Chemical Compositions, Antioxidant Activities, and Mineral Matter Contents of Achillea collina Becker ex Rchb from the Flora of Bulgaria

Aim and Objective: Oxidative stress is implicated in the development and progression of many disease. Some of appropriate actions that could be initiated to taken to resolve the problem of these diseases are search for new antioxidant substances isolated from plants. The aims of this study were to study the intraspecies variations of A. verticillata and C. caeruleus essential oils from 8 locations using statistical analysis, the in vitro antioxidant properties of collective essential oils and in combinations. Materials and Methods: The essential oils were analyzed by GC and GC-MS. The intraspecies variations of the essential oil compositions were discussed using principal component analysis (PCA) and cluster analysis (CA). The antioxidant properties were evaluated DPPH-radical scavenging activity and β-carotene bleaching test. Results: The main components of Ammoides verticillata collective essential oil (Coll EO) were thymol (30.5%), carvacrol (23.2%), p-cymene (13.1%), limonene (12.5%) and terpinene-4-ol (12.3%). While roots of Carthamus caeruleus essential oil were dominated by carline oxide (86.2%). The chemical variability allowed the discrimination of two main Groups for both Coll EOs. A direct correlation between the altitudes, climate and the chemical compositions was evidenced. Ammoides verticulata and Carthamus caeruleus Coll Eos showed good antioxidant activity. In binary mixture, the interaction both Coll Eos and between oils rich of thymol and/or carvacrol with carlina oxide produced the best synergistic effects, compared to individual essential oils and the synthetic antioxidant (BHT). Conclusion: Ammoides verticillata and Carthamus caeruleus essential oil blends can be used as a natural food preservative and alternative to chemical antioxidants.

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Optimization of Ultrasonic-Assisted Extraction, Characterization, and Antioxidant Activities of Polysaccharides From Sojae Semen Praeparatum

Natural Product Communications ◽

10.1177/1934578x211020622 ◽

2021 ◽

Vol 16 (5) ◽

pp. 1934578X2110206

Author(s):

Yongshuai Jing ◽

Ruijuan Zhang ◽

Lan Li ◽

Danshen Zhang ◽

Yu Liu ◽

...

Keyword(s):

Antioxidant Activities ◽

Deae Cellulose ◽

Chemical Compositions ◽

Hydroxyl Groups ◽

Ultrasonic Frequency ◽

Ultrasonic Power ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

In this study, response surface methodology (RSM) was used to optimize the ultrasonic-assisted extraction parameters of Sojae Semen Praeparatum polysaccharides (SSPP-80), the optimum conditions were determined as follows: ultrasonic frequency of 100 W, ultrasonic power of 80 Hz, ultrasonic temperature of 52℃, ultrasonic time of 23 minutes, and liquid to raw material ratio of 40 mL/g. Based on these conditions, polysaccharides extraction rate was 7.72% ± 0.26%. Then, 2 novel polysaccharides (SSPP-80‐1, SSPP-80‐2) were isolated from SSPP by DEAE-cellulose 52 chromatography. The chemical compositions, physicochemical properties, and structure of SSPPs were investigated by simultaneous thermal analyzer (TGA), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FI-IR), and high-performance liquid chromatography (HPLC). The results showed that SSPP-80 and 2 fractions were mainly composed of mannose (Man), glucose (Glc), galactose (Gal), xylose (Xyl), and arabinose (Ara). In addition, the antioxidant activities were evaluated against the DPPH and hydroxyl radical in vitro, the IC50 of SSPP-80, SSPP-80‐1 and SSPP-80‐2 against DPPH free radical were 4.407, 8.267, and 5.204 mg/mL, respectively, whereas the IC50values for removing hydroxyl groups were 5.318, 3.516, and 4.016 mg/mL, respectively. It demonstrated that SSPP-80 and 2 fractions had certain antioxidant activity. Theoretical basis for use of Sojae Semen Praeparatum polysaccharides was provided by this study.

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Antioxidant and Cytotoxic Activities of Myrtaceae Essential Oils Rich in Terpenoids From Brazil

Natural Product Communications ◽

10.1177/1934578x21996156 ◽

2021 ◽

Vol 16 (2) ◽

pp. 1934578X2199615

Author(s):

Lucas Botelho Jerônimo ◽

Jamile S. da Costa ◽

Laine C. Pinto ◽

Raquel C. Montenegro ◽

William N. Setzer ◽

...

