Recovery of Residual Organic Matter from In Vitro Digestion of Forages

Twenty experimental silages were made from seven pasture species at different stages of maturity. In vivo dry matter, organic matter, and energy digestibilities of the silages were determined at restricted and ad libitum intake levels on standardized pairs of Merino wethers. In vitro dry matter digestibility determinations were made on ground frozen silage and ground oven-dried silage with and without a correction for the loss of volatile compounds during drying. Corrected in vitro dry matter digestibilities of ground oven-dried silage for a 48 hr rumen liquor digestion time gave the most accurate prediction of in vivo dry matter (r = 0.88), organic matter (r = 0.86), and energy (r = 0.74) digestibilities for restricted intake levels. In vitro digestibilities of ground frozen silage more accurately predicted in vivo digestibilities than did in vitro digestibilities of ground oven-dried silage without the correction for volatile losses. In vivo dry matter and organic matter digestibilities were highly correlated (v = 0.97), but both were poorly correlated with in vivo energy digestibilities. In vitro digestion of ground oven-dried silage with a correction for the volatile compounds lost during drying is recommended as a suitable routine determination for predicting the in vivo digestibility of silage.

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IN VITRO DIGESTION OF WHEAT STRAW BY DIFFERENT RUMEN ANAEROBIC FUNGI

Canadian Journal of Animal Science ◽

10.4141/cjas84-202 ◽

1984 ◽

Vol 64 (5) ◽

pp. 156-157 ◽

Cited By ~ 17

Author(s):

G. L. R. GORDON ◽

J. R. ASHES

Keyword(s):

Organic Matter ◽

Key Words ◽

Wheat Straw ◽

In Vitro Digestion ◽

Neutral Detergent Fiber ◽

Anaerobic Fungi ◽

Acid Detergent Fiber ◽

Fiber Digestion

Two types of anaerobic fungi were isolated from the rumen of a sheep fed wheat straw. The fungus producing a mycelium in culture digested significantly more organic matter, neutral detergent fiber, acid detergent fiber and cellulose from straw than the type producing a sporangium from "spherical bodies." Key words: Rumen, fungi, straw, fiber digestion

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Note on in vitro digestion of avocado products for pigs

South African Journal of Animal Science ◽

10.4314/sajas.v51i1.16 ◽

2021 ◽

Vol 51 (1) ◽

Author(s):

J. Ly ◽

P. Fránquez ◽

G. Rodríguez ◽

C. Lemus ◽

I.A. Dominguez ◽

...

Keyword(s):

Organic Matter ◽

Dry Matter ◽

Nutritional Value ◽

In Vitro Digestion ◽

In Vitro Digestibility ◽

Optimal Production ◽

Additional Protein

In vitro (pepsin/pancreatin) digestibility coefficients of pulp, seed, and peel of avocados of the Creole and Hass varieties were studied according to a 2 x 3 factorial arrangement in which the factors were the variety and parts of the fruit. Creole avocado were heavier in natura than Hass exemplars (310.7 g and 156.8 g), whereas Hass had a greater proportion of pulp than the Creole fruits (76.0% and 64.6%). No significant interactions were observed. In vitro digestibility of dry matter (DM), organic matter (OM), and nitrogen (N) were higher for the Creole variety compared with the Hass. In vitro digestibility of N was similar in the pulp and seeds, and higher than in the peels (731, 773, and 550 g/kg-1, respectively). Use of the entire avocado as pig feedstuff would have a lower nutritional value compared with feeding just avocado pulp. Supplementation of a diet based on avocado with additional protein may be needed for optimal production.

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INTERACTION BETWEEN INOCULUM DONOR DIET AND SUBSTRATE IN IN VITRO RUMINANT DIGESTION STUDIES

Canadian Journal of Animal Science ◽

10.4141/cjas66-014 ◽

1966 ◽

Vol 46 (2) ◽

pp. 91-95 ◽

Cited By ~ 7

Author(s):

J. E. Knipfel ◽

J. E. Troelsen

Keyword(s):

