Nucleated Thrombocyte Count

SummaryThe natural course of defibrination syndrome caused by Echis colorata venom (ECV) in five patients is reported. All patients developed afibrinogenemia within six hours after the bite. Concomitantly a depression in factor V was recorded. Factor VIII and thrombocyte count in blood were normal in most patients. In the light of the known effects of ECV on blood coagulation in vivo and in vitro it is concluded that the afibrinogenemia is due to intravascular clotting.Four patients had transient renal damage, manifested by oliguria, azotemia, albuminuria and cylindruria, ascribed to microthrombi in the renal glomeruli.After the bite, the natural course was benign, no major bleeding was observed, and all signs of coagulopathy reverted to normal within 7 days. Therefore we recommend no specific treatment for this condition. In the case of heavily bleeding patients, administration of antiserum against ECV and/or heparin should be considered.

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Influence of Certain Drugs on the Adhesiveness of Human, Rat, and Rabbit Blood Platelets in Vitro

Thrombosis and Haemostasis ◽

10.1055/s-0038-1656242 ◽

1965 ◽

Vol 13 (02) ◽

pp. 428-438 ◽

Cited By ~ 2

Author(s):

K Reber ◽

A Studer

Keyword(s):

Sodium Citrate ◽

Blood Platelets ◽

Drug Application ◽

Normal Range ◽

Blood Samples ◽

Serotonin Antagonist ◽

Thrombocyte Count ◽

Particle Count ◽

Rabbit Blood

SummaryThis is a comparative study of the methods described by H. P. Wright and O’Brien for determining the adhesiveness of thrombocytes. An attempt is made to characterize and statistically correlate both techniques. With the aid of a Coulter Counter for thrombocyte counts, a normal range is presented for human, rat, and rabbit blood. Anticoagulants used are sodium citrate and Heparin.The influence of Cocaine and the Serotonin antagonist Ro 3-0837 was studied on these same substrates, to determine a pharmacological interference with results of either Wright’s test or O’Brien’s. Both drugs are found to induce a statistically significant increase in the “thrombocyte count” as compared to the corresponding controls. These effects are not real but to be attributed to an increase in particle count due to thrombocyte fragmentation as a consequence of drug application. There is no evidence for the claim that these drugs decrease the adhesiveness of thrombocytes.Numerical results of both tests often show a high and statistically significant correlation, especially following the addition of Ro 3-0837. Such is not true of individual blood samples to which no drug has been added. Evidentally, both tests are not specific for the same characteristic of normal blood platelets. But, when Ro 3-0837 is added, the breakdown of unstable platelets is induced; and the corresponding increase in count of thrombocyte fragments is expressed by both tests in the same fashion.

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Examination of the Thrombocyte Count in Mink in Connection with Anaemia

Acta Veterinaria Scandinavica ◽

10.1186/bf03548296 ◽

1969 ◽

Vol 10 (1) ◽

pp. 48-56

Author(s):

Arne Helgebostad ◽

Tordis Röstöen Skogan

Keyword(s):

Thrombocyte Count

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Comparison of Thrombocyte Count Between Pediatric Patients With Migraine or Tension-Type Headache: A Retrospective Cohort Study

Journal of Child Neurology ◽

10.1177/0883073819862738 ◽

2019 ◽

Vol 34 (13) ◽

pp. 824-829 ◽

Cited By ~ 1

Author(s):

Aviv Benedick ◽

Avraham Zeharia ◽

Tal Eidlitz Markus

Keyword(s):