Keyword(s):

Essential Oils ◽

Cancer Cells ◽

Antioxidant Activities ◽

Human Cancer ◽

Psidium Guajava ◽

Cytotoxic Activities ◽

Chemical Compositions ◽

Main Compound ◽

Human Cancer Cells ◽

High Concentrations

This work analyzed the chemical compositions and evaluated the antioxidant and cytotoxic activities of essential oils (EO) of Eugenia patrisii (Epat), Eugenia stipitata (Esti), Myrcia splendens (Mspl), Myrcia sylvatica (Msyl), Psidium guajava (Pgua), and Psidium guineense (Pgui-1 and Pgui-2) from the Brazilian Amazon. Sesquiterpenoids were found in high concentrations in the oils of E. patrisii and M. splendens, which were rich in E-caryophyllene (32.0% and 45.8%); E. stipitata and M. sylvatica, which displayed germacrene D (11.8%) and germacrene B (24.5%); and P. guajava that showed epi-β-bisabolol (16.1%) as the main compound. However, P. guineense samples (Pgui-1 and Pgui-2) were rich in monoterpenoids such as limonene (Pgui-1: 30.2%; Pgui-2 30.4%) and α-pinene (Pgui-1: 22.5%; Pgui-2: 17.7%). The samples showed a weak and moderate antioxidant activities in the DPPH assay, displaying inhibition rates from 11.5% to 38.6% (at 10 mg/mL). All samples were cytotoxic against human cancer cells by the MTT method. Epat oil showed higher activity against melanoma (SKMEL-19, IC505.8 µg/mL), gastric (AGP01, IC503.2 µg/mL), and colon (HCT116, IC506.7 µg/mL). Meanwhile, the samples Pgua and Pgui were more active against breast cancer cells (MCF7, IC5012.4 µg/mL and 11.6 µg/mL, respectively).

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Insights Into Antioxidant Activities and Anti-Skin-Aging Potential of Callus Extract From Centella Asiatica (L.)

10.21203/rs.3.rs-218867/v1 ◽

2021 ◽

Author(s):

Visarut Buranasudja ◽

Dolly Rani ◽

Ashwini Malla ◽

Khwanlada Kobtrakul ◽

Sornkanok Vimolmangkang

Keyword(s):

Oxidative Stress ◽

Callus Culture ◽

Antioxidant Activities ◽

Centella Asiatica ◽

Skin Aging ◽

Dermal Fibroblasts ◽

Bioactive Metabolites ◽

Chemical Components ◽

Chemical Compositions ◽

Scale Production

Abstract Formation of oxidative stress in dermal fibroblasts plays crucial roles in aging processes of skin. The use of phytochemicals that can promote capacity of fibroblasts to combat oxidative stress is an attractive strategy to prevent skin aging and promote skin beauty. Callus culture offers a powerful platform for sustainable, rapid and large-scale production of phytochemicals to serve extensive demands of pharmaceutical and cosmeceutical industries. Here, we demonstrated the application of callus culture of Centella asiatica to produce bioactive metabolites. The 50% ethanolic extract of callus culture has distinctive features of chemical compositions and biological profiles. Results from TLC and HPLC analysis showed that callus extract has unique chemical components, compared with those isolated from authentic plant. Moreover, callus extract possesses promising antioxidant and anti-skin-aging activities. Pre-treatment with callus extract attenuated H2O2-induced-cytotoxicity on human dermal fibroblasts. The results from RT-qPCR clearly suggested that the upregulation of cellular antioxidant enzymes appeared to be major contributor for the protective effects of callus extract against oxidative stress. Moreover, supplementation with callus extract inhibited induction of matrix metalloprotease-9 following H2O2 exposure, suggesting its potential anti-skin-aging activity. Our results demonstrate the potential utility of C. asiatica callus extract as anti-skin-aging agent in cosmeceutical preparations.

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Chemical profiling and antioxidant activities of essential oil from rhizomes of Acorus calamus L.

BIBECHANA ◽

10.3126/bibechana.v17i0.25201 ◽

2020 ◽

Vol 17 ◽

pp. 89-95

Author(s):

Sumnath Khanal ◽

Devi Prasad Bhandari ◽

Laxman Bhandari ◽

Achyut Adhikari

Keyword(s):

Essential Oil ◽

Antioxidant Activities ◽

Gas Chromatography Mass Spectrometry ◽

Acorus Calamus ◽

Chemical Compositions ◽

Chemical Profiling ◽

Ic50 Value ◽

Sweet Flag ◽

Dpph Scavenging ◽

Dpph Scavenging Assay

Background and Aims: Acorus calamus L. is an indigenous herb in Nepal. It belongs to family Acoraceae and grows in wet land with scented rhizomes. It is also known as Sweet flag in English and commonly as Bojho in Nepal. The present investigation reveals the chemical compositions and antioxidant activity of rhizome essential oil of A. calamus. Methods: Essential oil of rhizomes of Acorus calamus L. from Kaski district, Nepal was extracted by hydrodistillation method and volatile constituents were analyzed Gas chromatography-Mass spectrometry. The antioxidant potential of essential oil was analyzed by 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) scavenging assay. Results: A GC-MS analysis revealed the presence of β-asarone (22.38%), α-asarone (14.97%), 1-(4,6-dimethoxy-2,3-dimethylphenyl ethanone (14.24%), Isoelemicin (5.68%), cis-Methylisoeugenol (4.26%), α-calacorene (4.16%), and other 20 minor components. From DPPH assay, half maximal inhibitory concentration (IC50) value of essential oil was found to be 108.71 µg/mL. Conclusions: These findings have strengthened the A. calamus L. is good source of compounds like β-asarone, α-asarone and can be used as potential antioxidants. BIBECHANA 17 (2020) 89-95

