scholarly journals Effects of Double Layer Centrifugation on the Improvement of Sperm Quality in Dogs: A Comparative Note among Different Breeds

2021 ◽  
pp. 1-12
Keyword(s):  
Double Layer ◽  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Single Layer ◽  
Progressive Motility ◽  
Prolonged Contact ◽  
And Storage ◽  
Fresh Semen

The evaluation of sperm quality in the laboratory is essential to improve efficiency in assisted reproduction. As in other species, for the dog there are reports that prolonged contact of sperm with some components of seminal plasma is associated with decreased motility and sperm viability. Thus, the centrifugation is a technique widely used to concentrate the spermatozoa and eliminate the supernatant. The purpose of this study was to evaluate the effect of double layer centrifugation on the percentages of total sperm motility and progressive sperm motility of the dog’s semen submitted to the dilution, single layer centrifugation, cooling and storage at 5 °C for 24 and 48 hours. For this purpose, ejaculates of 30 healthy male dogs were evaluated, by taking into account the comparison among the conventional sperm parameters (ejaculate volume, sperm concentration, total sperm motility and sperm progressive motility). The semen samples were examined in standard baseline condition of fresh semen (FS), after dilution (AD), after dilution and single layer centrifugation (SLC), after double layer centrifugation (DLC). According to the different time points, the semen samples were evaluated in baseline conditions, immediately after their collection at (T0), at 24 h (T24) and at 48 h (T48), to evaluate the effect of different treatments on the semen’s quality. Results showed a significant effect of double layer centrifugation on the improvement of total sperm motility and progressive sperm motility percentages of dogs. The use of cooling fresh semen soon after the double layer centrifugation will improve the semen quality up to 48h, with a special emphasis for the percentages of total sperm motility and sperm progressive motility, adding an alternative technical approach to reproductive performance in male breeding dogs.

scholarly journals Cryopreservation and quality assessment of boar semen collected from bulk samples

2019 ◽  
Vol 64 (No. 5) ◽  
pp. 209-216 ◽  
Author(s):  
R Ratchamak ◽  
T Vongpralub ◽  
W Boonkum ◽  
V Chankitisakul
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Bulk Sample ◽  
Energy Status ◽  
Semen Volume ◽  
Boar Semen ◽  
Live Sperm ◽  
Fresh Semen

The purpose of this study was to examine sperm quality after cryopreservation of ejaculates collected as a bulk sample, which is routinely part of semen collection, and to compare this quality with the sperm-rich fraction in boars. Ejaculates were collected as sperm-rich fractions (SRF) and bulk samples (BE) using a gloved-hand technique. Fresh semen quality in terms of semen volume, sperm concentration, total sperm motility and pH were conventionally evaluated. Then, semen was cryopreserved using the liquid nitrogen vapour method. The post-thaw sperm quality was evaluated by assessing sperm motility, live sperm with normal apical ridge and high mitochondrial energy status, lipid peroxidation was evaluated using CASA and fluorescent multiple staining and MDA levels were determined using a spectrophotometer, respectively. In terms of fresh semen quality, sperm motility in fresh semen did not differ significantly between the two groups. The treatment with the greater mean volume (BE; P < 0.05) had a lower mean sperm concentration (P < 0.05); meanwhile, the mean ejaculate pH collected as BE was more basic compared with SRF (P < 0.05). However, there were no significant post-thaw quality changes between sperm-rich fractions and bulk samples of semen. In conclusion, ejaculates can be collected as bulk samples without the need to classify fractions for boar semen cryopreservation.

scholarly journals Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4493
Author(s):  
Ronan Maciel Marcos ◽  
Giovano Neumann ◽  
Cesar Pereira Rebechi de Toledo ◽  
João Marcos Sena ◽  
Gilmar Baumgartner ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Storage Temperature ◽  
Sperm Concentration ◽  
Plasma Osmolality ◽  
Storage Period ◽  
Sperm Velocity ◽  
Powdered Milk ◽  
Sperm Activation ◽  
And Storage ◽  
Fresh Semen

