Clinical and Histopathological Relevance of Helicobacter pylori BabA2 Genotype

Introduction: H. pylori BabA is an outer membrane protein that mediates bacterial adherence to the gastric epithelium, triggers several pathways during the course of infection, and thus contributes to the disease development. Considering the variability in the presence of BabA coding gene (babA2) among H. pylori clinical strains, the aim of this study was to assess the relationship between the genotype status of H. pylori babA2 and the severity of clinical and histopathological outcomes. Methods: Gastric mucosal biopsy specimens were collected from 30 CLO test-positive patients, 16 with gastritis and 14 with peptic ulcer disease. Polymerase chain reaction was carried out to detect the presence of H. pylori-specific glmM gene and BabA coding gene (babA2). Histopathological examination was performed to evaluate the severity of H. pylori-associated gastric disease according to the Updated Sydney Classification System. Results: The glmM and babA2 genes were present in 100% and 86.7% of the tested H. pylori strains, respectively. Although higher degrees of inflammatory activity and H. pylori density were noted in babA2-positive biopsy specimens, there was no statistically significant association between babA2 genotype status and the severity of gastric disease. Conclusion: The babA2 genotype status of H. pylori may not be considered as a sole marker for determining the infection outcomes.

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Association between VEGF -2578 C> A Polymorphism with VEGF Serum Levels in Gastritis with Helicobacter Pylori

International Journal of Research and Review ◽

10.52403/ijrr.20210823 ◽

2021 ◽

Vol 8 (8) ◽

pp. 171-175

Author(s):

Tara Rizvira Monica ◽

Gontar Alamsyah Siregar ◽

Taufik Sungkar

Keyword(s):

Serum Levels ◽

Mucosal Damage ◽

Cross Sectional Study ◽

Endoscopic Examination ◽

Chain Reaction ◽

Cross Sectional ◽

Excess Production ◽

Polymerase Chain ◽

H Pylori ◽

The Relationship

Mucosal damage in people with gastritis causes the production of VEGF. VEGF is a neoangiogenesis function to repair damaged tissue. Excess production can cause cancer risk. VEGF genotype polymorphisms are thought to affect the production of serum VEGF levels. The aim of this study was to determine the relationship between VEGF - 2578 C> A polymorphism and serum VEGF levels in H. pylori gastritis. Method: cross-sectional study was conducted at H. Adam Malik General Hospital and Network Hospital with 100 samples. Endoscopic examination was performed to assess the gastric mucosa and a tissue biopsy was performed. The urea breath test (UBT) test and the Campylobacter like organism (CLO) test to determine H. pylori infection. VEGF - 2578 C> A was checked by Polymerase Chain Reaction (PCR). The data will be analyzed by univariate and bivariate. Result: One hundred people with gastritis, of which 59 people were infected with H. pylori. In this study, H. pylori infection did not have a significant relationship with VEGF levels. VEGF - 2578 C> A polymorphisms also had no relationship to serum VEGF levels. Conclusion: There is no correlation between VEGF - 2578 C> A polymorphism with VEGF serum levels (p> 0.05). Keywords: VEGF polymorphisms, VEGF - 2578 CA, H. pylori, Gastritis.

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Investigation of possible aetiological/triggering factors in porcine ear necrosis syndrome at a farrow-to-feeder pig system

Acta Veterinaria Hungarica ◽

10.1556/004.2021.00018 ◽

2021 ◽

Author(s):

Branislav Kureljušić ◽

Božidar Savić ◽

Vesna Milićević ◽

Nemanja Jezdimirović ◽

Oliver Radanović ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Crude Protein ◽

Protein Concentration ◽

Histopathological Examination ◽

Lymphocytic Infiltration ◽

Reaction Products ◽

Chain Reaction ◽

Gross Lesions ◽

Polymerase Chain ◽

The Relationship

AbstractAt a commercial farrow-to-feeder pig system with 2,100 sows in Serbia, lesions resembling porcine ear necrosis syndrome were observed in 80% of the weaned pigs at 45–50 days of age. Pathomorphological examinations were carried out on 10 pigs that had been found dead. The gross lesions ranged from mild, superficial dermatitis to severe, deep inflammation with exudation, ulceration and necrosis. Histopathological examination revealed erosive and ulcerative dermatitis of the pinna with neutrophilic and lymphocytic infiltration and bacterial colonies in the crusts. Staphylococcus aureus (MRSA strain), Staphylococcus hyicus and Streptococcus group C were cultivated from eight, S. hyicus from two ear tissue scraping samples. All 10 samples were positive for treponemes and phylogenetic analysis of two polymerase chain reaction products confirmed the relationship to Treponema (T.) medium/vincentii and Treponema pedis. Treponemes were also detected in seven oral swabs that were analysed to obtain evidence of the transmission of this bacterium by ear biting. The contribution of non-infectious factors to this misbehaviour could not be ruled out as the crude protein concentration of the feed was inappropriate and the climate of the pig house was suboptimal. The concentrations of selected mycotoxins in the feed were not elevated. However, the contribution of both infectious and non-infectious factors to the onset of disease was most probable.

