Decellularized liver-regenerative 3D printing biomaterials for cell-based liver therapies via a designed procedure combined with papain-containing reagent treatments and supercritical fluids

2021 ◽  
pp. 1-8
Author(s):  
Ching-Cheng Huang
Keyword(s):  
3D Printing ◽  
Thermo Gravimetric Analysis ◽  
Extraction Process ◽  
Gravimetric Analysis ◽  
Collagen Scaffolds ◽  
Scanning Calorimetry ◽  
Porcine Liver ◽  
Good Thermal Stability ◽  
Decellularized Liver ◽  
Liver Collagen

BACKGROUND: The biologic scaffolds derived from decellularized tissues and organs have been successfully developed in a variety of preclinical and/or clinical studies. OBJECTIVE: The new decellularized liver-regenerative 3D printing biomaterials were designed and prepared for cell-based liver therapies. METHODS: An extraction process was employed to remove the tissue and cellular molecules from porcine liver via pretreatment of supercritical fluid of carbon dioxide (ScCO2). Varying porosities of the decellularized liver tissues were created using papain-containing reagent treatments after ScCO2. RESULTS: The resulting liver-regenerative 3D printing biomaterials of decellularized liver collagen scaffolds were characterized by Fourier transform infrared spectroscopy, thermo-gravimetric analysis, differential scanning calorimetry and scanning electron microscopy. CONCLUSIONS: The decellularized liver collagen scaffolds with good thermal stability (>150 °C) were obtained and employed as liver-regenerative 3D printing biomaterials for cell-based liver therapies.

scholarly journals Biocomposites of Bio-Polyethylene Reinforced with a Hydrothermal-Alkaline Sugarcane Bagasse Pulp and Coupled with a Bio-Based Compatibilizer

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2158
Author(s):  
Nanci Vanesa Ehman ◽  
Diana Ita-Nagy ◽  
Fernando Esteban Felissia ◽  
María Evangelina Vallejos ◽  
Isabel Quispe ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
3D Printing ◽  
Water Absorption ◽  
Sugarcane Bagasse ◽  
Thermo Gravimetric Analysis ◽  
Decomposition Temperature ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Cradle To Gate ◽  
Bagasse Pulp

Bio-polyethylene (BioPE, derived from sugarcane), sugarcane bagasse pulp, and two compatibilizers (fossil and bio-based), were used to manufacture biocomposite filaments for 3D printing. Biocomposite filaments were manufactured and characterized in detail, including measurement of water absorption, mechanical properties, thermal stability and decomposition temperature (thermo-gravimetric analysis (TGA)). Differential scanning calorimetry (DSC) was performed to measure the glass transition temperature (Tg). Scanning electron microscopy (SEM) was applied to assess the fracture area of the filaments after mechanical testing. Increases of up to 10% in water absorption were measured for the samples with 40 wt% fibers and the fossil compatibilizer. The mechanical properties were improved by increasing the fraction of bagasse fibers from 0% to 20% and 40%. The suitability of the biocomposite filaments was tested for 3D printing, and some shapes were printed as demonstrators. Importantly, in a cradle-to-gate life cycle analysis of the biocomposites, we demonstrated that replacing fossil compatibilizer with a bio-based compatibilizer contributes to a reduction in CO2-eq emissions, and an increase in CO2 capture, achieving a CO2-eq storage of 2.12 kg CO2 eq/kg for the biocomposite containing 40% bagasse fibers and 6% bio-based compatibilizer.

scholarly journals Lignin: A Biopolymer from Forestry Biomass for Biocomposites and 3D Printing

Materials ◽  
2019 ◽  
Vol 12 (18) ◽  
pp. 3006 ◽  
Author(s):  
Mihaela Tanase-Opedal ◽  
Eduardo Espinosa ◽  
Alejandro Rodríguez ◽  
Gary Chinga-Carrasco
Keyword(s):  
3D Printing ◽  
Radical Scavenging Activity ◽  
Matrix Material ◽  
Thermo Gravimetric Analysis ◽  
Radical Scavenging ◽  
Fused Deposition Modelling ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Biobased Materials ◽  
Fused Deposition