<p>This study describes the seminal and spermatic characteristics of fresh semen of <em>Steindachneridion melanodermatum </em>and investigates the effects of dilution, temperature, and storage period on its spermatic parameters. Sperm samples were collected from nine hormonally-induced males. The following parameters in fresh sperm were analyzed: seminal plasma osmolality (OSM), seminal pH, sperm motility (MOT), sperm velocity (SV) (including sperm curvilinear velocity (CVV), sperm straight-line velocity (SLV), and sperm average path velocity (APV)), total time of sperm motility (TEMP), sperm concentration (CONC), and index of sperm normality (NORM). Sperm samples from each male were diluted in a solution containing 5% fructose and 5% powdered milk, and stored at 10°C and 25°C. The same was carried out for sperm samples not subjected to dilution. From these samples, MOT, CVV, SLV, APV, SV, and TEMP were measured after 0 h, 5 h, 9 h, 18 h, 27 h, 36 h, 45 h, and 54 h. Males released 11.74 ± 5.38 mL of sperm, with an osmolality of 258.78 ± 29.36 mOsm.kg-1 and pH of 7.11 ± 0.31. The sperm presented a MOT of 99.86 ± 0.31% at a concentration of 1.03 × 1010 ± 3.65 × 109 spermatozoa.mL-1 with CVV of 185.58 ± 14.11 ?m.s-1, SLV of 49.15 ± 4.66 ?m.s-1, APV of 87.02 ± 4.13 ?m.s-1, SV of 106.52 ± 4.45 ?m.s-1, TEMP of 79.31 ± 5.62 s, NORM of 75.81 ± 5.71%. The results indicate that sperm motility, sperm velocity, and total time of sperm activation were affected by dilution, storage temperature, and storage period (p &lt; 0.05). Procedures for semen storage should be performed with undiluted sperm cooled at 10°C, or kept undiluted at 25°C for up to 27 h.</p>

23 Sperm quality of Pure Spanish stallions is affected by inbreeding coefficient and age

2020 ◽  
Vol 32 (2) ◽  
pp. 137
Author(s):  
Y. Pirosanto ◽  
M. Valera ◽  
A. Molina ◽  
J. Dorado ◽  
S. Demyda-Peyrás
Keyword(s):  
Sperm Motility ◽  
Inbreeding Depression ◽  
Negative Correlation ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Inbreeding Coefficient ◽  
Semen Parameters ◽  
Computer Assisted

Inbreeding depression, a genetic condition produced by the mating of close-related individuals, has been associated with a reduction of fertility in several species. However, a loss in sperm quality was also associated with age. In horses, the few existing reports have described a tendency of both parameters to produce a negative effect on sperm quality. However, those reports were performed using a subjective evaluation of sperm motility. In the present study, a total of 692 ejaculates from 86 Pure Spanish stallions (PRE), aged between 3 and 22 years, were evaluated using a computer-assisted methodology to determine the effect of inbreeding in four semen parameters: free-gel volume (V), sperm concentration (C, by haemocytometer), and total (TM) and progressive (PM) sperm motility (by Spermvision sperm class analyser; Minitube). The inbreeding coefficient (F) was estimated using 300 000 PRE pedigree records approximately (minimum pedigree depth, eight equivalent complete generations; range, between 1 and 30.1%). Stallion, age, ejaculate, and season of semen collection were the variables included in the statistical model (general linear model), with ejaculate and season being the variables with a major effect (by variance components analysis). Our results showed that sperm concentration (r=−0.18; P&lt;0.0001) and volume (to a lesser extent) were reduced with advancing age, both showing a major decline after 15 years of age. To the contrary, sperm motility was not affected by age of the stallion. We also found a negative correlation between the inbreeding coefficient and ejaculate volume (r=−0.14; P&lt;0.001), with a marked decrease seen when F was between 7 and 20%. Also, a negative correlation was observed in PM (r=−0.08; P&lt;0.05), although to a lower extent. Conversely, C and TM were not affected by inbreeding depression (P&gt;0.05). In conclusion, our results demonstrated that high levels of inbreeding can compromise severely the sperm quality of the PRE stallion, which, subsequently, may have a negative influence on fertility. Ongoing studies using genomic data will help to detect genetic variants associated with stallion semen quality and how it is influenced by inbreeding in specific genomic regions.

scholarly journals Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4493
Author(s):  
Ronan Maciel Marcos ◽  
Giovano Neumann ◽  
Cesar Pereira Rebechi de Toledo ◽  
João Marcos Sena ◽  
Gilmar Baumgartner ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Storage Temperature ◽  
Sperm Concentration ◽  
Plasma Osmolality ◽  
Storage Period ◽  
Sperm Velocity ◽  
Powdered Milk ◽  
Sperm Activation ◽  
And Storage ◽  
Fresh Semen

