of Chenopodium murale since no phytochemical investigation had been done previously in Iraq.Methods: Leaves of C. murale were macerated in absolute methanol for 2 days and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The ethyl acetate fraction was analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and coumarins contents. Coumarin derivative and phenolic acid were isolated from this fraction and identified by gas chromatography/mass spectrometry, infrared, ultraviolet, HPLC, and HPTLC.Results: The different chromatographic and spectroscopic results revealed the presence of gallic acid and coumarin.Conclusion: The results of the current study showed the presence of scopoletin and gallic acid in the ethyl acetate fraction of C. murale.

scholarly journals Chemical Constituents of Eremostachys macrophylla Montbr. & Auch. Aerial Parts

2020 ◽  
Vol 26 (2) ◽  
pp. 203-208
Author(s):  
Parina Asgharian ◽  
Abbas Delazar ◽  
Solmaz Asnaashari
Keyword(s):  
High Performance ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Reversed Phase ◽  
Spectroscopic Technique ◽  
Phytochemical Analysis ◽  
Aerial Parts ◽  
Soxhlet Apparatus ◽  
Snake Bites ◽  
Pure Compounds

Background: Eremostachys macrophylla Montbr. & Auch. is one of the wild growing species of herbs found in East Azerbaijan province of Iran. These species are used in folk medicine for the healing of wound, treatment of snake bites, rheumatism and joint pains. The primary aim of this study was to obtain natural pure compounds and this was done by subjecting the aerial parts of Eremostachys macrophylla Montbr. & Auch. to phytochemical analysis. Methods: The air-dried and crushed aerial parts were respectively extracted with n-hexane, dichloromethane (DCM) and methanol (MeOH) solvents using a soxhlet apparatus. The 10%, 20% and 40% of MeOH in water Sep-Pak fractions of the MeOH extract were subjected to a preparative reversed- phase high performance liquid chromatography (RP-HPLC). Also, the isolated pure compounds were identified by one-dimensional nuclear magnetic resonance (1D-NMR) spectroscopic technique. Results: The results obtained in this study showed the presence of seven pure components; (1) Lamalbide, (2) Sesamoside, (3) Phlomiol, (4) Verbascoside, (5) Luteolin-7-O- glucoside, (6) Apigenin-7-O- rutinoside and (7) Kaempferol-3-O- glucoside with iridoid, phenylethanoid and flavonoid structures. Conclusion: The results from the study demonstrated that the aerial parts of E. macrophylla could be a good source of iridoids, phenylethanoids and flavonoids.

scholarly journals Chemical constituents of aerial parts of Thymus gobicus and their cholinesterase inhibitory activities

2017 ◽  
Vol 17 (43) ◽  
pp. 14-17
Author(s):  
Odonbayar B ◽  
T Murata ◽  
N Matsumoto ◽  
Batkhuu J ◽  
K Sasaki
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Methyl Ester ◽  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
High Performance ◽  
Chemical Constituents ◽  
Aerial Parts ◽  
Acetone Water ◽  
Inhibitory Activities

From an acetone-water (3:2) extract of aerial parts of Thymus gobicus Czern. (31.1 g), compounds 1-8 were obtained using high-performance liquid chromatography. Based on spectroscopic data, the isolated compounds were identified as rosmarinic acid (1), monardic acid A (2), nepetoidin B (3), aromadendrin (4), apigenin (5), chrysoriol (6), apigenin 7-O-β-D-glucuronopyranoside (7), and apigenin 7-O-β-D-glucuronopyranoside methyl ester (8). Compound 2 was a (7R,8R)-diastereomer of lithospermic acid (2a). Although it was reported that the anti-allergic activity of lithospermic acid was higher than that of 2, the acetylcholine inhibitory activity of 2 was higher than that of lithospermic acid.

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