Negative Correlation ◽

Pediatric Patients ◽

Tension Type Headache ◽

Thrombocyte Count ◽

Laboratory Parameters ◽

Pediatric Headache ◽

Increased Risk ◽

Migraine Group ◽

Headache Clinic ◽

Type Headache

Hypercoagulability may explain the increased risk of thromboembolic cerebrovascular events in patients with migraine. Thrombocytes play a crucial part in the coagulation process, and some studies have demonstrated hyperaggregation of thrombocytes in adult migraineurs. We aimed to compare thrombocyte count between pediatric patients with migraine or tension-type headache and to evaluate the correlation of thrombocyte count with headache parameters. The electronic database of a tertiary pediatric headache clinic was retrospectively searched for all children and adolescents diagnosed with migraine or tension-type headache in 2016-2018. Data on thrombocyte counts were collected from the medical files and compared between the groups by parametric and nonparametric statistical tests. The cohort included 299 patients, 176 girls (59.0%) and 123 (412.0%) boys, of mean age 12.2 ± 3.4 years; 198 had migraine and 101 had tension-type headache. Among the laboratory parameters evaluated, a significantly lower mean thrombocyte number was found in the migraine group than in the tension-type headache group (282 ± 60 vs 304±71 ×103/μL, P = .004). Within the migraine group, there was a significant negative correlation between the thrombocyte count and the duration of headache attacks in hours ( P < .05). No significant between- or within-group differences were found in other laboratory parameters. The low relative thrombocyte count in pediatric headache clinic patients with migraine and its negative correlation with duration of migraine suggest that migraine may be associated with a different underlying pathogenesis from tension-type headache.

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Chicken Immune Cell Assay to Model Adaptive Immune Responses In Vitro

Animals ◽

10.3390/ani11123600 ◽

2021 ◽

Vol 11 (12) ◽

pp. 3600

Author(s):

Filip Larsberg ◽

Maximilian Sprechert ◽

Deike Hesse ◽

Gudrun A. Brockmann ◽

Susanne Kreuzer-Redmer

Keyword(s):

Cell Culture ◽

Immune Cell ◽

Calf Serum ◽

Feed Additives ◽

Blood Sampling ◽

Thrombocyte Count ◽

Modes Of Action ◽

Optimized Protocol ◽

Chicken Blood

Knowledge about the modes of action of immunomodulating compounds such as pathogens, drugs, or feed additives, e.g., probiotics, gained through controlled but animal-related in vitro systems using primary cultured peripheral blood mononuclear cells (PBMCs) will allow the development of targeted nutrition strategies. Moreover, it could contribute to the prevention of infectious diseases and the usage of antimicrobials, and further promote the health of the animals. However, to our knowledge, a protocol for the isolation of PBMCs with reduced thrombocyte count from chicken blood and subsequent cell culture over several days to assess the effects of immunomodulating compounds is not available. Therefore, we established an optimized protocol for blood sampling and immune cell isolation, culture, and phenotyping for chicken PBMCs. For blood sampling commercial Na–citrate tubes revealed the highest count of vital cells compared to commercial Li–heparin (p < 0.01) and K3EDTA (p < 0.05) tubes. Using combined dextran and ficoll density gradient separation, the thrombocyte count was significantly reduced (p < 0.01) compared to slow-speed centrifugation with subsequent ficoll. For cell culture, the supplementation of RPMI-1640 medium with 10% chicken serum resulted in the lowest relative cell count of thrombocytes compared to fetal calf serum (FCS) (p < 0.05). To validate the ability of the cell culture system to respond to stimuli, concanavalin A (conA) was used as a positive control. The optimized protocol allows the isolation and cultivation of vital PBMCs with reduced thrombocyte count from chicken blood for subsequent investigation of the modes of action of immunomodulating compounds.

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The discrepancy between manual and computerized leukocyte and thrombocyte counts

Paediatrica Indonesiana ◽

10.14238/pi43.3.2003.95-8 ◽

2016 ◽

Vol 43 (3) ◽

pp. 95

Author(s):

Bambang Permono ◽

Retno Asih ◽

I Dewa Gede Ugrasena

Keyword(s):

Gold Standard ◽

Leukocyte Count ◽

Predictive Values ◽

Thrombocyte Count ◽

Randomized Sampling ◽

Manual Group ◽

Manual Examination ◽

Blood Specimens ◽

Sensitivity Specificity ◽

Normal Leukocyte

Background Discrepancy between results of leukocyte and throm-bocyte count by computerized and manual examination may existObjective To determine the discrepancy between computerizedand manual leukocyte and thrombocyte count.Methods The design was a randomized sampling cross sectionalstudy. The blood sample was examined with computerized CellDyn 1400 instrument for the leukocyte and thrombocyte count. Formanual examination, blood smear was performed to measurethrombocyte while leukocyte was measured in Improved Neubauerhemocytometer. The results of computerized examination wereused as gold standard. Sensitivity, specificity, predictive values ofmanual count were calculated. The agreement of Kappa and McNemar test were determinedResults Blood specimens drawn from 100 patients with differentkinds of diagnoses were examined using computerized and manualmethods. In computerized group, 66% had normal leukocyte and55% had normal thrombocyte count. In the manual group, 78% ofsubjects had normal leukocyte and 82% had normal thrombocytecount. From leukocyte examination, the sensitivity of manual countwas 87.9%, specificity was 41.2%, and positive predictive valuewas 74.36 with the agreement of Kappa of 0.32 and Mc Nemarvalue of 0.036. From thrombocyte examination, the sensitivity was96.4%, specificity was 35.6%, and positive predictive value was64.6 with the agreement of Kappa of 0.41 and Mc Nemar value of0.41.Conclusion The result of manual thrombocyte count was in ac-cordance with computerized with the agreement of Kappa of 0.41.On the other hand, there was a discrepancy between manual infavor of computerized leukocyte count with the agreement of Kappaof 0.32

