scholarly journals Characterization of Bioactive Compounds in Colored Potato (Solanum Tuberosum L.) Cultivars Grown with Conventional, Organic, and Biodynamic Methods

2020 ◽  
Vol 12 (7) ◽  
pp. 2701 ◽  
Author(s):  
Nijolė Vaitkevičienė ◽  
Jurgita Kulaitienė ◽  
Elvyra Jarienė ◽  
Dovilė Levickienė ◽  
Honorata Danillčenko ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
High Performance ◽  
Solanum Tuberosum L ◽  
Principal Component ◽  
Coumaric Acid ◽  
Chromatography Method ◽  
Organic Cultivation ◽  
Liquid Chromatography Method

The purpose of this research is to evaluate the effect of conventional, biodynamic, and organic cultivation methods on the contents of polyphenols and carotenoids in tubers of potato cultivars with different colored flesh: “Red Emmalie”, “Salad Blue”, “Violetta”, “Tornado”, and “Laura”. These bioactive compounds were determined by the high-performance liquid chromatography method. The data received were analyzed with ANOVA and further explored and visualized by principal component analysis. Higher contents of polyphenols (sum), phenolic acids (sum), chlorogenic acid, p-coumaric acid, and caffeic acid were found in biodynamic and organic samples compared to the conventional tubers. Moreover, organically and biodynamically produced potatoes (except “Salad Blue” cultivar) were significantly richer in flavonoids and anthocyanins. The highest contents of carotenoids (sum), lutein, and β-carotene were found in biodynamic potatoes. Among the tested cultivars, “Tornado”, with white flesh, was richest in polyphenols (sum), phenolic acids (sum), chlorogenic acid, and p-coumaric acid. “Violetta”, with dark purple flesh, accumulated the highest contents of flavonoids (sum), anthocyanins (sum), petunidin-3,5-di-O-glucoside, pelargonidin-3,5-di-O-glucoside, and peonidin-3,5-di-O-glucoside. Carotenoids were only found in “Laura” tubers, and the dominating carotenoid was lutein.

Characterization by HPLC of p-Hydroxybenzyl Alcohol Biotransformation to Gastrodin In Vivo

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110350
Author(s):  
Lijun Cheng ◽  
Yang Deng
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Array Detector ◽  
Gastrodia Elata ◽  
Chromatography Method ◽  
Liquid Chromatography Method ◽  
First Time ◽  
Insight Into ◽  
Hydroxybenzyl Alcohol

Gastrodin (GAS) and its aglycone, p-hydroxybenzyl alcohol (HBA), are both bioactive compounds extracted from Gastrodia elata Blume (GEB). In the current Chinese pharmacopoeia, they are regarded as quality control markers for GEB. In this study, we developed a high-performance liquid chromatography method coupled with a diode array detector to quantify GAS and HBA concentrations in plasma following oral ingestion by rats. For the first time, GAS was detected in vivo after HBA administration. GAS and HBA both had similar pharmacological effects, but the influence of the glucose moiety resulted in different pharmacokinetic characteristics. In this study, the effects of GAS and HBA at different administration durations were investigated in zebrafish larvae. These compounds were found to induce a sedative effect but had different onset times. In conclusion, a biotransformation of HBA to GAS could be observed in the rats. This may be a new insight into the pharmacokinetic characteristics of these bioactive compounds and also relates to the different ways in which they take effect.

scholarly journals The Profile of Selected Antioxidants in Two Courgette Varieties from Organic and Conventional Production

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 404
Author(s):  
Klaudia Kopczyńska ◽  
Renata Kazimierczak ◽  
Dominika Średnicka-Tober ◽  
Marcin Barański ◽  
Zdzisław Wyszyński ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Phenolic Acids ◽  
High Performance ◽  
Antioxidant Properties ◽  
Good Method ◽  
Global Scale ◽  
Scientific Publications ◽  
Organic Cultivation ◽  
Growing Conditions ◽  
The Impact

