scholarly journals Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MS

Antioxidants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 47 ◽  
Author(s):  
Qiong-Qiong Yang ◽  
Lin Cheng ◽  
Zhi-Yuan Long ◽  
Hua-Bin Li ◽  
Anil Gunaratne ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Human Consumption ◽  
Flavonoid Content ◽  
Flight Mass Spectrometry ◽  
Phenolic Profiles ◽  
Peanut Cultivars

Diverse peanut varieties are widely cultivated in China. However, few studies have investigated the effects of germination on the phenolic profiles and antioxidant activities of specific Chinese peanut cultivars. Therefore, this study was conducted to evaluate the effects of germination on total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, and phenolic profiles of seven peanut cultivars in China. The TPC, TFC, and antioxidant activities were determined by spectrophotometry, while phenolic profiles were analyzed by using ultra-high performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS). The results found that germination significantly increased TPC, TFC, and antioxidant activity. Antioxidant activity was found to be closely related to TPC in germinated peanut extracts, which indicates that phenolics are the main contributors of antioxidants in germinated peanuts. In addition, germination induced significant changes in polyphenolic profiles. In the analyzed samples, 36 phenolic compounds were identified in which most were flavonoids. Overall, these findings highlight that germinated peanuts can be a good natural source of natural antioxidants for human consumption and functional food development.

scholarly journals Establishing Relationship between Vitamins, Total Phenolic and Total Flavonoid Content and Antioxidant Activities in Various Honey Types

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4399
Author(s):  
Norhasnida Zawawi ◽  
Pei Juin Chong ◽  
Nurul Nadhirah Mohd Tom ◽  
Nurkhairina Solehah Saiful Anuar ◽  
Salma Malihah Mohammad ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Extrinsic Factors ◽  
Biochemical Compounds ◽  
Phenolic And Flavonoid

Honey is a well-known natural sweetener and is rich in natural antioxidants that prevent the occurrence of oxidative stress, which is responsible for many human diseases. Some of the biochemical compounds in honey that contribute to this property are vitamins and phenolic compounds such as phenolic acids and flavonoids. However, the extent to which these molecules contribute towards the antioxidant capacity in vitro is inconsistently reported, especially with the different analytical methods used, as well as other extrinsic factors that influence these molecules’ availability. Therefore, by reviewing recently published works correlating the vitamin, total phenolic, and flavonoid content in honey with its antioxidant activities in vitro, this paper will establish a relationship between these parameters. Based on the literature, vitamins do not contribute to honey’s antioxidant capacity; however, the content of phenolic acids and flavonoids has an impact on honey’s antioxidant activity.

scholarly journals Aktivitas Antioksidan, Kandungan Total Fenolik dan Kandungan Total Flavonoid Pada Beberapa Tumbuhan Berkhasiat Obat Di Kalimantan Timur, Indonesia

2021 ◽  
Vol 5 (2) ◽  
pp. 145
Author(s):  
Nur Maulida Sari ◽  
Irawan Wijaya Kusuma ◽  
Nur Indriana Fitriah
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Natural Antioxidants ◽  
Total Flavonoid ◽  
Assay Method ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Ceiba Pentandra ◽  
Plant Samples

Antioxidant activity, total phenolic and total flavonoid contents of some traditional medicinal plants grown in East Kalimantan, Indonesia, were investigated. Plant sample are Macaranga gigantea leaf, Ceiba pentandra leaf, Ceiba pentandra bark, Blumea balsamifera leaf and Artocarpus altilis leaf. Natural antioxidants from plant species are considered safe. Therefore, people are now more interest in finding out the treatment through natural remedies. The plant's total phenolic content was determined by the Folin-Ciocalteu method, while total flavonoid content was determined by the Colorimetric assay method. Antioxidant activity was evaluated by DPPH radical scavenging assay. The results showed that plant samples tested contained 22.02 to 214.88 µg of gallic acid equivalents (GAE)/mg extract represented the total phenolic content. The flavonoid content of the samples was in range from 50.00 to 896.67 µg of catechin equivalents (CE)/mg extract. The plant samples displayed ability to inhibit DPPH free radical formation by 59-81% at 50 ppm concentration. These finding suggest that these plants could possess natural antioxidants and give a scientific basis to the traditional uses of the investigated plants.