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Determining Postoperative Thrombocyte Reduction Cut Off to Predict 30-Day Mortality in Patients Undergo Coronary Artery Bypass Graft Surgery

Blood ◽

10.1182/blood-2019-125432 ◽

2019 ◽

Vol 134 (Supplement_1) ◽

pp. 4914-4914

Author(s):

Ikhwan Rinaldi ◽

Arif Mansjoer

Keyword(s):

Sensitivity And Specificity ◽

Roc Analysis ◽

Conflicts Of Interest ◽

Artery Bypass ◽

Bypass Graft ◽

Area Under The Curve ◽

Early Mortality ◽

Receiver Operating Curve ◽

Thrombocyte Count ◽

Cabg Surgery

Background There are many factors associated with early mortality after CABG, including postoperative thrombocytopenia (Kertai, 2016). Many factors during CABG surgery, such as administration of heparin or cardio pulmonary bypass during surgery are related to thrombocyte count reduction (Hamid, Akhtar, Naqvi, & Ahsan, 2017; Arepally, 2017). However, it is possible for a post-CABG patient to suffer a significant thrombocyte reduction without reaching the thrombocytopenic state (thrombocyte count <150000/µL). Up to this time, there is still lack of study about association between thrombocyte reduction after surgery and 30-day mortality in patients undergo CABG. This study aim to determine cut off point for postoperative thrombocyte reduction as a predictor of 30-day mortality after CABG surgery. Method This is a retrospective cohort study using medical record of 263 adult patients who underwent CABG surgery in dr. Ciptomangunkusumo National Hospital on 2012-2015. Thrombocyte reduction was determined by substracting preoperative thrombocyte count from postoperative thrombocyte count. Receiver operating curve (ROC) analysis between percentage of thrombocyte reduction and 30-day mortality after surgery was done to obtain the sensitivity and specificity value of a particular degree of thrombocyte reduction. Cut off point was obtained from intersection between sensitivity and specificity value. Result Thirty-day mortality rate after CABG surgery in this study was 11.9%. Cut off point obtained from ROC analysis was 30% with area under the curve (AUC) 0.671. The sensitivity of this cut off point to predict early mortality after CABG surgery was 64.5%, while the specificity was 64.7% Conclusion Thrombocyte reduction more than or equal to 30% can be used as a predictor of 30-day mortality after CABG surgery. Figure Disclosures No relevant conflicts of interest to declare.

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KORELASI ANTARA HITUNG TROMBOSIT DENGAN JUMLAH CD4 PASIEN HIV/AIDS

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v17i2.1023 ◽

2018 ◽

Vol 17 (2) ◽

pp. 102

Author(s):

M. I. Diah Pramudianti ◽

Tahono Tahono

Keyword(s):

Immune Deficiency Syndrome ◽

Deficiency Syndrome ◽

Common Finding ◽

Aids Patients ◽

Cross Sectional ◽

Thrombocyte Count ◽

Immunodeficiency Virus ◽

Cross Sectional Design ◽

Acquired Immune Deficiency ◽

Hiv Aids

The Acquired Immune Deficiency Syndrome (AIDS) is the presence of symptoms caused by Human Immunodeficiency Virus (HIV)which belongs to human retroviruses (retroviridae). Thrombocytopenia is a common finding in patients with HIV infection. HIV infectionmay induce thrombocytopenia through immune and non-immune mechanisms, autoimmune combination and inhibition of plateletproduction. The aim of this study is to analyze the correlation between thrombocyte and CD4 count in HIV/AIDS patients. This studyuses a cross sectional design with a total of 17 patients. The subject of this study is HIV/AIDS patients who came to and examined atVCT clinic, dr. Moewardi Hospital Surakarta. To analyze this result the researchers used Spearman (r) correlation with p<0.05, andconfidence interval 95%. Patients’ median age was 30 (21–49) years, 11 (64.7%) men and 6 (35.3%) women. The subjects with AIDSwere 11 (64.7%), and HIV were 6 (35.3%) patients. The duration of antiretroviral (ARV) was 7.5 (4–20) months in 10 subjects.The median of thrombocyte count was 203 (143–327)×103/μL, CD4 absolute 207 (5.0–734)/μL, and CD4 (% lymphocytes) 13.0(2.0–29.0)%. The thrombocyte count was not correlated with CD4 absolute (r=0.456; p=0.066) and CD4% (r=0.218; p=0.400). InHIV patients, low platelet counts will be the result of a host of problems and complications that are associated with the progressive HIVinfection or its management.