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Analysis of Helicobacter pylori Prevalence in Chittagong, Bangladesh, Based on PCR and CLO Test

Microbiology Insights ◽

10.4137/mbi.s39858 ◽

2016 ◽

Vol 9 ◽

pp. MBI.S39858 ◽

Cited By ~ 2

Author(s):

Abdul Musaweer Habib ◽

Jibran Alam ◽

Bashudev Rudra ◽

Abdul Quader ◽

Mohammad Al-Forkan

Keyword(s):

Helicobacter Pylori ◽

Current Trend ◽

Chain Reaction ◽

Gastric Diseases ◽

Age And Gender ◽

Ulcer Disease ◽

Specific Polymerase Chain Reaction ◽

Polymerase Chain ◽

And Gender ◽

H Pylori

The pathogenic bacterium Helicobacter pylori is a causative agent of gastric diseases in Bangladesh as well as throughout the world. This study aimed at analyzing the prevalence of H. pylori infection among dyspeptic patients in Chittagong, the second most populous city of Bangladesh, using 16S rRNA-based H. pylori-specific Polymerase Chain Reaction and Campylobacter-like organism test. We found that 67% of the population under study was positive for H. pylori infection. Gastric ulcer and duodenal ulcer disease showed statistically significant association with H. pylori infection; however, no association of H. pylori infection was observed in terms of age and gender. This study would play a crucial role in managing H. pylori-induced gastric diseases by understanding the current trend of H. pylori infection in the Chittagong region of Bangladesh.

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Analysis of Helicobacter pylori Genotypes in Afghani and Iranian Isolates

Polish Journal of Microbiology ◽

10.33073/pjm-2010-009 ◽

2010 ◽

Vol 59 (1) ◽

pp. 61-66 ◽

Cited By ~ 8

Author(s):

HOSSEIN DABIRI ◽

MEHDI BOLFION ◽

AKBAR MIRSALEHIAN ◽

MARYAM REZADEHBASHI ◽

FERESHTEH JAFARI ◽

...

Keyword(s):

Helicobacter Pylori ◽

Peptic Ulcer ◽

Clinical Outcomes ◽

Positive Culture ◽

Chain Reaction ◽

Vacuolating Cytotoxin ◽

Ulcer Disease ◽

Polymerase Chain ◽

H Pylori ◽

Iranian Patients

The geographical variation in Helicobacter pylori genotypes is an observed phenomenon. Cytotoxin associated genes A (cagA) and E (cagE), and vacuolating cytotoxin (vacA) genotypes of H. pylori are associated with peptic ulcer disease (PUD). This study compared the distribution of these genotypes in Iranian and Afghani isolates and their association with clinical outcomes. H. pylori infected patients, as proven by positive culture, were recruited prospectively. A total of 70 patients, 55 Iranian (26 men and 29 women, mean age 48 +/- 18 years) and 15 Afghani immigrants (13 men and 2 women, mean age 34.8 +/- 11 years) living in Tehran, Iran were enrolled in this study. DNA was extracted from isolated H. pylori and polymerase chain reaction was carried out to determine the cagA and cagE status and vacA alleles. The number of gastric cancer, peptic ulcer and gastritis cases was 11, 23 and 36, respectively. The cagA positive isolates were more common in Iranian (67%) than Afghani isolates (60%). cagE was positive in 53% of Afghani compared to 51% of Iranian isolates. The most common vacA s-region genotype was s1; 80% in Afghani and 67% in Iranian. The slml was a frequently observed genotype in Afghani strains (53%) while s1m2 (47%) was more common in strains isolated from Iranian patients. There is a difference in the H. pylori strains between Iranian and Afghani groups, for instance Iranian isolates were similar to European isolates while Afghani isolates were similar to isolates from India. However, there was no significant association between cagA, cagE and vacA genotypes and clinical outcomes in Iranian and Afghani patients.

