Determination of the water-extractable fraction of iron in selected medicinal plant raw materials: Folium Menthae, Folium Urticae and Folium Salviae

The article is devoted to the development of new and perspective for the State Pharmacopoeia of the Russian Federation (SP RF) techniques for determining of the moisture (loss on drying) of medicinal plant raw materials of the morphological group “Fruit” and medicinal plant preparations by the use of infrared thermogravimetric (IRTGM) method. The techniques for determination of moisture (loss on drying) IRTGM-method as exemplified by the fruit of the following medicinal plants: Silybum marianum (L.) Gaertn., Rosa cinnamomea L. and Anethum graveolens L. were developed. The comparable results for determining of the moisture (loss on drying) of the medicinal plant raw materials of investigated plants were obtained by means of two methods: IRTGM and pharmacopoeial method, which allow us to recommend the developed techniques for inclusion into the relevant monographs of the State Pharmacopoeia of the Russian Federation.

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Isolation and determination of some physical and chemical properties of polysaccharide from black elderberry flowers

Herald of Tver State University. Series: Biology and Ecology ◽

10.26456/vtbio163 ◽

2020 ◽

pp. 95-101

Author(s):

Игорь Анатольевич Сычев ◽

Татьяна Викторовна Алимкина

Keyword(s):

Medicinal Plant ◽

Quantitative Determination ◽

Raw Materials ◽

Chemical Properties ◽

Physical And Chemical Properties ◽

Plant Raw Materials ◽

Physical And Chemical ◽

And Chemical Properties

Предложенный метод количественного определения восстанавливающих моносахаридов в полисахариде позволяет проводить стандартизацию лекарственного растительного сырья цветков бузины черной. На способ стандартизации лекарственного растительного сырья цветков бузины черной по содержанию восстанавливающих моносахаридов в полисахариде оформлено рационализаторское предложение № 1373 от 29.02.2016 г. РязГМУ. The proposed method for the quantitative determination of reducing monosaccharides in a polysaccharide makes it possible to standardize medicinal plant raw materials of black elderberry flowers. On the method of standardization of medicinal plant raw materials of black elderberry flowers in terms of the content of reducing monosaccharides in the polysaccharide, a rationalization proposal No. 1373 dated February 29, 2016 RyazSMU was issued.

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Validation of the HPLC-UV Method for Quantitative Determination of Steroid Sapogenin Diosgenin from Hay Fenugreek Seeds, Trigonella Foenum-graecum L.

Drug development & registration ◽

10.33380/2305-2066-2020-9-4-107-114 ◽

2020 ◽

Vol 9 (4) ◽

pp. 107-114

Author(s):

A. E. Sukhanov ◽

A. N. Stavrianidi ◽

E. D. Kubasova ◽

A. S. Panasyuk ◽

O. V. Buyuklinskaya

Keyword(s):

Medicinal Plant ◽

Quantitative Determination ◽

Plant Extracts ◽

Raw Materials ◽

Biologically Active ◽

Fenugreek Seeds ◽

Plant Raw Materials ◽

Object Of Study ◽

Trigonella Foenum Graecum L

Introduction. Modern pharmacognostic research is aimed at searching for plant biologically active individual compounds (hereinafter referred to as RBAIS) isolated from plant extracts.Aim. Validation of HPLC-UV quantitative determination of sapogenin diosgenin in plant extracts from fenugreek seeds.Materials and methods. The object of study was raw materials-fenugreek seeds produced as medicinal plant raw materials by LLC «Sage» (Irkutsk). Validation of the method was carried out according to the parameters: specificity, linearity, correctness, precision in accordance with the requirements of SP XIV. One series of medicinal plant raw materials was used for the analysis, such as serial number – 010117, release date – 15 february 2017.Results and discussion. Validation characteristics were determined and their compliance with the necessary acceptance criteria was experimentally confirmed.Conclusion. It is established that the developed method of identification and quantitative determination of diosgenin in fenugreek seed extracts by HPLC-UV is correct, precise, specific and linear in the analytical field.

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Interrelationships among selected essential elements in medicinal plant raw materials and their water-extractable forms

Herba Polonica ◽

10.2478/hepo-2013-0011 ◽

2013 ◽

Vol 59 (2) ◽

pp. 46-58

Author(s):

Paweł Konieczyński ◽

Marek Wesołowski

Keyword(s):

Medicinal Plant ◽

Raw Materials ◽

Principal Component ◽

Essential Elements ◽

Water Soluble ◽

Plant Materials ◽

Plant Raw Materials ◽

Plant Parts ◽

Plant Samples ◽

Water Extractable

Summary In the study, the relationships were investigated among N, P, Fe, Zn, Mn and Cu, in medicinal plant raw materials (herbal bags) and their water-soluble chemical forms in infuses. 42 independent samples of plant materials were chosen, represented by different morphological plant parts as herbs, leaves, flowers and fruits. The elements were determined by UV/Vis spectrometry (non-metals), and FAAS (metallic elements) after previous mineralization of plant samples (total concentrations), and directly in herbal teas (water-extractable forms). Most frequently the correlations between non-metals and Zn and Mn occurred, both between their total contents the water-extractable forms. Statistically significant correlations were also found in pairs: Zn-Mn, Fe-Zn, Mn-Fe, and Cu-Zn. Application of multivariate analysis revealed that cluster analysis grouped the studied samples into clusters with similar levels of the analyzed elements, and principal component analysis allowed the identification of water-extractable Zn, P-PO4 and water-extractable Cu as the most crucial factors determining the differentiation of the studied plant samples.