Organic Matter ◽

Dry Matter ◽

In Vitro Digestion ◽

Barley Grain ◽

Absolute Amount ◽

In Vitro Digestibility ◽

Promoting Effect ◽

Digestion Technique ◽

The Difference

Six wethers with rumen fistula were each fed a different combination of alfalfa hay, wheat straw, and barley grain. The same mixtures were fermented for periods of 12 and 48 hours by inoculum from each wether, using a two-stage in vitro digestion technique. The in vitro digestibility of dry matter and organic matter was determined on each treatment combination.The digestibility data revealed interactions among inoculum donor diets, in vitro substrate mixtures, and fermentation periods. When the diet lacked one or more of the substrate components, a 12-hour artificial rumen fermentation resulted in digestibilities that decreased as the composition of the substrate deviated increasingly from that of the donor diet. This effect was eliminated by extending the fermentation period to 48 hours. When all the substrate ingredients were included in the donor diet, the absolute amount of each ingredient, within 10 to 80%, was of less importance.Indications were that alfalfa possessed a specific growth-promoting effect on the microflora.Increasing the proportion of alfalfa in the substrate increased the difference between the in vitro digestibility of dry matter and organic matter. This was suggested to result from a higher concentration of soluble ash in the alfalfa fraction of the mixtures.

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In Vitro Digesting Measurement of Cassava Leaves Using Gizzard Fluid and Chicken Duodenum

Bantara Journal of Animal Science ◽

10.32585/bjas.v3i2.1947 ◽

2021 ◽

Vol 3 (2) ◽

pp. 58

Author(s):

Ali Mursyid Wahyu Mulyono ◽

Engkus Ainul Yakin ◽

Muhammad Affan Azizy Hasibuan

Keyword(s):

Organic Matter ◽

Protein Content ◽

Organic Material ◽

In Vitro Digestion ◽

Feed Material ◽

Alternative Feed ◽

Cassava Leaves ◽

Randomized Design

Cassava leaves are an alternative feed material that can be a feed material with sufficient protein content. The study aimed to determine the effect of gizzard and duodenal fluids on in vitro digesting measurements of cassava leaves. The study used a Complete Randomized Design (RAL) unidirectional pattern, Variance Analysis (ANOVA) with Duncan's Multiple Range Terst (DMRT) follow-up test using the SPSS application. The study used 4 treatments and 3 repeats: P0: No digestive fluids (controls), P1: Gizzard fluid, P2: Duodenal fluid, P3: A mixture of gizzard and duodenal fluids. The observed variables are the Dry Material Digest Coefficient (KCBK), the Organic Material Digest Coefficient (KCBO), and the Dissolved Protein Digest Coefficient (KCPT). The results of the study that the use of gizzard fluid in in vitro digestion measurements was better in increasing the value of in vitro digestant of cassava leaves. The use of gizzard fluid can increase the digestibility coefficient of organic matter (KCBO) by 21.2160% and the dissolved protein digest coefficient (KCPT) by 50.4620%.

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SUPLEMENTASI BAGASE DENGAN ENZIM SELULASE DAN PENGARUHNYA TERHADAP KECERNAAN BAHAN KERING, BAHAN ORGANIK DAN SERAT KASAR SECARA IN VITRO

Jurnal Riset Kimia ◽

10.25077/jrk.v1i1.98 ◽

2015 ◽

Vol 1 (1) ◽

pp. 84

Author(s):

Yetti Marlida ◽

Mardiati Zain

Keyword(s):

Organic Matter ◽

Dry Matter ◽

Animal Feed ◽

In Vitro Digestion ◽

Industrial Biotechnology ◽

Liquid Form ◽

Industrial Residues ◽

Cellulase Enzyme ◽

Key Words Cellulase

ABSTRACTSupplementation of cellulase enzyme to agro-industrial residues can increase the nutritional value of the product. Advances in industrial biotechnology offer potential opportunities for economic utilization of agro-industrial residues such as palm pres fiber, rice straw, corn straw and bagase for animal feed. Bagase is a complex material, is the major by product of the sugar cane industry. This experiment examined the effect of applying a cellulase enzyme (Siqma & Co) on the digestibility of bagase in vitro. The enzyme was applied in liquid form at concentrations 1.0; 1.5 and 2.0 (g/100 g DM) to bagase. The bagase was incubated with enzyme for 48 h before in vitro digestion. The digestibility of dry matter, organic matter and fiber in vitro was detected. The results showed that digestibility of dry matter, organic matter and fiber were significantly different (P<0.01) across treatments. The increased of the cellulase enzyme supplemented to bagase can increased the nutritive values of the product and directly digestibility of dry matter, organic matter and fiber higher. Results from this study indicated that direct application of enzymes to bagase was capable to improving digestibility in-vitro. Key words : cellulase enzyme, bagase, digestibility, in vitro

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