Biopolymers from forestry biomass are promising for the sustainable development of new biobased materials. As such, lignin and fiber-based biocomposites are plausible renewable alternatives to petrochemical-based products. In this study, we have obtained lignin from Spruce biomass through a soda pulping process. The lignin was used for manufacturing biocomposite filaments containing 20% and 40% lignin and using polylactic acid (PLA) as matrix material. Dogbones for mechanical testing were 3D printed by fused deposition modelling. The lignin and the corresponding biocomposites were characterized in detail, including thermo-gravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction analysis (XRD), antioxidant capacity, mechanical properties, and scanning electron microscopy (SEM). Although lignin led to a reduction of the tensile strength and modulus, the reduction could be counteracted to some extent by adjusting the 3D printing temperature. The results showed that lignin acted as a nucleating agent and thus led to further crystallization of PLA. The radical scavenging activity of the biocomposites increased to roughly 50% antioxidant potential/cm2, for the biocomposite containing 40 wt % lignin. The results demonstrate the potential of lignin as a component in biocomposite materials, which we show are adequate for 3D printing operations.

Optically active polymer: synthesis and characterization of new optically active poly (hydrazide-imide)s incorporating L- leucine

e-Polymers ◽  
2007 ◽  
Vol 7 (1) ◽  
Author(s):  
Abdol Reza Hajipour ◽  
Saeed Zahmatkesh ◽  
Arnold E. Ruoho
Keyword(s):  
Thermo Gravimetric Analysis ◽  
Specific Rotation ◽  
Optically Active ◽  
Gravimetric Analysis ◽  
Optically Active Polymer ◽  
Scanning Calorimetry ◽  
Thermo Gravimetric ◽  
Good Thermal Stability ◽  
H Nmr

AbstractThis paper deals with the polycondensation between a chiral diacyl chloride (N,N′-Pyromelliticdiimido-di-L-leucine chloride) and six different dihydrazides. The corresponding poly (hydrazide-imide)s which have been obtained in quantitative yields are moderately soluble in polar aprotic solvents, have good thermal stability and optical activity. The synthetic compounds have been characterized by IR, UV and 1H NMR spectroscopy, elemental analysis and specific rotation. The thermal properties of the polymers (10 and 15) have been studied by thermo gravimetric analysis (TGA) and differential scanning calorimetry (DSC).

Fabrication of modified-release custom-designed ciprofloxacin tablets via fused deposition modeling 3D printing

2020 ◽  
Vol 4 (1) ◽  
pp. 17-27
Author(s):  
Nasir Abbas ◽  
Nadia Qamar ◽  
Amjad Hussain ◽  
Sumera Latif ◽  
Muhammad Sohail Arshad ◽  
...  
Keyword(s):  
3D Printing ◽  
Drug Release ◽  
Fused Deposition Modeling ◽  
Structural Characteristics ◽  
Thermo Gravimetric Analysis ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Ciprofloxacin Hydrochloride ◽  
Fused Deposition ◽  
Degradation Temperature

Aim: The aim of the present work was to fabricate customized tablets of ciprofloxacin hydrochloride through 3D printing for optimized dosing. Materials & methods: A hot melt extrusion technique was employed to produce polyvinyl alcohol filaments with differing strengths of ciprofloxacin hydrochloride. Drug-loaded filaments were characterized for mechanical strength, thermal behavior and structural characteristics prior to printing of tablets by varying the infill percentage. Final formulations were evaluated for drug release profiles. Results: The prepared formulations contained 15–20% drug. The drug release patterns of different formulations were found to be reliant on infill percentage. Differential scanning calorimetry and thermo-gravimetric analysis confirmed that degradation temperature of drug is way above the printing temperature. Conclusion: This work is potentially significant for optimized antibiotic dosing, which in turn leads to enhanced clinical outcome.

Temperature-dependent rheological properties of viscoelastic polymer based flexible abrasive media for finishing process

2021 ◽  
pp. 095440892098790
Author(s):  
Santhosh Kumar S ◽  
Somashekhar S Hiremath
Keyword(s):  
Rheological Properties ◽  
Loss Modulus ◽  
Thermo Gravimetric Analysis ◽  
Testing Machine ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Good Thermal Stability ◽  
Viscoelastic Polymer ◽  
Finishing Process ◽  
The Media