scholarly journals Identification and Comparative Study of Invitro Antioxidant Potential of Fractionated Hydroalcoholic Extract of Phyllanthus Niruri Linn

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Gayathri Rajamanickam ◽  
Manju S. L.
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Neurodegenerative Diseases ◽  
High Performance ◽  
Defense Mechanism ◽  
Antioxidant Activities ◽  
Natural Antioxidant ◽  
Total Phenolic ◽  
Activity Measurement ◽  
Phyllanthus Niruri

It is apparent that varieties of plants have the potential to show numerous preventive and therapeutic activities that may be relevant to the treatment of disorders caused by oxidative stress and free radicals. A variety of herbal extracts and their phyto components have been demonstrated to exert antioxidant activities, either by directly stimulating antioxidant response genes or by potentiating the bodies` own natural antioxidant defense mechanism. This study reported the high-performance liquid chromatography, invitro antioxidant activity, measurement of total phenolic and flavonoid contents of various solvent extracts of whole plant of Phyllanthus niruri in order to find possible treatment of disorders caused by oxidative stress such as neurodegenerative diseases. Results obtained revealed that, ethyl acetate fractions of P. niruri whole plants possess high flavonoid and polyphenolic compounds which ultimately leads to the potent antioxidant activity than other polar and nonpolar solvent fractions. Thus, this study suggests that, these fractions can be used as a potent source of natural antioxidant there by it can be used for the further investigation for the treatment of neurodegenerative diseases and other free radical induced pathological conditions.

scholarly journals Phenolic Profile and Antioxidant Activity of Italian Monovarietal Extra Virgin Olive Oils

Antioxidants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 161 ◽  
Author(s):  
Carmine Negro ◽  
Alessio Aprile ◽  
Andrea Luvisi ◽  
Francesca Nicolì ◽  
Eliana Nutricati ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Profile ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Ic50 Values ◽  
Phenolic Profiles ◽  
Trolox Equivalent ◽  
Phenolic Substances

In the last years, the interest in Italian monovarietal oils has increased due to their specific organoleptic qualities. Extra virgin olive oils (EVOOs) are rich in phenolic compounds, secondary metabolites well known and studied for their nutraceutical properties. However, among EVOOs, there is great variability in phenolic composition due to the origin, the production technique, and mainly, the genotype. The aim of this work was to evaluate the different phenolic profiles and the antioxidant activities of monovarietal oils. The results confirm this variability. In fact, the overall content of oleuropein varies up to four times between the different genotypes (from 33.80 to 152.32 mg/kg oil), while the oleocanthal content is significant only in two oils. The antioxidant activity, determined with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) assays, is correlated with the content of total phenolic substances, with half maximal inhibitory concentration (IC50) values for the DPPH test ranging from 160 to 91 mg of oil, while the ORAC test shows values between 5.45 and 8.03 μmol Trolox equivalent (TE)/g oil.

scholarly journals Untargeted Metabolomics of Korean Fermented Brown Rice Using UHPLC Q-TOF MS/MS Reveal an Abundance of Potential Dietary Antioxidative and Stress-Reducing Compounds

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 626
Author(s):  
Akanksha Tyagi ◽  
Su-Jung Yeon ◽  
Eric Banan-Mwine Daliri ◽  
Xiuqin Chen ◽  
Ramachandran Chelliah ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Brown Rice ◽  
Dry Weight ◽  
Untargeted Metabolomics ◽  
Total Phenolic ◽  
Germinated Brown Rice ◽  
Antioxidant Power ◽  
Flight Mass Spectrometry

