scholarly journals In Vitro Digesting Measurement of Cassava Leaves Using Gizzard Fluid and Chicken Duodenum

2021 ◽  
Vol 3 (2) ◽  
pp. 58
Author(s):  
Ali Mursyid Wahyu Mulyono ◽  
Engkus Ainul Yakin ◽  
Muhammad Affan Azizy Hasibuan

Cassava leaves are an alternative feed material that can be a feed material with sufficient protein content. The study aimed to determine the effect of gizzard and duodenal fluids on in vitro digesting measurements of cassava leaves. The study used a Complete Randomized Design (RAL) unidirectional pattern, Variance Analysis (ANOVA) with Duncan's Multiple Range Terst (DMRT) follow-up test using the SPSS application. The study used 4 treatments and 3 repeats: P0: No digestive fluids (controls), P1: Gizzard fluid, P2: Duodenal fluid, P3: A mixture of gizzard and duodenal fluids. The observed variables are the Dry Material Digest Coefficient (KCBK), the Organic Material Digest Coefficient (KCBO), and the Dissolved Protein Digest Coefficient (KCPT). The results of the study that the use of gizzard fluid in in vitro digestion measurements was better in increasing the value of in vitro digestant of cassava leaves. The use of gizzard fluid can increase the digestibility coefficient of organic matter (KCBO) by 21.2160% and the dissolved protein digest coefficient (KCPT) by 50.4620%.

2006 ◽  
Vol 12 (2) ◽  
pp. 193
Author(s):  
Jesús Fuentes ◽  
Antonio Cruz ◽  
Lorenzo Castro ◽  
Gilberto Gloria ◽  
Sergio Rodríguez ◽  
...  

This research was conducted to evaluate 16 corn varieties cultivated for silage. The evaluation included: fodder production of green and dry matter, protein content and in vitro dry matter digestibility (IVDMD) and organic matter digestibility (IVOMD). A completely randomized design was used for statistical analysis and Tukey mean separation was used when appropiated. Fodder production as kg/ha was higher for AN446 (114028), AN445 (110993), and AN430R (106389). Dry matter fodder production was higher for AN447 (29270), AN461 (28026), VS373 (25434). Protein content (%) was higher for G4657 (7.58), AN388 (7.28), and AN448 (7.17). The best values for IVDMD (%)were for G1990 (69.14), AN446 (63.78), and AN430RR (63.50), while the higher values for IVOMD (%) were for G1990 (71.78), AN446 (65.20), and AN447 (65.12). Varieties AN446 and AN447 appeared frequently with the best values in the parameters evaluated, therefore, new research has to be performed with these varieties to confirm results found in this study.


2015 ◽  
Vol 2 (3) ◽  
pp. 17
Author(s):  
Indrayani Indrayani ◽  
Harapin Hafid ◽  
Dian Agustina

ABSTRACTThis study aims to determine the digestibility of dry matter and organic matter level waste silage mixed vegetables and Gliricidia leaves were tested in vitro. This study used a completely randomized design with 3 treatments and 3 replications. Each of these treatments is R0 (Gliricidia leaves 100%), R1 (Gliricidia leaves 70% + 30% silage vegetable waste), and R2 (Gliricidia leaves 40% + 60% silage vegetable waste). Data were analyzed using analysis of variance (ANOVA) and further testing using the test Honestly Significant Difference (HSD). The results of ANOVA showed that the mixture of vegetable waste silage was highly significant (p <0.05) on dry matter digestibility and significantly (p <0.05) on the digestibility of organic matter. It can be concluded that the mixed of vegetable waste silage and Gliricidia leaves can improved digestibility of dry matter and organic matter, treatment of 40% and 60% Gliricidia leaves plus waste vegetable produce silage dry matter digestibility and percentage of organic matter is best (72,24% and 68,19%).Keyword: Silage vegetable waste, gliricidia leaves, dry and organic matter digestibilityABSTRAKPenelitian ini bertujuan untuk mengetahui kecenaan bahan kering dan bahan organik tingkat campuran silase sampah sayur dan daun gamal yang diuji secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 3 ulangan. Masing-masing perlakuan tersebut ialah R0 (daun gamal 100%), R1 (daun gamal 70% + silase sampah sayur 30%), dan R2 (daun gamal 40% + silase sampah sayur 60%). Data yang diperoleh dianalisis dengan menggunakan analisis sidik ragam (ANOVA) dan uji lanjut menggunakan uji Beda Nyata Jujur (BNJ). Hasil analisis sidik ragam menunjukkan bahwa campuran silase sampah sayur berpengaruh sangat nyata (p<0,05) terhadap kecernaan bahan kering dan berpengaruh nyata (p<0,05) terhadap kecernaan bahan organik. Dapat disimpulkan bahwa perlakuan campuran silase sampah sayur dan daun gamal dapat meningkatkan kecernaan bahan kering dan bahan organik, perlakuan 40% daun gamal dan 60% silase sampah sayur menghasilkan persentase kecernaan bahan kering dan bahan organik yang terbaik yaitu (72,24% dan 68,19%).Kata kunci : Silase sampah sayur, daun gamal, kecernaan bahan kering, dan bahan organik.


