Comprehensive functional analysis reveals that acrosome integrity and viability are key variables distinguishing artificial insemination bulls of varying fertility

This study focused on the identification of naturally occurring bacteria in the reproductive fluid and impact on the quality of ejaculates obtained from the turkey breed British United Turkeys (BUT) Big 6 (n = 60). We determined possible relationships between the bacterial load and advanced sperm quality parameters that are important for effective artificial insemination and high fertility, as well as the concentration of selected antimicrobial proteins and pro-inflammatory markers of turkey semen. Sperm motility was assessed with computer-assisted sperm analysis (CASA), while the membrane and acrosome integrity were examined with smearing and staining methods. Reactive oxygen species (ROS) generation was quantified via luminometry, sperm DNA fragmentation was evaluated using the TUNEL assay, and the JC-1 assay was applied to evaluate the mitochondrial membrane potential. Cell lysates were prepared to investigate the extent of lipid and protein oxidation. Furthermore, levels of interleukins 1 and 6 (IL-1, IL-6), C-reactive protein, cathelicidin, and β-defensin were quantified in the seminal plasma using the ELISA method. The most dominant species identified by the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry was Escherichia coli, Proteus mirabilis, Staphylococcus lentus, and Citrobacter braakii. The bacterial load had a negative effect on the sperm motility (p < 0.001), as well as membrane (p < 0.05) and acrosome integrity (p < 0.01). A strong positive relationship between the bacterial load and DNA fragmentation (p < 0.001) was detected as well. Positive associations were recorded between the increasing presence of bacteria, ROS overgeneration (p < 0.001), and a subsequent oxidative damage to the proteins (p < 0.001) and lipids (p < 0.01). It was revealed that the antimicrobial peptides β-defensin (p < 0.001) and cathelicidin (p < 0.001) had a positive relationship with the motility. In contrast, pro-inflammatory markers, such as IL-1 (p < 0.001) and IL-6 (p < 0.001), had a negative impact on the motion behavior of turkey spermatozoa. Our results suggest that the semen quality may be notably affected by the bacterial quantity as well as quality. It seems that bacteriospermia is associated with inflammatory processes, oxidative stress, sperm structural deterioration, and a subsequent risk for a failed artificial insemination in turkey breeding.

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Efek Lama Penyimpanan Semen Beku Sapi Bali pada Pos Inseminasi Buatan terhadap Membran Plasma, Tudung Akrosom Utuh, dan DNA Spermatozoa

Jurnal Ilmu Peternakan Terapan ◽

10.25047/jipt.v3i2.1950 ◽

2020 ◽

Vol 3 (2) ◽

pp. 58-66

Author(s):

Fikri Ardhani ◽

Hayatul Mufidah ◽

Rahmah Samsuriati ◽

Hilman Pratama Putra

Keyword(s):

Dna Damage ◽

Plasma Membrane ◽

Artificial Insemination ◽

Storage Time ◽

Membrane Integrity ◽

Frozen Storage ◽

Plasma Membrane Integrity ◽

East Kalimantan ◽

Frozen Semen ◽

Acrosome Integrity

The purpose of this study was to determine the effect of frozen storage time for Bali Bull in artificial insemination station in Samarinda City, East Kalimantan on the quality of motility, viability, velocity, abnormality, plasma membrane integrity (MIn), acrosome integrity (AIn), and DNA damage of spermatozoa. The study design used a completely randomized design (CRD) with 5 treatments (storage time) and 5 replications. Frozen semen of Bali Bull used in 2009 (10 years of storage), 2011 (7 years of storage), 2013 (5 years of storage), 2015 (3 years of storage), and 2017 (1 year of storage). The storage time of frozen semen stored for one to ten years in liquid nitrogen at the artificial insemination station in Kota Samarinda, East Kalimantan was still suitable for use in artificial insemination based on motility quality (44.99±2.40%), viability (55.33±2,60%), velocity (0.050±0.002 mm/sec), abnormality (12.87±1.09%), plasma membrane integrity (58.83 ± 1.86%), acrosome integrity (75.48 ± 1 , 61%), and DNA damage of spermatozoa (1.60 ± 0.21%).