Free radical-induced oxidative stress is the root cause of many diseases, such as diabetes, stress and cardiovascular diseases. The objective of this research was to screen GABA levels, antioxidant activities and bioactive compounds in brown rice. In this study, we first fermented brown rice with different lactic acid bacteria (LABs), and the best LAB was selected based on the levels of GABA in the fermentate. Lactobacillus reuterii generated the highest levels of GABA after fermentation. To ascertain whether germination can improve the GABA levels of brown rice, we compared the levels of GABA in raw brown rice (Raw), germinated brown rice (Germ), fermented brown rice (Ferm) and fermented-germinated brown rice (G+F) to identify the best approach. Then, antioxidant activities were investigated for Raw BR, Germ BR, Ferm BR and G+F BR. Antioxidant activity was calculated using a 2,2-diphenyl-1-picryl hydrazile radical assay, 2,2-azino-bis-(3-ethylene benzothiozoline-6-sulfonic acid) radical assay and ferric-reducing antioxidant power. In Ferm BR, DPPH (114.40 ± 0.66), ABTS (130.52 ± 0.97) and FRAP (111.16 ± 1.83) mg Trolox equivalent 100 g, dry weight (DW), were observed as the highest among all samples. Total phenolic content (97.13 ± 0.59) and total flavonoids contents (79.62 ± 1.33) mg GAE/100 g and catechin equivalent/100 g, DW, were also found to be highest in fermented BR. Furthermore, an untargeted metabolomics approach using ultra-high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry revealed the abundance of bioactive compounds in fermented BR, such as GABA, tryptophan, coumaric acid, L-ascorbic acid, linoleic acid, β-carotenol, eugenol, 6-gingerol, etc., as well as bioactive peptides which could contribute to the health-promoting properties of L. reuterii fermented brown rice.

scholarly journals Effect of extraction temperature and time on the extraction of phenolic compounds and antioxidant capacity of Phaleria macrocarpa fruit

2018 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Peng Yee Lim ◽  
Sook Fun Pang ◽  
Mashitah Mohd Yusoff ◽  
Jolius Gimbun
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Pearson Correlation Coefficient ◽  
Flavonoid Content ◽  
Phaleria Macrocarpa ◽  
Optimum Extraction

Phaleria macrocarpa (ver. name: ‘mahkota dewa’) is a plant which has many medically useful antioxidant activities (Anggraini & Lewandowsky, 2015). The polyphenols responsible for this antioxidant activity has to be extracted before it can be routinely used (Shwter et al., 2016). This study investigates the extraction of polyphenols from P. macrocarpa fruits and its antioxidant activity (DPPH-RSA) under influence of extraction time and temperature. By employing maceration technique, the P. macrocarpa fruits extract showed the maximum total phenolic content (TPC), total flavonoid content (TFC) and DPPH-RSA with value of 69.5 mg QE/g DW, 183.2 mg GA/g DW and 171. 8 mg BHA/g DW, respectively at  optimum extraction conditions of 60 min and 80 ºC (Fig. 1). Excellent and positive Pearson correlation coefficient with R2 > 0.91 between the TPC, TFC and antioxidant activities was observed.

scholarly journals DETERMINING SECONDARY METABOLITES AND THEIR ANTI-OXIDANT ACTIVITY IN FRUITS

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Snježana Uletilović
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Sour Cherry ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Total Phenols ◽  
Extraction Techniques ◽  
Flavonoid Content ◽  
Black Currant ◽  
Anti Oxidant

The influence of extraction techniques with different solvents on the contents of total phenols, flavonoids and antioxidant activity of extracts of grape, sour cherry and black currant is analyzed in the study. Antioxidant activity of the extracts on stable 1,1-diphenyl-2-picryl hidrazyl (DPPH) radical was determined by spectrophotometry. The total phenolic content was determined by using the Folin-Ciocalteu assay while total flavonoid content was measured spectrophotometrically with the application of AlCl3. The changes in the value of these parameters after deep-freezing of these fruits were also observed. A high content of total phenols and flavonoids was detected in extracts. Acetone proved to be the best agent for extraction of grape and sour cherry while the highest content of total phenols with black currant was obtained by methanol. The content of total phenols in fruits in frozen state increased while the content of total flavonoids and of antioxidant activity decreased. The obtained results show that the extracts of grape, cherry and black currant can be used as natural antioxidants.

scholarly journals Phenolic profile and antioxidant activity of bee pollen extracts from different regions of Algeria

2020 ◽  
Vol 31 (2) ◽  
pp. 93-98
Author(s):  
Hadia Hemmami ◽  
Bachir Ben Seghir ◽  
Mustapha Ben Ali ◽  
Abdelkrim Rebiai ◽  
Soumeia Zeghoud ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
High Performance ◽  
Pollen Grains ◽  
Biochemical Properties ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phenolic Profile ◽  
Bee Pollen ◽  
Full Diet