1970 ◽  
Vol 17 (1) ◽  
Author(s):  
Ari L. Darmawan ◽  
ASEP IRAWAN ◽  
TIDI DHALIKA ◽  
ANA R. TARMIDI ◽  
MANSYUR ◽  
...  

Palm oil fiber has its potency as feed fiber source for ruminant, but contains high lignin and causes limited digestibility. This research was carried out to find the effect of soaking palm oil fiber in filtrated palm oil fruit bunch ash (FPOFBA) on in vitro digestibility. This experiment used a completely randomized design that repeated for 4times. Palm oil fruit bunch ash was mixed in water and entered into container for 24 hours within concentrations, consists of: 50, 100, 150 and 200 g/L. Moreover, this filtrate used to soak palm oil fiber for 3 hours. The processed products were analyzed for their level of lignin and crude fiber. Meanwhile, in vitro test was used to measure digestibility. It showed that soaking in filtrated palm oil fruit bunch ash gave significant effect to decrease level of lignin and crude fiber (P<0.05), and without soaking to improve digestibility of dry and organic matter compared to control treatment (P<0.05). Treatment with 150 g/L and 200 g/L using filtrated palm oil fruit bunch ash produced low level of lignin (P<0.05), as of: 17.25 and 18.53%. In contrast, within concentration of 150 g/L produced higher level of crude fiber compared to 200 g/L (46.04 vs. 43.87%). This seemed to have the same results in digestibility of dry and organic matter (P>0.05), such as 23.48 and 24.12% as well as 16.70 and 17.06 % in each. It can be concluded that soaked palm oil fiber with 150 g/L concentration of filtrated palm oil fruit bunch ash was more effective in improving digestibility.


2015 ◽  
Vol 39 (2) ◽  
pp. 71
Author(s):  
Iman Hernaman ◽  
Atun Budiman ◽  
Siti Nurachman ◽  
Kundrat Hidrajat

<p>This research was aimed to investigate the effect of concentrate substitution with cassava plantation waste supplemented with cobalt and zinc in sheep ration. In vitro study was used in this experiment. Collected data were analyzed by Duncan’s test from Completely Randomized Design with four treatments and four replications. The experiment rations were R1 = 50% native grass + 50% concentrate, R2 = 50% native grass + 50% concentrate + 5 ppm cobalt + 30 ppm zinc, R3 = 50% native grass + 50% cassava plantation waste, R4 = 50% native grass + 50% cassava plantation waste + 5 ppm cobalt + 30 ppm zinc. The results showed that utilization of concentrate increased volatile fatty acid and N-NH3 concentration, and digestibility of dry and organic matter compared with cassava plantation waste. Volatile fatty acid and N-NH3 in sheep ration containing cassava plantation waste were still in normal range with dry and organic matter digestibility were up to 50%, but supplementation of cobalt and zinc had the same effect (P&gt;0.05). It is concluded that cassava plantation waste can be used as sheep feed, but did not substitute concentrate. Supplementation of cobalt and zinc was not effective to improve fermentability and digestibility of cassava plantation waste.</p><p><br />(Key words: Cassava plantation waste, Digestibility, In vitro, N-NH3, Sheep, Volatile fatty acid)</p>