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Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program

Veterinary World ◽

10.14202/vetworld.2020.2649-2657 ◽

2020 ◽

Vol 13 (12) ◽

pp. 2649-2657

Author(s):

Berlin Pandapotan Pardede ◽

Muhammad Agil ◽

Yudi Yudi ◽

Iman Supriatna

Keyword(s):

Dna Fragmentation ◽

Artificial Insemination ◽

Semen Quality ◽

Fertility Rate ◽

Breeding Program ◽

Sperm Viability ◽

Production Site ◽

Frozen Semen ◽

Acrosome Integrity

Background and Aim: Various factors can reduce the quality of semen used for artificial insemination and have an impact on fertility decline, such as poor handling during frozen semen distribution. This study was aimed at assessing the quality of frozen-thawed semen after distribution in the field and its importance in maintaining fertility. Materials and Methods: The Brahman Cross (BX) breeding program of PT Lembu Jantan Perkasa, Indonesia, was used. This program was preferred due to its adherence to guidelines that limit the effects of extraneous factors that may affect semen quality. Frozen-thawed semen samples from eight bulls with the same production code were analyzed and compared between the production site (artificial insemination [AI] center) and the field (BX breeding program). Total and progressive motility (PM) of sperm were determined using computer-assisted semen analysis. Plasma membrane integrity (PMI) was assessed using hypoosmotic swelling test, sperm viability using Eosin-Nigrosin staining, acrosome integrity using trypan blue-Giemsa staining, morphological abnormalities using William staining, and DNA fragmentation using toluidine blue staining. The fertility rate was determined using the conception rate (%) derived from AI data based on 502 AI services and 478 cows in the BX breeding program. A t-test was used to compare the quality of frozen-thawed semen before and after distribution. The relationship between the qualities of frozen semen after distribution in the field with fertility was analyzed using Pearson correlation. Results: There was no significant difference (p>0.05) in the quality of frozen-thawed semen (sperm motility, PMI, viability, acrosome integrity, abnormalities, and DNA fragmentation) between the production site (AI center) and after distribution in the field (BX breeding program). The semen met the minimum standards for AI programs. Total motility (r=0.986), PM (r=0.961), sperm viability (r=0.971), PMI (r=0.986), and acrosome integrity (r=0.992) were all positively correlated (p<0.05) with fertility rate; while sperm abnormalities (r=-0.996) and sperm DNA fragmentation (r=0.975) were negatively correlated (p<0.05) with fertility rate. Conclusion: The study showed that to achieve the maximal and optimal fertility rate in bulls in an AI program, the overall quality of frozen-thawed semen in all aspects is critical. This can be achieved if the handling during distribution and storage, as well as the various factors that may affect the quality of semen in the field, can be controlled properly.

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Viability of caprine spermatozoa in sodium citrate-egg yolk extender at room temperature

Nigerian Journal of Animal Production ◽

10.51791/njap.v45i3.404 ◽

2020 ◽

Vol 45 (3) ◽

Author(s):

E. Ewuola ◽

U.D.G. Ihejirika ◽

T.F. Adepoju

Keyword(s):

Artificial Insemination ◽

Room Temperature ◽

Sodium Citrate ◽

Egg Yolk ◽

Negative Control ◽

West African ◽

Dilution Ratio ◽

Spermatozoa Motility ◽

Acrosome Integrity ◽

On Farm

This work was aimed at evaluating the ability of sodium citrate-egg yolk extender to sustain the viability of spermatozoa in semen stored at room temperature over a period of time as well as determining the best dilution ratio for on-farm artificial insemination. Seven matured West African dwarf bucks were used for this study; semen was collected once weekly with electro ejaculator, maintained at 37 C in the water bath during extension and was evaluated immediately after extension at room temperature until motility dropped below 20%. The semen collected were pooled and divided into 5 portions. Each portion was extended with sodium citrate-egg yolk extender at 1:0 (without extender, control) as T1, (1:1) T2, (1:2) T3, (1:3) T4 and the 5th portion was extended with normal saline at 1:1 (T5) which served as the negative control. Sperm motility, spermatozoa concentration, livability, acrosome integrity and morphological abnormalities were assessed and data obtained were subjected to analysis of variance. Results showed that spermatozoa motility in treatments 1, 2, and 5 differed significantly (P