Courgette is considered as a low-calorie vegetable with health-promoting properties. However, scientific publications focused on the profile and content of bioactive compounds in courgette, as well as the potential fruit quality modulating factors, are rare. Due to the high adaptability of courgette to weather and agronomic conditions, it is produced on a global scale. The aim of this study was to analyse the impact of organic versus conventional agronomic practices on the concentration of selected antioxidants in courgette fruits. Fruits of two courgette varieties (Astra Polka and Nimba) produced in an organic and conventional system were tested by high performance liquid chromatography (HPLC) to determine the content of polyphenols (flavonoids and phenolic acids), carotenoids, chlorophylls, and vitamin C. Organic courgette fruits were characterised by their significantly higher content of phenolic acids and flavonoids when compared to the conventionally grown fruit. The organic cultivation might be a good method to increase concentration of bioactive compounds with antioxidant properties in courgette fruits. Nevertheless, the identified trends should be further confirmed, with attention paid to other courgette varieties, as well as to the potential interactions between the plant genotype, agronomic system and the location-specific growing conditions.

scholarly journals Phenolic profiles and antioxidant activities of exocarp, endocarp, and hypanthium of three pear cultivars grown in China

2021 ◽  
Vol 14 ◽  
Author(s):  
Bin Du ◽  
Caihong Cheng ◽  
Yuqin Chen ◽  
Jinhui Wu ◽  
Fengmei Zhu ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Chlorogenic Acid ◽  
Acid Content ◽  
High Performance ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Chromatography Method ◽  
Pharmaceutical Application ◽  
Phenolic Profiles ◽  
Liquid Chromatography Method

The phenolic composition of the exocarp, hypanthium, and endocarp from three pear cultivars: Jingbai, Korla and Crystal were measured by high-performance liquid chromatography method with diode array detection (HPLC-DAD), while antioxidant activities were investigated using DPPH radical andABTS radical cation scavenging activity, and FRAP. Ferulic acid, chlorogenic acid, and caffeic acid were detected as major components. The exocarp of the Jingbai pear had a relatively high chlorogenic acid content (0.691 mg kg-1) while the hypanthium and endocarp had the high chlorogenic acid content of the three pear varieties. The exocarp of the Korla pear had the highest ferulic acid and rutin content. The exocarp, hypanthium, and endocarp of the Crystal pear had the highest content of chlorogenic acid of the three pear varieties. The Crystal pear presented the highest total phenolic and flavonoid contents and had the highest antioxidant activities in terms of FRAP. The Jingbai pear exocarp presented the highest ABTS value, 83.62 ± 0.08%. The endocarp of the Crystal pear had the highest DPPH value, 87.30 ± 0.03%. Positive correlations were detected among chlorogenic acid, TPC, TFC, and FRAP. The results reveal that the different parts of Pyrus communis L. have effective antioxidant activity for the pharmaceutical application.

scholarly journals Фенольні сполуки листя Sorbus domestіca і Sorbus graeca

2018 ◽  
Author(s):  
O. V. Krivoruchko
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Hplc Method ◽  
Chromatography Method ◽  
Pharmacological Studies ◽  
Rosaceae Family ◽  
Liquid Chromatography Method

Introduction. Sorbus domestіca and Sorbus graeca from the Rosaceae family are cultivated in Ukraine in gardens and parks. Their chemical composition is investigated insufficiently.The aim of the study – to research the composition and the content of phenolic compounds of leaves of Sorbus domestіca and Sorbus graeca.Research Methods. The content of phenolic compounds in leaves of Sorbus domestіca and Sorbus graeca was carried out by the high performance liquid chromatography method on Agilent Technologies chromatograph.Results and Discussion. In leaves of Sorbus domestіca chlorogenic acid, rutin, quercetin-3-O-glucoside and quercetin were identified; in leaves of Sorbus graeca chlorogenic and neochlorogenic acids, rutin and 4'-methoxyquercetin-3-O-sophoroside were identified. The content of hydroxycinnamic acids in leaves of Sorbus domestіca is 389 mg/100 g, in leaves of Sorbus graeca – 147 mg/100 g (in terms of chlorogenic acid). The content of flavonoids in leaves of Sorbus domestіca is 1888 mg/100 g, in leaves of Sorbus graeca – 727 mg/100 g (in terms of rutin).Conclusions. The composition and the content of phenolic compounds of Sorbus domestіca and Sorbus graeca were studied by the HPLC method. Leaves of Sorbus domestіca are more perspective for further pharmacological studies.

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