2015 ◽  
Vol 2 (2) ◽  
pp. 124-131
Author(s):  
Khusrizal

The Inceptisols is a soil in great amount and widely distributed in Indonesia, even in the province of Aceh Inceptisols is the most extensive amount. The Inceptisols were found from lowland up to highland, and their properties are very varied. Commonly, the soil fertility of the Inceptisols of lowland is low. Calcite and Organic Matter are the easy matter found, and to be considered as a better soil amendment. While maize is improved varieties of crops that its production constantly to be increased. This experiment was conducted in order to study the contribution of organic matter as well as calcite contribution to the change of iron and manganese in soil and uptake by maize at North Aceh Inceptisol. Kinds of organic matter that was used consist of cow manure and water hyacinth (60:40). The fully randomized design has been conducted that consists of two combined treatment factors with three replicated. The factor is related to the kinds of organic matter was 0.0, 2.0, 3.0 and 4.0 ton ha-1 and calcite was 0.0, 1.0 and 1.5 ton ha-1. The results showed that the application of organic matter and calcite significantly affect levels of iron and manganese in the soil and plants. Dose organic material 4 ton ha-1 and calcite 1.5 tons ha-1 are able to increase at the highest level of the iron content of the soil, while the dose of organic materials 4 ton ha-1 and calcite dose 0.0-1.0 tonnes ha-1 is the best for the increasing of manganese in the soil. The highest content of iron and manganese of maize crop was obtained at a dose of 6.0 tons ha-1 of organic matter without calcite application.


2016 ◽  
Vol 1 (1) ◽  
pp. 808-815
Author(s):  
Sugrahadi Ahmad Aprianto ◽  
Yunasri Usman ◽  
Asril Asril

Abstrak: Penelitian ini telah dilaksanakan di Laboratorium Ilmu Nutrisi dan Makanan Ternak Program Studi Peternakan Fakultas Pertanian Universitas Syiah Kuala dan dilanjutkan dengan pengiriman sampel penelitian ke Laboratorium Ternak Perah Institut Pertanian Bogor (IPB) untuk pengujian kecernaan secara in vitro. Tujuan dari penelitian ini adalah menguji kecernaan secara in vitro complete feed berbahan dasar ampas sagu dengan teknik fermentasi yang berbeda.Pembuatan complete feed disusun sesuai dengan kebutuhan ternak domba. Penelitian ini menggunakan Rancangan Acak lengkap (RAL) dengan 3 perlakuan dan masing-masing perlakuan terdiri atas 5 ulangan. Perlakuan 1 (kontrol) adalah complete feed tanpa penambahan Saus Burger Pakan (SBP) dan tanpa fermentasi, perlakuan 2 adalah ampas sagu difermentasi dengan SBP selama 14 hari kemudian dicampur menjadi complete feed dan difermentasi hingga 21 hari, sedangkan perlakuan 3 adalah ampas sagu + complete feed kemudian difermentasi dengan SBP selama 21 hari. Parameter yang diamati dalam penelitian ini adalah pH, Bahan Kering (BK), Bahan Organik (BO), Koefisien Cerna Bahan Kering (KCBK), dan Koefisien Cerna Bahan Organik (KCBO). Hasil pengamatan menyimpulkan bahwa, teknik fermentasi yang berbeda berpengaruh sangat nyata (P0,01) terhadap semua parameter yang diamati, yaitu pH, BK, BO, KCBK dan KCBO. Dari hasil penelitian dapat disimpulkan bahwa nilai kecernaan complete feed berbahan dasar ampas sagu dipengaruhi oleh perbedaan teknik fermentasi. Digestibility Evaluation In Vitro Complete Feed Fermentation Sago Residues Based with Different Fermentation Techniques Abstract: This research was conducted at the Laboratory of Nutrition and Feed Studies, Program study Animal Husbandry, Faculty of Agriculture, University of Syiah Kuala and continued with the delivery of sample to the Laboratory of Dairy Cattle Institute Pertanian Bogor (IPB) for testing of in vitro digestibility. The purpose of this study was to test in vitro digestibility of complete feed made from  the residues of sago with different fermentation techniques. Complete feed was arranged based on the needs of sheep. This study used a complete randomized design (CRD) with 3 treatments and each treatment consisted of 5 replicates. Treatment 1 (control) was a complete feed without the addition of Saus Burger Pakan (SBP) and without fermentation, treatment 2 was sago residu  fermented with SBP for 14 days and then mixed into a complete feed and fermented up to 21 days, whereas treatment 3 was the sago residue + complete feed then fermented with SBP for 21 days. The parameters observed in this study were pH, Dry Matter (BK), Organic Matter (BO), Dry Matter digestibility coefficients (KCBK), and Organic Matter Digestibility Coefficient (KCBO). The result influence showed that, different fermentation technique was significantly (P 0.01) all parameters, namely pH, BK, BO, KCBK and KCBO. It can be concluded that the digestibility of complete feed made from sago residue influenced by differences in fermentation techniques