This study describes the seminal and spermatic characteristics of fresh semen of Steindachneridion melanodermatum and investigates the effects of dilution, temperature, and storage period on its spermatic parameters. Sperm samples were collected from nine hormonally-induced males. The following parameters in fresh sperm were analyzed: seminal plasma osmolality (OSM), seminal pH, sperm motility (MOT), sperm velocity (SV) (including sperm curvilinear velocity (CVV), sperm straight-line velocity (SLV), and sperm average path velocity (APV)), total time of sperm motility (TEMP), sperm concentration (CONC), and index of sperm normality (NORM). Sperm samples from each male were diluted in a solution containing 5% fructose and 5% powdered milk, and stored at 10°C and 25°C. The same was carried out for sperm samples not subjected to dilution. From these samples, MOT, CVV, SLV, APV, SV, and TEMP were measured after 0 h, 5 h, 9 h, 18 h, 27 h, 36 h, 45 h, and 54 h. Males released 11.74 ± 5.38 mL of sperm, with an osmolality of 258.78 ± 29.36 mOsm.kg-1 and pH of 7.11 ± 0.31. The sperm presented a MOT of 99.86 ± 0.31% at a concentration of 1.03 × 1010 ± 3.65 × 109 spermatozoa.mL-1 with CVV of 185.58 ± 14.11 ?m.s-1, SLV of 49.15 ± 4.66 ?m.s-1, APV of 87.02 ± 4.13 ?m.s-1, SV of 106.52 ± 4.45 ?m.s-1, TEMP of 79.31 ± 5.62 s, NORM of 75.81 ± 5.71%. The results indicate that sperm motility, sperm velocity, and total time of sperm activation were affected by dilution, storage temperature, and storage period (p < 0.05). Procedures for semen storage should be performed with undiluted sperm cooled at 10°C, or kept undiluted at 25°C for up to 27 h.

scholarly journals Effect of Macrophages in Semen on Sperm Quality

2020 ◽  
Author(s):  
Gangxin Chen ◽  
Beihong Zheng
Keyword(s):  
Cell Membrane ◽  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Significant Negative Correlation ◽  
High Group ◽  
Cross Sectional ◽  
Cell Membrane Integrity

Abstract Background: This was a cross-sectional study in China, we analyzed the levels of macrophages(Mφ) in semen. The study evaluated the influence of the levels of Mφ in semen on sperm quality.Methods: The subjects were 78 males between 25 and 35 years old. The samples were divided into a low group(Mφ<6×105/ml) and a high group (Mφ>6×105/ml). Evaluation included consideration of the influencing factors of male semen quality, macrophage concentration, sperm motility, morphology, membrane integrity DNA fragmentation index (DFI), anti-sperm antibodies (AsAb), IL-10, and IL-12 in semen.Results: There was no difference in the physical or chemical indices of the semen, sperm concentration, AsAb, IL-10, or IL-12 between the two groups (P>0.05). The percentage of sperm forward motility (PR%), the rate of normal sperm shape, and the integrity of cell membranes in the low group were higher than those in the high group (P<0.05), while the percentage of sperm inactivity (IM%), the rate of sperm head deformity, the rate of deformity in the neck and middle segment, the sperm malformation index (SDI), the abnormal sperm index (TZI), and the sperm DFI in the low group were lower than those in the high group (P<0.05). The concentration of Mφ in the semen was linearly correlated with sperm concentration, sperm PR%, IM%, sperm normal shape rate, head deformity rate, neck and middle deformity rate, SDI, TZI, sperm DFI, and sperm cell membrane integrity (P<0.05), but there was no linear correlation with IL-10 or IL-12 (P>0.05).Conclusions: The concentration of Mφ in semen had no significant correlation with semen volume or sperm concentration, but it did have a significant negative correlation with sperm motility, sperm morphology, cell membrane integrity, and DNA breakage rate. There was no significant correlation with the concentration of IL-10 or IL-12.

scholarly journals The quality of buck semen after feed additive minoxvit administration

2021 ◽  
Vol 19 (1) ◽  
pp. 87
Author(s):  
Anita Hafid ◽  
Riasari Gail Sianturi ◽  
Diana Andrianita Kusumaningrum ◽  
Yeni Widiawati ◽  
Anneke Anggraeni ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Positive Influence ◽  
Feed Additive ◽  
Concentration Data ◽  
Randomized Design ◽  
Microscopic Evaluation