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cagA-Seropositive Strains ofHelicobacter pyloriIncrease the Risk for Gastric Cancer more than the Presence ofH pyloriAlone

Canadian Journal of Gastroenterology ◽

10.1155/2004/424018 ◽

2004 ◽

Vol 18 (5) ◽

pp. 341-343

Author(s):

Naoki Chiba

Keyword(s):

Gastric Cancer ◽

Odds Ratio ◽

Meta Analysis ◽

Review Of The Literature ◽

Chain Reaction ◽

Positive Strain ◽

Increased Risk ◽

Polymerase Chain ◽

H Pylori ◽

The Relationship

Huang et al have performed a meta-analysis to determine the relationship betweencagA seropositivity (by serology and polymerase chain reaction) and the risk of gastric cancer. An extensive review of the literature identified no previous systematic overviews. The authors identified 16 studies involving 2284 cases and 2770 controls. The overall prevalence of Helicobacter pylori was 77.7% in cases and 63.1% in controls. Tests forcagA were positive in 62.8% of cases and 37.5% of controls. Thus,H pyloriandcagA seropositivity significantly increased the risk for gastric cancer, by 2.28 (95% CI 1.71 to 3.05) and 2.87 (95% CI 1.95 to 4.22), respectively. In patients withH pylori, those who were infected by acagA-positive strain had a slightly higher risk of gastric cancer, with an odds ratio of 1.64 (95% CI 1.21 to 2.24). The authors also found that patients infected withH pyloriwith or withoutcagA seropositivity had an increased risk of noncardia gastric cancer, but not of cancer of the gastric cardia. They concluded thatcagA-positive strains confer a greater risk of gastric cancer than does H pylori infection alone.

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Geographic Distribution of The cagA, vacA, iceA, oipA and dupA Genes of Helicobacter Pylori Strains Isolated in China

10.21203/rs.3.rs-136079/v1 ◽

2020 ◽

Author(s):

Zhijing Xue ◽

Hong Yang ◽

Dongxing Su ◽

Xiangfeng Song ◽

Xin Deng ◽

...

Keyword(s):

Helicobacter Pylori ◽

Regional Distribution ◽

Chain Reaction ◽

Geographical Differences ◽

Biopsy Specimens ◽

Geographic Differences ◽

Polymerase Chain ◽

The Difference ◽

H Pylori ◽

Geographical Characteristics

Abstract Background: There were geographical differences in the distribution of Helicobacter pylori (H. pylori) genotypes (cagA, vacA, iceA, oipA and dupA, et al). The population in different regions in China have grant different patterns of gastroduodenal diseases which are associated with these genotypes, but the geographical characteristics of H. pylori genotypes were still unknown. Materials and Methods: Gastric biopsy specimens were obtained from 348 patients from five regions in China. The regional distribution was 89 patients from Shandong, 91 from Guangxi, 57 from Hunan, 58 from Qinghai and 53 from Heilongjiang. DNA extracted from cultured isolates were analyzed by polymerase chain reaction (PCR) to determine the presence of cagA, vacA, iceA, oipA and dupA genotypes.Results: A total of 269 H. pylori isolates were obtained, of which 74 isolates were from Shandong, 78 from Guangxi, 46 from Hunan, 33 from Qinghai and 38 from Heilongjiang. The cagA gene was predominant in all the five regions (e.g. 100% in Hunan, Qinghai and Heilongjiang). The predominant vacA genotypes in the 269 isolates were s1a (88.1%) and m1(72.1%). vacA s1b was not detected in our study. In strains from Guangxi and Hunan, s1c was dominant; in contrast, s1a was dominant in Shandong, Qinghai and Heilongjiang. The prevalence of m1 strains in Heilongjiang (92.1%) was significantly higher (P<0.001) than in Shandong (60.8%) and Qinghai (51.5%). The dominant vacA subtype combination was s1a/m1 (62.8%) and detection of vacA s1a/m1 was significantly high 34 (89.5%) in Heilongjiang strains (P<0.001). The prevalence of iceA alleles in Hunan and Qinghai was much higher than that in the other three regions, and the difference was statistically significant. The oipA-positive strains were more prevalent in Guangxi (100%) and Hunan (100%) than in Qinghai (78.8%) (P<0.001). Conversely, the dupA-positive strains were less than half in Guangxi (15.4%) and Shandong (32.4%), whereas it was 73.9% in Hunan and 81.8% in Qinghai (P<0.001).Conclusions: There are significant geographic differences in the distribution of H. pylori genotypes. These datas may be used to explain the gastroduodenal diseases patterns in different geographic regions of China.