The objective of the present investigation is to develop and study the thermal and rheological properties of the in-house developed flexible abrasive media. It is a mixer of viscoelastic polymer and plasticizer mixed with micro-abrasive particles, used for removing debris and irregularities present on internal and external complex surfaces used in the aerospace, automotive and medical industries. This paper presents, morphology of the developed abrasive media is captured using the Scanning Electron Microscope (SEM), thermal properties of the abrasive media are studied using Thermo Gravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) analysis along with tensile properties of the abrasive media ascertained using the universal testing machine. The rheological properties of the media play a major role in media flow over the intricate shapes and removing the material from the surface. To understand the media behavior, further rheological properties such as viscosity, shear stress, storage modulus, loss modulus, loss tangent, and complex viscosity are ascertained with varying shear rate and frequency at different temperature using rheometer. The TGA and DSC results shows abrasive media has good thermal stability and possibility of using the media for finishing process under lower temperature <100 °C. Rheological studies shows the shear thinning behaviour of the media and elastic solid behavior (G′ > G′′) at different temperatures which is suitable for finishing the complex internal and the external features efficiently and effectively. The developed abrasive media is used to finish the hydraulic components which are difficult to access with conventional tools and the results of finishing process are presented.

scholarly journals VO2(B) conversion to VO2(A) and VO2(M) and their oxidation resistance and optical switching properties

2016 ◽  
Vol 34 (1) ◽  
pp. 169-176 ◽  
Author(s):  
Yifu Zhang
Keyword(s):  
Thermal Stability ◽  
Oxidation Resistance ◽  
Optical Switching ◽  
Variable Temperature ◽  
Thermo Gravimetric Analysis ◽  
Gravimetric Analysis ◽  
Hydrothermal Route ◽  
Scanning Calorimetry ◽  
Good Thermal Stability ◽  
Air Atmosphere

AbstractVanadium dioxide VO2 has been paid in recent years increasing attention because of its various applications, however, its oxidation resistance properties in air atmosphere have rarely been reported. Herein, VO2(B) nanobelts were transformed into VO2(A) and VO2(M) nanobelts by hydrothermal route and calcination treatment, respectively. Then, we comparatively studied the oxidation resistance properties of VO2(B), VO2(A) and VO2(M) nanobelts in air atmosphere by thermo-gravimetric analysis and differential thermal analysis (TGA/DTA). It was found that the nanobelts had good thermal stability and oxidation resistance below 341 °C, 408 °C and 465 °C in air, respectively, indicating that they were stable in air at room temperature. The fierce oxidation of the nanobelts occurred at 426, 507 and 645 °C, respectively. The results showed that the VO2(M) nanobelts had the best thermal stability and oxidation resistance among the others. Furthermore, the phase transition temperatures and optical switching properties of VO2(A) and VO2(M) were studied by differential scanning calorimetry (DSC) and variable temperature infrared spectra. It was found that the VO2(A) and VO2(M) nanobelts had outstanding thermochromic character and optical switching properties.

scholarly journals Thiodiethylene glycol based polyesters: synthesis and thermal characterization

e-Polymers ◽  
2006 ◽  
Vol 6 (1) ◽  
Author(s):  
N. Lotti ◽  
L. Finelli ◽  
M. Messori ◽  
A. Munari
Keyword(s):  
Thermo Gravimetric Analysis ◽  
Crystallization Rate ◽  
Thermal Characterization ◽  
Polymeric Chain ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Good Thermal Stability ◽  
Ether Oxygen ◽  
Thiodiethylene Glycol ◽  
Sulfur Containing

AbstractPoly(thiodiethylene terephthalate) (PSDET), poly(thiodiethylene adipate) (PSDEA), poly(diethylene terephthalate) (PDET) and poly(diethylene adipate) (PDEA) for comparison were synthesized and characterized in terms of chemical structure and molecular weight. The thermal behavior was examined by thermo gravimetric analysis and differential scanning calorimetry. All the polymers showed a good thermal stability, even though lower for the sulfur-containing polyesters. At room temperature they appeared as semicrystalline materials, except PDEA, which was an oil; the effect of substitution of ether oxygen atoms with sulfur ones was a lowering in the Tg value, an increment of melting temperature and an increase of crystallization rate. The results were explained as due to the presence of flexible C-S-C bonds in the polymeric chain.

Synthesis, characterization and corrosion protection properties of polyN-(p-bromophenyl)-2-methacrylamide-co-glycidyl methacrylate on low nickel stainless steel

2011 ◽  
Vol 31 (2-3) ◽  
Author(s):  
Sakvai Mohammed Safiullah ◽  
Deivasigamani Thirumoolan ◽  
Kottur Anver Basha ◽  
K. Mani Govindaraju ◽  
Dhanraj Gopi ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Corrosion Protection ◽  
Glycidyl Methacrylate ◽  
Electrochemical Impedance ◽  
Thermo Gravimetric Analysis ◽  
Gel Permeation Chromatography ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Protection Efficiency ◽  
Ft Ir