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METHODS FOR QUANTITATIVE DETERMINATION OF TOTAL FLAVONOIDS IN QUERCUS ROBUR L. BUDS

Pharmacy & Pharmacology ◽

10.19163/2307-9266-2021-9-5-356-366 ◽

2021 ◽

Vol 9 (5) ◽

pp. 356-366

Author(s):

N. A. Ryabov ◽

V. M. Ryzhov ◽

V. A. Kurkin

Keyword(s):

Medicinal Plant ◽

Quantitative Determination ◽

Ethyl Alcohol ◽

Quercus Robur ◽

Raw Materials ◽

Uv Spectrophotometry ◽

Total Flavonoids ◽

Plant Raw Materials ◽

Wide Range

Currently, the actual task of modern pharmacy is to study the chemical composition and pharmacological properties of plant objects. Within the framework of this concept, it seems interesting to study Quercus robur L. buds. One of the promising groups of biologically active compounds of Quercus robur L. buds are flavonoids. This group of substances has a wide range of a pharmacological activity, which is significant in the creation of new medicines based on medicinal plant raw materials.The aim of the article was to work out methods for quantitative determination of total flavonoids in Quercus robur l. buds.Materials and methods. The research materials were aqueous-alcoholic extracts from Quercus robur L. buds with 70% ethyl alcohol which were analyzed by differential UV spectrophotometry on spectrophotometer “SF 2000” (Russia).Results. The methods for quantitative determination of total flavonoids in Quercus robur L. buds by differential UV spectrophotometry, has been developed using a standard sample of cynaroside at the analytical wavelength of 400 nm. The optimum parameters for the extraction of total flavonoids from Quercus robur L. buds have been determined. They are: the optimum extractant is 70% ethyl alcohol; the “raw material-extractant” ratio is 1:50; the extraction time is 120 min, the degree of atomization is 2 mm.The content of total flavonoids for Quercus robur L. buds has been determined; it varies from 0.27%±0.01 to 0.44%±0.02. These results make possible to recommend the content of total flavonoids for this type of raw materials not less than 0.25% as a lower limit.Conclusion. The data obtained in the course of the experiment, makes it possible to conclude that a further study of Quercus robur L. buds is promising, and it also contributes to the implementation of medicinal plant raw materials “Quercus robur L. buds” in the State Pharmacopoeia (Russia).

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Development of approaches to standardization of black walnut bark

Aspirantskiy Vestnik Povolzhiya ◽

10.17816/2072-2354.2020.20.1.131-136 ◽

2020 ◽

Vol 20 (1-2) ◽

pp. 131-136

Author(s):

Natalya I. Zimenkina ◽

Vladimir A. Kurkin

Keyword(s):

Medicinal Plant ◽

Quantitative Determination ◽

Raw Materials ◽

Juglans Regia ◽

Black Walnut ◽

Juglans Nigra ◽

Raw Material ◽

Plant Raw Materials ◽

Plant Raw Material

Significance. Black Walnut (Juglans nigra L.) is a species of trees of the Walnut family (Juglandaceae). This plant of the genus Walnut (Juglans L.) has not been sufficiently studied unlike other species e.g. Juglans regia L. This medicinal plant raw material is quite perspective, its preparations have antimicrobial, general tonic effect. We can use its leaves, unripe fruit, and the bark. However they are not widely used in medicine. In order to introduce the plants of the Walnut genus to the State Pharmacopoeia of the Russian Federation (RF State Pharmacopoeia), it is necessary to conduct of pharmacognostic studies, to develop product specification file to confirm the identification and quality of medicinal plant raw materials. The aim of this study is to develop a method of quantitative determination of flavonoids in the bark of the black walnut (Juglans nigra L.). Materials and methods. Material of the study was black walnut bark, stocked during the sap flow period (April) in 2018. The bark was skived up to 15 cm long and 23 cm wide. The bark was air-dried with the protection from direct sun light. The end of the drying was checked by the brittleness of the bark. Results. The methods of the quantitative determination of flavonoids in walnut bark has been developed. We used the differential spectrophotometry taking into consideration state standard sample of rutin at the analytical wavelength of 416 nm. The error of a single determination with a confidence level of 95% is 1.20%. Conclusion. We used the developed technique and analyzed a number of samples of black walnut bark. The content of total flavonoids in the plant raw material is 5.13 0.02% (as calculated on rutin). The flavonoid content should be at least 4.0%.

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