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Diagnosis and posttreatment follow-up of Helicobacter pylori-positive gastric lymphoma of mucosa-associated lymphoid tissue: histology, polymerase chain reaction, or both?

Blood ◽

10.1182/blood.v87.4.1255.bloodjournal8741255 ◽

1996 ◽

Vol 87 (4) ◽

pp. 1255-1260 ◽

Cited By ~ 147

Author(s):

A Savio ◽

G Franzin ◽

AC Wotherspoon ◽

G Zamboni ◽

R Negrini ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

B Cell ◽

Lymphoid Tissue ◽

Gastric Lymphoma ◽

Cell Populations ◽

Chain Reaction ◽

Clonal Population ◽

Biopsy Specimens ◽

Polymerase Chain ◽

H Pylori

The differential diagnosis between Helicobacter pylori (H pylori- associated chronic gastritis and low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue (MALT) and the assessment of endoscopic biopsy specimens after treatment of lymphoma can be problematic. Although immunocytochemistry can be used to identify clonal B-cell populations, which are characteristic of MALT lymphoma, its application to small biopsy specimens and the subsequent interpretation can be difficult. The polymerase chain reaction (PCR) can detect clonal B-cell populations by analysis of the Ig heavy chain gene in routinely fixed paraffin-embedded material and might provide a useful tool in the assessment of these specimens. We have investigated the value of histology and PCR in the diagnosis of lymphoma and its followup in formalin-fixed paraffin-embedded gastric endoscopy biopsy specimens from 69 sequential patients selected on the basis of a dense mucosal lymphoid infiltrate associated with H pylori infection. Histologic evidence of MALT lymphoma was identified in 13 cases, 9 of which showed PCR-detected monoclonality. In 12 of 13 cases, H pylori was eradicated, and in 11 of 12 cases, histologic regression of the lymphoma followed. PCR evidence of monoclonality disappeared in 6 of 9 originally monoclonal cases. This was synchronous with histologic remission in 1 case, but lagged in the remaining 5 cases by up to 28 months. Two of the 3 of the 9 cases originally monoclonal by PCR that have not shown molecular regression have monoclonal-amplified products 17 and 24 months after negative histology. In 3 cases, the histology of the biopsies was considered indeterminate or discordant. In 1 of these cases, the histologic features were obscured by crush artefact. In a second case, there was molecular evidence of monoclonality in the absence of histologic features suggestive of lymphoma; this persisted after H pylori eradication. An additional single case originally diagnosed as reactive developed a PCR detectable clonal population 29 months after original evaluation in the absence of histologic features of lymphoma but in the presence of persistent H pylori infection. These findings suggest that the histologic assessment of gastric biopsies remains the method of choice for the diagnosis of lymphoma in gastric endoscopic biopsies with a dense mucosal lymphoid infiltrate. PCR provides a useful technique to support the diagnosis if clonal amplification products are found. The significance of PCR detected clonality in the absence of histologic evidence of lymphoma in uncertain but may represent a stage of tumor progression/regression when the clonal population is insufficient to be detected by conventional histology. This is supported by the evidence that PCR- detectable monoclonality can persist after treatment and the disappearance of histologically detectable lymphoma.

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Molecular Identification of Helicobacter pylori and IceA Genes Frequency from Dental Plaques Isolated from People Using PCR Method

International Journal of Medical Laboratory ◽

10.18502/ijml.v7i3.4137 ◽

2020 ◽

Author(s):

Zahra Salari ◽

Atefeh Ranjkesh ◽

Emad Behboudi

Keyword(s):

Polymerase Chain Reaction ◽

Helicobacter Pylori ◽

Chain Reaction ◽

Ulcer Disease ◽

Two Samples ◽

Pcr Method ◽

Dental Plaques ◽

Polymerase Chain ◽

H Pylori ◽

Development And Evolution

Background and Aims: Helicobacter pylori (H. pylori) is a gram-negative, microaerophilic, spiral-shaped flagellated bacterium that is urease, catalase and oxidase positive. One of its pathogenicity factors is the iceA gene. H. pylori has recently been recognized as a genetic indicator for the development and evolution of duodenal ulcer disease in the East. This study aimed to determine the presence of this bacterium in gingival plaques in non-endocrine patients in Bojnourd city, and the polymerase chain reaction technique examined the percentage of iceA gene. Materials and Methods: A total of 100 samples of dental plaque were taken and transferred to a tube that has been physiologically placed. After DNA extraction, primer design was performed, and then the polymerase chain reaction was performed for the whole sample. Results: Of 100 samples examined in this study, two samples of H. pylori were positive (2%), and the frequency of the iceA gene of two samples was positive (100%). Conclusion: In the Bojnord city, the frequency of iceA gene in people is high, and the frequency of H. pylori in tooth plaques is low. Also, iceA gene can be considered as an indicator for predicting the contamination and risk of H. pylori infection in the region. To confirm the results, more molecular studies are required in other populations.