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Studies on Chemical Compositions, Antimicrobial and Antioxidant Activities of Essential Oils ofSalvia officinialisLinn. Grown in Two Locations of Ethiopia

Journal of Essential Oil Bearing Plants ◽

10.1080/0972060x.2017.1287008 ◽

2017 ◽

Vol 20 (2) ◽

pp. 426-437 ◽

Cited By ~ 1

Author(s):

Gelila Asamenew ◽

Kaleab Asres ◽

Daniel Bisrat ◽

Avijit Mazumder ◽

Peter Lindemann

Keyword(s):

Essential Oils ◽

Antioxidant Activities ◽

Chemical Compositions

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Comparative Study of the Chemical Compositions and Antioxidant Activities of Fresh Juices from Romanian Cucurbitaceae Varieties

Molecules ◽

10.3390/molecules25225468 ◽

2020 ◽

Vol 25 (22) ◽

pp. 5468

Author(s):

Anna Cazanevscaia Busuioc ◽

Andreea-Veronica Dediu Botezatu ◽

Bianca Furdui ◽

Costel Vinatoru ◽

Filippo Maggi ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

High Performance ◽

Antioxidant Activities ◽

Fruit Juices ◽

Antioxidant Potential ◽

Chemical Compositions ◽

Beneficial Effects ◽

Benincasa Hispida ◽

First Time

Cucurbitaceae is a family of health-promoting plants due to their compounds with beneficial effects. The aim of this study was to analyze, for the first time, the chemical composition, the antioxidant activity and the metal chelating properties of fruit juices obtained from four different species of the Cucurbitaceae family cultivated in Romania, namely Momordica charantia, Cucumis metuliferus, Benincasa hispida and Trichosanthes cucumerina. The samples of juice were analyzed by high-performance liquid chromatography (HPLC) and all the four species displayed high levels of the two triterpenes, oleanolic and ursolic acids, and also in phenolic compounds, including catechin, (−)-epicatechin and gallic acid. The juices demonstrated significant antioxidant activity against the free radical 2,2-diphenyl-1-picrylhydrazyl (ranging from 20 to 95%,), a good iron binding ability (ranging from 7.45 ± 0.28% to 86.95 ± 0.97%) and also promising antioxidant potential against the ABTS radical (ranging from 4.97 to 32.60 μETx/mL juice). Our findings raise interesting questions for further research on Cucurbitaceae fruit juices and, consequently, their very good antioxidant potential suggests these fruits should be further explored for their protective effect against oxidative damage. This is the first time the chemical composition and antioxidant activities of fruit juices from these four Romanian Cucurbitaceae varieties have been investigated.

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Evaluation of Antioxidant and Anti-α-glucosidase Activities of Various Solvent Extracts and Major Bioactive Components from the Seeds of Myristica fragrans

Molecules ◽

10.3390/molecules25215198 ◽

2020 ◽

Vol 25 (21) ◽

pp. 5198

Author(s):

Cai-Wei Li ◽

Yi-Cheng Chu ◽

Chun-Yi Huang ◽

Shu-Ling Fu ◽

Jih-Jung Chen

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Significant Inhibition ◽

Chemical Compositions ◽

Bioactive Components ◽

Active Components ◽

Myristica Fragrans ◽

Ic50 Values ◽

Acetone Extracts

Myristica fragrans is a well-known species for flavoring many food products and for formulation of perfume and medicated balm. It is also used to treat indigestion, stomach ulcers, liver disorders, and, as emmenagogue, diaphoretic, diuretic, nervine, and aphrodisiac. We examined antioxidant properties and bioactive compounds in various solvent extracts from the seeds of M. fragrans. Methanol, ethanol, and acetone extracts exhibited relatively strong antioxidant activities by 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide radical, and hydroxyl radical scavenging tests. Furthermore, methanol extracts also displayed significant anti-α-glucosidase activity. Examined and compared to the various solvent extracts for their chemical compositions using HPLC analysis, we isolated the ten higher content compounds and analyzed antioxidant and anti-α-glucosidase activities. Among the isolates, dehydrodiisoeugenol, malabaricone B and malabaricone C were main antioxidant components in seeds of M. fragrans. Malabaricone C exhibited stronger antioxidant capacities than others based on lower half inhibitory concentration (IC50) values in DPPH and ABTS radical scavenging assays, and it also showed significant inhibition of α-glucosidase. These results shown that methanol was found to be the most efficient solvent for extracting the active components from the seeds of M. fragrans, and this material is a potential good source of natural antioxidant and α-glucosidase inhibitor.

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