<p class="MDPI17abstract"><strong>Objective: </strong>Reproduction and nutritional status is closely related. Nutritional deficiency or insufficiency directly affects reproductive efficiency. Deficiency of nutrition could affect the sperm quality and the ability to fertilize. The objective of this study was to evaluate the effect of feed additive (Minoxvit) on semen quality of buck.</p><p class="MDPI17abstract"><strong>Methods: </strong>This study used 6 sexually mature bucks, aged 1.5 years old. The bucks were fed daily with 3 kg of freshly chopped king grass, 1 kg of Legume (<em>Calliandra</em> sp.), and 500 g of concentrate. Three bucks were given Minoxvit by 1.25 g/day in the concentrate, while three other bucks were considered as control. Semen was evaluated once a week for 49 days. Semen was evaluated macroscopically and microscopically. The macroscopic evaluation consisted of volume, color, and consistency of semen, while microscopic evaluation consisted of mass motility, sperm motility, viability, and sperm concentration. Data were analyzed using Completely Randomized Design with Tukey test.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The result showed significantly different (<em>P</em>&lt;0,05) in which bucks semen in Minoxvit addition group had higher semen volume (0.75 ml vs 0.54 ml), mass motility (3.32 vs 2.67), sperm motility (70% vs 58 %), sperm viability (86.67% vs 79.19%), and sperm consentration (2,6x10<sup>9</sup> mL vs 1,7x10<sup>9</sup> mL).<strong></strong></p><p><strong>Conclusions: </strong>This study concludes that the addition of Minoxvit has a positive influence on the quality of buck sperms providing volume, mass motility, individual motility, viability, and concentration of the sperm.</p>

scholarly journals Pengaruh Suplementasi Vitamin E, Mineral Selenium dan Zink Terhadap Konsumsi Nutrien, Produksi dan Kualitas Semen Sapi Simental

2014 ◽  
Vol 14 (1) ◽  
pp. 6-16
Author(s):  
Fitrah Khairi ◽  
Anis Muktiani ◽  
Yon Supri Ondho
Keyword(s):  
Vitamin E ◽  
Sperm Motility ◽  
Rainy Season ◽  
Dry Matter ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Cement Production ◽  
Semen Volume ◽  
Nutrient Consumption ◽  
Fresh Semen

(Effect of supplementation of vitamin E, Selenium and Zinc mineral against to nutrient consumption, production and quality cement simental cow)ABSTRACT. Simental bulls often experience a decline in production and semen quality in the rainy season, which have a higher humidity and high rainfall. This study aims to maintain nutrient consumption, production and semen quality Simental bulls during the rainy season through the supplementation of Vitamin E, Selenium and Zinc Minerals. The study was conducted in July-December 2012 at the Artificial Insemination Center (BIB) Unggaran. The research material used in this study were 12 males Simental cows BIB Unggaran grouped by age. The experimental design used in this study is a randomized block design (RBD) with 3 treatments T1 (ration + Vit. E), T2 (ration + Vit. E + Se), T3 (ration + Vit. E + Se + Zn) and 4 groups as replicates. Parameters measured were dry matter intake and nutrient consumption, production and semen quality. Cement production was measured from fresh semen volume during the study, whereas semen quality measured motility and concentration of spermatozoa from fresh semen. Data consumption of dry matter and nutrient intake obtained were processed using analysis of variance, followed by Duncan test. While data on production and semen quality were analyzed descriptively. The results showed that the combination of vitamin E supplementation, minerals Selenium and Zinc did not affect dry matter intake, total digestible nutriens and crude protein, but resulted in a decrease in the percentage of shelter, semen volume, sperm motility and concentration smaller than before treatment. Treatment T1, T2 and T3 resulted in a decrease in the number of shelter respectively 41,55%, 19,56% and 13,63% compared to before treatment, whereas a decrease in semen volume unchanged at 44,9 %, 43,7% and 40,99%. Sperm motility due to treatment T1, T2 and T3 respectively decreased by 55,87%, 22,10% and 13,63% compared to before treatment. In line with sperm motility, sperm concentration in treatment T , T2 and T3, respectively decreased by 49,16%, 22,85% and 14,88%. The conclusion is a combination of vitamin E supplementation, minerals Selenium and Zinc can prevent the decline in cement production, sperm motility and sperm concentration Simental bulls during the rainy season.

Association between kallikrein-related peptidases (KLKs) and macroscopic indicators of semen analysis: their relation to sperm motility

2009 ◽  
Vol 390 (9) ◽  
Author(s):  
Nashmil Emami ◽  
Andreas Scorilas ◽  
Antoninus Soosaipillai ◽  
Tammy Earle ◽  
Brendan Mullen ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Differentially Expressed ◽  
Viscosity Data ◽  
Clinical Value