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Direct detection of Helicobacter pylori resistance to macrolides by a polymerase chain reaction/DNA enzyme immunoassay in gastric biopsy specimens

Gut ◽

10.1136/gut.44.4.463 ◽

1999 ◽

Vol 44 (4) ◽

pp. 463-467 ◽

Cited By ~ 65

Author(s):

A Marais ◽

L Monteiro ◽

A Occhialini ◽

M Pina ◽

H Lamouliatte ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Gastric Biopsy ◽

23S Rrna ◽

Wild Type ◽

Chain Reaction ◽

Biopsy Specimens ◽

Resistant Strains ◽

Polymerase Chain ◽

H Pylori

BACKGROUNDThe increasing use of macrolides especially in the treatment ofHelicobacter pylori infection has led to an increase in resistant strains. The resistance of H pylori to macrolides, especially clarithromycin, is one of the major causes of eradication failure. In H pylori, clarithromycin resistance is due to point mutations localised in domain V of 23S rRNA.AIMTo develop a molecular technique based on amplification of a relevant fragment of the 23S rRNA and colorimetric hybridisation in liquid phase to detect directly in biopsy specimens the type of mutation associated with resistance of H pylori to clarithromycin.METHODSGastric biopsy samples from 61 patients were submitted to this test. The results were compared with standard methods (determination of minimal inhibition concentration, polymerase chain reaction/restriction fragment length polymorphism, and/or DNA sequencing) in order to evaluate the test and to define the cut off values, specificity, and sensitivity.RESULTSThe 14 biopsy samples in which H pylori was not detected did not give a positive result in any assay, and the 14 samples harbouring strains susceptible to clarithromycin gave a positive result with the wild type probe as expected. The 33 biopsy specimens containing resistant strains always gave a positive signal with one of the probes detecting resistant organisms, but in eight cases they also reacted with the wild type probe, indicating that a mixture of resistant and susceptible organisms was present.CONCLUSIONThe importance of this new assay is that it allows the detection of multiple genotypes corresponding to either heterogeneous genotypes or mixed infections. Moreover, it allows in a single step not only the detection of H pylori but also the determination of its susceptibility to clarithromycin directly in biopsy specimens without the need for culture.

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Zebrafish embryos exposed to deltamethrin exhibit abnormalities despite induced expression of related genes (you, you-too, momo and u-boot)

Toxicology and Industrial Health ◽

10.1177/0748233718807046 ◽

2018 ◽

Vol 35 (1) ◽

pp. 11-19

Author(s):

Kuder Reshma Shabnam ◽

Dharmapuri Gangappa ◽

Gundala Harold Philip

Keyword(s):

Vital Role ◽

Primary Objective ◽

Chain Reaction ◽

Environmental Persistence ◽

Expression Of Genes ◽

Pericardial Edema ◽

Eye Pigmentation ◽

Polymerase Chain ◽

Relationship Of ◽

The Relationship

Evaluation of the toxic effects of a widely used synthetic pyrethroid, deltamethrin (DM), was carried out in this study. This pesticide is preferred for pest control because of its low environmental persistence and toxicity. We investigated the expression pattern of four genes, namely, you ( you), yot ( you-too), momo ( mom) and ubo ( u-boot) during early development of zebrafish, that is, from 12 hpf to 48 hpf stages. These stages are selected as most of the important developmental aspects take place during this period. All four genes are known to play a vital role in development of notochord and somites. To understand the effect of DM on development, embryos of 4 hpf stage were exposed to two concentrations (100 and 200 µg/L) of DM, and observations were made at 12, 24 and 48 hpf stages. Our earlier studies have shown phenotypic abnormalities such as notochord bending, tail deformation, yolk sac and pericardial edema, lightening of body and eye pigmentation and interfered in somite patterning, during these stages of development. Understanding the relationship of phenotypic abnormalities with these four genes has been our primary objective. These four genes were analyzed by Reverse transcription (RT)-polymerase chain reaction and intensity of the bands has shown induction in their expression after exposure to the toxicant. In spite of the expression of genes, it was noticed that DM caused abnormalities. It can be said from the results that translational pathway could have been affected.

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