2020 ◽  
Vol 17 (1) ◽  
pp. 45
Author(s):  
Tri Astuti

This research aimed to study effect of Corn cob fermentation using Panerochaeta chrysosporium with adding the different sources of carbohydrat on the digestibility of dry matter, organic matter, and crude protein by in-vitro methode. A Completely Randomized Design  with 4 treatments and  4 replications. The treatments  were : P0 = fermented corncobs + without carbohydrt, P1 = fermented corncobs + 10% rice bran; P2 = fermented corncobs + tapioca flour 10%; P3 = corncob fermented + molasses 10%. The results of this study shown a very significant affected  (P <0.01) on the digestible of dry matter and organic matter but no affected (P> 0.05) on crude protein digestibility. The best results  digestibility of dry matter (66.98%) on the  P3 treatment adding  molasses, and  the highest digestibility of organic matter (67.49%) on the P3 treatment too, but the highest digestibility of  crude protein (51.37%)  in P1 treatment (adding by rice bran)


1971 ◽  
Vol 22 (5) ◽  
pp. 787 ◽  
Author(s):  
GD Brown ◽  
JC Radcliffe

Twenty experimental silages were made from seven pasture species at different stages of maturity. In vivo dry matter, organic matter, and energy digestibilities of the silages were determined at restricted and ad libitum intake levels on standardized pairs of Merino wethers. In vitro dry matter digestibility determinations were made on ground frozen silage and ground oven-dried silage with and without a correction for the loss of volatile compounds during drying. Corrected in vitro dry matter digestibilities of ground oven-dried silage for a 48 hr rumen liquor digestion time gave the most accurate prediction of in vivo dry matter (r = 0.88), organic matter (r = 0.86), and energy (r = 0.74) digestibilities for restricted intake levels. In vitro digestibilities of ground frozen silage more accurately predicted in vivo digestibilities than did in vitro digestibilities of ground oven-dried silage without the correction for volatile losses. In vivo dry matter and organic matter digestibilities were highly correlated (v = 0.97), but both were poorly correlated with in vivo energy digestibilities. In vitro digestion of ground oven-dried silage with a correction for the volatile compounds lost during drying is recommended as a suitable routine determination for predicting the in vivo digestibility of silage.


Author(s):  
Chabib B.H ◽  
Sri Rahayu ◽  
Muhamad Bata

This experiment investigated various substrate growth of bamboo stems microbes (BSM) in rice straw fermentation and its effect on dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N and VFA concentration. Materials were rumen fluid of beef cattle from the slaughterhouse. Basal diets consisted of rice straw and concentrate with ratio of 40 : 60 (% dry matter) were administered to five treatments namely P0 = untreated rice straw (control), P1 = Ammoniated rice straw,  fermented rice straw using microbial bamboo stems grown on ammoniated rice straw (P2), rice bran (P3) and cassava waste (P4). The experiment used in vitro method was designed with Completely Randomized Design and the data were analyzed variance. The results showed that the treatment had no significant effect on dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N and VFA concentration (P>0,05). The fermentation process of microbial bamboo stems in rice straw with a level of 10% with different substrate did not able to  increase the digestibility and products fermentation, but there tends that the rice bran and substrates can increase DMD and N-NH3 concentration


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