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UJI AKTIVITAS JUS BUAH JAMBU BIJI BERDAGING MERAH (Psidium guajava L.) TERHADAP PENINGKATAN TROMBOSIT PADA MENCIT (Mus musculus L.) YANG DIINDUKSI KLORAMFENIKOL

Jurnal Ilmiah As-Syifaa ◽

10.33096/ja.v10i2.340 ◽

2018 ◽

Vol 10 (2) ◽

pp. 170-178

Author(s):

Cindy Trie Permatasari Hosea ◽

Abdul Wahid Jamaluddin ◽

Yuko Mulyono Adikurniawan

Keyword(s):

Mus Musculus ◽

Psidium Guajava ◽

Thrombocyte Count ◽

Guava Fruit ◽

Life Threatening ◽

Post Hoc ◽

One Way Anova ◽

Hemostatic Disorder

Thrombocytopenia is the most common hemostatic disorder acquired in dogs and can be a life-threatening. Guava fruit and leaves extract are believed to improve thrombocyte counts. The purpose of this study was to prove that administration of guava juice could improve thrombocyte count in mice that have thrombocytopenia after induced by chloramphenicol. We were used 24 mice as samples divided into 4 groups : KS group (given aquades for 12 days), KN group (induced by chloramphenicol for 7 days, then aquades on next 5 days), group P1 (induced by chloramphenicol for 7 days then guava juice 5 g/ml concentration on 5 next day); and group P2 (induced by chloramphenicol for 7 days then guava juice 10 g/ml concentration on the next 5 days). Thrombocyte count was measured three times, before treatment (day 0), after chloramphenicol induction (day 7), and after guava juice (day 12). Data of thrombocyte count was analyzed using one way Anova and Post Hoc LSD test. The results showed that the guava juice (Psidium guajava L.) concentration of 5 g/ml and 10 g/ml had significant effect (p <0,05) to increase platelet numbers in mice after induced by chlorampenicol dose 30mg/30grBW.

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Exploration of prognostic factors for critical COVID-19 patients：a nomogram analysis

10.21203/rs.3.rs-48297/v1 ◽

2020 ◽

Author(s):

Lili Wang ◽

Chun Liu ◽

Zhengquan Wang ◽

Yi Lin ◽

Rui Fan

Keyword(s):

Risk Factors ◽

Peripheral Blood ◽

Leucocyte Count ◽

Multiple Logistic Regression Analysis ◽

Receiver Operating Curve ◽

C Reactive Protein ◽

Survival Group ◽

Peripheral Blood Leucocyte ◽

Thrombocyte Count ◽

Reactive Protein

Abstract To discuss influencing factors on critical COVID-19 patient’s prognosis, construct a basic model and predict their mortality risks. Retrospectively analyzed the general condition and respective laboratory biomarkers of critical patients with duration≥24 h from Feb. 10th, 2020 to Mar. 30th, 2020 to separate them into a survival group and death group based on their clinical features. Multiple logistic regression analysis was performed to assess risk factors for critical COVID-19 patient’s and a nomogram was constructed based on screened risk factors. A receiver operating curve (ROC) was created to evaluate the accuracy of the nomogram. Multi-factor Logistic recovery analysis results show: Age, Peripheral blood leucocyte count,Lymphocyte percentage, Thrombocyte count and Hyper C-reactive protein are single danger factors of critical COVID-19 patient’s mortality risk (p＜0.05). ROC curve indicates Nomogram predictive model AUC is 0.958 (95%CI: 0.923-0.993), which has high predictive value. Findings from this study suggest advanced age, high peripheral blood leucocyte count, high hyper C-reactive protein, low lymphocyte percentage and low thrombocyte count are risk factors of critical COVID-19 patient’s death.The Nomogram model is helpful for timely intervention to reduce the incidence of critical COVID-19 patients.

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