scholarly journals Phenolic profiles and antioxidant activities of exocarp, endocarp, and hypanthium of three pear cultivars grown in China

2021 ◽  
Vol 14 ◽  
Author(s):  
Bin Du ◽  
Caihong Cheng ◽  
Yuqin Chen ◽  
Jinhui Wu ◽  
Fengmei Zhu ◽  
...  

The phenolic composition of the exocarp, hypanthium, and endocarp from three pear cultivars: Jingbai, Korla and Crystal were measured by high-performance liquid chromatography method with diode array detection (HPLC-DAD), while antioxidant activities were investigated using DPPH radical andABTS radical cation scavenging activity, and FRAP. Ferulic acid, chlorogenic acid, and caffeic acid were detected as major components. The exocarp of the Jingbai pear had a relatively high chlorogenic acid content (0.691 mg kg-1) while the hypanthium and endocarp had the high chlorogenic acid content of the three pear varieties. The exocarp of the Korla pear had the highest ferulic acid and rutin content. The exocarp, hypanthium, and endocarp of the Crystal pear had the highest content of chlorogenic acid of the three pear varieties. The Crystal pear presented the highest total phenolic and flavonoid contents and had the highest antioxidant activities in terms of FRAP. The Jingbai pear exocarp presented the highest ABTS value, 83.62 ± 0.08%. The endocarp of the Crystal pear had the highest DPPH value, 87.30 ± 0.03%. Positive correlations were detected among chlorogenic acid, TPC, TFC, and FRAP. The results reveal that the different parts of Pyrus communis L. have effective antioxidant activity for the pharmaceutical application.

2021 ◽  
Vol 5 ◽  
Author(s):  
Seda Kayahan ◽  
Didem Saloglu

The objective of this work was to determine the total phenolic compounds and antioxidants in raw and cooked Sakiz and Bayrampasa variety artichokes in parts such as inner bracts, stems, receptacles, and outer bracts. The artichokes were cooked by boiling, microwaving, and baking methods, and total phenolic compounds and antioxidants of cooked artichokes were evaluated. While TPC (total phenolic content), DPPH (2,2-diphenyl-1-picryl-hydrazine), and CUPRAC values for the leaves of raw Bayrampasa artichoke were found to be 686 mg gallic acid equivalent (GAE)/100 g, 478 mg TE/100 g, and 4,875 mg TE/100 g, respectively, TPC, DPPH, and CUPRAC values for stems of Sakiz artichoke were determined to be 1,579 mg GAE/100 g, 1,259 mg TE/100 g, and 3,575 mg TE/100 g. A significant increase in the content of TPC, DPPH, and CUPRAC values was observed for all cooking applications of both artichokes. DPPH and CUPRAC values increased by 11 and 43 times and 17 and 6.7 times after baking of Sakiz and microwave cooking of Bayrampasa, respectively. Chlorogenic acid, cynarine, and cynaroside content of both artichokes had an increment after all cooking applications. Chlorogenic acid content was improved 29 and 58 times after baking of Sakiz and microwave cooking of Bayrampasa, respectively.


2020 ◽  
Vol 66 (No. 2) ◽  
pp. 57-64 ◽  
Author(s):  
Luboš Paznocht ◽  
Zora Kotíková ◽  
Barbora Burešová ◽  
Jaromír Lachman ◽  
Petr Martinek

The content of free, conjugated and bound phenolic acids in 12 wheat (Triticum aestivum L.) genotypes of 4 different grain colours (standard red, yellow endosperm, purple pericarp and blue aleurone) from 2-year field trial was analysed in the present study. Significant increase (8%) in the total phenolic acid content was observed in the dryer year 2017. Five phenolic acids (ferulic, sinapic, p-coumaric, vanillic and 4-hydroxybenzoic) and cis-isomers of ferulic and sinapic acid were determined by HPLC-DAD (high-performance liquid chromatography with a diode-array detector) in grain samples. The total phenolic acid content of coloured wheat groups varied: blue aleurone > purple pericarp > yellow endosperm > red colour (798 > 702 > 693 > 599 µg/g). The fraction of bound phenolic acids was the major contributor to the total phenolic acid content (91.7%) with ferulic acid predominating (85.2%). Conjugated phenolic acids accounted for 7.9% of the total with sinapic and ferulic acid predominating (47.6% and 19.9%). The composition of individual phenolic acids was similar within these two fractions. The remaining 0.4% was represented by the fraction of free phenolic acids in which the phenolic acid profile varied among the individual coloured groups. Ferulic acid prevailed in red and yellow wheats, vanillic in blue and p-coumaric in purple wheats.  


Author(s):  
O. V. Krivoruchko

Introduction. Sorbus domestіca and Sorbus graeca from the Rosaceae family are cultivated in Ukraine in gardens and parks. Their chemical composition is investigated insufficiently.The aim of the study – to research the composition and the content of phenolic compounds of leaves of Sorbus domestіca and Sorbus graeca.Research Methods. The content of phenolic compounds in leaves of Sorbus domestіca and Sorbus graeca was carried out by the high performance liquid chromatography method on Agilent Technologies chromatograph.Results and Discussion. In leaves of Sorbus domestіca chlorogenic acid, rutin, quercetin-3-O-glucoside and quercetin were identified; in leaves of Sorbus graeca chlorogenic and neochlorogenic acids, rutin and 4'-methoxyquercetin-3-O-sophoroside were identified. The content of hydroxycinnamic acids in leaves of Sorbus domestіca is 389 mg/100 g, in leaves of Sorbus graeca – 147 mg/100 g (in terms of chlorogenic acid). The content of flavonoids in leaves of Sorbus domestіca is 1888 mg/100 g, in leaves of Sorbus graeca – 727 mg/100 g (in terms of rutin).Conclusions. The composition and the content of phenolic compounds of Sorbus domestіca and Sorbus graeca were studied by the HPLC method. Leaves of Sorbus domestіca are more perspective for further pharmacological studies.