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Clinical Issues: What’s in a Word? A Functional Analysis of Word Learning

Perspectives on Language Learning and Education ◽

10.1044/lle12.3.4 ◽

2005 ◽

Vol 12 (3) ◽

pp. 4-8 ◽

Cited By ~ 7

Author(s):

Prahlad Gupta

Keyword(s):

Functional Analysis ◽

Word Learning ◽

Clinical Issues

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Review for "High‐throughput single‐cell sequencing of paired TCRα and TCRβ genes for the direct expression‐cloning and functional analysis of murine T cell receptors"

10.1002/eji.201848030/v1/review1 ◽

2019 ◽

Keyword(s):

Functional Analysis ◽

T Cell ◽

Single Cell ◽

High Throughput ◽

T Cell Receptors ◽

Expression Cloning ◽

Cell Receptors ◽

Single Cell Sequencing

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Referral Patterns for Youths Identified at Risk for Suicide by Trained Gatekeepers

Crisis ◽

10.1027/0227-5910/a000114 ◽

2012 ◽

Vol 33 (2) ◽

pp. 113-119 ◽

Cited By ~ 10

Author(s):

Michael S. Rodi ◽

Lucas Godoy Garraza ◽

Christine Walrath ◽

Robert L. Stephens ◽

D. Susanne Condron ◽

...

Keyword(s):

Suicide Prevention ◽

Demographic Characteristics ◽

Service Access ◽

Referral Patterns ◽

Race And Gender ◽

Lee Smith ◽

Prevention Behavior ◽

Key Variables ◽

And Gender ◽

To Receive

Background: In order to better understand the posttraining suicide prevention behavior of gatekeeper trainees, the present article examines the referral and service receipt patterns among gatekeeper-identified youths. Methods: Data for this study were drawn from 26 Garrett Lee Smith grantees funded between October 2005 and October 2009 who submitted data about the number, characteristics, and service access of identified youths. Results: The demographic characteristics of identified youths are not related to referral type or receipt. Furthermore, referral setting does not seem to be predictive of the type of referral. Demographic as well as other (nonrisk) characteristics of the youths are not key variables in determining identification or service receipt. Limitations: These data are not necessarily representative of all youths identified by gatekeepers represented in the dataset. The prevalence of risk among all members of the communities from which these data are drawn is unknown. Furthermore, these data likely disproportionately represent gatekeepers associated with systems that effectively track gatekeepers and youths. Conclusions: Gatekeepers appear to be identifying youth across settings, and those youths are being referred for services without regard for race and gender or the settings in which they are identified. Furthermore, youths that may be at highest risk may be more likely to receive those services.

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Case Formulation and Design of Behavioral Treatment Programs

European Journal of Psychological Assessment ◽

10.1027//1015-5759.19.3.164 ◽

2003 ◽

Vol 19 (3) ◽

pp. 164-174 ◽

Cited By ~ 27

Author(s):

Stephen N. Haynes ◽

Andrew E. Williams

Keyword(s):

Functional Analysis ◽

Behavior Problems ◽

Behavioral Treatment ◽

Clinical Case ◽

Relative Magnitude ◽

Mechanisms Of Action ◽

Treatment Programs ◽

Case Formulation ◽

Functional Relations ◽

Causal Variables

Summary: We review the rationale for behavioral clinical case formulations and emphasize the role of the functional analysis in the design of individualized treatments. Standardized treatments may not be optimally effective for clients who have multiple behavior problems. These problems can affect each other in complex ways and each behavior problem can be influenced by multiple, interacting causal variables. The mechanisms of action of standardized treatments may not always address the most important causal variables for a client's behavior problems. The functional analysis integrates judgments about the client's behavior problems, important causal variables, and functional relations among variables. The functional analysis aids treatment decisions by helping the clinician estimate the relative magnitude of effect of each causal variable on the client's behavior problems, so that the most effective treatments can be selected. The parameters of, and issues associated with, a functional analysis and Functional Analytic Clinical Case Models (FACCM) are illustrated with a clinical case. The task of selecting the best treatment for a client is complicated because treatments differ in their level of specificity and have unequally weighted mechanisms of action. Further, a treatment's mechanism of action is often unknown.

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