AbstractDue to its complex biochemical properties, the bee pollen is considered one of the functional foods. Bee pollen collected from pollen grains from different botanical sources offers almost a full diet such as carbohydrates, proteins, amino acids, vitamins, minerals. In this study, methanol extracts of 13 honeybee pollen samples were evaluated for flavonoids, phenolic compounds, and antioxidant capacity. Caffeic acid, chlorogenic acid, vanillic acid, p-coumaric acid, gallic acid, quercetin, rutin, vanillin, and naringin were identified as main phenolic compounds in pollen extracts by High-Performance Liquid Chromatography (HPLC) analysis. The obtained results are: total phenolic content - 379.8 to 915.6 mg GAE/100 g, total flavonoid content - 207.1 to 550 mg QE/100 g, and antioxidant activity - 808.2 to 3311 mg GAE/100 g in bee pollen extracts.

Phytochemical Screening, Antioxidant Activity and Total Phenolic Content of Spirogyra spp.

2013 ◽  
Vol 699 ◽  
pp. 693-697 ◽  
Author(s):  
Rattanabhorn Junthip ◽  
Doungporn Amornlerdpison ◽  
Thitiphan Chimsook
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Rapid Test ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Abts Assay

A freshwater macroalga, Spirogyra spp., were analyzed for its phytochemical composition, antioxidant activity and total phenolic content (Folin−Ciocalteu method). Phytochemical analysis of Spirogyra neglecta revealed presence of phenolics, tannins, glycosides and saponins. The crude extraction of Spirogyra spp. was carried out using two solvents via, methanol (methanolic extract) and water (aqueous extract). The total phenolic contents of crude extracts were shown at 346.58±1.61 and 589.77±1.65 mg gallic acid equivalents/g extract in aqueous and methanolic extracts, respectively. The antioxidant capacity of aqueous and methanolic extract was estimated by two different methods; ABTS assay, and DPPH assay. The antioxidant activity of two extracts is affected by the extracting solvent and different assay. In the DPPH scavenging assay and ABTS assay, both extracts showed high antioxidant activity. In addition, the high correlation between results of all antioxidant activities and total phenolic content was found. The rapid TLC assay in methanolic extract is considered as the rapid test to evaluate the antioxidant activity of natural compounds. The compounds showing four bands at Rf = 0.25, 0.35, 0.42, 0.64. This study showed that Spirogyra spp. might constitute an important source of natural antioxidants.

Comparative Antioxidant Potential of Bark and Leaves of Terminalia arjuna (Roxb) Wight & Arn from Himachal Pradesh

2017 ◽  
Vol 6 (1) ◽  
pp. 27 ◽  
Author(s):  
Vikas Kumar ◽  
Kamal Dev ◽  
Anuradha Sourirajan ◽  
Prem Kumar Khosla
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Terminalia Arjuna ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

The present study aimed to compare antioxidant activities, total phenolic and flavonoid content present in leaves and bark of Terminalia arjuna and check whether there is any correlation between phenolic content and flavonoid content with antioxidant activities or not. Phytochemical screening of ethanolic extract of leaves and bark revealed the presence of phenols, flavonoids, tannins, carbohydrates, glycosides, saponin, phytosterols and phytosteroids. Total phenolic content was found to be higher in bark (272.71±3.18 mg/g gallic acid equivalents) as compared to that of leaves (95±3.11 mg/g gallic acid equivalents). Similarly, flavonoid content of ethanolic extract of bark was found to be higher (203.95±5.13 mg/g rutin equivalents) than that of leaves (87.625±4.28 mg/g rutin equivalents). DPPH activity of ethanolic extract of bark (IC50-17.41 µg/ml) was more than that of leaves (IC50-20.22µg/ml). FRAP activity of bark (IC50- 4.781 µM Fe (II) equivalents) is more than that of leaves (IC50-7.572 µM Fe (II) equivalents). Nitric oxide (NO) scavenging activity of bark (IC50-12.87 µg/ml) was higher than that of leaves (IC50-13.91 µg/ml).The present study clearly showed that there is a correlation between total phenolics, flavonoid contents and antioxidant activity of leaves and bark of T. arjuna. The phenolic compounds and flavonoids could be major contributor to antioxidant activity of T. arjuna. Keywords: Terminalia arjuna, DPPH, FRAP, NO, IC50, Antioxidants

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