2020 ◽  
Vol 42 (2) ◽  
pp. 274-274
Author(s):  
Tuba Mert G nen Tuba Mert G nen ◽  
H sniye Kayalar and Munir zt rk H sniye Kayalar and Munir zt rk

Artichoke has been used for a long time in the traditional medicine against dyspeptic complaints and as a liver protector due to its choleretic and cholagogue effects. Chlorogenic acid, the main ingredient of artichoke is known to show hepatoprotector, anticancer, anti-obesity and antioxidant activities. In this study, antioxidant activity and chlorogenic acid quantification were performed using the samples of artichoke as fresh, boiled, frozen, boiled frozen, canned, fresh leaf and dry leaf. Potential antioxidant activity was evaluated using DPPH assay. Flavonoid and phenolic contents were determined by Aluminum chloride colorimetry and Folin-Ciocalteu methods. The amounts of colorogenic acid in the samples were determined by HPLC-DAD system. The results have shown that highest chlorogenic acid content was determined in the 10-minute frozen-boiled sample (0.01441 mg/100g), followed by canned (0.01043 mg/100g), 20-minute cooked artichoke (0.001912 mg/100g), fresh artichoke (0.001519 mg/100g) and frozen artichoke (0.0004 mg/100g). The antioxidant activity results have revealed that highest % DPPH capture effect was observed in the cooked artichoke sample (ED50 =1.949 mg/ml). The total flavonoid content was equivalent to quercetin (43.859 mg QE/100g) whereas total phenolic content equalled to gallic acid (14.640 mg GAE/100g). The antioxidant activity and the amounts of colorogenic acid were found to be the highest in the cooked artichoke sample whereas the lowest antioxidant activity observed in canned artichoke. The results show that the heat treatment applied for storage can positively affect the amount of chlorogenic acid and antioxidant activity.


2020 ◽  
Vol 12 (7) ◽  
pp. 2701 ◽  
Author(s):  
Nijolė Vaitkevičienė ◽  
Jurgita Kulaitienė ◽  
Elvyra Jarienė ◽  
Dovilė Levickienė ◽  
Honorata Danillčenko ◽  
...  

The purpose of this research is to evaluate the effect of conventional, biodynamic, and organic cultivation methods on the contents of polyphenols and carotenoids in tubers of potato cultivars with different colored flesh: “Red Emmalie”, “Salad Blue”, “Violetta”, “Tornado”, and “Laura”. These bioactive compounds were determined by the high-performance liquid chromatography method. The data received were analyzed with ANOVA and further explored and visualized by principal component analysis. Higher contents of polyphenols (sum), phenolic acids (sum), chlorogenic acid, p-coumaric acid, and caffeic acid were found in biodynamic and organic samples compared to the conventional tubers. Moreover, organically and biodynamically produced potatoes (except “Salad Blue” cultivar) were significantly richer in flavonoids and anthocyanins. The highest contents of carotenoids (sum), lutein, and β-carotene were found in biodynamic potatoes. Among the tested cultivars, “Tornado”, with white flesh, was richest in polyphenols (sum), phenolic acids (sum), chlorogenic acid, and p-coumaric acid. “Violetta”, with dark purple flesh, accumulated the highest contents of flavonoids (sum), anthocyanins (sum), petunidin-3,5-di-O-glucoside, pelargonidin-3,5-di-O-glucoside, and peonidin-3,5-di-O-glucoside. Carotenoids were only found in “Laura” tubers, and the dominating carotenoid was lutein.


Antioxidants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 47 ◽  
Author(s):  
Qiong-Qiong Yang ◽  
Lin Cheng ◽  
Zhi-Yuan Long ◽  
Hua-Bin Li ◽  
Anil Gunaratne ◽  
...  

Diverse peanut varieties are widely cultivated in China. However, few studies have investigated the effects of germination on the phenolic profiles and antioxidant activities of specific Chinese peanut cultivars. Therefore, this study was conducted to evaluate the effects of germination on total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, and phenolic profiles of seven peanut cultivars in China. The TPC, TFC, and antioxidant activities were determined by spectrophotometry, while phenolic profiles were analyzed by using ultra-high performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS). The results found that germination significantly increased TPC, TFC, and antioxidant activity. Antioxidant activity was found to be closely related to TPC in germinated peanut extracts, which indicates that phenolics are the main contributors of antioxidants in germinated peanuts. In addition, germination induced significant changes in polyphenolic profiles. In the analyzed samples, 36 phenolic compounds were identified in which most were flavonoids. Overall, these findings highlight that germinated peanuts can be a good natural source of natural antioxidants for human consumption and functional food development.


Sign in / Sign up

Export Citation Format

Share Document