Abstract Human kallikrein-related peptidases (KLKs) are a family of proteases, the majority of which are found in seminal plasma and have been implicated in semen liquefaction. Here, we examined the clinical value of seminal KLKs in the evaluation of semen quality and differential diagnosis and etiology of abnormal liquefaction and/or viscosity. KLK1–3, 5–8, 10, 11, 13, and 14 were analyzed, using highly specific ELISA assays. Samples were categorized into four clinical groups, according to their state of liquefaction and viscosity. Data were compared between the clinical groups and in association with other parameters of sperm quality, including number of motile sperms, straight line speed, sperm concentration, volume, pH, and patient age. Seminal KLKs were found to be differentially expressed in the four clinical groups. Combination of KLK2, 3, 13, and 14 and KLK1, 2, 5, 6, 7, 8, 10, 13, and 14 showed very strong discriminatory potential for semen liquefaction and viscosity, respectively. Liquefaction state was associated with several parameters of sperm motility. Finally, KLK14 was differentially expressed in asthenospermic cases. In conclusion, the expression level of several seminal plasma KLKs correlates with liquefaction and viscosity indicators of semen quality and may aid in their differential diagnosis and etiology.

scholarly journals Influence of age on sperm characteristics evaluated by light and electron microscopies

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Giulia Collodel ◽  
Fabio Ferretti ◽  
Maurizio Masini ◽  
Giacomo Gualtieri ◽  
Elena Moretti
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Study Data ◽  
Wine Consumption ◽  
Electron Microscopies ◽  
Urogenital Infection ◽  
The Impact

AbstractThe impact of male aging on male fertility has only recently become of interest to the scientific community. This study aims to assess the relationship between age and fertility among a sample of men, considering the individual and pathological characteristics. In this retrospective study data of semen analysis and medical history of 1294 Italian male patients were considered. Semen analysis was performed by light microscopy and transmission electron microscopy mathematically elaborated. A generalized linear model was used to explore the influence of male age on semen quality, considering as confounders wine consumption, smoking habits, presence of varicocele, consanguinity and positive semen bacteriological analysis and urethral swab. The mean age of the participants was 36.41 ± 6.379. Male aging without impact of confounders was correlated with a decrease in sperm concentration and motility and an increased in sperm necrosis. Sperm concentration and progressive motility were negatively related to the presence of confounders as wine consumption (sperm motility), urogenital infection (sperm concentration and motility), varicocele (sperm concentration) and consanguinity (sperm motility). Urogenital infection, varicocele and consanguinity positively correlated with sperm necrosis. The most important finding was the observation of a negative effect of male aging on sperm parameters such as concentration, motility, and viability. It is possible to hypothesize age-dependent changes of testicular environment, probably related to reactive oxygen species production. The demonstration, in a large sample of patients, that aging influences sperm quality strongly motivates further research focused on the mechanisms involved in this phenomenon and its effects on offspring fitness.

scholarly journals Association of Anxiety With Sperm Quality: a Multicentre Cross-sectional Study in China During the COVID-19 Outbreak

2021 ◽  
Author(s):  
Yongfeng Wang ◽  
Liting He ◽  
Yunyi Yang ◽  
Lihui Tu ◽  
Jigao Yang ◽  
...  
Keyword(s):  
Linear Regression ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Cross Sectional Study ◽  
Anxiety Symptoms ◽  
Quality Analysis ◽  
Cross Sectional ◽  
Progressive Motility

Abstract Objectives: There is no report about association of anxiety on sperm quality during the COVID-19 epidemic. Purpose of this study was to investigate the relationship between anxiety and sperm quality during the COVID-19 outbreak in China.Methods: 896 sperm donors from 7 sperm banks in China were investigated, and passed the screening for sperm donation between 23 January 2020 and 8 June 2020. Semen quality analysis follows the standards of the WHO Fifth Edition Human Semen Examination and Processing Manual. Anxiety symptoms were assessed by the Generalized Anxiety Disorder Scal-7(GAD-7). Logistic regression and linear regression were used to identify the association between anxiety level and sperm quality.Results: Participants with anxiety status (N = 155; 17.3%) had lower sperm concentration (56.81 ± 32.70 vs. 47.21 ± 30.76 [106/mL]), sperm volume (3.58 ± 1.65 vs. 3.04 ± 1.69 [mL], P < 0.001), total sperm count (7.29 ± 1.23 vs. 6.56 ± 1.63 [106], P < 0.001), and progressive motility (50.86 ± 12.45 vs. 38.50 ± 17.00 [%], P < 0.001) than those without anxiety. Linear regression showed that anxiety symptoms was significantly associated with 8.13 (95% confidence interval [CI]: 2.51, 13.75), 0.49 (0.20, 0.79) ,51.05 (23.43, 78.68) and 12.15 (95% CI: 23.43, 78.68), reduction in sperm concentration, semen volume, and total sperm count and progressive motility respectively.Conclusions: This study revealed that anxiety symptoms were negatively associated with semen quality, and interaction was discovered between debt stress and anxiety.

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