Abstract The synthesis of copolymers from different feed ratios of N-(p-bromophenyl)-2- methacrylamide (PBPMA) and glycidyl methacrylate (GMA) was achieved by using free radical solution polymerization technique and characterized using FT-IR, 1H and 13C NMR spectroscopy. The thermal stability of the synthesized copolymers was studied using thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC). The molecular weight of the copolymer is determined by gel permeation chromatography (GPC). The corrosion performances of low nickel stainless steel specimens dip coated with different composition of copolymers were investigated in 0.5 M H2SO4 using potentiodynamic polarization and electrochemical impedance spectroscopic (EIS) techniques. The polarization and impedance measurements showed different corrosion protection efficiency with change in composition of the copolymers. It was found that the corrosion protection properties are owing to the barrier effect of the polymer layer covered on the low nickel stainless steel surfaces. However, it is observed that the mole ratio of PBPMA and GMA plays a major role in the protective nature of the copolymer.

Graphene Nanoplates Filled Nylon 6,6 Nanocomposites, Morphological, Thermal, Mechanical and Solvent Uptake Study

2019 ◽  
Vol 41 (3) ◽  
pp. 388-388
Author(s):  
Khalid Saeed Khalid Saeed ◽  
Tariq Shah and Ahmad Hassan Tariq Shah and Ahmad Hassan
Keyword(s):  
Thermo Gravimetric Analysis ◽  
Neutral Red ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Nucleation Effect ◽  
Morphological Studies ◽  
Solvent Uptake ◽  
Nylon 6,6 ◽  
Dsc Analyses ◽  
Graphene Nanoplates

Effect of graphene nanoplates (GNPs) on the properties of Nylon 6,6 (Nyl 6,6) is investigated in present study. The morphological studies presented that the GNPs were dispersed inside the Nyl 6,6 matrix. The thermo gravimetric analysis (TGA) illustrated that the thermal degradation of nanocomposites samples were started at the range of 350-393 oC, which was appreciably higher than neat Nyl 6,6 (360 oC). The differential scanning calorimetry (DSC) analyses revealed that the crystallization temperature (Tc) of GNPs/Nyl 6,6 increased as increased the addition of GNPs, which might be due to the nucleation effect of GNPs. The mechanical properties of Nyl 6,6 was enhanced by incorporation of GNPs upto the addition of an optimal quantity of filler (5%wt GNPs) into the polymer matrix. The stress yield and Young’s modulus of 5%wt GNPs/Nyl 6,6 was 96.79 and 1.54, N/nm2, respectively. Both Nyl 6,6 and nanocomposites samples were also used for the adsorption of Neutral red chloride (NRC) dye, which significantly remove the dye from the aqueous solution. The neat nylon 6,6 and GNPs (5 and 10 wt%)/Nyl 6,6 adsorbed about 88.49, 93.15, and 93.60% within 2 h, respectively.

Characterization of Novel Solid Dispersions of Moringa oleifera Leaf Powder Using Thermo-Analytical Techniques

Processes ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 2230
Author(s):  
Nontsikelelo Noxolo Tafu ◽  
Victoria A. Jideani
Keyword(s):  
Solid Dispersion ◽  
Moringa Oleifera ◽  
Solid Dispersions ◽  
Intermolecular Hydrogen Bond ◽  
Thermo Gravimetric Analysis ◽  
Analytical Techniques ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Hydrogen Bond Interactions ◽  
Leaf Powder

Moringa oleifera leaf powder (MOLP) has been identified as the most important functional ingredient owing to its rich nutritional profile and healthy effects. The solubility and functional properties of this ingredient can be enhanced through solid dispersion technology. This study aimed to investigate the effects of polyethylene glycols (PEGs) 4000 and 6000 as hydrophilic carriers and solid dispersion techniques (freeze-drying, melting, solvent evaporation, and microwave irradiation) on the crystallinity and thermal stability of solid-dispersed Moringa oleifera leaf powders (SDMOLPs). SDMOLPs were dully characterized using powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC), thermo-gravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FTIR). The PXRD results revealed that the solid dispersions were partially amorphous with strong diffraction peaks at 2θ values of 19° and 23°. The calorimetric and thermogravimetric curves showed that PEGs conferred greater stability on the dispersions. The FTIR studyrevealed the existence of strong intermolecular hydrogen bond interactions between MOLP and PEG functional groups. MOLP solid dispersions may be useful in functional foods and beverages and nutraceutical formulations.

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